CRMP3 (DPYSL4) (NM_006426) Human Tagged ORF Clone

CAT#: RC214821

DPYSL4 (Myc-DDK-tagged)-Human dihydropyrimidinase-like 4 (DPYSL4)

Product Images

Western blot validation of overexpression lysate (Cat# LY416651) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214821 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified DPYSL4 protein (Cat# TP314821). The protein was produced from HEK293T cells transfected with DPYSL4 cDNA clone (Cat# RC214821) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CRMP3
• Synonyms: CRMP3; DRP-4; ULIP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214821 representing NM_006426.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCCTTCCAGGGCAAGAAAAGCATCCCCCGGATCACGAGTGACCGCCTTCTGATCAGAGGTGGGAGG
  ATCGTGAATGACGACCAGTCCTTTTACGCTGATGTGCACGTGGAAGATGGCTTGATAAAACAAATCGGA
  GAAAACCTCATCGTCCCTGGGGGCATCAAGACCATTGACGCCCACGGCCTGATGGTCCTTCCTGGTGGC
  GTTGACGTCCACACAAGGCTGCAGATGCCTGTCCTGGGCATGACACCGGCTGACGACTTCTGTCAGGGC
  ACCAAGGCAGCGCTAGCAGGAGGAACCACCATGATCTTGGACCACGTCTTCCCCGACACGGGTGTGAGC
  CTGCTGGCGGCCTACGAGCAGTGGCGGGAGCGGGCGGACAGCGCGGCCTGCTGCGACTACTCCCTGCAC
  GTGGACATCACCCGATGGCATGAGAGCATCAAGGAGGAGCTGGAGGCCCTGGTCAAGGAGAAGGGTGTG
  AACTCCTTCCTGGTCTTCATGGCATACAAGGACCGGTGCCAGTGCAGCGACAGCCAGATGTACGAGATC
  TTCAGCATCATCCGGGACCTGGGGGCCTTGGCCCAGGTGCACGCTGAGAACGGGGACATCGTGGAGGAG
  GAGCAGAAGCGGTTGCTGGAGCTCGGCATCACTGGCCCCGAGGGCCACGTGCTCAGCCACCCCGAGGAG
  GTGGAGGCTGAGGCGGTGTACCGAGCTGTCACCATCGCCAAGCAGGCAAACTGCCCGCTGTACGTCACC
  AAGGTGATGAGCAAGGGGGCGGCCGACGCCATCGCTCAGGCCAAGCGCAGAGGGGTGGTCGTGTTTGGG
  GAGCCCATCACCGCCAGCCTGGGCACCGACGGTTCACACTACTGGAGCAAGAACTGGGCCAAGGCCGCA
  GCCTTCGTCACATCACCCCCTGTCAACCCAGACCCCACCACGGCAGACCACCTCACCTGCTTGCTGTCC
  AGCGGGGACCTCCAGGTGACAGGCAGCGCCCACTGCACCTTCACCACTGCCCAGAAGGCTGTGGGCAAG
  GACAACTTCGCGCTGATCCCCGAGGGCACCAACGGCATTGAGGAGCGCATGTCGATGGTCTGGGAGAAA
  TGTGTGGCCTCTGGGAAGATGGACGAGAATGAGTTCGTCGCGGTGACCAGTACAAATGCTGCCAAAATC
  TTCAATTTTTACCCAAGGAAGGGGCGAGTGGCTGTGGGCTCTGACGCTGACCTGGTCATATGGAACCCC
  AAGGCCACCAAGATCATCTCTGCCAAGACCCACAATCTGAACGTGGAGTACAACATCTTCGAGGGAGTG
  GAGTGCCGGGGAGCGCCTGCCGTGGTCATAAGTCAGGGCCGAGTGGCGCTGGAGGACGGGAAGATGTTT
  GTCACCCCGGGGGCGGGCCGCTTCGTCCCTCGGAAAACATTCCCGGACTTTGTCTACAAGAGGATCAAA
  GCTCGCAACAGGCTGGCGGAGATCCACGGTGTGCCCCGTGGGCTGTATGACGGGCCCGTCCACGAGGTG
  ATGGTGCCTGCCAAGCCAGGGAGTGGCGCTCCGGCCCGCGCGTCCTGCCCAGGCAAGATCTCCGTCCCT
  CCTGTGCGCAACCTACATCAGTCGGGGTTCAGCCTATCTGGGTCTCAGGCTGATGACCACATCGCCCGA
  CGCACAGCACAGAAGATCATGGCACCACCTGGCGGCCGCTCCAACATCACCTCTCTCTCC
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by RC214821
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSFQGKKSIPRITSDRLLIRGGRIVNDDQSFYADVHVEDGLIKQIGENLIVPGGIKTIDAHGLMVLPGG
  VDVHTRLQMPVLGMTPADDFCQGTKAALAGGTTMILDHVFPDTGVSLLAAYEQWRERADSAACCDYSLH
  VDITRWHESIKEELEALVKEKGVNSFLVFMAYKDRCQCSDSQMYEIFSIIRDLGALAQVHAENGDIVEE
  EQKRLLELGITGPEGHVLSHPEEVEAEAVYRAVTIAKQANCPLYVTKVMSKGAADAIAQAKRRGVVVFG
  EPITASLGTDGSHYWSKNWAKAAAFVTSPPVNPDPTTADHLTCLLSSGDLQVTGSAHCTFTTAQKAVGK
  DNFALIPEGTNGIEERMSMVWEKCVASGKMDENEFVAVTSTNAAKIFNFYPRKGRVAVGSDADLVIWNP
  KATKIISAKTHNLNVEYNIFEGVECRGAPAVVISQGRVALEDGKMFVTPGAGRFVPRKTFPDFVYKRIK
  ARNRLAEIHGVPRGLYDGPVHEVMVPAKPGSGAPARASCPGKISVPPVRNLHQSGFSLSGSQADDHIAR
  RTAQKIMAPPGGRSNITSLS
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Recombinant protein using RC214821 also available, TP314821
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006426
• ORF Size: 1716 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006426.3
• RefSeq Size: 2699 bp
• RefSeq ORF: 1719 bp
• Locus ID: 10570
• UniProt ID: O14531
• Cytogenetics: 10q26.3
• Domains: Amidohydro_1
• MW: 61.9 kDa
• Gene Summary: Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration (By similarity).[UniProtKB/Swiss-Prot Function]