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CHD8 (NM_020920) Human Tagged ORF Clone
CAT#: RC215369
- TrueORF®
CHD8 (Myc-DDK-tagged)-Human chromodomain helicase DNA binding protein 8 (CHD8), transcript variant 2
ORF Plasmid: tGFP
"NM_020920" in other vectors (2)
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Frequently bought together (3)
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
USD 198.00
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | CHD8 |
| Synonyms | AUTS18; HELSNF1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215369 representing NM_020920
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGGTGAATCGAAACGCATCACCCTGGTCCTCCAGCAGCCACAGTCTGGAGGTCCCCAAGGACATC GGCATGTTGTGCTAGGGAGTCTACCAGGCAAGATAGTGTTACAGGGCAACCAGCTGGCAGCCCTGACTCA AGCCAAGAATGCCCAAGGGCAGCCTGCCAAGGTAGTAACTATCCAGCTGCAGGTGCAGCAGCCACAGCAA AAAATCCAGATTGTACCACAACCACCATCATCGCAGCCACAGCCCCAGCAGCCACCCTCCACCCAGCCAG TGACTCTGTCCTCTGTACAGCAGGCTCAGATAATGGGACCAGGACAAAGCCCAGGACAAAGACTTTCAGT ACCAGTCAAGGTGGTACTGCAGCCACAGGCTGGCTCTTCCCAAGGGGCCTCTTCTGGGCTCTCTGTAGTT AAAGTTCTGAGTGCCAGTGAAGTGGCAGCTTTGTCATCACCAGCAAGCTCTGCTCCTCATTCGGGGGGAA AGACAGGAATGGAGGAAAACCGCAGATTGGAACACCAGAAGAAGCAAGAGAAAGCAAATCGGATTGTAGC AGAGGCCATTGCGAGAGCCCGTGCCCGCGGTGAGCAGAACATACCTCGAGTCTTAAATGAGGACGAGTTG CCCAGCGTTCGGCCAGAGGAGGAAGGCGAGAAGAAACGCAGGAAGAAGAGTGCTGGGGAGAGGCTGAAAG AGGAGAAGCCAAAGAAGAGTAAAACATCTGGTGCCTCCAAAACAAAGGGCAAGAGCAAGCTCAACACCAT CACTCCTGTAGTGGGTAAGAAGAGAAAACGTAATACCTCATCTGATAATTCAGATGTGGAAGTCATGCCT GCACAGTCACCTCGAGAAGATGAAGAAAGCAGCATTCAGAAGAGACGCTCAAACCGCCAAGTTAAGCGAA AAAAATATACAGAGGACCTGGATATAAAGATCACAGATGATGAAGAAGAAGAAGAGGTGGATGTAACTGG TCCAATAAAACCTGAGCCTATCCTCCCTGAACCAGTGCAAGAACCAGATGGCGAGACTTTGCCTTCCATG CAGTTCTTTGTGGAGAATCCCAGTGAAGAAGATGCAGCCATTGTAGACAAAGTGCTTTCTATGCGGATTG TGAAGAAGGAGCTCCCTTCTGGACAATATACTGAAGCAGAAGAATTCTTTGTCAAGTACAAGAACTACTC CTATCTGCATTGTGAATGGGCTACTATCTCCCAACTAGAGAAGGATAAGAGGATACATCAAAAATTAAAG CGCTTCAAAACCAAAATGGCTCAGATGAGACACTTCTTCCATGAGGATGAAGAGCCCTTTAATCCAGACT ACGTAGAGGTGGATAGGATATTGGATGAGTCTCACAGTATTGACAAGGACAATGGGGAGCCCGTTATTTA CTACCTGGTGAAATGGTGCTCTCTGCCCTATGAGGATAGCACATGGGAGCTAAAAGAAGATGTTGATGAG GGCAAGATTCGAGAATTTAAACGGATTCAGTCAAGGCACCCAGAACTCAAAAGGGTGAATCGTCCGCAGG CAAGTGCCTGGAAGAAATTGGAGCTATCACATGAATATAAAAACAGAAACCAGCTACGGGAATATCAGTT