SCN3A (NM_006922) Human Tagged ORF Clone

CAT#: RC215453

SCN3A (Myc-DDK-tagged)-Human sodium channel, voltage-gated, type III, alpha subunit (SCN3A), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SCN3A
• Synonyms: DEE62; EIEE62; FFEVF4; NAC3; Nav1.3
• Vector: pCMV6-AC-Myc-DDK
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215453 representing NM_006922
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACAGGCACTGTTGGTACCCCCAGGACCTGAAAGCTTCCGCCTTTTTACTAGAGAATCTCTTGCTG
  CTATCGAAAAACGTGCTGCAGAAGAGAAAGCCAAGAAGCCCAAAAAGGAACAAGATAATGATGATGAGAA
  CAAACCAAAGCCAAATAGTGACTTGGAAGCTGGAAAGAACCTTCCATTTATTTATGGAGACATTCCTCCA
  GAGATGGTGTCAGAGCCCCTGGAGGACCTGGATCCCTACTATATCAATAAGAAAACTTTTATAGTAATGA
  ATAAAGGAAAGGCAATTTTCCGATTCAGTGCCACCTCTGCCTTGTATATTTTAACTCCACTAAACCCTGT
  TAGGAAAATTGCTATCAAGATTTTGGTACATTCTTTATTCAGCATGCTTATCATGTGCACTATTTTGACC
  AACTGTGTATTTATGACCTTGAGCAACCCTCCTGACTGGACAAAGAATGTAGAGTACACATTCACTGGAA
  TCTATACCTTTGAGTCACTTATAAAAATCTTGGCAAGAGGGTTTTGCTTAGAAGATTTTACGTTTCTTCG
  TGATCCATGGAACTGGCTGGATTTCAGTGTCATTGTGATGGCATATGTGACAGAGTTTGTGGACCTGGGC
  AATGTCTCAGCGTTGAGAACATTCAGAGTTCTCCGAGCACTGAAAACAATTTCAGTCATTCCAGGTTTAA
  AGACCATTGTGGGGGCCCTGATCCAGTCGGTAAAGAAGCTTTCTGATGTGATGATCCTGACTGTGTTCTG
  TCTGAGCGTGTTTGCTCTCATTGGGCTGCAGCTGTTCATGGGCAATCTGAGGAATAAATGTTTGCAGTGG
  CCCCCAAGCGATTCTGCTTTTGAAACCAACACCACTTCCTACTTTAATGGCACAATGGATTCAAATGGGA
  CATTTGTTAATGTAACAATGAGCACATTTAACTGGAAGGATTACATTGGAGATGACAGTCACTTTTATGT
  TTTGGATGGGCAAAAAGACCCTTTACTCTGTGGAAATGGCTCAGATGCAGGCCAGTGTCCAGAAGGATAC
  ATCTGTGTGAAGGCTGGTCGAAACCCCAACTATGGCTACACAAGCTTTGACACCTTTAGCTGGGCTTTCC
  TGTCTCTATTTCGACTCATGACTCAAGACTACTGGGAAAATCTTTACCAGTTGACATTACGTGCTGCTGG
  GAAAACATACATGATATTTTTTGTCCTGGTCATTTTCTTGGGCTCATTTTATTTGGTGAATTTGATCCTG
  GCTGTGGTGGCCATGGCCTATGAGGAGCAGAATCAGGCCACCTTGGAAGAAGCAGAACAAAAAGAGGCCG
  AATTTCAGCAGATGCTCGAACAGCTTAAAAAGCAACAGGAAGAAGCTCAGGCAGTTGCGGCAGCATCAGC
  TGCTTCAAGAGATTTCAGTGGAATAGGTGGGTTAGGAGAGCTGTTGGAAAGTTCTTCAGAAGCATCAAAG
  TTGAGTTCCAAAAGTGCTAAAGAATGGAGGAACCGAAGGAAGAAAAGAAGACAGAGAGAGCACCTTGAAG
  GAAACAACAAAGGAGAGAGAGACAGCTTTCCCAAATCCGAATCTGAAGACAGCGTCAAAAGAAGCAGCTT
  CCTTTTCTCCATGGATGGAAACAGACTGACCAGTGACAAAAAATTCTGCTCCCCTCATCAGTCTCTCTTG
  AGTATCCGTGGCTCCCTGTTTTCCCCAAGACGCAATAGCAAAACAAGCATTTTCAGTTTCAGAGGTCGGG
  CAAAGGATGTTGGATCTGAAAATGACTTTGCTGATGATGAACACAGCACATTTGAAGACAGCGAAAGCAG
  GAGAGACTCACTGTTTGTGCCGCACAGACATGGAGAGCGACGCAACAGTAACGTTAGTCAGGCCAGTATG
  TCATCCAGGATGGTGCCAGGGCTTCCAGCAAATGGGAAGATGCACAGCACTGTGGATTGCAATGGTGTGG
