Growth Arrest Specific Protein 7 (GAS7) (NM_003644) Human Tagged ORF Clone

CAT#: RC215256

GAS7 (Myc-DDK-tagged)-Human growth arrest-specific 7 (GAS7), transcript variant a

Product Images

Circular map for RC215256

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GAS7 (Cat# RC215256, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAS7(Cat# TA501907). Positive lysates LY418527 (100ug) and LC418527 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY418527) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215256 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GAS7 protein (Cat# TP315256). The protein was produced from HEK293T cells transfected with GAS7 cDNA clone (Cat# RC215256) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Growth Arrest Specific Protein 7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215256 representing NM_003644
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAATATGGAGAACAGCTTTGACGATGTTTCTTGCCTCTCTCCCCAGAACCTGGGATCCTCATCGC
  CAAGCAAAAAGCAGAGCAAGGAAAACACCATCACAATAAACTGTGTGACGTTCCCTCACCCAGACACGAT
  GCCGGAACAGCAGCTGCTGAAACCAACCGAGTGGAGCTACTGCGACTACTTCTGGGCTGATAAGAAGGAC
  CCCCAAGGCAACGGCACCGTGGCTGGGTTTGAACTACTGCTCCAGAAACAGCTGAAGGGCAAACAAATGC
  AGAAGGAAATGTCAGAATTCATCCGGGAAAGGATAAAGATTGAAGAAGACTATGCGAAGAACTTAGCTAA
  GCTCTCTCAGAACTCCTTGGCTTCACAGGAGGAAGGCTCCTTGGGAGAGGCGTGGGCCCAGGTGAAGAAG
  AGCCTGGCGGACGAAGCAGAAGTTCACCTCAAGTTCTCTGCCAAGCTTCACAGCGAGGTGGAGAAGCCCC
  TGATGAACTTCCGTGAGAACTTCAAGAAAGACATGAAGAAGTGCGACCACCACATTGCCGACCTTCGCAA
  GCAGCTCGCCAGCCGCTATGCCTCGGTGGAGAAGGCCCGGAAAGCCCTCACAGAGCGGCAGAGAGACCTG
  GAGATGAAGACCCAGCAGCTGGAGATCAAGCTGAGCAACAAGACAGAGGAGGACATCAAGAAGGCGCGGA
  GAAAGTCCACACAGGCTGGAGACGACCTCATGCGCTGTGTGGATCTCTACAACCAGGCCCAGTCCAAATG
  GTTTGAAGAGATGGTGACCACCACATTGGAGCTAGAGCGGCTGGAGGTGGAGAGGGTAGAGATGATCCGG
  CAGCACCTGTGCCAGTACACGCAGCTGCGGCATGAAACAGACATGTTCAACCAAAGCACAGTCGAGCCCG
  TGGATCAGCTGCTTCGAAAAGTGGACCCGGCCAAAGACAGGGAGCTGTGGGTCAGAGAGCACAAGACGGG
  CAACATCCGCCCTGTGGACATGGAGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215256 representing NM_003644
  Red=Cloning site Green=Tags(s)
  
  MSNMENSFDDVSCLSPQNLGSSSPSKKQSKENTITINCVTFPHPDTMPEQQLLKPTEWSYCDYFWADKKD
  PQGNGTVAGFELLLQKQLKGKQMQKEMSEFIRERIKIEEDYAKNLAKLSQNSLASQEEGSLGEAWAQVKK
  SLADEAEVHLKFSAKLHSEVEKPLMNFRENFKKDMKKCDHHIADLRKQLASRYASVEKARKALTERQRDL
  EMKTQQLEIKLSNKTEEDIKKARRKSTQAGDDLMRCVDLYNQAQSKWFEEMVTTTLELERLEVERVEMIR
  QHLCQYTQLRHETDMFNQSTVEPVDQLLRKVDPAKDRELWVREHKTGNIRPVDMEI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003644
• ORF Size: 1008 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003644.3
• RefSeq Size: 7773 bp
• RefSeq ORF: 1011 bp
• Locus ID: 8522
• UniProt ID: O60861
• Cytogenetics: 17p13.1
• Domains: FCH
• Protein Families: Transcription Factors
• MW: 39 kDa
• Gene Summary: Growth arrest-specific 7 is expressed primarily in terminally differentiated brain cells and predominantly in mature cerebellar Purkinje neurons. GAS7 plays a putative role in neuronal development. Several transcript variants encoding proteins which vary in the N-terminus have been described. [provided by RefSeq, Jul 2008]