ACK1 (TNK2) (NM_001010938) Human Tagged ORF Clone

CAT#: RC215366

TNK2 (Myc-DDK-tagged)-Human tyrosine kinase, non-receptor, 2 (TNK2), transcript variant 2

Product Images

Circular map for RC215366

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ACK1
• Synonyms: ACK; ACK-1; ACK1; p21cdc42Hs
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215366 representing NM_001010938
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCGAGGCCCGGCCCCCCAGGACGCAGGGCAGTGACGCTGCCGGTGCCGCTGCGGGGCGGGGGCTGC
  GGGCGCTGCTTCTCTCCCTGACCGCAGCCGCTGGGATTTGGGGCTCCATGGGGGAGAGATCTGCTTACCA
  GCGCCTGGCTGGGGGCGAGGAGGGACCGCAGAGGCTGGGAGGCGGCAGAATGCAGCCAGAGGAGGGCACA
  GGCTGGCTGCTGGAGCTGCTGTCCGAGGTGCAGCTGCAACAGTACTTCCTGCGGCTCCGAGATGACCTCA
  ACGTCACCCGCCTGTCCCACTTTGAGTACGTCAAGAATGAGGACCTGGAGAAGATCGGCATGGGTCGGCC
  TGGCCAGCGGCGGCTGTGGGAGGCTGTGAAGAGGAGGAAGGCCTTGTGCAAACGCAAGTCGTGGATGAGT
  AAGGTGTTCAGTGGAAAGCGACTGGAGGCTGAGTTCCCACCTCATCACTCTCAGAGCACCTTCCGGAAGA
  CCTCGCCCGCCCCTGGGGGCCCAGCAGGGGAGGGGCCCCTGCAGAGCCTCACCTGCCTCATTGGGGAGAA
  GGACCTGCGCCTCCTGGAGAAGCTGGGTGATGGTTCCTTTGGCGTGGTGCGCAGGGGCGAGTGGGACGCG
  CCCTCAGGGAAGACGGTGAGTGTGGCTGTGAAGTGCCTGAAGCCCGATGTCCTGAGCCAGCCAGAAGCCA
  TGGACGACTTCATCCGGGAGGTCAATGCCATGCACTCGCTCGACCACCGAAACCTCATCCGCCTCTACGG
  GGTGGTGCTCACGCCGCCCATGAAGATGGTGACAGAGCTGGCACCTCTGGGATCGTTGTTGGACCGGCTA
  CGTAAGCACCAGGGCCACTTCCTCCTGGGGACTCTGAGCCGCTACGCTGTGCAGGTGGCTGAGGGCATGG
  GCTACCTGGAGTCCAAGCGCTTTATTCACCGTGACCTGGCTGCCCGCAATCTGCTGTTGGCTACCCGCGA
  CCTGGTCAAGATCGGGGACTTTGGGCTGATGCGAGCACTACCTCAGAATGACGACCATTACGTCATGCAG
  GAACATCGCAAGGTGCCCTTCGCCTGGTGTGCCCCCGAGAGCCTGAAGACACGCACCTTCTCCCATGCCA
  GCGACACCTGGATGTTCGGGGTGACACTGTGGGAAATGTTCACCTACGGCCAGGAGCCCTGGATCGGCCT
  CAACGGCAGTCAGATCCTGCATAAGATCGACAAGGAGGGGGAGCGGCTGCCCCGGCCCGAGGACTGTCCC
  CAGGACATCTACAACGTCATGGTCCAGTGCTGGGCTCACAAGCCAGAGGACAGACCCACGTTTGTGGCCC
  TGCGGGACTTCCTGCTGGAGGCCCAGCCCACAGACATGCGGGCCCTTCAGGACTTTGAGGAACCGGACAA
  GCTGCACATCCAGATGAATGATGTCATCACCGTCATCGAGGGAAGGGCCGAGAACTACTGGTGGCGTGGC
  CAGAACACACGGACGCTGTGTGTGGGGCCCTTCCCTCGCAACGTGGTGACCTCCGTGGCCGGCCTGTCGG
  CCCAGGACATCAGCCAGCCCCTGCAGAACAGCTTCATCCACACAGGGCATGGCGACAGTGACCCCCGCCA
  CTGCTGGGGCTTCCCGGACAGGATTGACGAACTGTATCTGGGAAACCCCATGGACCCCCCCGACCTCCTG
  AGCGTGGAACTGAGCACCTCCCGGCCCCCCCAGCATCTAGGAGGGGTGAAAAGGGAGCCTCCACCTCGCC
  CACCTCAGCCTGCCTTCTTCACTCAGAAACCAACCTATGACCCTGTGAGCGAGGACCAAGACCCCTTGTC
  CAGCGACTTCAAGAGGCTGGGCCTGCGGAAGCCAGGCCTGCCCCGAGGGCTGTGGCTGGCGAAGCCCTCG
  GCGCGGGTGCCGGGCACCAAGGCCAGCCGAGGCAGCGGGGCTGAGGTCACGCTCATCGACTTCGGTGAGG
  AGCCCGTGGTCCCGGCCCTACGGCCCTGCGCGCCCTCCCTGGCGCAGCTGGCCATGGACGCCTGCTCCCT
  GCTGGACGAGACCCCGCCTCAGAGCCCCACGCGGGCACTGCCCCGGCCCCTGCACCCCACGCCTGTGGTG
  GACTGGGACGCACGCCCGCTGCCCCCCCCGCCCGCCTATGACGACGTGGCCCAGGATGAGGATGACTTTG
  AGATCTGCTCCATCAACAGCACCCTCGTGGGCGCGGGGGTCCCTGCCGGGCCCAGCCAGGGCCAGACCAA
  CTACGCCTTTGTGCCTGAGCAGGCGCGGCCGCCCCCTCCCCTGGAGGACAACCTGTTCCTCCCGCCCCAG
  GGTGGGGGCAAGCCGCCCAGCTCCGCACAGACCGCAGAGATCTTCCAGGCGCTACAGCAGGAGTGCATGA
  GGCAACTGCAGGCTCCGGCCGGCTCCCCGGCCCCCTCTCCCAGCCCGGGGGGTGACGACAAGCCCCAGGT
  GCCTCCTCGGGTACCCATCCCCCCTCGGCCCACGCGCCCACACGTCCAGCTGTCTCCAGCCCCCCCGGGC
  GAGGAGGAGACCAGCCAGTGGCCTGGACCTGCTTCCCCTCCCCGGGTGCCTCCGCGGGAGCCCCTGTCCC
  CTCAAGGCTCGAGGACACCCAGCCCCCTGGTACCACCTGGCAGCTCCCCGCTGCCACCCCGGCTCTCAAG
  CTCACCTGGGAAGACCATGCCCACCACCCAGAGCTTTGCCTCAGACCCCAAGTACGCCACCCCCCAGGTG
  ATCCAGGCCCCTGGCCCGCGGGCTGGTCCCTGCATCCTGCCCATCGTCCGGGATGGCAAGAAGGTCAGCA
  GCACCCACTATTACTTGCTGCCCGAGCGACCATCCTACCTGGAGCGCTACCAGCGCTTCCTGCGTGAGGC
  CCAGAGCCCCGAGGAGCCTACCCCCCTGCCTGTGCCTCTGCTGCTGCCCCCACCCAGCACCCCAGCCCCC
  GCCGCCCCCACGGCCACCGTGCGGCCGATGCCCCAGGCTGCCTTGGACCCCAAGGCCAACTTCTCCACCA
  ACAACAGCAACCCAGGGGCCCGGCCACCACCCCCGAGGGCCACTGCTCGGCTGCCACAGAGGGGCTGCCC
  TGGCGATGGGCCAGAGGCGGGCCGGCCAGCAGACAAGATCCAGATGGTGGAGCAGCTCTTCGGGCTGGGT
  CTGCGGCCCAGAGGGGAGTGCCACAAAGTGCTGGAGATGTTCGACTGGAACCTGGAGCAGGCCGGCTGCC
  ACCTTCTGGGCTCCTGGGGCCCTGCCCACCACAAGCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215366 representing NM_001010938
  Red=Cloning site Green=Tags(s)
  
