ST18 (NM_014682) Human Tagged ORF Clone

CAT#: RC215211

ST18 (Myc-DDK-tagged)-Human suppression of tumorigenicity 18 (breast carcinoma) (zinc finger protein) (ST18)

Product Images

Western blot validation of overexpression lysate (Cat# LY415116) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215211 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ST18
• Synonyms: NZF-3; NZF3; ZC2H2C3; ZC2HC10; ZNF387
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215211 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCAGAGGCTGAAGATAAAACGCTGCGTACTCGCTCTAAAGGAACCGAGGTGCCAATGGATTCAC
  TAATCCAGGAGCTCAGTGTTGCCTATGATTGCTCCATGGCAAAGAAGAGAACAGCTGAAGATCAGGCTTT
  GGGGGTTCCAGTCAACAAAAGGAAATCCCTGCTAATGAAGCCCCGACACTACAGCCCAAAAGCAGACTGC
  CAAGAAGACCGCAGTGACAGGACAGAGGACGATGGCCCCTTGGAAACACATGGTCACTCTACCGCAGAGG
  AAATCATGATAAAACCTATGGATGAAAGTCTTCTTTCAACTGCACAAGAAAACTCCAGTAGGAAGGAAGA
  CAGATACTCTTGTTATCAAGAGCTCATGGTCAAGTCTTTAATGCACTTGGGGAAATTTGAAAAAAATGTA
  TCTGTTCAGACTGTAAGTGAAAATTTAAATGACAGTGGCATCCAGTCTTTAAAAGCAGAGAGCGATGAAG
  CAGACGAGTGCTTTCTGATTCATTCTGATGATGGAAGAGACAAGATTGATGATTCTCAGCCACCCTTCTG
  CTCCTCTGATGACAATGAAAGTAACTCTGAAAGTGCAGAAAATGGCTGGGACAGTGGCTCCAACTTCTCA
  GAAGAAACCAAACCACCTAGAGTCCCAAAGTATGTTTTAACAGATCATAAAAAAGACCTATTGGAAGTTC
  CTGAAATAAAAACTGAAGGTGACAAATTTATCCCTTGTGAGAACAGGTGTGATTCTGAAACAGAAAGGAA
  AGACCCGCAGAATGCTCTCGCAGAACCCCTGGATGGCAATGCCCAGCCCTCATTCCCTGACGTTGAGGAG
  GAAGATAGCGAGAGCCTGGCAGTAATGACGGAAGAGGGTAGTGACCTGGAAAAGGCCAAGGGGAATTTAA
  GTTTGCTGGAGCAGGCAATTGCTCTGCAGGCTGAGCGAGGTTGTGTTTTCCATAACACCTACAAAGAGCT
  GGATAGGTTCCTGCTGGAGCACCTAGCAGGGGAAAGGAGGCAAACCAAAGTTATCGACATGGGTGGAAGA
  CAAATCTTTAACAATAAACATTCACCAAGGCCTGAAAAGAGGGAGACCAAGTGCCCGATCCCTGGATGTG
  ATGGCACGGGACACGTGACAGGGCTCTACCCGCACCACCGCAGCCTTTCGGGGTGCCCCCACAAAGTGCG
  GGTTCCCCTGGAAATTCTTGCCATGCATGAAAATGTGCTCAAGTGTCCCACGCCGGGATGCACAGGAAGG
  GGTCATGTGAACAGCAACCGCAACACCCACAGGAGTCTTTCTGGTTGTCCAATTGCTGCAGCTGAAAAAT
  TGGCAATGTCCCAGGATAAAAATCAGCTTGATTCTCCCCAAACTGGGCAGTGTCCTGACCAGGCCCACAG
  GACAAGTTTGGTGAAGCAAATTGAATTCAATTTCCCGTCACAAGCCATCACCTCTCCCAGAGCCACAGTG
  TCAAAAGAACAAGAGAAGTTTGGAAAAGTACCATTTGATTATGCCAGTTTTGATGCCCAAGTTTTCGGTA
  AACGCCCTCTCATACAAACAGTGCAAGGACGAAAAACACCACCATTTCCTGAATCAAAGCATTTTCCAAA
  TCCAGTGAAATTTCCTAATCGACTGCCTAGTGCAGGCGCCCACACCCAGAGCCCTGGCCGTGCCAGCTCT
  TATAGCTACGGTCAATGTAGTGAAGACACCCACATAGCAGCAGCTGCTGCCATCCTGAACCTTTCCACCC
  GCTGCAGGGAAGCCACAGACATCCTCTCCAACAAGCCACAGAGTCTGCATGCCAAGGGAGCCGAAATAGA
  AGTGGATGAAAATGGCACATTGGACTTAAGCATGAAAAAAAATCGAATCCTGGACAAGTCTGCACCCCTA
  ACTTCCTCTAACACTTCTATTCCAACTCCTTCCTCTTCCCCATTCAAAACAAGCAGCATTCTGGTCAATG
  CAGCATTCTATCAGGCTCTTTGTGACCAAGAGGGCTGGGACACTCCTATCAACTATAGCAAAACTCACGG
  GAAGACAGAGGAGGAGAAAGAGAAAGACCCAGTGAGCTCTCTAGAAAATTTAGAGGAAAAAAAGTTTCCT
  GGAGAGGCCTCTATACCAAGCCCTAAACCCAAGCTTCATGCAAGAGATCTCAAAAAGGAACTAATCACCT
  GTCCAACACCAGGATGTGATGGAAGTGGCCACGTGACAGGAAACTATGCATCTCATCGCAGTGTTTCTGG
