NBR1 (NM_031862) Human Tagged ORF Clone

CAT#: RC215342

NBR1 (Myc-DDK-tagged)-Human neighbor of BRCA1 gene 1 (NBR1), transcript variant 3

Product Images

Circular map for RC215342

• 

  Western blot validation of overexpression lysate (Cat# LY410463) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222234 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NBR1
• Synonyms: 1A1-3B; IAI3B; M17S2; MIG19
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215342 representing NM_031862
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCACAGGTTACTCTAAATGTGACTTTTAAAAATGAAATTCAAAGCTTTCTGGTTTCTGATCCAG
  AAAATACAACTTGGGCTGATATCGAAGCTATGGTAAAAGTTTCATTTGATCTGAATACTATTCAAATAAA
  ATACCTGGATGAGGAAAATGAAGAGGTATCCATCAACAGTCAAGGAGAATATGAAGAAGCGCTTAAGATG
  GCAGTTAAACAGGGAAACCAACTGCAGATGCAAGTCCACGAAGGGCACCATGTCGTTGATGAAGCCCCAC
  CCCCAGTTGTAGGAGCAAAACGACTAGCTGCCAGGGCAGGGAAGAAGCCACTTGCACATTACTCTTCACT
  GGTGAGAGTCTTGGGATCAGACATGAAGACCCCAGAGGATCCTGCAGTGCAGTCGTTTCCACTTGTTCCA
  TGTGACACAGACCAGCCTCAAGACAAGCCCCCAGACTGGTTCACAAGCTACCTGGAGACGTTCAGAGAAC
  AAGTGGTTAACGAAACGGTTGAGAAGCTTGAACAGAAATTACATGAAAAGCTTGTCCTCCAGAACCCATC
  CTTGGGTTCTTGTCCCTCAGAAGTCTCAATGCCTACTTCAGAAGAAACATTGTTTTTGCCAGAAAACCAG
  TTCAGCTGGCATATTGCTTGCAACAACTGCCAAAGAAGGATTGTTGGTGTCCGCTACCAGTGTAGCCTAT
  GCCCATCCTACAATATCTGTGAAGATTGTGAAGCAGGGCCATATGGCCATGACACTAACCACGTCCTGCT
  GAAGTTGCGGAGACCTGTTGTGGGCTCCTCTGAACCGTTCTGTCACTCAAAGTACTCTACTCCTCGTCTT
  CCTGCTGCTCTGGAACAAGTCAGGCTCCAGAAACAGGTTGATAAGAACTTTCTTAAAGCAGAAAAGCAAA
  GGTTGCGAGCTGAGAAGAAACAACGTAAAGCAGAGGTCAAGGAACTTAAAAAGCAGCTTAAACTCCATAG
  GAAAATTCACCTGTGGAATTCAATCCATGGACTCCAGAGCCCCAAGTCTCCTTTAGGCCGACCTGAGAGC
  TTGCTCCAGTCTAATACCCTGATGCTCCCTTTGCAGCCCTGTACCTCCGTTATGCCAATGCTCAGTGCAG
  CATTTGTGGATGAGAATTTGCCTGATGGGACTCACCTTCAGCCAGGAACCAAGTTTATCAAACACTGGAG
  GATGAAAAATACAGGAAATGTAAAGTGGAGTGCAGACACAAAGCTCAAGTTCATGTGGGGAAACCTGACT
  TTGGCTTCCACAGAAAAGAAGGATGTTTTGGTTCCCTGCCTCAAGGCCGGCCATGTGGGAGTTGTATCTG
  TGGAGTTCATTGCCCCAGCCTTGGAGGGAACGTATACTTCCCATTGGCGTCTTTCTCACAAAGGCCAGCA
  ATTTGGGCCTCGGGTCTGGTGCAGTATCATAGTAGATCCTTTCCCCTCCGAAGAGAGCCCTGATAACATT
  GAAAAGGGCATGATCAGCTCAAGCAAAACTGATGATCTCACCTGCCAGCAAGAGGAAACTTTTCTTCTGG
  CTAAAGAAGAAAGACAGCTTGGTGAAGTGACTGAGCAGACAGAAGGGACAGCAGCCTGCATCCCACAGAA
  GGCAAAAAATGTTGCCAGTGAGAGGGAGCTCTACATCCCATCTGTGGATCTTCTGACTGCCCAGGACCTG
  CTGTCCTTTGAGCTGTTGGATATAAACATTGTTCAAGAGTTGGAGAGAGTGCCCCACAACACCCCTGTGG
  ATGTGACTCCCTGCATGTCTCCTCTGCCACATGACAGTCCTTTAATAGAGAAGCCAGGCTTGGGGCAGAT
  AGAGGAAGAGAATGAAGGGGCAGGATTTAAAGCACTTCCTGATTCTATGGTGTCAGTAAAGAGGAAGGCT
  GAGAACATTGCTTCTGTGGAGGAAGCAGAAGAAGACCTGAGTGGGACCCAGTTTGTGTGTGAGACAGTAA
  TCCGATCCCTTACCTTGGATGCTGCCCCAGACCACAACCCTCCTTGCAGACAGAAGTCCTTGCAGATGAC
  ATTTGCCTTGCCTGAAGGACCACTTGGAAATGAGAAGGAGGAGATTATCCATATCGCTGAGGAAGAAGCT
  GTCATGGAGGAGGAGGAGGATGAGGAGGATGAGGAGGAGGAGGATGAGCTCAAAGATGAAGTTCAAAGTC
  AGTCCTCTGCTTCCTCAGAGGATTACATCATCATCCTGCCTGAGTGCTTTGATACCAGCCGCCCCCTGGG
  GGATTCTATGTACAGCTCTGCGCTCTCACAGCCAGGCCTGGAGCGAGGTGCTGAAGGCAAGCCTGGGGTT
  GAGGCTGGGCAGGAACCAGCTGAGGCTGGGGAAAGACTCCCTGGAGGGGAGAACCAGCCACAGGAGCACA
  GCATAAGTGACATCCTCACGACCTCACAGACTCTGGAAACAGTGCCCCTAATCCCAGAGGTAGTGGAGCT
  TCCACCGTCACTGCCCAGGAGCTCTCCTTGTGTACATCATCATGGTTCCCCAGGAGTGGATTTACCAGTT
  ACCATACCAGAAGTTTCTTCAGTCCCTGATCAGATCAGAGGAGAGCCCAGAGGCTCATCAGGACTTGTAA
  ACAGCAGACAGAAGAGCTATGACCACTCAAGGCACCATCATGGGAGCAGCATTGCTGGAGGACTGGTGAA
  GGGGGCTTTGTCTGTTGCTGCCTCTGCATACAAGGCCCTGTTTGCTGGGCCACCAGTCACTGCACAGCCA
  ATAATTTCTGAAGATCAGACAGCAGCCCTGATGGCCCATCTCTTTGAAATGGGATTCTGTGACAGGCAGC
  TGAACCTACGGCTGCTGAAGAAACACAATTACAATATCCTGCAGGTTGTGACAGAACTTCTTCAGTTAAA
  CAACAACGACTGGTACAGCCAACGCTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215342 representing NM_031862
  Red=Cloning site Green=Tags(s)
  
