MAML1 (NM_014757) Human Tagged ORF Clone

CAT#: RC214790

MAML1 (Myc-DDK-tagged)-Human mastermind-like 1 (Drosophila) (MAML1)

Product Images

Circular map for RC214790

• 

  Western blot validation of overexpression lysate (Cat# LY415055) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214790 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified MAML1 protein (Cat# TP314790). The protein was produced from HEK293T cells transfected with MAML1 cDNA clone (Cat# RC214790) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MAML1
• Synonyms: Mam-1; Mam1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214790 representing NM_014757
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCTGCCCACCTGCCCCATGGCGGAGTTCGCGCTGCCGCGGCACAGCGCGGTCATGGAGCGCCTTC
  GCCGGCGCATCGAGCTGTGCCGGCGCCACCACAGCACCTGCGAGGCCCGCTACGAGGCCGTGTCGCCCGA
  GCGCCTGGAGCTGGAGCGCCAACACACCTTCGCCCTGCACCAGCGCTGCATCCAGGCCAAGGCCAAGCGC
  GCCGGGAAGCACAGGCAGCCGCCCGCCGCCACGGCCCCGGCGCCCGCCGCCCCGGCCCCGCGCCTGGACG
  CCGCTGACGGCCCCGAGCACGGCCGCCCGGCCACGCATCTTCATGATACAGTTAAGAGGAATCTTGACAG
  CGCCACTTCCCCTCAGAATGGCGATCAACAGAATGGCTACGGGGACCTCTTTCCTGGGCATAAGAAGACT
  CGCCGGGAGGCCCCTCTGGGAGTTGCCATCTCTTCCAATGGACTGCCTCCAGCCTCCCCCCTCGGTCAGT
  CTGACAAGCCTTCTGGAGCCGACGCCCTGCAGTCCAGTGGGAAGCACTCTCTGGGGCTAGACTCTCTCAA
  CAAAAAGCGTCTGGCTGACTCCAGCCTTCACTTGAATGGAGGCAGTAACCCCAGTGAGTCATTTCCTCTG
  AGCCTGAATAAAGAACTGAAGCAGGAGCCTGTCGAAGACCTGCCTTGCATGATCACTGGGACTGTCGGCT
  CCATATCGCAAAGCAACCTCATGCCAGACCTCAACCTTAACGAGCAGGAGTGGAAGGAGCTCATCGAGGA
  GCTGAACAGGTCGGTGCCCGATGAAGACATGAAGGACCTGTTTAATGAGGACTTCGAGGAGAAGAAGGAC
  CCAGAGTCTTCTGGCTCTGCCACACAAACCCCCTTGGCACAGGACATTAATATTAAGACGGAATTCTCTC
  CAGCAGCCTTTGAGCAAGAACAGTTAGGCTCTCCACAAGTGAGGGCCGGGTCTGCAGGGCAGACCTTTCT
  GGGGCCTTCCTCTGCCCCTGTGAGTACAGATTCCCCCAGCCTAGGGGGCTCCCAAACCTTATTCCACACC
  TCTGGTCAGCCCCGGGCGGACAATCCCAGTCCAAACCTGATGCCGGCATCAGCCCAGGCCCAGAACGCAC
  AAAGAGCCCTTGCAGGTGTGGTATTGCCCAGTCAGGGCCCAGGAGGGGCCTCAGAGCTGTCCTCTGCCCA
  CCAGCTCCAGCAGATCGCTGCCAAGCAGAAGCGCGAGCAGATGCTCCAGAACCCACAGCAGGCCACCCCG
  GCACCAGCCCCGGGCCAGATGTCCACATGGCAGCAGACGGGGCCCTCCCACAGTTCCTTAGATGTCCCTT
  ACCCCATGGAGAAGCCTGCCAGCCCTTCCAGCTACAAGCAAGACTTCACTAACTCCAAACTGCTCATGAT
  GCCTAGTGTGAATAAGAGTTCCCCTCGGCCCGGAGGCCCCTACCTCCAGCCCAGCCATGTGAACCTGCTG
  AGTCACCAGCCACCGAGTAACTTGAATCAGAACTCCGCGAATAACCAGGGGTCTGTGCTGGACTACGGCA
  ATACAAAACCCCTTTCTCATTACAAAGCGGACTGTGGGCAAGGCAGCCCGGGGTCTGGCCAGAGCAAGCC
  AGCCCTGATGGCTTATCTTCCCCAGCAGCTGTCCCATATAAGTCACGAGCAGAACTCCCTGTTTCTGATG
  AAGCCAAAGCCAGGAAATATGCCTTTCCGATCACTGGTTCCACCTGGCCAGGAGCAGAACCCTTCCAGTG
  TCCCTGTGCAAGCCCAGGCTACCAGTGTTGGGACCCAGCCGCCTGCCGTGTCCGTGGCCAGCTCCCACAA
  CAGCTCCCCCTATCTCAGCAGCCAGCAACAGGCCGCTGTAATGAAGCAGCATCAGTTGCTTTTGGACCAA
  CAGAAACAAAGGGAGCAGCAGCAAAAGCATTTACAGCAACAGCAGTTCCTTCAGAGGCAACAGCACCTTC
  TCGCGGAACAGGAGAAGCAACAGTTTCAGCGCCATCTGACCCGCCCACCACCCCAGTACCAAGACCCGAC
  ACAAGGCAGCTTCCCACAGCAGGTTGGACAGTTCACAGGGTCCTCTGCTGCCGTGCCCGGCATGAACACC
  TTGGGTCCATCCAACTCCAGCTGTCCTCGAGTGTTCCCTCAGGCTGGGAATCTGATGCCAATGGGCCCTG
  GACATGCTTCAGTTTCCTCTCTCCCCACAAACTCAGGCCAACAGGACCGGGGTGTGGCTCAGTTCCCTGG
  CTCCCAAAACATGCCTCAGAGCAGCCTCTATGGCATGGCTTCTGGCATAACCCAGATAGTTGCCCAGCCC
  CCGCCACAGGCCACCAATGGACATGCCCACATTCCACGGCAGACCAACGTGGGCCAGAACACCTCCGTCT
  CAGCTGCCTATGGGCAGAACTCTCTGGGAAGCTCTGGCCTCTCCCAGCAGCACAATAAGGGGACCCTGAA
  CCCTGGTTTAACAAAGCCACCGGTCCCAAGGGTGTCACCAGCCATGGGAGGCCAGAATTCCTCCTGGCAG
  CATCAGGGAATGCCGAACCTCAGTGGCCAGACCCCAGGGAACAGCAACGTGAGTCCCTTCACTGCAGCCT
  CCAGTTTCCACATGCAGCAGCAGGCCCACCTGAAAATGTCTAGCCCGCAATTCTCCCAGGCAGTGCCCAA
  CAGGCCCATGGCTCCCATGAGCTCAGCAGCTGCCGTGGGGTCCTTGCTACCCCCAGTGAGTGCACAGCAG
  AGGACCAGCGCCCCTGCCCCAGCACCACCCCCAACAGCCCCTCAGCAGGGCTTGCCTGGCCTGAGCCCAG
  CAGGGCCTGAGCTGGGGGCCTTCAGCCAGAGCCCTGCCTCACAGATGGGCGGTCGGGCGGGGCTGCACTG
  CACCCAGGCCTACCCTGTGCGGACCGCGGGCCAGGAGCTGCCTTTTGCCTATAGCGGGCAGCCAGGTGGC
  AGTGGGCTCTCTAGTGTGGCTGGACACACCGATCTGATCGACTCCCTGCTGAAGAACAGGACTTCAGAGG
  AGTGGATGAGTGATTTGGACGACCTGTTAGGGTCTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214790 representing NM_014757
  Red=Cloning site Green=Tags(s)
  
