ZNF675 (NM_138330) Human Tagged ORF Clone

CAT#: RC214834

ZNF675 (Myc-DDK-tagged)-Human zinc finger protein 675 (ZNF675). Note: ORF is codon optimized

Product Images

Circular map for RC214834

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF675
• Synonyms: TBZF; TIZ
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214834 ORF sequence, codon optimized.
  Due to the complexity of NM_138330, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCTGCTTACCTTTAGGGACGTGGCGATCGAGTTTAGCTTGGAGGAATGGCAGTGCTTGGATACAG
  CCCAGCGAAACCTCTATAAGAACGTCATTCTCGAGAACTACAGAAATCTGGTTTTTCTCGGGATTGCAGT
  GAGTAAGCAGGACCTCATTACTTGTCTTGAACAGGAAAAAGAACCCCTGACCGTTAAGAGGCACGAGATG
  GTGAATGAGCCGCCAGTGATGTGCTCACACTTTGCCCAGGAGTTTTGGCCGGAGCAGAATATTAAAGACA
  GCTTTGAAAAGGTGACATTGCGGCGATATGAGAAGTGCGGCAACGATAACTTTCAGCTCAAAGGATGCAA
  GTCAGTCGACGAGTGCAAGCTCCATAAAGGGGGCTATAACGGCCTCAATCAGTGCCTTCCCACCATGCAG
  AGCAAAATGTTCCAGTGCGATAAGTATGTGAAAGTCTTCAATAAGTTTAGTCATTCTGACCGGCACAAAA
  TTAAGCACATGGAAAATAAGCCTTTCAAGTGTAAAGAATGCGGACGATCTTTTTGTATGCTGTCTCACCT
  GACACGGCACGAAAGAAACTATACAAAGGTCAATTTTTGCAAATGCGAGGAGTGCGAAAAAGCCGTAAAC
  CAGTCCTCCAAGTTGACCAAACATAAAAGAATCTACACATGTGAGAAGCTCTATAAGTGTCAGGAATGCG
  ATAGAACGTTCAACCAGTTTAGTAACCTTACAGAATATAAGAAAGATTACGCCCGAGAGAAACCGTATAA
  ATGTGAGGAATGTGGGAAGGCTTTTAATCAGAGCTCCCACCTTACCACTCACAAGATCATCCATACGGGG
  GAAAAGCCTTACAAGTGTGAGGAGTGCGGGAAAGCCTTCAACCAGTTTAGTAATTTGACTACTCACAAGA
  AGATCCATACTGGTGAGCAGCCGTATATCTGTGAGGAATGCGGTAAGGCATTTACACAGTCTTCTACCCT
  TACCACTCACAAACGCATACACACCGGCGAAAAGCCGTACAAGTGCGAGGAATGCGGCAAAGCGTTTAAC
  AGGTCCTCTAAGCTTACAGAACACAAGAACATTCATACCGGCGAGCAGCCATATAAATGCGAAGAGTGTG
  GGAAGGCGTTTAACCGCAGCAGCAACCTGACCGAACATCGAAAAATCCACACTGAAGAGAAGCCATACAA
  GTGTAAAGAATGTGGTAAAGCATTCAAACACTCTTCAGCCCTCACAACTCATAAAAGGATCCACACGGGA
  GAAAAACCTTACAAATGCGAGGAGTGCGGCAAGGCTTTCAACAGATCTTCAAAGCTGACCGAGCACAAGA
  AGCTTCATACAGGGAAGAAGCCCTACAAGTGTGAAGAATGTGGAAAAGCTTTTATTCAAAGCTCTAAGCT
  GACAGAACACAAAAAGATTCACTCCGGAGAGATTCCCTATAAATGCGAGGAATGTGGGAAAGCCTTCAAG
  CACTCCAGCAGCCTTACAACCCATAAACGCATCCACACAGGCGAGAAACCATATAAGTGCGAAGAGTGCG
  GCAAGGCATTTTCCAGGAGTAGCAAGCTGACCGAGCACAAAATCATCCATACCGGGGAGAAACCTTACAA
  ATGCGAGCGCTGCGACAAGGCCTTCAATCAATCCGCTAACCTGACCAAGCACAAGAAGATTCACACGGGA
  GAGAAATTGCAGAATTGGAATGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214834 representing NM_138330
  Red=Cloning site Green=Tags(s)
  
  MGLLTFRDVAIEFSLEEWQCLDTAQRNLYKNVILENYRNLVFLGIAVSKQDLITCLEQEKEPLTVKRHEM
  VNEPPVMCSHFAQEFWPEQNIKDSFEKVTLRRYEKCGNDNFQLKGCKSVDECKLHKGGYNGLNQCLPTMQ
  SKMFQCDKYVKVFNKFSHSDRHKIKHMENKPFKCKECGRSFCMLSHLTRHERNYTKVNFCKCEECEKAVN
  QSSKLTKHKRIYTCEKLYKCQECDRTFNQFSNLTEYKKDYAREKPYKCEECGKAFNQSSHLTTHKIIHTG
  EKPYKCEECGKAFNQFSNLTTHKKIHTGEQPYICEECGKAFTQSSTLTTHKRIHTGEKPYKCEECGKAFN
  RSSKLTEHKNIHTGEQPYKCEECGKAFNRSSNLTEHRKIHTEEKPYKCKECGKAFKHSSALTTHKRIHTG
  EKPYKCEECGKAFNRSSKLTEHKKLHTGKKPYKCEECGKAFIQSSKLTEHKKIHSGEIPYKCEECGKAFK
  HSSSLTTHKRIHTGEKPYKCEECGKAFSRSSKLTEHKIIHTGEKPYKCERCDKAFNQSANLTKHKKIHTG
  EKLQNWNV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138330
• ORF Size: 1704 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138330.1, NM_138330.2, NP_612203.1, NP_612203.2
• RefSeq Size: 2209 bp
• RefSeq ORF: 1707 bp
• Locus ID: 171392
• UniProt ID: Q8TD23
• Cytogenetics: 19p12
• Domains: KRAB, zf-C2H2
• Protein Families: Transcription Factors
• MW: 66.3 kDa
• Gene Summary: May be involved in transcriptional regulation. May play a role during osteoclast differentiation by modulating TRAF6 signaling activity.[UniProtKB/Swiss-Prot Function]