GGAAGGGGTTAATTGGCTGCTCTTTAATTGGTATAACAGGCAGAACTGCATCCTGGCTGATGAGATGGGA TTGGGCAAAACTATTCAGTCCATTGCCTTCTTGCAGGAAGTATATAATGTGGGCATCCATGGTCCCTTCT TGGTCATTGCCCCACTGTCCACAATTACTAACTGGGAGCGAGAATTTAATACATGGACAGAAATGAACAC TATTGTGTACCATGGCAGTCTGGCCAGCAGGCAGATGATTCAACAGTATGAAATGTACTGCAAAGATTCA CGGGGACGCCTCATCCCAGGCGCATACAAGTTTGACGCTCTGATCACCACTTTTGAGATGATTTTGTCAG ATTGTCCTGAGCTTCGTGAAATTGAATGGCGTTGTGTTATCATTGATGAAGCCCATCGACTGAAAAACCG TAATTGCAAGCTGCTTGATAGTCTCAAGCACATGGACCTGGAACACAAGGTGCTACTCACAGGAACACCA TTGCAAAATACTGTAGAAGAACTGTTTAGCTTGCTTCATTTCTTGGAACCGTCACAATTTCCCTCAGAAT CAGAGTTTCTCAAGGACTTTGGGGATCTCAAGACAGAGGAACAGGTTCAAAAGCTACAGGCCATTCTTAA GCCAATGATGCTGAGAAGACTCAAAGAGGATGTTGAAAAAAACTTGGCACCCAAACAGGAAACAATTATT GAAGTAGAGCTGACTAATATCCAGAAGAAATACTATCGGGCTATTTTGGAGAAGAATTTCTCCTTCCTTT CCAAAGGGGCAGGTCATACCAACATGCCTAATCTACTTAACACAATGATGGAGTTGCGCAAGTGCTGCAA CCACCCATATCTCATCAATGGTGCTGAAGAAAAAATCCTAACAGAATTCCGTGAAGCTTGCCATATTATA CCTCATGACTTTCACCTGCAGGCCATGGTTCGTTCAGCCGGCAAACTGGTTCTTATTGACAAGTTGCTTC CAAAGCTTAAAGCTGGTGGCCATAAAGTTCTGATCTTCTCTCAGATGGTGCGCTGCCTAGACATCCTAGA GGATTATTTAATCCAGAGGAGGTACTTATATGAACGTATTGATGGGCGAGTTAGAGGCAACCTTCGACAG GCTGCCATTGACCGCTTCAGCAAGCCTGACTCAGACCGCTTTGTCTTCTTACTGTGTACCCGGGCTGGTG GACTTGGTATTAATCTTACAGCTGCTGATACCTGCATCATCTTTGATTCAGACTGGAATCCACAAAATGA CCTGCAGGCCCAGGCACGATGTCATCGAATTGGGCAGAGCAAAGCTGTGAAGGTGTACCGCCTCATCACT CGTAATTCCTACGAGAGAGAGATGTTTGATAAGGCCAGCCTCAAGTTGGGGTTGGATAAGGCTGTGCTTC AATCCATGAGTGGTCGGGATGGCAACATTACTGGAATCCAACAGTTCTCTAAGAAGGAGATTGAAGATCT TTTAAGAAAAGGAGCATATGCAGCCATCATGGAGGAAGATGATGAAGGCTCCAAGTTTTGTGAAGAGGAC ATTGACCAGATCTTGTTAAGACGAACTACAACCATCACCATTGAATCTGAAGGAAAAGGTTCCACCTTTG CTAAGGCAAGCTTTGTTGCTTCTGAAAACAGGACAGATATTTCTTTGGATGACCCCAACTTTTGGCAAAA GTGGGCCAAAAAGGCTGACCTAGACATGGATCTGCTCAACAGCAAGAATAATTTGGTAATTGACACACCT AGAGTACGAAAACAAACGCGCCACTTTAGCACTCTGAAAGATGATGACCTGGTGGAATTCTCTGATTTGG AAAGTGAGGATGATGAGCGGCCACGCTCCCGCAGACATGACCGTCATCATGCCTATGGGCGCACTGACTG CTTTCGGGTGGAAAAGCATCTCCTGGTATATGGTTGGGGACGATGGCGAGATATTTTATCTCATGGACGC TTCAAGCGACGTATGACTGAACGAGATGTGGAGACCATTTGTCGGGCCATTCTCGTGTACTGTCTTCTAC ACTACCGTGGGGATGAAAATATTAAAGGCTTCATCTGGGACTTGATTAGCCCAGCTGAAAATGGCAAGAC AAAAGAATTGCAGAATCATTCAGGTCTATCTATCCCTGTGCCTCGTGGACGCAAAGGAAAAAAAGTAAAG