PERK (EIF2AK3) (NM_004836) Human Tagged ORF Clone

CAT#: RC214993

EIF2AK3 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3)

Product Images

Western blot validation of overexpression lysate (Cat# LY417724) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214993 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified EIF2AK3 protein (Cat# TP314993). The protein was produced from HEK293T cells transfected with EIF2AK3 cDNA clone (Cat# RC214993) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PERK
• Synonyms: PEK; PERK; WRS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214993 representing NM_004836
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGCGCCATCAGCCCGGGGCTGCTGGTACGGGCGCTGCTGCTGCTGCTGCTGCTGCTGGGGCTCG
  CGGCAAGGACGGTGGCCGCGGGGCGCGCCCGTGGCCTCCCAGCGCCGACGGCGGAGGCGGCGTTCGGCCT
  CGGGGCGGCCGCTGCTCCCACCTCAGCGACGCGAGTACCGGCGGCGGGCGCCGTGGCTGCGGCCGAGGTG
  ACTGTGGAGGACGCTGAGGCGCTGCCGGCAGCCGCGGGAGAGCAGGAGCCTCGGGGTCCGGAACCAGACG
  ATGAGACAGAGTTGCGACCGCGCGGCAGGTCATTAGTAATTATCAGCACTTTAGATGGGAGAATTGCTGC
  CTTGGATCCTGAAAATCATGGTAAAAAGCAGTGGGATTTGGATGTGGGATCCGGTTCCTTGGTGTCATCC
  AGCCTTAGCAAACCAGAGGTATTTGGGAATAAGATGATCATTCCTTCCCTGGATGGAGCCCTCTTCCAGT
  GGGACCGAGACCGTGAAAGCATGGAAACAGTTCCTTTCACAGTTGAATCACTTCTTGAATCTTCTTATAA
  ATTTGGAGATGATGTTGTTTTGGTTGGAGGAAAATCTCTGACTACATATGGACTCAGTGCATATAGTGGA
  AAGGTGAGGTATATCTGTTCAGCTCTGGGTTGTCGCCAATGGGATAGTGACGAAATGGAACAAGAGGAAG
  ACATCCTGCTTCTACAGCGTACCCAAAAAACTGTTAGAGCTGTCGGACCTCGCAGTGGCAATGAGAAGTG
  GAATTTCAGTGTTGGCCACTTTGAACTTCGGTATATTCCAGACATGGAAACGAGAGCCGGATTTATTGAA
  AGCACCTTTAAGCCCAATGAGAACACAGAAGAGTCTAAAATTATTTCAGATGTGGAAGAACAGGAAGCTG
  CCATAATGGACATAGTGATAAAGGTTTCGGTTGCTGACTGGAAAGTTATGGCATTCAGTAAGAAGGGAGG
  ACATCTGGAATGGGAGTACCAGTTTTGTACTCCAATTGCATCTGCCTGGTTACTTAAGGATGGGAAAGTC
  ATTCCCATCAGTCTTTTTGATGATACAAGTTATACATCTAATGATGATGTTTTAGAAGATGAAGAAGACA
  TTGTAGAAGCTGCCAGAGGAGCCACAGAAAACAGTGTTTACTTGGGAATGTATAGAGGCCAGCTGTATCT
  GCAGTCATCAGTCAGAATTTCAGAAAAGTTTCCTTCAAGTCCCAAGGCTTTGGAATCTGTCACTAATGAA
  AACGCAATTATTCCTTTACCAACAATCAAATGGAAACCCTTAATTCATTCTCCTTCCAGAACTCCTGTCT
  TGGTAGGATCTGATGAATTTGACAAATGTCTCAGTAATGATAAGTTTTCTCATGAAGAATATAGTAATGG
  TGCACTTTCAATCTTGCAGTATCCATATGATAATGGTTATTATCTACCATACTACAAGAGGGAGAGGAAC
  AAACGAAGCACACAGATTACAGTCAGATTCCTCGACAACCCACATTACAACAAGAATATCCGCAAAAAGG
  ATCCTGTTCTTCTTTTACACTGGTGGAAAGAAATAGTTGCAACGATTTTGTTTTGTATCATAGCAACAAC
  GTTTATTGTGCGCAGGCTTTTCCATCCTCATCCTCACAGGCAAAGGAAGGAGTCTGAAACTCAGTGTCAA
  ACTGAAAATAAATATGATTCTGTAAGTGGTGAAGCCAATGACAGTAGCTGGAATGACATAAAAAACTCTG
  GATATATATCACGATATCTAACTGATTTTGAGCCAATTCAATGCCTGGGACGTGGTGGCTTTGGAGTTGT
  TTTTGAAGCTAAAAACAAAGTAGATGACTGCAATTATGCTATCAAGAGGATCCGTCTCCCCAATAGGGAA
  TTGGCTCGGGAAAAGGTAATGCGAGAAGTTAAAGCCTTAGCCAAGCTTGAACACCCGGGCATTGTTAGAT
  ATTTCAATGCCTGGCTCGAAGCACCACCAGAGAAGTGGCAAGAAAAGATGGATGAAATTTGGCTGAAAGA
  TGAAAGCACAGACTGGCCACTCAGCTCTCCTAGCCCAATGGATGCACCATCAGTTAAAATACGCAGAATG
  GATCCTTTCTCTACAAAAGAACATATTGAAATCATAGCTCCTTCACCACAAAGAAGCAGGTCTTTTTCAG
  TAGGGATTTCCTGTGACCAGACAAGTTCATCTGAGAGCCAGTTCTCACCACTGGAATTCTCAGGAATGGA
  CCATGAGGACATCAGTGAGTCAGTGGATGCAGCATACAACCTCCAGGACAGTTGCCTTACAGACTGTGAT
  GTGGAAGATGGGACTATGGATGGCAATGATGAGGGGCACTCCTTTGAACTTTGTCCTTCTGAAGCTTCTC
  CTTATGTAAGGTCAAGGGAGAGAACCTCCTCTTCAATAGTATTTGAAGATTCTGGCTGTGATAATGCTTC
  CAGTAAAGAAGAGCCGAAAACTAATCGATTGCATATTGGCAACCATTGTGCTAATAAACTAACTGCTTTC
  AAGCCCACCAGTAGCAAATCTTCTTCTGAAGCTACATTGTCTATTTCTCCTCCAAGACCAACCACTTTAA
  GTTTAGATCTCACTAAAAACACCACAGAAAAACTCCAGCCCAGTTCACCAAAGGTGTATCTTTACATTCA
  AATGCAGCTGTGCAGAAAAGAAAACCTCAAAGACTGGATGAATGGACGATGTACCATAGAGGAGAGAGAG
  AGGAGCGTGTGTCTGCACATCTTCCTGCAGATCGCAGAGGCAGTGGAGTTTCTTCACAGTAAAGGACTGA
  TGCACAGGGACCTCAAGCCATCCAACATATTCTTTACAATGGATGATGTGGTCAAGGTTGGAGACTTTGG
  GTTAGTGACTGCAATGGACCAGGATGAGGAAGAGCAGACGGTTCTGACCCCAATGCCAGCTTATGCCAGA
  CACACAGGACAAGTAGGGACCAAACTGTATATGAGCCCAGAGCAGATTCATGGAAACAGCTATTCTCATA
  AAGTGGACATCTTTTCTTTAGGCCTGATTCTATTTGAATTGCTGTATCCATTCAGCACTCAGATGGAGAG
  AGTCAGGACCTTAACTGATGTAAGAAATCTCAAATTTCCACCATTATTTACTCAGAAATATCCTTGTGAG
  TACGTGATGGTTCAAGACATGCTCTCTCCATCCCCCATGGAACGACCTGAAGCTATAAACATCATTGAAA
  ATGCTGTATTTGAGGACTTGGACTTTCCAGGAAAAACAGTGCTCAGACAGAGGTCTCGCTCCTTGAGTTC
  ATCGGGAACAAAACATTCAAGACAGTCCAACAACTCCCATAGCCCTTTGCCAAGCAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214993 representing NM_004836
  Red=Cloning site Green=Tags(s)
  
