ILF3 (NM_012218) Human Tagged ORF Clone

CAT#: RC214999

ILF3 (Myc-DDK-tagged)-Human interleukin enhancer binding factor 3, 90kDa (ILF3), transcript variant 1

Product Images

Circular map for RC214999

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  Western blot validation of overexpression lysate (Cat# LY415895) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214999 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ILF3 protein (Cat# TP314999). The protein was produced from HEK293T cells transfected with ILF3 cDNA clone (Cat# RC214999) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ILF3
• Synonyms: CBTF; DRBF; DRBP76; MMP4; MPHOSPH4; MPP4; MPP4110; NF-AT-90; NF90; NF90a; NF90b; NF90c; NF90ctv; NF110; NF110b; NFAR; NFAR-1; NFAR-2; NFAR2; NFAR90; NFAR110; TCP80; TCP110
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214999 representing NM_012218
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGAATTTTTGTGAATGATGACCGCCATGTGATGGCAAAGCATTCTTCCGTTTATCCAACACAAGAGG
  AGCTGGAGGCAGTCCAGAACATGGTGTCCCACACGGAGCGGGCGCTCAAAGCTGTGTCCGACTGGATAGA
  CGAGCAGGAAAAGGGTAGCAGCGAGCAGGCAGAGTCCGATAACATGGATGTGCCCCCAGAGGACGACAGT
  AAAGAAGGGGCTGGGGAACAGAAGACGGAGCACATGACCAGAACCCTGCGGGGAGTGATGCGGGTGGGCC
  TGGTGGCAAAGGGCCTCCTACTCAAGGGGGACTTGGATCTGGAGCTGGTGCTGCTGTGTAAGGAGAAGCC
  CACAACCGCCCTCCTGGACAAGGTGGCCGACAACCTGGCCATCCAGCTTGCTGCTGTAACAGAAGACAAG
  TACGAAATACTGCAATCTGTCGACGATGCTGCGATTGTGATAAAAAACACAAAAGAGCCTCCATTGTCCC
  TGACCATCCACCTGACATCCCCTGTTGTCAGAGAAGAAATGGAGAAAGTATTAGCTGGAGAAACGCTATC
  AGTCAACGACCCCCCGGACGTTCTGGACAGGCAGAAATGCCTTGCTGCCTTGGCGTCCCTCCGACACGCC
  AAGTGGTTCCAGGCCAGAGCCAACGGGCTGAAGTCTTGTGTCATTGTGATCCGGGTCTTGAGGGACCTGT
  GCACTCGCGTGCCCACCTGGGGTCCCCTCCGAGGCTGGCCTCTCGAGCTCCTGTGTGAGAAATCCATTGG
  CACGGCCAACAGACCGATGGGTGCTGGCGAGGCCCTGCGGAGAGTGCTGGAGTGCCTGGCGTCGGGCATC
  GTGATGCCAGATGGTTCTGGCATTTATGACCCTTGTGAAAAAGAAGCCACTGATGCTATTGGGCATCTAG
  ACAGACAGCAACGGGAAGATATCACACAGAGTGCGCAGCACGCACTGCGGCTCGCTGCCTTCGGCCAGCT
  CCATAAAGTCCTAGGCATGGACCCTCTGCCTTCCAAGATGCCCAAGAAACCAAAGAATGAAAACCCAGTG
  GACTACACCGTTCAGATCCCACCAAGCACCACCTATGCCATTACGCCCATGAAACGCCCAATGGAGGAGG
  ACGGGGAGGAGAAGTCGCCCAGCAAAAAGAAGAAGAAGATTCAGAAGAAAGAGGAGAAGGCAGAGCCCCC
  CCAGGCTATGAATGCCCTGATGCGGTTGAACCAGCTGAAGCCAGGGCTGCAGTACAAGCTGGTGTCCCAG
  ACTGGGCCCGTCCATGCCCCCATCTTTACCATGTCTGTGGAGGTTGATGGCAATTCATTCGAGGCCTCTG
  GGCCCTCCAAAAAGACGGCCAAGCTGCACGTGGCCGTTAAGGTGTTACAGGACATGGGCTTGCCGACGGG
  TGCTGAAGGCAGGGACTCGAGCAAGGGGGAGGACTCGGCTGAGGAGACCGAGGCGAAGCCAGCAGTGGTG
  GCCCCTGCCCCAGTGGTAGAAGCTGTCTCCACCCCTAGTGCGGCCTTTCCCTCAGATGCCACTGCCGAGC
  AGGGGCCGATCCTGACAAAGCACGGCAAGAACCCAGTCATGGAGCTGAACGAGAAGAGGCGTGGGCTCAA
  GTACGAGCTCATCTCCGAGACCGGGGGCAGCCACGACAAGCGCTTCGTCATGGAGGTCGAAGTGGATGGA
  CAGAAGTTCCAAGGTGCTGGTTCCAACAAAAAGGTGGCGAAGGCCTACGCTGCTCTTGCTGCCCTAGAAA
  AGCTTTTCCCTGACACCCCTCTCGCCCTTGATGCCAACAAAAAGAAGAGAGCCCCAGTACCCGTCAGAGG
  GGGACCGAAATTTGCTGCTAAGCCACATAACCCTGGCTTCGGCATGGGAGGCCCCATGCACAACGAAGTG
  CCCCCACCCCCCAACCTTCGAGGGCGGGGAAGAGGCGGGAGCATCCGGGGACGAGGGCGCGGGCGAGGAT
  TTGGTGGCGCCAACCATGGAGGCTACATGAATGCCGGTGCTGGGTATGGAAGCTATGGGTACGGAGGCAA
  CTCGGCGACAGCAGGCTACAGTCAGTTCTACAGCAACGGAGGGCATTCTGGGAATGCCAGTGGCGGTGGC
  GGCGGGGGCGGTGGTGGCTCCTCCGGCTATGGCTCCTACTACCAAGGTGACAACTACAACTCACCGGTGC
  CCCCAAAACACGCTGGGAAGAAGCAGCCGCACGGGGGCCAGCAGAAGCCCTCCTACGGCTCGGGCTACCA
  GTCCCACCAGGGCCAGCAGCAGTCCTACAACCAGAGCCCCTACAGCAACTATGGCCCTCCACAGGGCAAG
  CAGAAAGGCTATAACCATGGACAAGGCAGCTACTCCTACTCGAACTCCTACAACTCTCCCGGGGGCGGGG
  GCGGATCCGACTACAACTACGAGAGCAAATTCAACTACAGTGGTAGTGGAGGCCGAAGCGGCGGGAACAG
  CTACGGCTCAGGCGGGGCATCCTACAACCCAGGGTCACACGGGGGCTACGGCGGAGGTTCTGGGGGCGGC
  TCCTCATACCAAGGCAAACAAGGAGGCTACTCACAGTCGAACTACAACTCCCCGGGGTCCGGCCAGAACT
  ACAGTGGCCCTCCCAGCTCCTACCAGTCCTCACAAGGCGGCTATGGCAGAAACGCAGACCACAGCATGAA
  CTACCAGTACAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214999 representing NM_012218
  Red=Cloning site Green=Tags(s)
  
