KDM5B (NM_006618) Human Tagged ORF Clone

CAT#: RC214918

KDM5B (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 5B (KDM5B)

Product Images

Circular map for RC214918

• 

  Western blot validation of overexpression lysate (Cat# LY401985) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214918 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KDM5B
• Synonyms: CT31; JARID1B; MRT65; PLU-1; PLU1; PPP1R98; PUT1; RBBP2H1A; RBP2-H1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214918 representing NM_006618
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGGCCACCACACTGCACCCAGGCCCGCGCCCGGCGCTGCCCCTCGGGGGCCCGGGCCCGCTGG
  GCGAGTTCCTGCCTCCACCCGAGTGCCCGGTCTTCGAACCCAGCTGGGAAGAGTTCGCGGACCCCTTCGC
  TTTCATCCACAAGATCCGGCCCATAGCCGAGCAGACTGGCATCTGTAAGGTGCGGCCGCCGCCGGATTGG
  CAGCCACCATTTGCATGTGATGTTGATAAACTTCATTTTACGCCACGTATCCAGAGACTGAATGAATTGG
  AGGCCCAAACTCGTGTAAAATTGAATTTCTTGGACCAGATTGCAAAGTACTGGGAGTTACAGGGAAGTAC
  TCTGAAAATTCCACATGTGGAGAGGAAGATCTTGGACTTATTTCAGCTTAATAAGTTAGTTGCAGAAGAA
  GGTGGATTTGCAGTTGTTTGCAAGGATAGAAAATGGACCAAAATTGCTACCAAGATGGGGTTTGCTCCTG
  GCAAAGCAGTGGGCTCACATATCAGAGGGCATTATGAACGAATTCTCAACCCCTACAACTTATTCCTGTC
  CGGAGACAGCCTAAGGTGTTTGCAGAAGCCAAACCTGACCACAGACACTAAGGACAAGGAGTACAAACCC
  CATGATATTCCCCAGAGGCAGTCTGTGCAGCCTTCGGAAACGTGCCCCCCAGCCCGACGAGCAAAACGCA
  TGAGAGCAGAGGCCATGAATATTAAAATAGAACCCGAGGAGACAACAGAAGCCAGAACTCATAATCTGAG
  ACGTCGAATGGGTTGTCCAACTCCAAAATGTGAAAATGAGAAAGAAATGAAGAGTAGCATCAAGCAAGAA
  CCTATTGAGAGGAAAGATTATATTGTAGAAAATGAGAAGGAAAAGCCCAAGAGTCGATCTAAAAAAGCCA
  CCAATGCTGTGGACCTGTATGTCTGTCTTTTATGTGGCAGTGGCAATGATGAAGACCGGCTACTGTTGTG
  TGATGGCTGTGATGACAGTTACCATACCTTTTGCTTGATCCCACCTCTCCATGATGTTCCCAAGGGAGAC
  TGGAGGTGTCCTAAGTGTTTGGCTCAGGAATGTAGTAAGCCACAAGAAGCATTTGGCTTTGAACAAGCAG
  CCAGGGACTATACCCTCCGTACTTTTGGGGAAATGGCAGATGCGTTCAAATCTGATTACTTCAACATGCC
  AGTCCATATGGTCCCCACAGAGCTTGTTGAGAAAGAATTTTGGAGACTAGTAAGCACTATTGAGGAGGAT
  GTCACAGTGGAATATGGAGCTGACATTGCCTCAAAGGAATTTGGCAGTGGCTTTCCTGTCCGAGATGGGA
  AAATCAAACTCTCACCTGAGGAAGAGGAGTATCTTGATAGTGGCTGGAATTTGAACAACATGCCAGTGAT
  GGAGCAGTCTGTCCTTGCACATATTACTGCTGATATATGTGGCATGAAACTTCCTTGGTTGTATGTGGGA
  ATGTGCTTTTCTTCATTCTGTTGGCACATTGAAGACCACTGGAGCTATTCAATTAACTACTTGCACTGGG
  GTGAGCCAAAAACCTGGTATGGAGTCCCAGGGTATGCTGCTGAGCAGCTAGAAAATGTAATGAAGAAACT
  AGCTCCAGAACTCTTTGTGTCCCAGCCGGATCTCCTCCATCAGCTTGTGACCATCATGAACCCCAATACC
  CTGATGACTCATGAAGTGCCTGTTTACCGAACTAATCAGTGTGCTGGGGAGTTTGTGATTACATTTCCAA
  GAGCCTACCACAGTGGTTTTAACCAGGGTTTTAATTTTGCTGAGGCTGTTAACTTCTGCACTGTTGATTG
  GCTGCCATTAGGCCGACAGTGTGTGGAGCATTATCGCTTGCTTCATCGATATTGTGTGTTTTCCCATGAT
  GAGATGATCTGCAAGATGGCTTCCAAGGCTGATGTATTAGATGTTGTAGTGGCTTCAACTGTTCAGAAAG
  ACATGGCCATTATGATTGAGGATGAGAAAGCTTTAAGAGAAACTGTCCGTAAATTGGGAGTGATTGATTC
  GGAAAGAATGGATTTTGAGCTGTTGCCAGATGATGAACGTCAGTGTGTAAAATGCAAAACTACATGCTTC
