MYLK4 (NM_001012418) Human Tagged ORF Clone

CAT#: RC214937

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MYLK4 (Myc-DDK-tagged)-Human myosin light chain kinase family, member 4 (MYLK4)



  "NM_001012418" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol MYLK4
Synonyms SgK085
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC214937 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTAAAAGTGAAGAGGCTGGAAGAATTCAACACGTGTTATAACAGCAACCAGCTGGAGAAAATGGCCT
TTTTTCAGTGCAGGGAAGAGGTGGAGAAAGTGAAGTGTTTTCTGGAAAAAAATTCTGGGGACCAGGATTC
AAGATCTAGACATAATGAGGCGAAGGAGGTGTGGTCAAACGCCGACCTGACGGAAAGGATGCCCGTCAAA
AGCAAAAGGACATCAGCCCTCGCAGTTGACATCCCGGCTCCTCCGGCCCCATTTGATCATCGTATTGTGA
CAGCCAAGCAAGGAGCGGTCAACAGCTTCTATACTGTGAGCAAGACAGAAATCCTAGGAGGAGGGCGTTT
CGGCCAGGTTCACAAGTGTGAGGAGACGGCCACAGGTCTGAAGCTGGCAGCCAAAATCATCAAGACCAGA
GGCATGAAGGACAAGGAGGAGGTGAAGAACGAGATCAGCGTCATGAACCAGCTGGACCACGCGAACCTCA
TCCAGCTGTACGATGCCTTCGAGTCTAAGAACGACATTGTCCTGGTCATGGAGTATGTGGATGGTGGGGA
GCTGTTTGACCGCATCATCGATGAGAGCTACAATTTGACGGAGCTTGATACCATCCTGTTCATGAAGCAG
ATATGTGAGGGGATAAGGCACATGCATCAGATGTACATTCTCCACTTGGACCTGAAGCCTGAGAATATCC
TGTGTGTGAATCGGGATGCTAAGCAAATAAAAATTATTGATTTTGGATTGGCCAGAAGATACAAACCCAG
AGAGAAGCTGAAGGTGAACTTTGGAACCCCAGAATTTCTCGCCCCTGAAGTTGTGAACTATGATTTTGTT
TCATTTCCCACTGACATGTGGAGTGTGGGGGTCATCGCCTATATGCTACTTAGCGGTTTGTCGCCTTTCC
TGGGTGACAATGATGCTGAGACGCTGAACAACATCCTGGCCTGCAGGTGGGACTTAGAGGATGAAGAATT
TCAGGACATCTCGGAGGAGGCCAAGGAGTTCATCTCTAAGCTTCTGATTAAGGAGAAGAGTTGGCGAATA
AGTGCAAGCGAAGCTCTCAAGCACCCCTGGTTGTCAGACCACAAGCTCCACTCCAGACTCAATGCCCAGA
AGAAGAAGAATCGTGGCTCTGATGCCCAGGACTTTGTGACCAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC214937 protein sequence
Red=Cloning site Green=Tags(s)

MLKVKRLEEFNTCYNSNQLEKMAFFQCREEVEKVKCFLEKNSGDQDSRSRHNEAKEVWSNADLTERMPVK
SKRTSALAVDIPAPPAPFDHRIVTAKQGAVNSFYTVSKTEILGGGRFGQVHKCEETATGLKLAAKIIKTR
GMKDKEEVKNEISVMNQLDHANLIQLYDAFESKNDIVLVMEYVDGGELFDRIIDESYNLTELDTILFMKQ
ICEGIRHMHQMYILHLDLKPENILCVNRDAKQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYDFV
SFPTDMWSVGVIAYMLLSGLSPFLGDNDAETLNNILACRWDLEDEEFQDISEEAKEFISKLLIKEKSWRI
SASEALKHPWLSDHKLHSRLNAQKKKNRGSDAQDFVTK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001012418
ORF Size 1164 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001012418.5
RefSeq Size 5760 bp
RefSeq ORF 1167 bp
Locus ID 340156
UniProt ID Q86YV6
Cytogenetics 6p25.2
Protein Families Druggable Genome, Protein Kinase
MW 44.6 kDa

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