DAP Kinase 1 (DAPK1) (NM_004938) Human Tagged ORF Clone

CAT#: RC215423

DAPK1 (Myc-DDK-tagged)-Human death-associated protein kinase 1 (DAPK1)

Product Images

Circular map for RC215423

• 

  Western blot validation of overexpression lysate (Cat# LY417640) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215423 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified DAPK1 protein (Cat# TP315423). The protein was produced from HEK293T cells transfected with DAPK1 cDNA clone (Cat# RC215423) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DAP Kinase 1
• Synonyms: DAPK; ROCO3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215423 representing NM_004938
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGTGTTCAGGCAGGAAAACGTGGATGATTACTACGACACCGGCGAGGAACTTGGCAGTGGACAGT
  TTGCGGTTGTGAAGAAATGCCGTGAGAAAAGCACCGGCCTCCAGTATGCCGCCAAATTCATCAAGAAAAG
  GAGGACTAAGTCCAGCCGGCGGGGTGTGAGCCGCGAGGACATCGAGCGGGAGGTCAGCATCCTGAAGGAG
  ATCCAGCACCCCAATGTCATCACCCTGCACGAGGTCTATGAGAACAAGACGGACGTCATCCTGATCTTGG
  AACTCGTTGCAGGTGGCGAGCTGTTTGACTTCTTAGCTGAAAAGGAATCTTTAACTGAAGAGGAAGCAAC
  TGAATTTCTCAAACAAATTCTTAATGGTGTTTACTACCTGCACTCCCTTCAAATCGCCCACTTTGATCTT
  AAGCCTGAGAACATAATGCTTTTGGATAGAAATGTCCCCAAACCTCGGATCAAGATCATTGACTTTGGGT
  TGGCCCATAAAATTGACTTTGGAAATGAATTTAAAAACATATTTGGGACTCCAGAGTTTGTCGCTCCTGA
  GATAGTCAACTATGAACCTCTTGGTCTTGAGGCAGATATGTGGAGTATCGGGGTAATAACCTATATCCTC
  CTAAGTGGGGCCTCCCCATTTCTTGGAGACACTAAGCAAGAAACGTTAGCAAATGTATCCGCTGTCAACT
  ACGAATTTGAGGATGAATACTTCAGTAATACCAGTGCCCTAGCCAAAGATTTCATAAGAAGACTTCTGGT
  CAAGGATCCAAAGAAGAGAATGACAATTCAAGATAGTTTGCAGCATCCCTGGATCAAGCCTAAAGATACA
  CAACAGGCACTTAGTAGAAAAGCATCAGCAGTAAACATGGAGAAATTCAAGAAGTTTGCAGCCCGGAAAA
  AATGGAAACAATCCGTTCGCTTGATATCACTGTGCCAAAGATTATCCAGGTCATTCCTGTCCAGAAGTAA
  CATGAGTGTTGCCAGAAGCGATGATACTCTGGATGAGGAAGACTCCTTTGTGATGAAAGCCATCATCCAT
  GCCATCAACGATGACAATGTCCCAGGCCTGCAGCACCTTCTGGGCTCATTATCCAACTATGATGTTAACC
  AACCCAACAAGCACGGGACACCTCCATTACTCATTGCTGCTGGCTGTGGGAATATTCAAATACTACAGTT
  GCTCATTAAAAGAGGCTCGAGAATCGATGTCCAGGATAAGGGCGGGTCCAATGCCGTCTACTGGGCTGCT
  CGGCATGGCCACGTCGATACCTTGAAATTTCTCAGTGAGAACAAATGCCCTTTGGATGTGAAAGACAAGT
  CTGGAGAGATGGCCCTCCACGTGGCAGCTCGCTATGGCCATGCTGACGTGGCTCAGTTACTGTGCAGCTT
  CGGCTCAAATCCCAATATCCAGGACAAGGAAGAAGAAACCCCCCTGCACTGTGCTGCTTGGCACGGCTAT
  TACTCTGTGGCCAAAGCCCTTTGTGAAGCCGGCTGTAACGTGAACATCAAGAACCGAGAAGGAGAGACGC
  CCCTCCTGACAGCCTCTGCCAGGGGCTACCACGACATCGTGGAGTGTCTGGCCGAACATGGAGCCGATCT
  TAATGCTTGCGACAAGGACGGACACATTGCCCTTCATCTGGCTGTAAGACGGTGTCAGATGGAGGTAATC
  AAGACTCTCCTCAGCCAAGGGTGTTTCGTCGATTATCAAGACAGGCACGGCAATACTCCCCTCCATGTGG
  CATGTAAAGATGGCAACATGCCTATCGTGGTGGCCCTCTGTGAAGCAAACTGCAATTTGGACATCTCCAA
  CAAGTATGGGCGAACGCCTCTGCACCTTGCGGCCAACAACGGAATCCTAGACGTGGTCCGGTATCTCTGT
