MYLK4 (NM_001012418) Human Tagged ORF Clone

CAT#: RC214937

MYLK4 (Myc-DDK-tagged)-Human myosin light chain kinase family, member 4 (MYLK4)

Product Images

Circular map for RC214937

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  Western blot validation of overexpression lysate (Cat# LY422856) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214937 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MYLK4 protein (Cat# TP314937). The protein was produced from HEK293T cells transfected with MYLK4 cDNA clone (Cat# RC214937) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MYLK4
• Synonyms: SgK085
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214937 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTAAAAGTGAAGAGGCTGGAAGAATTCAACACGTGTTATAACAGCAACCAGCTGGAGAAAATGGCCT
  TTTTTCAGTGCAGGGAAGAGGTGGAGAAAGTGAAGTGTTTTCTGGAAAAAAATTCTGGGGACCAGGATTC
  AAGATCTAGACATAATGAGGCGAAGGAGGTGTGGTCAAACGCCGACCTGACGGAAAGGATGCCCGTCAAA
  AGCAAAAGGACATCAGCCCTCGCAGTTGACATCCCGGCTCCTCCGGCCCCATTTGATCATCGTATTGTGA
  CAGCCAAGCAAGGAGCGGTCAACAGCTTCTATACTGTGAGCAAGACAGAAATCCTAGGAGGAGGGCGTTT
  CGGCCAGGTTCACAAGTGTGAGGAGACGGCCACAGGTCTGAAGCTGGCAGCCAAAATCATCAAGACCAGA
  GGCATGAAGGACAAGGAGGAGGTGAAGAACGAGATCAGCGTCATGAACCAGCTGGACCACGCGAACCTCA
  TCCAGCTGTACGATGCCTTCGAGTCTAAGAACGACATTGTCCTGGTCATGGAGTATGTGGATGGTGGGGA
  GCTGTTTGACCGCATCATCGATGAGAGCTACAATTTGACGGAGCTTGATACCATCCTGTTCATGAAGCAG
  ATATGTGAGGGGATAAGGCACATGCATCAGATGTACATTCTCCACTTGGACCTGAAGCCTGAGAATATCC
  TGTGTGTGAATCGGGATGCTAAGCAAATAAAAATTATTGATTTTGGATTGGCCAGAAGATACAAACCCAG
  AGAGAAGCTGAAGGTGAACTTTGGAACCCCAGAATTTCTCGCCCCTGAAGTTGTGAACTATGATTTTGTT
  TCATTTCCCACTGACATGTGGAGTGTGGGGGTCATCGCCTATATGCTACTTAGCGGTTTGTCGCCTTTCC
  TGGGTGACAATGATGCTGAGACGCTGAACAACATCCTGGCCTGCAGGTGGGACTTAGAGGATGAAGAATT
  TCAGGACATCTCGGAGGAGGCCAAGGAGTTCATCTCTAAGCTTCTGATTAAGGAGAAGAGTTGGCGAATA
  AGTGCAAGCGAAGCTCTCAAGCACCCCTGGTTGTCAGACCACAAGCTCCACTCCAGACTCAATGCCCAGA
  AGAAGAAGAATCGTGGCTCTGATGCCCAGGACTTTGTGACCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214937 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLKVKRLEEFNTCYNSNQLEKMAFFQCREEVEKVKCFLEKNSGDQDSRSRHNEAKEVWSNADLTERMPVK
  SKRTSALAVDIPAPPAPFDHRIVTAKQGAVNSFYTVSKTEILGGGRFGQVHKCEETATGLKLAAKIIKTR
  GMKDKEEVKNEISVMNQLDHANLIQLYDAFESKNDIVLVMEYVDGGELFDRIIDESYNLTELDTILFMKQ
  ICEGIRHMHQMYILHLDLKPENILCVNRDAKQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYDFV
  SFPTDMWSVGVIAYMLLSGLSPFLGDNDAETLNNILACRWDLEDEEFQDISEEAKEFISKLLIKEKSWRI
  SASEALKHPWLSDHKLHSRLNAQKKKNRGSDAQDFVTK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012418
• ORF Size: 1164 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012418.5
• RefSeq Size: 5760 bp
• RefSeq ORF: 1167 bp
• Locus ID: 340156
• UniProt ID: Q86YV6
• Cytogenetics: 6p25.2
• Protein Families: Druggable Genome, Protein Kinase
• MW: 44.6 kDa