HMGA2 (NM_003483) Human Tagged ORF Clone
HMGA2 (Myc-DDK-tagged)-Human high mobility group AT-hook 2 (HMGA2), transcript variant 1
|Type||Human Tagged ORF Clone|
|Synonyms||BABL; HMGI-C; HMGIC; LIPO; SRS5; STQTL9|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC214629 representing NM_003483
Red=Cloning site Blue=ORF Green=Tags(s)
>RC214629 representing NM_003483
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||327 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||4150 bp|
|RefSeq ORF||330 bp|
|Protein Families||Druggable Genome|
|Gene Summary||This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]|
|cDNA Clone Resources|
|RC214629L1||Lenti ORF clone of Human high mobility group AT-hook 2 (HMGA2), transcript variant 1, Myc-DDK-tagged||
|RC214629L2||Lenti ORF clone of Human high mobility group AT-hook 2 (HMGA2), transcript variant 1, mGFP tagged||
|RC214629L3||Lenti ORF clone of Human high mobility group AT-hook 2 (HMGA2), transcript variant 1, Myc-DDK-tagged||
|RC214629L4||Lenti ORF clone of Human high mobility group AT-hook 2 (HMGA2), transcript variant 1, mGFP tagged||
|RG214629||HMGA2 (GFP-tagged) - Human high mobility group AT-hook 2 (HMGA2), transcript variant 1||
|SC303313||HMGA2 (untagged)-Human high mobility group AT-hook 2 (HMGA2), transcript variant 1||