DAPP1 (NM_014395) Human Tagged ORF Clone

CAT#: RC215474

DAPP1 (Myc-DDK-tagged)-Human dual adaptor of phosphotyrosine and 3-phosphoinositides (DAPP1)

Product Images

Circular map for RC215474

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DAPP1 (Cat# RC215474, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAPP1 (Cat# TA810236)(1:2000). Positive lysates LY402326 (100ug) and LC402326 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY402326) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215474 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DAPP1 protein (Cat# TP315474). The protein was produced from HEK293T cells transfected with DAPP1 cDNA clone (Cat# RC215474) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DAPP1
• Synonyms: BAM32
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215474 representing NM_014395
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAGAGCAGAACTTCTAGAAGGGAAGATGAGCACCCAGGATCCCTCAGATCTGTGGAGCAGATCCG
  ATGGAGAGGCTGAGCTGCTCCAGGACTTGGGGTGGTATCACGGCAACCTCACACGCCATGCTGCTGAAGC
  TCTTCTCCTCTCAAATGGATGTGACGGCAGCTACCTTCTGAGGGACAGCAATGAGACCACCGGGCTGTAC
  TCTCTCTCTGTGAGGGCCAAAGATTCTGTTAAACACTTTCATGTTGAATATACTGGATATTCATTTAAAT
  TTGGCTTTAATGAATTCTCATCTTTGAAGGATTTTGTCAAGCATTTTGCAAATCAGCCTTTGATTGGAAG
  CGAGACAGGCACTCTGATGGTTCTAAAACATCCCTACCCAAGAAAAGTGGAAGAACCCTCCATTTATGAA
  TCTGTCCGGGTTCACACAGCAATGCAGACAGGAAGAACAGAAGATGACCTTGTGCCCACAGCACCTTCTC
  TGGGCACCAAAGAAGGTTACCTCACCAAACAGGGAGGCCTGGTCAAGACCTGGAAAACAAGATGGTTTAC
  TCTGCACAGGAATGAACTGAAATACTTCAAAGACCAGATGTCACCAGAACCAATTCGGATCCTAGACCTA
  ACAGAATGTTCAGCTGTACAATTCGATTATTCACAAGAAAGGGTAAACTGTTTTTGTTTGGTATTTCCAT
  TCAGGACATTTTATCTCTGTGCAAAGACCGGAGTAGAAGCTGATGAGTGGATCAAGATATTACGCTGGAA
  ATTGTCACAAATAAGAAAACAGCTCAACCAAGGGGAAGGCACGATCCGATCTCGGTCGTTCATCTTTAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215474 representing NM_014395
  Red=Cloning site Green=Tags(s)
  
  MGRAELLEGKMSTQDPSDLWSRSDGEAELLQDLGWYHGNLTRHAAEALLLSNGCDGSYLLRDSNETTGLY
  SLSVRAKDSVKHFHVEYTGYSFKFGFNEFSSLKDFVKHFANQPLIGSETGTLMVLKHPYPRKVEEPSIYE
  SVRVHTAMQTGRTEDDLVPTAPSLGTKEGYLTKQGGLVKTWKTRWFTLHRNELKYFKDQMSPEPIRILDL
  TECSAVQFDYSQERVNCFCLVFPFRTFYLCAKTGVEADEWIKILRWKLSQIRKQLNQGEGTIRSRSFIFK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014395
• ORF Size: 840 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014395.3
• RefSeq Size: 2953 bp
• RefSeq ORF: 843 bp
• Locus ID: 27071
• UniProt ID: Q9UN19
• Cytogenetics: 4q23
• Domains: SH2, PH
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: B cell receptor signaling pathway
• MW: 32.6 kDa
• Gene Summary: May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.[UniProtKB/Swiss-Prot Function]