  TTTCCTTGGTGGGTGGACCTTCAGCTCTAACGTCACCTACTGGACAACTTCCCCCAGAGGGCACCACCAC
  AGAAACGGAAGTCAGAAAGAGAAGGTTAAGCTCTTACCAGATTTCAATGGAGATGCTGGAGGATTCCTCT
  GGAAGGCAAAGAGCCGTGAGCATAGCCAGCATTCTGACCAACACAATGGAAGAACTTGAAGAATCTAGAC
  AGAAATGTCCGCCATGCTGGTATAGATTTGCCAATGTGTTCTTGATCTGGGACTGCTGTGATGCATGGTT
  AAAAGTAAAACATCTTGTGAATTTAATTGTTATGGATCCATTTGTTGATCTTGCCATCACTATTTGCATT
  GTCTTAAATACCCTCTTTATGGCCATGGAGCACTACCCCATGACTGAGCAATTCAGTAGTGTGTTGACTG
  TAGGAAACCTGGTCTTTACTGGGATTTTCACAGCAGAAATGGTTCTCAAGATCATTGCCATGGATCCTTA
  TTACTATTTCCAAGAAGGCTGGAATATCTTTGATGGAATTATTGTCAGCCTCAGTTTAATGGAGCTTGGT
  CTGTCAAATGTGGAGGGATTGTCTGTACTGCGATCATTCAGACTGCTTAGAGTTTTCAAGTTGGCAAAAT
  CCTGGCCCACACTAAATATGCTAATTAAGATCATTGGCAATTCTGTGGGGGCTCTAGGAAACCTCACCTT
  GGTGTTGGCCATCATCGTCTTCATTTTTGCTGTGGTCGGCATGCAGCTCTTTGGTAAGAGCTACAAAGAA
  TGTGTCTGCAAGATCAATGATGACTGTACGCTCCCACGGTGGCACATGAACGACTTCTTCCACTCCTTCC
  TGATTGTGTTCCGCGTGCTGTGTGGAGAGTGGATAGAGACCATGTGGGACTGTATGGAGGTCGCTGGCCA
  AACCATGTGCCTTATTGTTTTCATGTTGGTCATGGTCATTGGAAACCTTGTGGTTCTGAACCTCTTTCTG
  GCCTTATTGTTGAGTTCATTTAGCTCAGACAACCTTGCTGCTACTGATGATGACAATGAAATGAATAATC
  TGCAGATTGCAGTAGGAAGAATGCAAAAGGGAATTGATTATGTGAAAAATAAGATGCGGGAGTGTTTCCA
  AAAAGCCTTTTTTAGAAAGCCAAAAGTTATAGAAATCCATGAAGGCAATAAGATAGACAGCTGCATGTCC
  AATAATACTGGAATTGAAATAAGCAAAGAGCTTAATTATCTTAGAGATGGGAATGGAACCACCAGTGGTG
  TAGGTACTGGAAGCAGTGTTGAAAAATACGTAATCGATGAAAATGATTATATGTCATTCATAAACAACCC
  CAGCCTCACCGTCACAGTGCCAATTGCTGTTGGAGAGTCTGACTTTGAAAACTTAAATACTGAAGAGTTC
  AGCAGTGAGTCAGAACTAGAAGAAAGCAAAGAGAAATTAAATGCAACCAGCTCATCTGAAGGAAGCACAG
  TTGATGTTGTTCTACCCCGAGAAGGTGAACAAGCTGAAACTGAACCCGAAGAAGACCTTAAACCGGAAGC
  TTGTTTTACTGAAGGATGTATTAAAAAGTTTCCATTCTGTCAAGTAAGTACAGAAGAAGGCAAAGGGAAG
  ATCTGGTGGAATCTTCGAAAAACCTGCTACAGTATTGTTGAGCACAACTGGTTTGAGACTTTCATTGTGT
  TCATGATCCTTCTCAGTAGTGGTGCATTGGCCTTTGAAGATATATACATTGAACAGCGAAAGACTATCAA
  AACCATGCTAGAATATGCTGACAAAGTCTTTACCTATATATTCATTCTGGAAATGCTTCTCAAATGGGTT
  GCTTATGGATTTCAAACATATTTCACTAATGCCTGGTGCTGGCTAGATTTCTTGATCGTTGATGTTTCTT
  TGGTTAGCCTGGTAGCCAATGCTCTTGGCTACTCAGAACTCGGTGCCATCAAATCATTACGGACATTAAG
  AGCTTTAAGACCTCTAAGAGCCTTATCCCGGTTTGAAGGCATGAGGGTGGTTGTGAATGCTCTTGTTGGA
  GCAATTCCCTCTATCATGAATGTGCTGTTGGTCTGTCTCATCTTCTGGTTGATCTTTAGCATCATGGGTG
  TGAATTTGTTTGCTGGCAAGTTCTACCACTGTGTTAACATGACAACGGGTAACATGTTTGACATTAGTGA
  TGTTAACAATTTGAGTGACTGTCAGGCTCTTGGCAAGCAAGCTCGGTGGAAAAACGTGAAAGTAAACTTT
  GATAATGTTGGCGCTGGCTATCTTGCACTGCTTCAAGTGGCCACATTTAAAGGCTGGATGGATATTATGT
  ATGCAGCTGTTGATTCACGAGATGTTAAACTTCAGCCTGTATATGAAGAAAATCTGTACATGTATTTATA
