Desmocollin 1 (DSC1) (NM_024421) Human Tagged ORF Clone

CAT#: RC214491

DSC1 (Myc-DDK-tagged)-Human desmocollin 1 (DSC1), transcript variant Dsc1a

Product Images

Circular map for RC214491

• 

  Western blot validation of overexpression lysate (Cat# LY411285) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214491 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Desmocollin 1
• Synonyms: CDHF1; DG2/DG3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214491 representing NM_024421
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGGCCTCTGCTGCCCCAGGGAGCATCTTCTGTAAGCAGCTCCTTTTCTCTCTCCTGGTTTTAA
  CATTACTTTGCGATGCTTGTCAGAAAGTTTATCTTCGAGTTCCTTCTCATCTTCAGGCTGAAACACTTGT
  AGGCAAAGTGAATCTGGAGGAGTGTCTCAAGTCGGCCAGCCTAATCCGGTCCAGTGACCCTGCCTTCAGA
  ATTCTAGAAGATGGCTCAATTTACACAACACATGACCTCATTTTGTCTTCTGAAAGGAAAAGTTTTTCCA
  TTTTCCTTTCAGATGGTCAGAGACGGGAACAACAAGAGATAAAAGTTGTACTGTCAGCAAGAGAAAACAA
  GTCTCCTAAGAAGAGACATACCAAAGACACAGCCCTCAAGCGCAGCAAGAGACGATGGGCTCCTATTCCA
  GCTTCATTGATGGAGAACTCGTTGGGTCCATTTCCACAACACGTTCAGCAGATCCAATCTGATGCTGCAC
  AGAATTACACCATCTTTTATTCCATAAGTGGGCCAGGCGTGGACAAAGAACCCTTCAATTTGTTTTACAT
  AGAGAAAGACACTGGGGATATCTTTTGTACAAGGAGCATTGACCGTGAGAAATATGAACAGTTTGCGTTA
  TATGGCTATGCAACAACTGCAGATGGCTATGCACCAGAATATCCACTCCCTTTGATCATCAAAATTGAAG
  ATGATAATGATAACGCCCCATATTTTGAACACAGAGTGACTATCTTTACTGTGCCTGAAAATTGCCGATC
  CGGAACTTCAGTGGGAAAAGTGACCGCCACAGACCTTGACGAACCTGACACTCTCCATACTCGTCTGAAA
  TATAAAATCTTACAACAAATCCCAGATCATCCAAAGCATTTCTCCATACACCCAGATACCGGTGTCATCA
  CCACAACTACACCTTTTCTGGATAGAGAAAAATGTGATACTTACCAGTTAATAATGGAAGTGCGAGACAT
  GGGTGGTCAGCCTTTCGGTTTATTTAATACAGGAACAATTACTATTTCACTTGAGGATGAAAATGACAAT
  CCACCATCTTTCACAGAAACTTCTTATGTTACAGAAGTAGAAGAAAACAGAATTGACGTGGAGATTTTAC
  GAATGAAGGTACAGGATCAGGATTTGCCAAACACTCCTCACTCAAAGGCTGTATACAAAATCCTACAAGG
  AAATGAAAATGGAAACTTCATAATTAGCACAGATCCAAATACAAATGAAGGAGTGCTGTGTGTTGTCAAG
  CCATTGAACTATGAAGTCAATCGCCAAGTTATTTTGCAAGTTGGTGTCATTAACGAGGCACAATTCTCTA
  AAGCAGCGAGCTCACAAACTCCTACAATGTGCACTACAACTGTCACCGTTAAAATTATAGACAGTGATGA
  GGGCCCTGAATGCCACCCTCCAGTGAAAGTTATTCAGAGTCAAGATGGCTTCCCAGCTGGCCAAGAACTC
  CTTGGATACAAAGCACTGGACCCGGAAATATCCAGTGGTGAAGGCTTAAGGTATCAGAAGTTAGGGGATG
  AAGATAACTGGTTTGAAATTAATCAACACACTGGCGACTTGAGAACTCTAAAAGTACTAGATAGAGAATC
  CAAATTTGTAAAAAACAACCAATACAATATTTCAGTTGTTGCAGTGGATGCAGTTGGCCGATCTTGCACT
  GGAACATTAGTAGTTCATTTGGATGATTACAACGATCACGCACCTCAAATTGACAAAGAAGTGACCATTT
  GTCAGAATAATGAGGATTTTGCTGTTCTGAAACCTGTAGATCCAGATGGACCTGAAAATGGACCACCTTT
  TCAATTCTTTCTGGATAATTCTGCCAGTAAAAACTGGAACATAGAAGAAAAGGATGGTAAAACTGCCATT
  CTTCGTCAACGGCAAAATCTTGATTATAACTATTATTCTGTGCCTATTCAAATAAAAGACAGGCATGGTT
  TAGTTGCAACACATATGTTAACAGTGAGAGTATGTGACTGTTCAACTCCATCTGAGTGTAGAATGAAGGA
  TAAAAGTACAAGAGACGTTAGACCAAATGTAATACTTGGAAGATGGGCTATTCTTGCTATGGTGTTGGGT
  TCTGTATTGTTATTATGTATTCTGTTTACATGTTTCTGTGTCACTGCTAAGAGAACAGTCAAGAAATGTT
  TTCCAGAAGACATAGCCCAGCAAAATTTAATTGTATCAAATACTGAAGGACCTGGAGAAGAAGTAACGGA
  AGCAAATATTAGACTCCCCATGCAGACATCCAACATTTGTGACACAAGCATGTCTGTTGGTACTGTTGGT
  GGCCAGGGAATCAAAACACAGCAAAGTTTTGAGATGGTCAAAGGAGGCTACACTTTGGATTCCAACAAAG
  GAGGTGGACATCAGACCTTGGAGTCCGTCAAGGGAGTGGGGCAGGGAGATACTGGCAGATATGCGTACAC
  GGACTGGCAGAGTTTCACCCAACCTCGGCTTGGCGAAAAGGTGTATTTGTGTGGACAAGATGAGGAGCAT
  AAACATTGTGAAGACTACGTTTGTTCGTATAACTATGAAGGCAAAGGTTCTCTGGCCGGCTCAGTAGGTT
  GCTGCAGCGATCGGCAGGAAGAAGAGGGACTGGAGTTTCTAGATCACCTGGAACCCAAATTTAGGACATT
  AGCAAAGACATGCATCAAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214491 representing NM_024421
  Red=Cloning site Green=Tags(s)
  
