Amylin (IAPP) (NM_000415) Human Tagged ORF Clone
IAPP (Myc-DDK-tagged)-Human islet amyloid polypeptide (IAPP)
|Product Name||Amylin (IAPP) (NM_000415) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC215074 representing NM_000415
Red=Cloning site Blue=ORF Green=Tags(s)
>RC215074 representing NM_000415
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||267 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_000415.1, NM_000415.2, NP_000406.1|
|RefSeq Size||1462 bp|
|RefSeq ORF||270 bp|
|Protein Families||Druggable Genome, Secreted Protein|
|Protein Pathways||Maturity onset diabetes of the young|
|Gene Summary||This gene encodes a member of the calcitonin family of peptide hormones. This hormone is released from pancreatic beta cells following food intake to regulate blood glucose levels and act as a satiation signal. Human patients with type 1 and advanced type 2 diabetes exhibit reduced levels of the encoded hormone in blood and pancreas. This protein also exhibits a bactericidal, antimicrobial activity. [provided by RefSeq, Jul 2016]|
|The use of this cDNA Clones has been cited in the following citations:|
IAPP-driven metabolic reprogramming induces regression of p53-deficient tumours in vivo
,Venkatanarayan, A;Raulji, P;Norton, W;Chakravarti, D;Coarfa, C;Su, X;Sandur, SK;Ramirez, MS;Lee, J;Kingsley, CV;Sananikone, EF;Rajapakshe, K;Naff, K;Parker-Thornburg, J;Bankson, JA;Tsai, KY;Gunaratne, PH;Flores, ER;,
Nature Nov 2014
,PubMed ID 25409149
|cDNA Clone Resources|
|SC300065||IAPP (untagged)-Human islet amyloid polypeptide (IAPP)||
|RG215074||IAPP (GFP-tagged) - Human islet amyloid polypeptide (IAPP)||
|RC215074L1||Lenti ORF clone of Human islet amyloid polypeptide (IAPP), Myc-DDK-tagged||
|RC215074L2||Lenti ORF clone of Human islet amyloid polypeptide (IAPP), mGFP tagged||
|RC215074L3||Lenti ORF clone of Human islet amyloid polypeptide (IAPP), Myc-DDK-tagged||
|RC215074L4||Lenti ORF clone of Human islet amyloid polypeptide (IAPP), mGFP tagged||