TCACAAAGCACTTTTGATATCCATAAGGCAGATTGGATCCGGAAATATAACCCTGACACTTTGTTCCAAG ATGAAAGTTATAAGAAGCACTTGAAACATCAGTGTAACAAGGTACTGTTGCGGGTACGAATGCTATACTA CCTGAGGCAGGAGGTTATTGGAGACCAAGCAGAAAAGGTGTTAGGGGGTGCGATTGCCAGTGAGATTGAC ATATGGTTCCCAGTAGTGGATCAACTGGAGGTTCCAACAACTTGGTGGGACAGTGAGGCTGACAAGTCGC TGCTCATTGGAGTCTTTAAACATGGCTATGAGAAATATAATACCATGAGGGCAGACCCAGCCTTATGTTT CCTAGAAAAGGCTGGCCGACCAGATGACAAAGCAATTGCAGCAGAACATCGAGTGTTGGATAACTTCTCT GACATAGTAGAAGGGGTTGACTTTGATAAAGATTGTGAAGATCCTGAATATAAACCACTCCAAGGTCCCC CAAAGGACCAAGATGATGAGGGTGATCCCTTGATGATGATGGATGAGGAGATCTCAGTGATTGATGGAGA TGAAGCCCAGGTGACCCAACAGCCAGGCCATTTATTCTGGCCTCCGGGCTCTGCCCTAACAGCTAGGCTT CGGCGTCTAGTAACAGCGTATCAGCGCAGCTACAAGAGAGAACAAATGAAGATAGAGGCTGCAGAACGTG GGGACCGGCGAAGGCGGCGTTGTGAAGCAGCCTTCAAGCTGAAAGAAATTGCACGGCGGGAGAAACAACA ACGATGGACAAGGCGTGAACAAACTGATTTTTATCGAGTGGTGTCTACGTTTGGTGTGGAATATGACCCT GACACCATGCAGTTCCATTGGGATCGCTTCCGCACTTTTGCTCGACTAGACAAAAAGACAGATGAAAGCC TTACCAAGTACTTCCATGGCTTTGTGGCCATGTGCCGCCAAGTATGCCGCCTTCCCCCAGCAGCTGGAGA TGAACCCCCCGACCCTAACCTGTTCATTGAGCCCATCACTGAGGAGAGAGCCTCACGGACTCTCTACCGT ATAGAATTGCTTCGGCGCTTACGGGAACAAGTTTTATGCCACCCCCTTTTGGAAGATCGGCTGGCATTGT GTCAGCCTCCTGGTCCTGAATTGCCCAAATGGTGGGAGCCTGTTCGGCATGATGGGGAGCTTCTAAGAGG GGCAGCCCGCCATGGGGTGAGCCAAACAGACTGCAACATCATGCAGGACCCAGACTTCTCTTTTCTGGCT GCCCGTATGAATTATATGCAGAACCATCAAGCAGGAGCACCAGCTCCATCCTTGTCACGCTGCTCTACTC CACTGCTGCACCAGCAGTATACCTCACGCACTGCCTCACCACTGCCCCTGCGCCCAGATGCTCCTGTTGA AAAGTCACCCGAGGAGACAGCTACCCAGGTCCCCAGTCTGGAGAGTCTGACTTTAAAGCTAGAGCACGAG GTGGTGGCCAGGAGCCGACCAACCCCACAAGACTATGAGATGCGAGTATCCCCCTCTGATACTACCCCTC TGGTTTCCCGGAGTGTTCCACCAGTCAAACTGGAGGATGAGGATGATTCGGACTCTGAGCTGGACTTGAG CAAGCTGTCACCATCTTCTTCTTCTTCCTCATCCTCATCCAGCTCCAGCTCCAGCACTGATGAGAGTGAG GATGAGAAGGAAGAGAAGCTAACTGACCAGTCCCGCTCAAAGCTCTATGATGAAGAGAGTCTCCTGTCCC TCACTATGTCCCAAGATGGATTCCCAAATGAAGATGGAGAACAAATGACCCCTGAGCTTCTGCTACTGCA GGAAAGACAAAGAGCCTCTGAGTGGCCCAAGGATCGTGTCCTGATAAACCGTATTGACCTCGTCTGCCAG GCTGTACTCTCAGGGAAGTGGCCTTCTAGCCGTAGGAGCCAGGAAATGGTAACAGGAGGAATTTTGGGGC CAGGCAACCACTTGCTAGACAGTCCCTCATTGACTCCTGGAGAATATGGTGACTCTCCAGTCCCCACACC ACGAAGTAGTAGTGCAGCTTCCATGGCAGAGGAGGAAGCATCTGCAGTCAGCACAGCGGCAGCCCAGTTC ACCAAACTTCGCCGAGGCATGGATGAAAAGGAGTTTACAGTTCAAATCAAAGATGAGGAAGGATTGAAGT TAACATTCCAGAAGCACAAGTTGATGGCGAATGGAGTAATGGGAGATGGACATCCACTGTTTCATAAGAA