  MEPQVTLNVTFKNEIQSFLVSDPENTTWADIEAMVKVSFDLNTIQIKYLDEENEEVSINSQGEYEEALKM
  AVKQGNQLQMQVHEGHHVVDEAPPPVVGAKRLAARAGKKPLAHYSSLVRVLGSDMKTPEDPAVQSFPLVP
  CDTDQPQDKPPDWFTSYLETFREQVVNETVEKLEQKLHEKLVLQNPSLGSCPSEVSMPTSEETLFLPENQ
  FSWHIACNNCQRRIVGVRYQCSLCPSYNICEDCEAGPYGHDTNHVLLKLRRPVVGSSEPFCHSKYSTPRL
  PAALEQVRLQKQVDKNFLKAEKQRLRAEKKQRKAEVKELKKQLKLHRKIHLWNSIHGLQSPKSPLGRPES
  LLQSNTLMLPLQPCTSVMPMLSAAFVDENLPDGTHLQPGTKFIKHWRMKNTGNVKWSADTKLKFMWGNLT
  LASTEKKDVLVPCLKAGHVGVVSVEFIAPALEGTYTSHWRLSHKGQQFGPRVWCSIIVDPFPSEESPDNI
  EKGMISSSKTDDLTCQQEETFLLAKEERQLGEVTEQTEGTAACIPQKAKNVASERELYIPSVDLLTAQDL
  LSFELLDINIVQELERVPHNTPVDVTPCMSPLPHDSPLIEKPGLGQIEEENEGAGFKALPDSMVSVKRKA
  ENIASVEEAEEDLSGTQFVCETVIRSLTLDAAPDHNPPCRQKSLQMTFALPEGPLGNEKEEIIHIAEEEA
  VMEEEEDEEDEEEEDELKDEVQSQSSASSEDYIIILPECFDTSRPLGDSMYSSALSQPGLERGAEGKPGV
  EAGQEPAEAGERLPGGENQPQEHSISDILTTSQTLETVPLIPEVVELPPSLPRSSPCVHHHGSPGVDLPV
  TIPEVSSVPDQIRGEPRGSSGLVNSRQKSYDHSRHHHGSSIAGGLVKGALSVAASAYKALFAGPPVTAQP
  IISEDQTAALMAHLFEMGFCDRQLNLRLLKKHNYNILQVVTELLQLNNNDWYSQRY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031862
• ORF Size: 2898 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031862.4
• RefSeq Size: 4654 bp
• RefSeq ORF: 2901 bp
• Locus ID: 4077
• UniProt ID: Q14596
• Cytogenetics: 17q21.31
• Domains: PB1, ZnF_ZZ
• Protein Families: Druggable Genome
• MW: 107.2 kDa
• Gene Summary: The protein encoded by this gene was originally identified as an ovarian tumor antigen monitored in ovarian cancer. The encoded protein contains a B-box/coiled-coil motif, which is present in many genes with transformation potential. It functions as a specific autophagy receptor for the selective autophagic degradation of peroxisomes by forming intracellular inclusions with ubiquitylated autophagic substrates. This gene is located on a region of chromosome 17q21.1 that is in close proximity to the BRCA1 tumor suppressor gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2014]