  MALASAAPGSIFCKQLLFSLLVLTLLCDACQKVYLRVPSHLQAETLVGKVNLEECLKSASLIRSSDPAFR
  ILEDGSIYTTHDLILSSERKSFSIFLSDGQRREQQEIKVVLSARENKSPKKRHTKDTALKRSKRRWAPIP
  ASLMENSLGPFPQHVQQIQSDAAQNYTIFYSISGPGVDKEPFNLFYIEKDTGDIFCTRSIDREKYEQFAL
  YGYATTADGYAPEYPLPLIIKIEDDNDNAPYFEHRVTIFTVPENCRSGTSVGKVTATDLDEPDTLHTRLK
  YKILQQIPDHPKHFSIHPDTGVITTTTPFLDREKCDTYQLIMEVRDMGGQPFGLFNTGTITISLEDENDN
  PPSFTETSYVTEVEENRIDVEILRMKVQDQDLPNTPHSKAVYKILQGNENGNFIISTDPNTNEGVLCVVK
  PLNYEVNRQVILQVGVINEAQFSKAASSQTPTMCTTTVTVKIIDSDEGPECHPPVKVIQSQDGFPAGQEL
  LGYKALDPEISSGEGLRYQKLGDEDNWFEINQHTGDLRTLKVLDRESKFVKNNQYNISVVAVDAVGRSCT
  GTLVVHLDDYNDHAPQIDKEVTICQNNEDFAVLKPVDPDGPENGPPFQFFLDNSASKNWNIEEKDGKTAI
  LRQRQNLDYNYYSVPIQIKDRHGLVATHMLTVRVCDCSTPSECRMKDKSTRDVRPNVILGRWAILAMVLG
  SVLLLCILFTCFCVTAKRTVKKCFPEDIAQQNLIVSNTEGPGEEVTEANIRLPMQTSNICDTSMSVGTVG
  GQGIKTQQSFEMVKGGYTLDSNKGGGHQTLESVKGVGQGDTGRYAYTDWQSFTQPRLGEKVYLCGQDEEH
  KHCEDYVCSYNYEGKGSLAGSVGCCSDRQEEEGLEFLDHLEPKFRTLAKTCIKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024421
• ORF Size: 2682 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024421.2, NP_077739.1
• RefSeq Size: 4225 bp
• RefSeq ORF: 2685 bp
• Locus ID: 1823
• UniProt ID: Q08554
• Cytogenetics: 18q12.1
• Protein Families: Transmembrane
• MW: 96.9 kDa
• Gene Summary: The protein encoded by this gene is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. A subtype of IgA pemphigus, a life-threatening autoimmune disease, is characterized by the presence of autoantibodies that target the encoded protein. The desmosomal family members are arranged in two clusters on chromosome 18. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature protein. [provided by RefSeq, Nov 2015]