DAB1 (NM_021080) Human Tagged ORF Clone

CAT#: RC214664

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DAB1 (Myc-DDK-tagged)-Human disabled homolog 1 (Drosophila) (DAB1)



Reconstitution Protocol

USD 517.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol DAB1
Synonyms SCA37
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC214664 representing NM_021080
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAACTGAGACAGAACTTCAAGTAGCTGTGAAAACCAGCGCCAAGAAAGACTCCAGAAAGAAAGGTC
AGGATCGCAGTGAAGCCACTTTGATAAAGAGGTTTAAAGGTGAAGGGGTCCGGTACAAAGCCAAATTGAT
CGGGATTGATGAAGTTTCCGCAGCTCGGGGAGACAAGTTATGTCAAGATTCCATGATGAAACTCAAGGGC
GTTGTTGCTGGCGCTCGTTCCAAAGGAGAACACAAACAGAAAATCTTTTTAACCATCTCCTTTGGAGGAA
TCAAAATCTTTGATGAGAAGACAGGGGCCCTTCAGCATCATCATGCTGTTCATGAAATATCCTACATTGC
AAAGGACATTACAGATCACCGGGCCTTTGGATATGTTTGTGGGAAGGAAGGGAATCACAGATTTGTGGCC
ATAAAAACAGCCCAGGCGGCTGAACCTGTTATTCTGGACTTGAGAGATCTCTTTCAACTCATTTATGAAT
TGAAGCAAAGAGAAGAATTAGAAAAAAAGGCACAAAAGGATAAGCAGTGTGAACAAGCTGTGTACCAGAC
AATATTGGAAGAGGATGTTGAAGATCCTGTGTACCAGTACATTGTGTTTGAGGCTGGACACGAGCCAATC
CGTGATCCCGAAACGGAAGAAAACATTTATCAGGTTCCCACCAGCCAAAAGAAGGAAGGTGTTTATGATG
TGCCAAAAAGTCAACCTGTAAGTGCTGTGACCCAATTAGAACTTTTTGGGGACATGTCCACACCCCCTGA
TATAACCTCTCCCCCCACTCCTGCAACTCCAGGTGATGCCTTTATCCCATCTTCATCTCAGACCCTTCCA
GCGAGTGCAGATGTGTTTAGTTCTGTACCTTTCGGCACTGCTGCTGTACCCTCAGGTTACGTTGCAATGG
GCGCTGTCCTCCCGTCCTTCTGGGGTCAGCAGCCCCTCGTCCAACAGCAGATGGTCATGGGTGCCCAGCC
ACCAGTCGCTCAGGTGATGCCGGGGGCTCAGCCCATCGCATGGGGCCAGCCGGGTCTCTTTCCTGCCACT
CAGCAGCCCTGGCCAACTGTGGCCGGGCAGTTTCCGCCAGCCGCCTTCATGCCCACACAAACTGTTATGC
CTTTGCCAGCTGCCATGTTCCAAGGTCCCCTCACCCCCCTTGCCACCGTCCCAGGCACGAGTGACTCCAC
CAGGTCAAGTCCACAGACCGACAAGCCCAGGCAGAAAATGGGCAAAGAAACGTTTAAGGATTTCCAGATG
GCCCAGCCTCCGCCCGTGCCCTCCCGCAAACCCGACCAGCCCTCCCTCACCTGTACCTCAGAGGCCTTCT
CCAGTTACTTCAACAAAGTCGGGGTGGCACAGGATACAGACGACTGTGATGACTTTGACATCTCCCAGTT
GAATTTGACCCCTGTGACTTCTACCACACCATCGACCAACTCACCTCCAACCCCAGCCCCTAGACAGAGC
TCTCCATCCAAATCATCTGCATCCCATGCCAGTGATCCTACCACAGATGACATCTTTGAAGAGGGCTTTG
AAAGTCCCAGCAAAAGCGAAGAGCAAGAAGCTCCTGATGGATCACAGGCCTCATCCAACAGTGATCCATT
TGGTGAGCCCAGTGGGGAGCCCAGTGGTGATAATATAAGTCCACAGGCCGGTAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC214664 representing NM_021080
Red=Cloning site Green=Tags(s)

MSTETELQVAVKTSAKKDSRKKGQDRSEATLIKRFKGEGVRYKAKLIGIDEVSAARGDKLCQDSMMKLKG
VVAGARSKGEHKQKIFLTISFGGIKIFDEKTGALQHHHAVHEISYIAKDITDHRAFGYVCGKEGNHRFVA
IKTAQAAEPVILDLRDLFQLIYELKQREELEKKAQKDKQCEQAVYQTILEEDVEDPVYQYIVFEAGHEPI
RDPETEENIYQVPTSQKKEGVYDVPKSQPVSAVTQLELFGDMSTPPDITSPPTPATPGDAFIPSSSQTLP
ASADVFSSVPFGTAAVPSGYVAMGAVLPSFWGQQPLVQQQMVMGAQPPVAQVMPGAQPIAWGQPGLFPAT
QQPWPTVAGQFPPAAFMPTQTVMPLPAAMFQGPLTPLATVPGTSDSTRSSPQTDKPRQKMGKETFKDFQM
AQPPPVPSRKPDQPSLTCTSEAFSSYFNKVGVAQDTDDCDDFDISQLNLTPVTSTTPSTNSPPTPAPRQS
SPSKSSASHASDPTTDDIFEEGFESPSKSEEQEAPDGSQASSNSDPFGEPSGEPSGDNISPQAGS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_021080
ORF Size 1665 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_021080.5
RefSeq Size 2580 bp
RefSeq ORF 1668 bp
Locus ID 1600
UniProt ID O75553
Cytogenetics 1p32.2
Domains PID
MW 59.8 kDa
Gene Summary The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer. This gene is similar to disabled-1, and the protein encoded by this gene is thought to be a signal transducer that interacts with protein kinase pathways to regulate neuronal positioning in the developing brain. [provided by RefSeq, Jan 2017]
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