  ATGTCTGCCATCTCCTGTTCTTGTAAACCTGGCCTTCTTGTTTGCCTGCATCATGTAAAAGAATTGTGTT
  CCTGTCCTCCTTATAAATATAAATTGCGGTATAGGTACACGCTGGATGATCTCTACCCTATGATGAATGC
  ATTGAAGCTTCGAGCAGAATCTTACAACGAATGGGCCTTGAATGTGAATGAAGCTTTGGAGGCAAAGATC
  AACAAGAAGAAAAGCCTTGTCAGCTTCAAGGCTTTAATTGAAGAATCTGAAATGAAGAAATTCCCAGACA
  ATGATCTTTTGCGACACCTTCGCCTAGTCACACAGGATGCAGAGAAGTGTGCCTCTGTTGCGCAGCAGTT
  GCTTAATGGCAAAAGGCAAACTAGATATCGATCTGGTGGAGGGAAATCCCAAAATCAGTTGACAGTGAAT
  GAGCTCCGGCAGTTTGTAACACAGCTGTATGCTCTTCCATGTGTCCTCAGTCAGACACCATTACTAAAGG
  ATCTCTTGAATCGTGTAGAAGATTTTCAACAGCATAGTCAGAAACTACTCTCTGAGGAAACGCCTAGTGC
  TGCGGAGCTGCAGGACTTGCTAGATGTCAGCTTTGAATTTGATGTTGAACTTCCACAGCTTGCTGAGATG
  CGTATCCGTTTGGAACAAGCCCGTTGGCTAGAAGAGGTGCAGCAAGCTTGCCTAGACCCCAGCTCCCTTA
  CTTTAGATGATATGAGACGTCTCATAGACCTAGGGGTAGGGCTGGCCCCGTATTCAGCAGTGGAGAAAGC
  TATGGCCCGGCTGCAGGAACTGCTCACAGTGTCAGAGCACTGGGACGACAAAGCCAAGAGTCTCCTCAAG
  GCCAGGCCACGACATTCATTGAATAGCCTTGCTACGGCAGTAAAGGAAATCGAAGAGATCCCTGCATATC
  TGCCCAATGGTGCGGCTCTGAAAGACTCAGTGCAGAGAGCCAGAGACTGGCTTCAGGATGTAGAGGGCCT
  GCAGGCTGGAGGACGTGTGCCAGTGTTAGACACACTCATAGAACTTGTTACACGAGGCCGATCTATCCCG
  GTACATCTGAATTCTTTGCCAAGACTGGAAACCCTAGTAGCTGAGGTTCAGGCTTGGAAAGAATGTGCTG
  TTAATACATTCTTGACTGAGAATTCTCCATATTCTCTCTTAGAGGTGCTGTGTCCTCGATGTGATATTGG
  CCTTTTGGGATTGAAAAGGAAGCAGAGAAAGTTAAAGGAGCCCTTGCCAAATGGAAAGAAAAAAAGCACC
  AAATTAGAGAGTCTGAGTGACCTGGAGAGAGCTTTAACTGAAAGCAAGGAGACTGCTTCAGCTATGGCAA
  CTCTTGGGGAAGCTCGCCTAAGGGAAATGGAAGCCTTGCAGTCTCTCAGACTCGCCAATGAAGGGAAATT
  GCTGTCGCCTCTCCAAGATGTGGATATAAAAATCTGCCTATGTCAGAAGGCCCCAGCTGCCCCTATGATT
  CAATGTGAACTCTGCAGGGATGCTTTCCACACCAGTTGTGTGGCGGTACCCAGTATTTCACAGGGCCTGC
  GAATCTGGCTTTGTCCCCATTGTCGGAGGTCAGAGAAACCTCCATTAGAGAAAATTCTGCCCCTGCTCGC
  CTCCCTTCAGCGTATCCGAGTTCGCCTTCCTGAGGGAGATGCACTTCGATATATGATTGAAAGAACCGTG
  AACTGGCAGCACAGAGCCCAGCAACTGCTTTCGTCAGGGAATCTTAAATTTGTGCAAGATCGAGTGGGCT
  CAGGACTGTTATATAGCAGATGGCAAGCCTCAGCAGGACAGGTGTCAGACACAAACAAGGTATCTCAACC
  TCCTGGCACAACATCATTTTCTTTGCCTGATGACTGGGACAACAGAACCTCATATTTGCACTCCCCCTTC
  TCAACTGGACGAAGTTGTATCCCCCTCCATGGTGTTAGTCCAGAAGTGAATGAACTATTGATGGAAGCCC
  AGCTGCTCCAGGTATCCCTTCCTGAAATTCAGGAACTTTACCAGACTTTACTTGCAAAGCCAAGCCCTGC
  TCAGCAGACTGACCGAAGCTCACCAGTGAGACCCAGCAGTGAGAAGAATGACTGTTGCCGAGGGAAGCGA
  GATGGAATTAACAGTCTTGAGAGAAAACTGAAGAGACGCCTGGAAAGAGAGGGCCTCTCCAGTGAGCGGT
  GGGAACGAGTTAAGAAAATGCGGACCCCCAAAAAGAAGAAAATCAAACTGAGCCACCCCAAGGACATGAA
  CAATTTCAAGTTAGAGAGAGAGCGTAGCTATGAATTAGTTCGTTCTGCTGAAACTCATTCCCTGCCCTCA
  GACACATCCTATTCCGAACAGGAAGACTCTGAGGATGAAGATGCCATCTGCCCAGCTGTGAGCTGCCTGC
  AGCCAGAAGGAGATGAGGTGGACTGGGTCCAGTGTGATGGCAGCTGCAATCAGTGGTTTCATCAGGTCTG
  TGTTGGTGTCTCCCCAGAGATGGCAGAGAAAGAAGACTACATCTGTGTGCGCTGTACTGTGAAGGACGCA
  CCAAGCCGAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214918 representing NM_006618
  Red=Cloning site Green=Tags(s)
  