  MRIFVNDDRHVMAKHSSVYPTQEELEAVQNMVSHTERALKAVSDWIDEQEKGSSEQAESDNMDVPPEDDS
  KEGAGEQKTEHMTRTLRGVMRVGLVAKGLLLKGDLDLELVLLCKEKPTTALLDKVADNLAIQLAAVTEDK
  YEILQSVDDAAIVIKNTKEPPLSLTIHLTSPVVREEMEKVLAGETLSVNDPPDVLDRQKCLAALASLRHA
  KWFQARANGLKSCVIVIRVLRDLCTRVPTWGPLRGWPLELLCEKSIGTANRPMGAGEALRRVLECLASGI
  VMPDGSGIYDPCEKEATDAIGHLDRQQREDITQSAQHALRLAAFGQLHKVLGMDPLPSKMPKKPKNENPV
  DYTVQIPPSTTYAITPMKRPMEEDGEEKSPSKKKKKIQKKEEKAEPPQAMNALMRLNQLKPGLQYKLVSQ
  TGPVHAPIFTMSVEVDGNSFEASGPSKKTAKLHVAVKVLQDMGLPTGAEGRDSSKGEDSAEETEAKPAVV
  APAPVVEAVSTPSAAFPSDATAEQGPILTKHGKNPVMELNEKRRGLKYELISETGGSHDKRFVMEVEVDG
  QKFQGAGSNKKVAKAYAALAALEKLFPDTPLALDANKKKRAPVPVRGGPKFAAKPHNPGFGMGGPMHNEV
  PPPPNLRGRGRGGSIRGRGRGRGFGGANHGGYMNAGAGYGSYGYGGNSATAGYSQFYSNGGHSGNASGGG
  GGGGGGSSGYGSYYQGDNYNSPVPPKHAGKKQPHGGQQKPSYGSGYQSHQGQQQSYNQSPYSNYGPPQGK
  QKGYNHGQGSYSYSNSYNSPGGGGGSDYNYESKFNYSGSGGRSGGNSYGSGGASYNPGSHGGYGGGSGGG
  SSYQGKQGGYSQSNYNSPGSGQNYSGPPSSYQSSQGGYGRNADHSMNYQYR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012218
• ORF Size: 2673 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012218.4
• RefSeq Size: 6058 bp
• RefSeq ORF: 2685 bp
• Locus ID: 3609
• UniProt ID: Q12906
• Cytogenetics: 19p13.2
• Domains: DSRM
• Protein Families: Druggable Genome, Transcription Factors
• MW: 95.2 kDa
• Gene Summary: This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein (NF90, ILF3) forms a heterodimer with a 45 kDa transcription factor (NF45, ILF2) required for T-cell expression of interleukin 2. This complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2014]