  CTTTGTCATCTTTATCATCTTTGGGTCATTCTTCACTCTGAATCTATTCATTGGTGTCATCATAGATAAC
  TTCAACCAGCAGAAAAAGAAGTTTGGAGGTCAAGACATCTTTATGACAGAGGAACAGAAAAAATATTACA
  ATGCAATGAAGAAACTTGGATCCAAGAAACCTCAGAAACCCATACCTCGCCCAGCAAACAAATTCCAAGG
  AATGGTCTTTGATTTTGTAACCAGACAAGTCTTTGATATCAGCATCATGATCCTCATCTGCCTCAACATG
  GTCACCATGATGGTGGAAACGGATGACCAGGGCAAATACATGACCCTAGTTTTGTCCCGGATCAACCTAG
  TGTTCATTGTTCTGTTCACTGGAGAATTTGTGCTGAAGCTCGTCTCCCTCAGACACTACTACTTCACTAT
  AGGCTGGAACATCTTTGACTTTGTGGTGGTGATTCTCTCCATTGTAGGTATGTTTCTGGCTGAGATGATA
  GAAAAGTATTTTGTGTCCCCTACCTTGTTCCGAGTGATCCGTCTTGCCAGGATTGGCCGAATCCTACGTC
  TGATCAAAGGAGCAAAGGGGATCCGCACGCTGCTCTTTGCTTTGATGATGTCCCTTCCTGCGTTGTTTAA
  CATCGGCCTCCTGCTCTTCCTGGTCATGTTTATCTATGCCATCTTTGGGATGTCCAACTTTGCCTATGTT
  AAAAAGGAAGCTGGAATTGATGACATGTTCAACTTTGAGACCTTTGGCAACAGCATGATCTGCTTGTTCC
  AAATTACAACCTCTGCTGGCTGGGATGGATTGCTAGCACCTATTCTTAATAGTGCACCACCCGACTGTGA
  CCCTGACACAATTCACCCTGGCAGCTCAGTTAAGGGAGACTGTGGGAACCCATCTGTTGGGATTTTCTTT
  TTTGTCAGTTACATCATCATATCCTTCCTGGTTGTGGTGAACATGTACATCGCGGTCATCCTGGAGAACT
  TCAGTGTTGCTACTGAAGAAAGTGCAGAGCCCCTGAGTGAGGATGACTTTGAGATGTTCTATGAGGTTTG
  GGAAAAGTTTGATCCCGATGCGACCCAGTTTATAGAGTTCTCTAAACTCTCTGATTTTGCAGCTGCCCTG
  GATCCTCCTCTTCTCATAGCAAAACCCAACAAAGTCCAGCTTATTGCCATGGATCTGCCCATGGTCAGTG
  GTGACCGGATCCACTGTCTTGATATTTTATTTGCCTTTACAAAGCGTGTTTTGGGTGAGAGTGGAGAGAT
  GGATGCCCTTCGAATACAGATGGAAGACAGGTTTATGGCATCAAACCCCTCCAAAGTCTCTTATGAGCCT
  ATTACAACCACTTTGAAACGTAAACAAGAGGAGGTGTCTGCCGCTATCATTCAGCGTAATTTCAGATGTT
  ATCTTTTAAAGCAAAGGTTAAAAAATATATCAAGTAACTATAACAAAGAGGCAATTAAAGGGAGGATTGA
  CTTACCTATAAAACAAGACATGATTATTGACAAACTAAATGGGAACTCCACTCCAGAAAAAACAGATGGG
  AGTTCCTCTACCACCTCTCCTCCTTCCTATGATAGTGTAACAAAACCAGACAAGGAAAAGTTTGAGAAAG
  ACAAACCAGAAAAAGAAAGCAAAGGAAAAGAGGTCAGAGAAAATCAAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215453 representing NM_006922
  Red=Cloning site Green=Tags(s)
  
  MAQALLVPPGPESFRLFTRESLAAIEKRAAEEKAKKPKKEQDNDDENKPKPNSDLEAGKNLPFIYGDIPP
  EMVSEPLEDLDPYYINKKTFIVMNKGKAIFRFSATSALYILTPLNPVRKIAIKILVHSLFSMLIMCTILT
  NCVFMTLSNPPDWTKNVEYTFTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFSVIVMAYVTEFVDLG
  NVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQW
  PPSDSAFETNTTSYFNGTMDSNGTFVNVTMSTFNWKDYIGDDSHFYVLDGQKDPLLCGNGSDAGQCPEGY
  ICVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDYWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLVNLIL
  AVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEEAQAVAAASAASRDFSGIGGLGELLESSSEASK