  MEAATTLHPGPRPALPLGGPGPLGEFLPPPECPVFEPSWEEFADPFAFIHKIRPIAEQTGICKVRPPPDW
  QPPFACDVDKLHFTPRIQRLNELEAQTRVKLNFLDQIAKYWELQGSTLKIPHVERKILDLFQLNKLVAEE
  GGFAVVCKDRKWTKIATKMGFAPGKAVGSHIRGHYERILNPYNLFLSGDSLRCLQKPNLTTDTKDKEYKP
  HDIPQRQSVQPSETCPPARRAKRMRAEAMNIKIEPEETTEARTHNLRRRMGCPTPKCENEKEMKSSIKQE
  PIERKDYIVENEKEKPKSRSKKATNAVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLIPPLHDVPKGD
  WRCPKCLAQECSKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVHMVPTELVEKEFWRLVSTIEED
  VTVEYGADIASKEFGSGFPVRDGKIKLSPEEEEYLDSGWNLNNMPVMEQSVLAHITADICGMKLPWLYVG
  MCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPGYAAEQLENVMKKLAPELFVSQPDLLHQLVTIMNPNT
  LMTHEVPVYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCVEHYRLLHRYCVFSHD
  EMICKMASKADVLDVVVASTVQKDMAIMIEDEKALRETVRKLGVIDSERMDFELLPDDERQCVKCKTTCF
  MSAISCSCKPGLLVCLHHVKELCSCPPYKYKLRYRYTLDDLYPMMNALKLRAESYNEWALNVNEALEAKI
  NKKKSLVSFKALIEESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVN
  ELRQFVTQLYALPCVLSQTPLLKDLLNRVEDFQQHSQKLLSEETPSAAELQDLLDVSFEFDVELPQLAEM
  RIRLEQARWLEEVQQACLDPSSLTLDDMRRLIDLGVGLAPYSAVEKAMARLQELLTVSEHWDDKAKSLLK
  ARPRHSLNSLATAVKEIEEIPAYLPNGAALKDSVQRARDWLQDVEGLQAGGRVPVLDTLIELVTRGRSIP
  VHLNSLPRLETLVAEVQAWKECAVNTFLTENSPYSLLEVLCPRCDIGLLGLKRKQRKLKEPLPNGKKKST
  KLESLSDLERALTESKETASAMATLGEARLREMEALQSLRLANEGKLLSPLQDVDIKICLCQKAPAAPMI
  QCELCRDAFHTSCVAVPSISQGLRIWLCPHCRRSEKPPLEKILPLLASLQRIRVRLPEGDALRYMIERTV
  NWQHRAQQLLSSGNLKFVQDRVGSGLLYSRWQASAGQVSDTNKVSQPPGTTSFSLPDDWDNRTSYLHSPF
  STGRSCIPLHGVSPEVNELLMEAQLLQVSLPEIQELYQTLLAKPSPAQQTDRSSPVRPSSEKNDCCRGKR
  DGINSLERKLKRRLEREGLSSERWERVKKMRTPKKKKIKLSHPKDMNNFKLERERSYELVRSAETHSLPS
  DTSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGVSPEMAEKEDYICVRCTVKDA
  PSRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006618
• ORF Size: 4632 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006618.5
• RefSeq Size: 6393 bp
• RefSeq ORF: 4635 bp
• Locus ID: 10765
• UniProt ID: Q9UGL1
• Cytogenetics: 1q32.1
• Domains: ARID, PHD, JmjC, JmjN, zf-C5HC2
• Protein Families: Druggable Genome, Transcription Factors
• MW: 175.5 kDa
• Gene Summary: This gene encodes a lysine-specific histone demethylase that belongs to the jumonji/ARID domain-containing family of histone demethylases. The encoded protein is capable of demethylating tri-, di- and monomethylated lysine 4 of histone H3. This protein plays a role in the transcriptional repression or certain tumor suppressor genes and is upregulated in certain cancer cells. This protein may also play a role in genome stability and DNA repair. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]