  MVLPTCPMAEFALPRHSAVMERLRRRIELCRRHHSTCEARYEAVSPERLELERQHTFALHQRCIQAKAKR
  AGKHRQPPAATAPAPAAPAPRLDAADGPEHGRPATHLHDTVKRNLDSATSPQNGDQQNGYGDLFPGHKKT
  RREAPLGVAISSNGLPPASPLGQSDKPSGADALQSSGKHSLGLDSLNKKRLADSSLHLNGGSNPSESFPL
  SLNKELKQEPVEDLPCMITGTVGSISQSNLMPDLNLNEQEWKELIEELNRSVPDEDMKDLFNEDFEEKKD
  PESSGSATQTPLAQDINIKTEFSPAAFEQEQLGSPQVRAGSAGQTFLGPSSAPVSTDSPSLGGSQTLFHT
  SGQPRADNPSPNLMPASAQAQNAQRALAGVVLPSQGPGGASELSSAHQLQQIAAKQKREQMLQNPQQATP
  APAPGQMSTWQQTGPSHSSLDVPYPMEKPASPSSYKQDFTNSKLLMMPSVNKSSPRPGGPYLQPSHVNLL
  SHQPPSNLNQNSANNQGSVLDYGNTKPLSHYKADCGQGSPGSGQSKPALMAYLPQQLSHISHEQNSLFLM
  KPKPGNMPFRSLVPPGQEQNPSSVPVQAQATSVGTQPPAVSVASSHNSSPYLSSQQQAAVMKQHQLLLDQ
  QKQREQQQKHLQQQQFLQRQQHLLAEQEKQQFQRHLTRPPPQYQDPTQGSFPQQVGQFTGSSAAVPGMNT
  LGPSNSSCPRVFPQAGNLMPMGPGHASVSSLPTNSGQQDRGVAQFPGSQNMPQSSLYGMASGITQIVAQP
  PPQATNGHAHIPRQTNVGQNTSVSAAYGQNSLGSSGLSQQHNKGTLNPGLTKPPVPRVSPAMGGQNSSWQ
  HQGMPNLSGQTPGNSNVSPFTAASSFHMQQQAHLKMSSPQFSQAVPNRPMAPMSSAAAVGSLLPPVSAQQ
  RTSAPAPAPPPTAPQQGLPGLSPAGPELGAFSQSPASQMGGRAGLHCTQAYPVRTAGQELPFAYSGQPGG
  SGLSSVAGHTDLIDSLLKNRTSEEWMSDLDDLLGSQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014757
• ORF Size: 3048 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014757.5
• RefSeq Size: 5745 bp
• RefSeq ORF: 3051 bp
• Locus ID: 9794
• UniProt ID: Q92585
• Cytogenetics: 5q35.3
• Protein Pathways: Notch signaling pathway
• MW: 107.9 kDa
• Gene Summary: This protein is the human homolog of mastermind, a Drosophila protein that plays a role in the Notch signaling pathway involved in cell-fate determination. There is in vitro evidence that the human homolog forms a complex with the intracellular portion of human Notch receptors and can increase expression of a Notch-induced gene. This evidence supports its proposed function as a transcriptional co-activator in the Notch signaling pathway. [provided by RefSeq, Jul 2008]