L Kynurenine Hydrolase (KYNU) (NM_003937) Human Tagged ORF Clone

CAT#: RC214932

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KYNU (Myc-DDK-tagged)-Human kynureninase (KYNU), transcript variant 1



  "NM_003937" in other vectors (6)

Reconstitution Protocol

USD 686.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol KYNU
Synonyms KYNUU; VCRL2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC214932 representing NM_003937
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCTTCATCTCTTGAGCTGCCGGCTGACACAGTGCAGCGCATTGCGGCTGAACTCAAATGCCACC
CAACGGATGAGAGGGTGGCTCTCCACCTAGATGAGGAAGATAAGCTGAGGCACTTCAGGGAGTGCTTTTA
TATTCCCAAAATACAGGATCTGCCTCCAGTTGATTTATCATTAGTGAATAAAGATGAAAATGCCATCTAT
TTCTTGGGAAATTCTCTTGGCCTTCAACCAAAAATGGTTAAAACATATCTTGAAGAAGAACTAGATAAGT
GGGCCAAAATAGCAGCCTATGGTCATGAAGTGGGGAAGCGTCCTTGGATTACAGGAGATGAGAGTATTGT
AGGCCTTATGAAGGACATTGTAGGAGCCAATGAGAAAGAAATAGCCCTAATGAATGCTTTGACTGTAAAT
TTACATCTTCTAATGTTATCATTTTTTAAGCCTACGCCAAAACGATATAAAATTCTTCTAGAAGCCAAAG
CCTTCCCTTCTGATCATTATGCTATTGAGTCACAACTACAACTTCACGGACTTAACATTGAAGAAAGTAT
GCGGATGATAAAGCCAAGAGAGGGGGAAGAAACCTTAAGAATAGAGGATATCCTTGAAGTAATTGAGAAG
GAAGGAGACTCAATTGCAGTGATCCTGTTCAGTGGGGTGCATTTTTACACTGGACAGCACTTTAATATTC
CTGCCATCACAAAAGCTGGACAAGCGAAGGGTTGTTATGTTGGCTTTGATCTAGCACATGCAGTTGGAAA
TGTTGAACTCTACTTACATGACTGGGGAGTTGATTTTGCCTGCTGGTGTTCCTACAAGTATTTAAATGCA
GGAGCAGGAGGAATTGCTGGTGCCTTCATTCATGAAAAGCATGCCCATACGATTAAACCTGCATTAGTGG
GATGGTTTGGCCATGAACTCAGCACCAGATTTAAGATGGATAACAAACTGCAGTTAATCCCTGGGGTCTG
TGGATTCCGAATTTCAAATCCTCCCATTTTGTTGGTCTGTTCCTTGCATGCTAGTTTAGAGATCTTTAAG
CAAGCGACAATGAAGGCATTGCGGAAAAAATCTGTTTTGCTAACTGGCTATCTGGAATACCTGATCAAGC
ATAACTATGGCAAAGATAAAGCAGCAACCAAGAAACCAGTTGTGAACATAATTACTCCGTCTCATGTAGA
GGAGCGGGGGTGCCAGCTAACAATAACATTTTCTGTTCCAAACAAAGATGTTTTCCAAGAACTAGAAAAA
AGAGGAGTGGTTTGTGACAAGCGGAATCCAAATGGCATTCGAGTGGCTCCAGTTCCTCTCTATAATTCTT
TCCATGATGTTTATAAATTTACCAATCTGCTCACTTCTATACTTGACTCTGCAGAAACAAAAAAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC214932 representing NM_003937
Red=Cloning site Green=Tags(s)

MEPSSLELPADTVQRIAAELKCHPTDERVALHLDEEDKLRHFRECFYIPKIQDLPPVDLSLVNKDENAIY
FLGNSLGLQPKMVKTYLEEELDKWAKIAAYGHEVGKRPWITGDESIVGLMKDIVGANEKEIALMNALTVN
LHLLMLSFFKPTPKRYKILLEAKAFPSDHYAIESQLQLHGLNIEESMRMIKPREGEETLRIEDILEVIEK
EGDSIAVILFSGVHFYTGQHFNIPAITKAGQAKGCYVGFDLAHAVGNVELYLHDWGVDFACWCSYKYLNA
GAGGIAGAFIHEKHAHTIKPALVGWFGHELSTRFKMDNKLQLIPGVCGFRISNPPILLVCSLHASLEIFK
QATMKALRKKSVLLTGYLEYLIKHNYGKDKAATKKPVVNIITPSHVEERGCQLTITFSVPNKDVFQELEK
RGVVCDKRNPNGIRVAPVPLYNSFHDVYKFTNLLTSILDSAETKN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003937
ORF Size 1395 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003937.3
RefSeq Size 1688 bp
RefSeq ORF 1398 bp
Locus ID 8942
UniProt ID Q16719
Cytogenetics 2q22.2
Protein Families Protease
Protein Pathways Metabolic pathways, Tryptophan metabolism
MW 52.2 kDa
Gene Summary Kynureninase is a pyridoxal-5'-phosphate (pyridoxal-P) dependent enzyme that catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. Kynureninase is involved in the biosynthesis of NAD cofactors from tryptophan through the kynurenine pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]

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