  MLEARPPRTQGSDAAGAAAGRGLRALLLSLTAAAGIWGSMGERSAYQRLAGGEEGPQRLGGGRMQPEEGT
  GWLLELLSEVQLQQYFLRLRDDLNVTRLSHFEYVKNEDLEKIGMGRPGQRRLWEAVKRRKALCKRKSWMS
  KVFSGKRLEAEFPPHHSQSTFRKTSPAPGGPAGEGPLQSLTCLIGEKDLRLLEKLGDGSFGVVRRGEWDA
  PSGKTVSVAVKCLKPDVLSQPEAMDDFIREVNAMHSLDHRNLIRLYGVVLTPPMKMVTELAPLGSLLDRL
  RKHQGHFLLGTLSRYAVQVAEGMGYLESKRFIHRDLAARNLLLATRDLVKIGDFGLMRALPQNDDHYVMQ
  EHRKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTYGQEPWIGLNGSQILHKIDKEGERLPRPEDCP
  QDIYNVMVQCWAHKPEDRPTFVALRDFLLEAQPTDMRALQDFEEPDKLHIQMNDVITVIEGRAENYWWRG
  QNTRTLCVGPFPRNVVTSVAGLSAQDISQPLQNSFIHTGHGDSDPRHCWGFPDRIDELYLGNPMDPPDLL
  SVELSTSRPPQHLGGVKREPPPRPPQPAFFTQKPTYDPVSEDQDPLSSDFKRLGLRKPGLPRGLWLAKPS
  ARVPGTKASRGSGAEVTLIDFGEEPVVPALRPCAPSLAQLAMDACSLLDETPPQSPTRALPRPLHPTPVV
  DWDARPLPPPPAYDDVAQDEDDFEICSINSTLVGAGVPAGPSQGQTNYAFVPEQARPPPPLEDNLFLPPQ
  GGGKPPSSAQTAEIFQALQQECMRQLQAPAGSPAPSPSPGGDDKPQVPPRVPIPPRPTRPHVQLSPAPPG
  EEETSQWPGPASPPRVPPREPLSPQGSRTPSPLVPPGSSPLPPRLSSSPGKTMPTTQSFASDPKYATPQV
  IQAPGPRAGPCILPIVRDGKKVSSTHYYLLPERPSYLERYQRFLREAQSPEEPTPLPVPLLLPPPSTPAP
  AAPTATVRPMPQAALDPKANFSTNNSNPGARPPPPRATARLPQRGCPGDGPEAGRPADKIQMVEQLFGLG
  LRPRGECHKVLEMFDWNLEQAGCHLLGSWGPAHHKR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001010938
• ORF Size: 3258 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001010938.1, NP_001010938.1
• RefSeq Size: 4222 bp
• RefSeq ORF: 3144 bp
• Locus ID: 10188
• UniProt ID: Q07912
• Cytogenetics: 3q29
• Protein Families: Druggable Genome, Protein Kinase
• MW: 119.35 kDa
• Gene Summary: This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008]