PERK (EIF2AK3) (NM_004836) Human Tagged ORF Clone

CAT#: RC214993

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EIF2AK3 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3)



  "NM_004836" in other vectors (7)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 1,326.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol EIF2AK3
Synonyms PEK; PERK; WRS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC214993 representing NM_004836
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCGCGCCATCAGCCCGGGGCTGCTGGTACGGGCGCTGCTGCTGCTGCTGCTGCTGCTGGGGCTCG
CGGCAAGGACGGTGGCCGCGGGGCGCGCCCGTGGCCTCCCAGCGCCGACGGCGGAGGCGGCGTTCGGCCT
CGGGGCGGCCGCTGCTCCCACCTCAGCGACGCGAGTACCGGCGGCGGGCGCCGTGGCTGCGGCCGAGGTG
ACTGTGGAGGACGCTGAGGCGCTGCCGGCAGCCGCGGGAGAGCAGGAGCCTCGGGGTCCGGAACCAGACG
ATGAGACAGAGTTGCGACCGCGCGGCAGGTCATTAGTAATTATCAGCACTTTAGATGGGAGAATTGCTGC
CTTGGATCCTGAAAATCATGGTAAAAAGCAGTGGGATTTGGATGTGGGATCCGGTTCCTTGGTGTCATCC
AGCCTTAGCAAACCAGAGGTATTTGGGAATAAGATGATCATTCCTTCCCTGGATGGAGCCCTCTTCCAGT
GGGACCGAGACCGTGAAAGCATGGAAACAGTTCCTTTCACAGTTGAATCACTTCTTGAATCTTCTTATAA
ATTTGGAGATGATGTTGTTTTGGTTGGAGGAAAATCTCTGACTACATATGGACTCAGTGCATATAGTGGA
AAGGTGAGGTATATCTGTTCAGCTCTGGGTTGTCGCCAATGGGATAGTGACGAAATGGAACAAGAGGAAG
ACATCCTGCTTCTACAGCGTACCCAAAAAACTGTTAGAGCTGTCGGACCTCGCAGTGGCAATGAGAAGTG
GAATTTCAGTGTTGGCCACTTTGAACTTCGGTATATTCCAGACATGGAAACGAGAGCCGGATTTATTGAA
AGCACCTTTAAGCCCAATGAGAACACAGAAGAGTCTAAAATTATTTCAGATGTGGAAGAACAGGAAGCTG
CCATAATGGACATAGTGATAAAGGTTTCGGTTGCTGACTGGAAAGTTATGGCATTCAGTAAGAAGGGAGG
ACATCTGGAATGGGAGTACCAGTTTTGTACTCCAATTGCATCTGCCTGGTTACTTAAGGATGGGAAAGTC
ATTCCCATCAGTCTTTTTGATGATACAAGTTATACATCTAATGATGATGTTTTAGAAGATGAAGAAGACA
TTGTAGAAGCTGCCAGAGGAGCCACAGAAAACAGTGTTTACTTGGGAATGTATAGAGGCCAGCTGTATCT
GCAGTCATCAGTCAGAATTTCAGAAAAGTTTCCTTCAAGTCCCAAGGCTTTGGAATCTGTCACTAATGAA
AACGCAATTATTCCTTTACCAACAATCAAATGGAAACCCTTAATTCATTCTCCTTCCAGAACTCCTGTCT
TGGTAGGATCTGATGAATTTGACAAATGTCTCAGTAATGATAAGTTTTCTCATGAAGAATATAGTAATGG
TGCACTTTCAATCTTGCAGTATCCATATGATAATGGTTATTATCTACCATACTACAAGAGGGAGAGGAAC
AAACGAAGCACACAGATTACAGTCAGATTCCTCGACAACCCACATTACAACAAGAATATCCGCAAAAAGG
ATCCTGTTCTTCTTTTACACTGGTGGAAAGAAATAGTTGCAACGATTTTGTTTTGTATCATAGCAACAAC
GTTTATTGTGCGCAGGCTTTTCCATCCTCATCCTCACAGGCAAAGGAAGGAGTCTGAAACTCAGTGTCAA
ACTGAAAATAAATATGATTCTGTAAGTGGTGAAGCCAATGACAGTAGCTGGAATGACATAAAAAACTCTG
GATATATATCACGATATCTAACTGATTTTGAGCCAATTCAATGCCTGGGACGTGGTGGCTTTGGAGTTGT
TTTTGAAGCTAAAAACAAAGTAGATGACTGCAATTATGCTATCAAGAGGATCCGTCTCCCCAATAGGGAA
TTGGCTCGGGAAAAGGTAATGCGAGAAGTTAAAGCCTTAGCCAAGCTTGAACACCCGGGCATTGTTAGAT
ATTTCAATGCCTGGCTCGAAGCACCACCAGAGAAGTGGCAAGAAAAGATGGATGAAATTTGGCTGAAAGA
TGAAAGCACAGACTGGCCACTCAGCTCTCCTAGCCCAATGGATGCACCATCAGTTAAAATACGCAGAATG
GATCCTTTCTCTACAAAAGAACATATTGAAATCATAGCTCCTTCACCACAAAGAAGCAGGTCTTTTTCAG
TAGGGATTTCCTGTGACCAGACAAGTTCATCTGAGAGCCAGTTCTCACCACTGGAATTCTCAGGAATGGA
CCATGAGGACATCAGTGAGTCAGTGGATGCAGCATACAACCTCCAGGACAGTTGCCTTACAGACTGTGAT
GTGGAAGATGGGACTATGGATGGCAATGATGAGGGGCACTCCTTTGAACTTTGTCCTTCTGAAGCTTCTC
CTTATGTAAGGTCAAGGGAGAGAACCTCCTCTTCAATAGTATTTGAAGATTCTGGCTGTGATAATGCTTC
CAGTAAAGAAGAGCCGAAAACTAATCGATTGCATATTGGCAACCATTGTGCTAATAAACTAACTGCTTTC
AAGCCCACCAGTAGCAAATCTTCTTCTGAAGCTACATTGTCTATTTCTCCTCCAAGACCAACCACTTTAA
GTTTAGATCTCACTAAAAACACCACAGAAAAACTCCAGCCCAGTTCACCAAAGGTGTATCTTTACATTCA
AATGCAGCTGTGCAGAAAAGAAAACCTCAAAGACTGGATGAATGGACGATGTACCATAGAGGAGAGAGAG
AGGAGCGTGTGTCTGCACATCTTCCTGCAGATCGCAGAGGCAGTGGAGTTTCTTCACAGTAAAGGACTGA
TGCACAGGGACCTCAAGCCATCCAACATATTCTTTACAATGGATGATGTGGTCAAGGTTGGAGACTTTGG
GTTAGTGACTGCAATGGACCAGGATGAGGAAGAGCAGACGGTTCTGACCCCAATGCCAGCTTATGCCAGA
CACACAGGACAAGTAGGGACCAAACTGTATATGAGCCCAGAGCAGATTCATGGAAACAGCTATTCTCATA
AAGTGGACATCTTTTCTTTAGGCCTGATTCTATTTGAATTGCTGTATCCATTCAGCACTCAGATGGAGAG
AGTCAGGACCTTAACTGATGTAAGAAATCTCAAATTTCCACCATTATTTACTCAGAAATATCCTTGTGAG
TACGTGATGGTTCAAGACATGCTCTCTCCATCCCCCATGGAACGACCTGAAGCTATAAACATCATTGAAA
ATGCTGTATTTGAGGACTTGGACTTTCCAGGAAAAACAGTGCTCAGACAGAGGTCTCGCTCCTTGAGTTC
ATCGGGAACAAAACATTCAAGACAGTCCAACAACTCCCATAGCCCTTTGCCAAGCAAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC214993 representing NM_004836
Red=Cloning site Green=Tags(s)