  ATGTCCTTTAGCAGATAAGACTCTAAAATCCCTCATGGCTGCCAACTCTCAGGAGCTTAAGTGTCCAACC
  CCAGGCTGCGATGGCTCGGGGCACGTGACTGGAAACTATGCTTCCCACAGAAGCTTGTCCGGATGCCCTC
  GTGCAAGGAAAGGTGGTGTCAAAATGACCCCTACCAAGGAAGAAAAAGAAGACCCTGAACTGAAATGTCC
  TGTGATAGGGTGTGATGGCCAAGGTCACATATCAGGTAAATACACATCACACCGCACAGCTTCTGGCTGT
  CCTCTGGCTGCCAAGAGACAGAAGGAGAATCCTCTCAATGGAGCCTCCCTCTCCTGGAAACTGAACAAAC
  AAGAGCTACCACATTGTCCCTTGCCAGGCTGCAATGGGCTGGGCCATGTAAATAATGTTTTTGTCACCCA
  CCGAAGCTTATCTGGATGTCCTCTCAATGCACAAGTTATCAAAAAGGGCAAGGTTTCTGAAGAACTCATG
  ACCATCAAGCTCAAAGCAACTGGGGGAATAGAGAGTGATGAAGAAATTAGGCATTTGGATGAAGAAATAA
  AGGAACTGAATGAATCCAACCTTAAAATTGAAGCAGATATGATGAAACTTCAGACCCAGATCACATCTAT
  GGAGAGCAACTTAAAGACGATAGAGGAGGAGAACAAACTCATAGAACAGAACAATGAAAGTCTGCTGAAA
  GAGCTGGCAGGTCTAAGCCAAGCTCTCATTTCAAGCCTTGCTGACATCCAGCTTCCACAGATGGGACCTA
  TCAGTGAGCAGAATTTTGAAGCATATGTAAATACACTCACAGATATGTACAGCAATCTGGAACGGGACTA
  TTCCCCGGAATGCAAAGCTCTACTGGAAAGTATCAAACAGGCAGTGAAGGGTATCCATGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215211 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDAEAEDKTLRTRSKGTEVPMDSLIQELSVAYDCSMAKKRTAEDQALGVPVNKRKSLLMKPRHYSPKADC
  QEDRSDRTEDDGPLETHGHSTAEEIMIKPMDESLLSTAQENSSRKEDRYSCYQELMVKSLMHLGKFEKNV
  SVQTVSENLNDSGIQSLKAESDEADECFLIHSDDGRDKIDDSQPPFCSSDDNESNSESAENGWDSGSNFS
  EETKPPRVPKYVLTDHKKDLLEVPEIKTEGDKFIPCENRCDSETERKDPQNALAEPLDGNAQPSFPDVEE
  EDSESLAVMTEEGSDLEKAKGNLSLLEQAIALQAERGCVFHNTYKELDRFLLEHLAGERRQTKVIDMGGR
  QIFNNKHSPRPEKRETKCPIPGCDGTGHVTGLYPHHRSLSGCPHKVRVPLEILAMHENVLKCPTPGCTGR
  GHVNSNRNTHRSLSGCPIAAAEKLAMSQDKNQLDSPQTGQCPDQAHRTSLVKQIEFNFPSQAITSPRATV
  SKEQEKFGKVPFDYASFDAQVFGKRPLIQTVQGRKTPPFPESKHFPNPVKFPNRLPSAGAHTQSPGRASS
  YSYGQCSEDTHIAAAAAILNLSTRCREATDILSNKPQSLHAKGAEIEVDENGTLDLSMKKNRILDKSAPL
  TSSNTSIPTPSSSPFKTSSILVNAAFYQALCDQEGWDTPINYSKTHGKTEEEKEKDPVSSLENLEEKKFP
  GEASIPSPKPKLHARDLKKELITCPTPGCDGSGHVTGNYASHRSVSGCPLADKTLKSLMAANSQELKCPT
  PGCDGSGHVTGNYASHRSLSGCPRARKGGVKMTPTKEEKEDPELKCPVIGCDGQGHISGKYTSHRTASGC
  PLAAKRQKENPLNGASLSWKLNKQELPHCPLPGCNGLGHVNNVFVTHRSLSGCPLNAQVIKKGKVSEELM
  TIKLKATGGIESDEEIRHLDEEIKELNESNLKIEADMMKLQTQITSMESNLKTIEEENKLIEQNNESLLK
  ELAGLSQALISSLADIQLPQMGPISEQNFEAYVNTLTDMYSNLERDYSPECKALLESIKQAVKGIHV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014682
• ORF Size: 3141 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014682.2, NP_055497.1
• RefSeq Size: 6330 bp
• RefSeq ORF: 3144 bp
• Locus ID: 9705
• UniProt ID: O60284
• Cytogenetics: 8q11.23
• Domains: zf-C2HC
• Protein Families: Transcription Factors
• MW: 115.2 kDa
• Gene Summary: Repressor that binds to DNA sequences containing a bipartite element consisting of a direct repeat of the sequence 5'-AAAGTTT-3' separated by 2-9 nucleotides. Represses basal transcription activity from target promoters (By similarity). Inhibits colony formation in cultured breast cancer cells.[UniProtKB/Swiss-Prot Function]