  CTGATGGGAGCCAGCGTTGAGGCGCTGACCACGGACGGAAAGACGGCAGAAGATCTTGCTAGATCGGAAC
  AGCACGAGCACGTAGCAGGTCTCCTTGCAAGACTTCGAAAGGATACGCACCGAGGACTCTTCATCCAGCA
  GCTCCGACCCACACAGAACCTGCAGCCAAGAATTAAGCTCAAGCTGTTTGGCCACTCGGGATCCGGGAAA
  ACCACCCTTGTAGAATCTCTCAAGTGTGGGCTGCTGAGGAGCTTTTTCAGAAGGCGTCGGCCCAGACTGT
  CTTCCACCAACTCCAGCAGGTTCCCACCTTCACCCCTGGCTTCTAAGCCCACAGTCTCAGTGAGCATCAA
  CAACCTGTACCCAGGCTGCGAGAACGTGAGTGTGAGGAGCCGCAGCATGATGTTCGAGCCGGGTCTTACC
  AAAGGGATGCTGGAGGTGTTTGTGGCCCCGACCCACCACCCGCACTGCTCGGCCGATGACCAGTCCACCA
  AGGCCATCGACATCCAGAACGCTTATTTGAATGGAGTTGGCGATTTCAGCGTGTGGGAGTTCTCTGGAAA
  TCCTGTGTATTTCTGCTGTTATGACTATTTTGCTGCAAATGATCCCACGTCAATCCATGTTGTTGTCTTT
  AGTCTAGAAGAGCCCTATGAGATCCAGCTGAACCAAGTGATTTTCTGGCTCAGTTTCCTGAAGTCCCTTG
  TCCCAGTTGAAGAACCCATAGCCTTCGGTGGCAAGCTGAAGAACCCACTCCAAGTTGTCCTGGTGGCCAC
  CCACGCTGACATCATGAATGTTCCTCGACCGGCTGGAGGCGAGTTTGGATATGACAAAGACACATCGTTG
  CTGAAAGAGATTAGGAACAGGTTTGGAAATGATCTTCACATTTCAAATAAGCTGTTTGTTCTGGATGCTG
  GGGCTTCTGGGTCAAAGGACATGAAGGTACTTCGAAATCATCTGCAAGAAATACGAAGCCAGATTGTTTC
  GGTCTGTCCTCCCATGACTCACCTGTGTGAGAAAATCATCTCCACGCTGCCTTCCTGGAGGAAGCTCAAT
  GGACCCAACCAGCTGATGTCGCTGCAGCAGTTTGTGTACGACGTGCAGGACCAGCTGAACCCCCTGGCCA
  GCGAGGAGGACCTCAGGCGCATTGCTCAGCAGCTCCACAGCACAGGCGAGATCAACATCATGCAAAGTGA
  AACAGTTCAGGACGTGCTGCTCCTGGACCCCCGCTGGCTCTGCACAAACGTCCTGGGGAAGTTGCTGTCC
  GTGGAGACCCCACGGGCGCTGCACCACTACCGGGGCCGCTACACCGTGGAGGACATCCAGCGCCTGGTGC
  CCGACAGCGACGTGGAGGAGCTGCTGCAGATCCTCGATGCCATGGACATCTGCGCCCGGGACCTGAGCAG
  CGGGACCATGGTGGACGTCCCAGCCCTGATCAAGACAGACAACCTGCACCGCTCCTGGGCTGATGAGGAG
  GACGAGGTGATGGTGTATGGTGGCGTGCGCATCGTGCCCGTGGAACACCTCACCCCCTTCCCATGTGGCA
  TCTTTCACAAGGTCCAGGTGAACCTGTGCCGGTGGATCCACCAGCAAAGCACAGAGGGCGACGCGGACAT
  CCGCCTGTGGGTGAATGGCTGCAAGCTGGCCAACCGTGGGGCCGAGCTGCTGGTGCTGCTGGTCAACCAC
  GGCCAGGGCATTGAGGTCCAGGTCCGTGGCCTGGAGACGGAGAAGATCAAGTGCTGCCTGCTGCTGGACT
  CGGTGTGCAGCACCATTGAGAACGTCATGGCCACCACGCTGCCAGGGCTCCTGACCGTGAAGCATTACCT
  GAGCCCCCAGCAGCTGCGGGAGCACCATGAGCCCGTCATGATCTACCAGCCACGGGACTTCTTCCGGGCA
  CAGACTCTGAAGGAAACCTCACTGACCAACACCATGGGGGGGTACAAGGAAAGCTTCAGCAGCATCATGT
  GCTTCGGGTGTCACGACGTCTACTCACAGGCCAGCCTCGGCATGGACATCCATGCATCAGACCTGAACCT
  CCTCACTCGGAGGAAACTGAGTCGCCTGCTGGACCCGCCCGACCCCCTGGGGAAGGACTGGTGCCTTCTC
  GCCATGAACTTAGGCCTCCCTGACCTCGTGGCAAAGTACAACACCAATAACGGGGCTCCCAAGGATTTCC
  TCCCCAGCCCCCTCCACGCCCTGCTGCGGGAATGGACCACCTACCCTGAGAGCACAGTGGGCACCCTCAT
  GTCCAAACTGAGGGAGCTGGGTCGCCGGGATGCCGCAGACTTTTTGCTGAAGGCATCCTCTGTGTTCAAA
  ATCAACCTGGATGGCAATGGCCAGGAGGCCTATGCCTCGAGCTGCAACAGCGGCACCTCTTACAATTCCA
  TTAGCTCTGTTGTATCCCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215423 representing NM_004938
  Red=Cloning site Green=Tags(s)
  
  MTVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILKE
  IQHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILNGVYYLHSLQIAHFDL
  KPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYIL
  LSGASPFLGDTKQETLANVSAVNYEFEDEYFSNTSALAKDFIRRLLVKDPKKRMTIQDSLQHPWIKPKDT
  QQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIH
  AINDDNVPGLQHLLGSLSNYDVNQPNKHGTPPLLIAAGCGNIQILQLLIKRGSRIDVQDKGGSNAVYWAA
  RHGHVDTLKFLSENKCPLDVKDKSGEMALHVAARYGHADVAQLLCSFGSNPNIQDKEEETPLHCAAWHGY
  YSVAKALCEAGCNVNIKNREGETPLLTASARGYHDIVECLAEHGADLNACDKDGHIALHLAVRRCQMEVI
  KTLLSQGCFVDYQDRHGNTPLHVACKDGNMPIVVALCEANCNLDISNKYGRTPLHLAANNGILDVVRYLC
  LMGASVEALTTDGKTAEDLARSEQHEHVAGLLARLRKDTHRGLFIQQLRPTQNLQPRIKLKLFGHSGSGK
  TTLVESLKCGLLRSFFRRRRPRLSSTNSSRFPPSPLASKPTVSVSINNLYPGCENVSVRSRSMMFEPGLT
  KGMLEVFVAPTHHPHCSADDQSTKAIDIQNAYLNGVGDFSVWEFSGNPVYFCCYDYFAANDPTSIHVVVF
  SLEEPYEIQLNQVIFWLSFLKSLVPVEEPIAFGGKLKNPLQVVLVATHADIMNVPRPAGGEFGYDKDTSL
  LKEIRNRFGNDLHISNKLFVLDAGASGSKDMKVLRNHLQEIRSQIVSVCPPMTHLCEKIISTLPSWRKLN
  GPNQLMSLQQFVYDVQDQLNPLASEEDLRRIAQQLHSTGEINIMQSETVQDVLLLDPRWLCTNVLGKLLS
  VETPRALHHYRGRYTVEDIQRLVPDSDVEELLQILDAMDICARDLSSGTMVDVPALIKTDNLHRSWADEE
  DEVMVYGGVRIVPVEHLTPFPCGIFHKVQVNLCRWIHQQSTEGDADIRLWVNGCKLANRGAELLVLLVNH
  GQGIEVQVRGLETEKIKCCLLLDSVCSTIENVMATTLPGLLTVKHYLSPQQLREHHEPVMIYQPRDFFRA
  QTLKETSLTNTMGGYKESFSSIMCFGCHDVYSQASLGMDIHASDLNLLTRRKLSRLLDPPDPLGKDWCLL
  AMNLGLPDLVAKYNTNNGAPKDFLPSPLHALLREWTTYPESTVGTLMSKLRELGRRDAADFLLKASSVFK
  INLDGNGQEAYASSCNSGTSYNSISSVVSR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004938
• ORF Size: 4290 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004938.4
• RefSeq Size: 5910 bp
• RefSeq ORF: 4293 bp
• Locus ID: 1612
• UniProt ID: P53355
• Cytogenetics: 9q21.33
• Domains: DEATH, pkinase, TyrKc, ANK, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Bladder cancer, Pathways in cancer
• MW: 159.9 kDa
• Gene Summary: Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]