MERAISPGLLVRALLLLLLLLGLAARTVAAGRARGLPAPTAEAAFGLGAAAAPTSATRVPAAGAVAAAEV
TVEDAEALPAAAGEQEPRGPEPDDETELRPRGRSLVIISTLDGRIAALDPENHGKKQWDLDVGSGSLVSS
SLSKPEVFGNKMIIPSLDGALFQWDRDRESMETVPFTVESLLESSYKFGDDVVLVGGKSLTTYGLSAYSG
KVRYICSALGCRQWDSDEMEQEEDILLLQRTQKTVRAVGPRSGNEKWNFSVGHFELRYIPDMETRAGFIE
STFKPNENTEESKIISDVEEQEAAIMDIVIKVSVADWKVMAFSKKGGHLEWEYQFCTPIASAWLLKDGKV
IPISLFDDTSYTSNDDVLEDEEDIVEAARGATENSVYLGMYRGQLYLQSSVRISEKFPSSPKALESVTNE
NAIIPLPTIKWKPLIHSPSRTPVLVGSDEFDKCLSNDKFSHEEYSNGALSILQYPYDNGYYLPYYKRERN
KRSTQITVRFLDNPHYNKNIRKKDPVLLLHWWKEIVATILFCIIATTFIVRRLFHPHPHRQRKESETQCQ
TENKYDSVSGEANDSSWNDIKNSGYISRYLTDFEPIQCLGRGGFGVVFEAKNKVDDCNYAIKRIRLPNRE
LAREKVMREVKALAKLEHPGIVRYFNAWLEAPPEKWQEKMDEIWLKDESTDWPLSSPSPMDAPSVKIRRM
DPFSTKEHIEIIAPSPQRSRSFSVGISCDQTSSSESQFSPLEFSGMDHEDISESVDAAYNLQDSCLTDCD
VEDGTMDGNDEGHSFELCPSEASPYVRSRERTSSSIVFEDSGCDNASSKEEPKTNRLHIGNHCANKLTAF
KPTSSKSSSEATLSISPPRPTTLSLDLTKNTTEKLQPSSPKVYLYIQMQLCRKENLKDWMNGRCTIEERE
RSVCLHIFLQIAEAVEFLHSKGLMHRDLKPSNIFFTMDDVVKVGDFGLVTAMDQDEEEQTVLTPMPAYAR
HTGQVGTKLYMSPEQIHGNSYSHKVDIFSLGLILFELLYPFSTQMERVRTLTDVRNLKFPPLFTQKYPCE
YVMVQDMLSPSPMERPEAINIIENAVFEDLDFPGKTVLRQRSRSLSSSGTKHSRQSNNSHSPLPSN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004836
ORF Size 3348 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004836.7
RefSeq Size 4511 bp
RefSeq ORF 3351 bp
Locus ID 9451
UniProt ID Q9NZJ5
Cytogenetics 2p11.2
Domains pkinase, TyrKc, S_TKc, PQQ
Protein Families Druggable Genome, Protein Kinase, Secreted Protein, Transmembrane
Protein Pathways Alzheimer's disease
MW 125.26 kDa
Gene Summary The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]

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