EBF3 (NM_001005463) Human Tagged ORF Clone

CAT#: RC215222

EBF3 (Myc-DDK-tagged)-Human early B-cell factor 3 (EBF3)

Product Images

Circular map for RC215222

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  Western blot validation of overexpression lysate (Cat# LY423723) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215222 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified EBF3 protein (Cat# TP315222). The protein was produced from HEK293T cells transfected with EBF3 cDNA clone (Cat# RC215222) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EBF3
• Synonyms: COE3; EBF-3; HADDS; O/E-2; OE-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215222 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGGGATTCAGGAGAATATTCCGCGCGGGGGGACGACCATGAAGGAGGAGCCGCTGGGCAGCGGCA
  TGAACCCGGTGCGCTCGTGGATGCACACGGCGGGCGTGGTGGACGCCAACACGGCCGCCCAGAGCGGCGT
  GGGGCTGGCGCGGGCGCACTTCGAGAAGCAGCCGCCTTCCAACCTCCGGAAATCCAATTTCTTCCACTTC
  GTGCTGGCGCTCTACGATAGGCAGGGGCAGCCGGTGGAGATTGAAAGGACCGCTTTTGTGGACTTTGTGG
  AGAAAGAGAAAGAGCCAAACAACGAGAAAACCAACAACGGCATCCACTATAAACTCCAGTTATTGTACAG
  CAACGGAGTCAGAACAGAGCAAGATCTGTATGTTCGCCTCATAGATTCAATGACCAAACAGGCCATCGTC
  TACGAGGGCCAGGACAAGAACCCGGAGATGTGCCGTGTGCTGCTGACCCACGAGATCATGTGCAGCCGGT
  GCTGTGACAAGAAAAGTTGTGGCAATAGAAACGAAACGCCCTCAGACCCTGTAATCATTGACAGATTCTT
  TCTAAAGTTTTTCCTCAAGTGCAATCAGAACTGTTTGAAGAATGCAGGCAACCCTCGAGATATGCGGAGA
  TTCCAGGTTGTTGTATCGACAACAGTCAACGTGGACGGCCACGTGCTGGCCGTGTCAGACAACATGTTTG
  TGCACAACAATTCCAAACACGGGAGGCGGGCCCGCCGCCTAGACCCGTCAGAAGCCACTCCGTGCATCAA
  GGCCATCAGTCCCAGTGAAGGCTGGACCACGGGGGGTGCCACCGTCATCATAATTGGCGACAACTTCTTT
  GACGGGCTGCAAGTTGTATTCGGAACTATGTTGGTGTGGAGCGAGCTGATAACTCCCCATGCCATCCGAG
  TCCAGACCCCGCCGAGGCACATTCCTGGCGTCGTCGAAGTGACCCTCTCCTACAAATCCAAGCAGTTCTG
  CAAAGGTGCTCCTGGGCGCTTTGTCTACACCGCCCTTAATGAACCAACCATAGATTACGGCTTTCAGAGG
  TTGCAGAAAGTGATCCCAAGACATCCGGGTGATCCCGAAAGGTTACCCAAGGAGGTGTTACTGAAGCGGG
  CGGCGGACCTGGTGGAAGCCTTATACGGAATGCCTCACAACAACCAGGAGATCATCTTGAAGCGAGCGGC
  GGACATCGCCGAGGCGCTGTACAGCGTTCCCCGCAATCACAACCAGATCCCCACCCTGGGCAACAACCCT
  GCACACACGGGCATGATGGGCGTCAACTCCTTCAGCAGCCAGCTAGCCGTCAACGTGTCAGAGACGTCAC
  AAGCCAACGACCAAGTCGGCTACAGTCGCAATACAAGCAGCGTGTCCCCGCGAGGCTACGTCCCCAGCAG
  TACTCCCCAGCAGTCCAATTACAACACAGTCAGCACTAGCATGAATGGATATGGAAGTGGCGCCATGGCC
  AGTCTAGGGGTCCCTGGCTCGCCTGGATTTCTTAATGGCTCCTCCGCTAACTCTCCCTACGGCATGAAAC
  AGAAGAGCGCCTTCGCGCCCGTGGTCCGGCCCCAAGCCTCTCCTCCTCCTTCCTGCACCAGCGCCAACGG
  GAATGGACTGCAAGCTATGTCTGGGCTGGTAGTCCCGCCAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215222 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFGIQENIPRGGTTMKEEPLGSGMNPVRSWMHTAGVVDANTAAQSGVGLARAHFEKQPPSNLRKSNFFHF
  VLALYDRQGQPVEIERTAFVDFVEKEKEPNNEKTNNGIHYKLQLLYSNGVRTEQDLYVRLIDSMTKQAIV
  YEGQDKNPEMCRVLLTHEIMCSRCCDKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRR
  FQVVVSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEATPCIKAISPSEGWTTGGATVIIIGDNFF
  DGLQVVFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGAPGRFVYTALNEPTIDYGFQR
  LQKVIPRHPGDPERLPKEVLLKRAADLVEALYGMPHNNQEIILKRAADIAEALYSVPRNHNQIPTLGNNP
  AHTGMMGVNSFSSQLAVNVSETSQANDQVGYSRNTSSVSPRGYVPSSTPQQSNYNTVSTSMNGYGSGAMA
  SLGVPGSPGFLNGSSANSPYGMKQKSAFAPVVRPQASPPPSCTSANGNGLQAMSGLVVPPM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005463
• ORF Size: 1653 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005463.3
• RefSeq Size: 4412 bp
• RefSeq ORF: 1656 bp
• Locus ID: 253738
• UniProt ID: Q9H4W6
• Cytogenetics: 10q26.3
• Protein Families: Transcription Factors
• MW: 60.4 kDa
• Gene Summary: This gene encodes a member of the early B-cell factor (EBF) family of DNA binding transcription factors. EBF proteins are involved in B-cell differentiation, bone development and neurogenesis, and may also function as tumor suppressors. The encoded protein inhibits cell survival through the regulation of genes involved in cell cycle arrest and apoptosis, and aberrant methylation or deletion of this gene may play a role in multiple malignancies including glioblastoma multiforme and gastric carcinoma. [provided by RefSeq, Sep 2011]