  LSSKSAKEWRNRRKKRRQREHLEGNNKGERDSFPKSESEDSVKRSSFLFSMDGNRLTSDKKFCSPHQSLL
  SIRGSLFSPRRNSKTSIFSFRGRAKDVGSENDFADDEHSTFEDSESRRDSLFVPHRHGERRNSNVSQASM
  SSRMVPGLPANGKMHSTVDCNGVVSLVGGPSALTSPTGQLPPEGTTTETEVRKRRLSSYQISMEMLEDSS
  GRQRAVSIASILTNTMEELEESRQKCPPCWYRFANVFLIWDCCDAWLKVKHLVNLIVMDPFVDLAITICI
  VLNTLFMAMEHYPMTEQFSSVLTVGNLVFTGIFTAEMVLKIIAMDPYYYFQEGWNIFDGIIVSLSLMELG
  LSNVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKE
  CVCKINDDCTLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQTMCLIVFMLVMVIGNLVVLNLFL
  ALLLSSFSSDNLAATDDDNEMNNLQIAVGRMQKGIDYVKNKMRECFQKAFFRKPKVIEIHEGNKIDSCMS
  NNTGIEISKELNYLRDGNGTTSGVGTGSSVEKYVIDENDYMSFINNPSLTVTVPIAVGESDFENLNTEEF
  SSESELEESKEKLNATSSSEGSTVDVVLPREGEQAETEPEEDLKPEACFTEGCIKKFPFCQVSTEEGKGK
  IWWNLRKTCYSIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWV
  AYGFQTYFTNAWCWLDFLIVDVSLVSLVANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVG
  AIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNMTTGNMFDISDVNNLSDCQALGKQARWKNVKVNF
  DNVGAGYLALLQVATFKGWMDIMYAAVDSRDVKLQPVYEENLYMYLYFVIFIIFGSFFTLNLFIGVIIDN
  FNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGMVFDFVTRQVFDISIMILICLNM
  VTMMVETDDQGKYMTLVLSRINLVFIVLFTGEFVLKLVSLRHYYFTIGWNIFDFVVVILSIVGMFLAEMI
  EKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYV
  KKEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSAPPDCDPDTIHPGSSVKGDCGNPSVGIFF
  FVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFIEFSKLSDFAAAL
  DPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEDRFMASNPSKVSYEP
  ITTTLKRKQEEVSAAIIQRNFRCYLLKQRLKNISSNYNKEAIKGRIDLPIKQDMIIDKLNGNSTPEKTDG
  SSSTTSPPSYDSVTKPDKEKFEKDKPEKESKGKEVRENQK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_006922
• ORF Size: 6000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006922.4
• RefSeq Size: 9123 bp
• RefSeq ORF: 6003 bp
• Locus ID: 6328
• UniProt ID: Q9NY46
• Cytogenetics: 2q24.3
• Domains: IQ, ion_trans
• Protein Families: Druggable Genome, Ion Channels: Sodium, Transmembrane
• MW: 226.1 kDa
• Gene Summary: Voltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit with 24 transmembrane domains and one or more regulatory beta subunits. They are responsible for the generation and propagation of action potentials in neurons and muscle. This gene encodes one member of the sodium channel alpha subunit gene family, and is found in a cluster of five alpha subunit genes on chromosome 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]