  MERAISPGLLVRALLLLLLLLGLAARTVAAGRARGLPAPTAEAAFGLGAAAAPTSATRVPAAGAVAAAEV
  TVEDAEALPAAAGEQEPRGPEPDDETELRPRGRSLVIISTLDGRIAALDPENHGKKQWDLDVGSGSLVSS
  SLSKPEVFGNKMIIPSLDGALFQWDRDRESMETVPFTVESLLESSYKFGDDVVLVGGKSLTTYGLSAYSG
  KVRYICSALGCRQWDSDEMEQEEDILLLQRTQKTVRAVGPRSGNEKWNFSVGHFELRYIPDMETRAGFIE
  STFKPNENTEESKIISDVEEQEAAIMDIVIKVSVADWKVMAFSKKGGHLEWEYQFCTPIASAWLLKDGKV
  IPISLFDDTSYTSNDDVLEDEEDIVEAARGATENSVYLGMYRGQLYLQSSVRISEKFPSSPKALESVTNE
  NAIIPLPTIKWKPLIHSPSRTPVLVGSDEFDKCLSNDKFSHEEYSNGALSILQYPYDNGYYLPYYKRERN
  KRSTQITVRFLDNPHYNKNIRKKDPVLLLHWWKEIVATILFCIIATTFIVRRLFHPHPHRQRKESETQCQ
  TENKYDSVSGEANDSSWNDIKNSGYISRYLTDFEPIQCLGRGGFGVVFEAKNKVDDCNYAIKRIRLPNRE
  LAREKVMREVKALAKLEHPGIVRYFNAWLEAPPEKWQEKMDEIWLKDESTDWPLSSPSPMDAPSVKIRRM
  DPFSTKEHIEIIAPSPQRSRSFSVGISCDQTSSSESQFSPLEFSGMDHEDISESVDAAYNLQDSCLTDCD
  VEDGTMDGNDEGHSFELCPSEASPYVRSRERTSSSIVFEDSGCDNASSKEEPKTNRLHIGNHCANKLTAF
  KPTSSKSSSEATLSISPPRPTTLSLDLTKNTTEKLQPSSPKVYLYIQMQLCRKENLKDWMNGRCTIEERE
  RSVCLHIFLQIAEAVEFLHSKGLMHRDLKPSNIFFTMDDVVKVGDFGLVTAMDQDEEEQTVLTPMPAYAR
  HTGQVGTKLYMSPEQIHGNSYSHKVDIFSLGLILFELLYPFSTQMERVRTLTDVRNLKFPPLFTQKYPCE
  YVMVQDMLSPSPMERPEAINIIENAVFEDLDFPGKTVLRQRSRSLSSSGTKHSRQSNNSHSPLPSN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004836
• ORF Size: 3348 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004836.7
• RefSeq Size: 4511 bp
• RefSeq ORF: 3351 bp
• Locus ID: 9451
• UniProt ID: Q9NZJ5
• Cytogenetics: 2p11.2
• Domains: pkinase, TyrKc, S_TKc, PQQ
• Protein Families: Druggable Genome, Protein Kinase, Secreted Protein, Transmembrane
• Protein Pathways: Alzheimer's disease
• MW: 125.26 kDa
• Gene Summary: The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]