GAAGGGGAACAGAAAGAAGCTAGTAGAGCTGGAGGTGGAGTGCATGGAAGAGCCTAATCACCTTGATGTG GACCTGGAGACCCGGATCCCTGTCATCAATAAGGTGGATGGTACTTTGCTGGTGGGTGAGGATGCCCCTC GCCGGGCTGAACTGGAGATGTGGTTACAGGGTCATCCAGAGTTTGCTGTTGATCCCCGATTTCTAGCGTA TATGGAGGATCGCAGAAAACAGAAGTGGCAAAGATGTAAAAAAAATAATAAGGCAGAATTGAACTGTTTG GGAATGGAACCAGTACAGACAGCTAACTCTAGAAATGGGAAAAAGGGTCATCACACTGAAACGGTGTTCA ACCGGGTTTTGCCAGGGCCTATTGCACCAGAGAGCAGCAAGAAGCGGGCCCGTAGGATGCGACCAGACCT TTCTAAGATGATGGCCCTCATGCAGGGTGGAAGCACTGGGTCTCTATCTCTGCATAACACGTTCCAACAC AGCAGTAGTGGCCTACAGTCTGTGTCATCTTTGGGTCACAGCAGTGCCACTTCTGCATCTTTGCCTTTTA TGCCATTTGTGATGGGTGGTGCACCATCATCCCCTCATGTAGACTCCAGCACCATGCTTCATCACCACCA CCACCACCCCCACCCCCACCATCACCACCATCACCATCCAGGCTTGAGAGCCCCTGGCTACCCCTCTTCA CCAGTGACTACCGCCTCTGGTACTACCTTGCGGTTGCCACCACTGCAACCTGAGGAGGATGACGATGAGG ATGAAGAAGATGATGATGACTTATCTCAGGGCTATGATAGCTCAGAAAGGGACTTCTCACTCATTGATGA TCCTATGATGCCAGCTAACTCAGACTCCAGTGAAGATGCTGATGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215369 representing NM_020920
Red=Cloning site Green=Tags(s) MKGESKRITLVLQQPQSGGPQGHRHVVLGSLPGKIVLQGNQLAALTQAKNAQGQPAKVVTIQLQVQQPQQ KIQIVPQPPSSQPQPQQPPSTQPVTLSSVQQAQIMGPGQSPGQRLSVPVKVVLQPQAGSSQGASSGLSVV KVLSASEVAALSSPASSAPHSGGKTGMEENRRLEHQKKQEKANRIVAEAIARARARGEQNIPRVLNEDEL PSVRPEEEGEKKRRKKSAGERLKEEKPKKSKTSGASKTKGKSKLNTITPVVGKKRKRNTSSDNSDVEVMP AQSPREDEESSIQKRRSNRQVKRKKYTEDLDIKITDDEEEEEVDVTGPIKPEPILPEPVQEPDGETLPSM QFFVENPSEEDAAIVDKVLSMRIVKKELPSGQYTEAEEFFVKYKNYSYLHCEWATISQLEKDKRIHQKLK RFKTKMAQMRHFFHEDEEPFNPDYVEVDRILDESHSIDKDNGEPVIYYLVKWCSLPYEDSTWELKEDVDE GKIREFKRIQSRHPELKRVNRPQASAWKKLELSHEYKNRNQLREYQLEGVNWLLFNWYNRQNCILADEMG LGKTIQSIAFLQEVYNVGIHGPFLVIAPLSTITNWEREFNTWTEMNTIVYHGSLASRQMIQQYEMYCKDS RGRLIPGAYKFDALITTFEMILSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTP LQNTVEELFSLLHFLEPSQFPSESEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETII EVELTNIQKKYYRAILEKNFSFLSKGAGHTNMPNLLNTMMELRKCCNHPYLINGAEEKILTEFREACHII PHDFHLQAMVRSAGKLVLIDKLLPKLKAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNLRQ AAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLIT RNSYEREMFDKASLKLGLDKAVLQSMSGRDGNITGIQQFSKKEIEDLLRKGAYAAIMEEDDEGSKFCEED IDQILLRRTTTITIESEGKGSTFAKASFVASENRTDISLDDPNFWQKWAKKADLDMDLLNSKNNLVIDTP RVRKQTRHFSTLKDDDLVEFSDLESEDDERPRSRRHDRHHAYGRTDCFRVEKHLLVYGWGRWRDILSHGR FKRRMTERDVETICRAILVYCLLHYRGDENIKGFIWDLISPAENGKTKELQNHSGLSIPVPRGRKGKKVK SQSTFDIHKADWIRKYNPDTLFQDESYKKHLKHQCNKVLLRVRMLYYLRQEVIGDQAEKVLGGAIASEID IWFPVVDQLEVPTTWWDSEADKSLLIGVFKHGYEKYNTMRADPALCFLEKAGRPDDKAIAAEHRVLDNFS DIVEGVDFDKDCEDPEYKPLQGPPKDQDDEGDPLMMMDEEISVIDGDEAQVTQQPGHLFWPPGSALTARL RRLVTAYQRSYKREQMKIEAAERGDRRRRRCEAAFKLKEIARREKQQRWTRREQTDFYRVVSTFGVEYDP DTMQFHWDRFRTFARLDKKTDESLTKYFHGFVAMCRQVCRLPPAAGDEPPDPNLFIEPITEERASRTLYR IELLRRLREQVLCHPLLEDRLALCQPPGPELPKWWEPVRHDGELLRGAARHGVSQTDCNIMQDPDFSFLA ARMNYMQNHQAGAPAPSLSRCSTPLLHQQYTSRTASPLPLRPDAPVEKSPEETATQVPSLESLTLKLEHE VVARSRPTPQDYEMRVSPSDTTPLVSRSVPPVKLEDEDDSDSELDLSKLSPSSSSSSSSSSSSSSTDESE DEKEEKLTDQSRSKLYDEESLLSLTMSQDGFPNEDGEQMTPELLLLQERQRASEWPKDRVLINRIDLVCQ AVLSGKWPSSRRSQEMVTGGILGPGNHLLDSPSLTPGEYGDSPVPTPRSSSAASMAEEEASAVSTAAAQF TKLRRGMDEKEFTVQIKDEEGLKLTFQKHKLMANGVMGDGHPLFHKKKGNRKKLVELEVECMEEPNHLDV DLETRIPVINKVDGTLLVGEDAPRRAELEMWLQGHPEFAVDPRFLAYMEDRRKQKWQRCKKNNKAELNCL GMEPVQTANSRNGKKGHHTETVFNRVLPGPIAPESSKKRARRMRPDLSKMMALMQGGSTGSLSLHNTFQH SSSGLQSVSSLGHSSATSASLPFMPFVMGGAPSSPHVDSSTMLHHHHHHPHPHHHHHHHPGLRAPGYPSS PVTTASGTTLRLPPLQPEEDDDEDEEDDDDLSQGYDSSERDFSLIDDPMMPANSDSSEDADD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020920 |
| ORF Size | 6906 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020920.4 |
| RefSeq Size | 7499 bp |
| RefSeq ORF | 6909 bp |
| Locus ID | 57680 |
| UniProt ID | Q9HCK8 |
| Cytogenetics | 14q11.2 |
| Protein Pathways | Wnt signaling pathway |
| MW | 262.3 kDa |
| Gene Summary | This gene encodes a member of the chromodomain-helicase-DNA binding protein family, which is characterized by a SNF2-like domain and two chromatin organization modifier domains. The encoded protein also contains brahma and kismet domains, which are common to the subfamily of chromodomain-helicase-DNA binding proteins to which this protein belongs. This gene has been shown to function in several processes that include transcriptional regulation, epigenetic remodeling, promotion of cell proliferation, and regulation of RNA synthesis. Allelic variants of this gene are associated with autism spectrum disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2016] |
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| SDS |
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