LSS (NM_001001438) Human Tagged ORF Clone

CAT#: RC214463

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LSS (Myc-DDK-tagged)-Human lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) (LSS), transcript variant 2



  "NM_001001438" in other vectors (4)

Reconstitution Protocol

USD 670.00

In Stock*

Size
    • 10 ug

Product images

Frequently bought together (4)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 30 ul

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pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 650.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol LSS
Synonyms APMR4; CTRCT44; HYPT14; OSC
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC214463 representing NM_001001438
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGGAGGGCACGTGTCTGCGGCGCCGAGGGGGCCCCTACAAGACCGAGCCCGCCACCGACCTCGGCC
GCTGGCGACTCAACTGCGAGAGGGGCCGGCAGACGTGGACCTACCTGCAGGACGAGCGCGCCGGCCGCGA
GCAGACCGGCCTGGAAGCCTACGCCCTGGGGCTGGACACCAAGAATTACTTTAAGGACTTGCCCAAAGCC
CACACCGCCTTTGAGGGGGCTCTGAACGGGATGACATTTTACGTGGGGCTGCAGGCTGAGGATGGGCACT
GGACGGGTGATTATGGTGGCCCACTTTTCCTCCTGCCAGGCCTCCTGATCACTTGCCACGTGGCACGCAT
CCCTCTGCCAGCCGGATACAGAGAAGAGATTGTGCGGTACCTGCGGTCAGTGCAGCTCCCTGACGGTGGC
TGGGGCCTGCACATTGAGGATAAGTCCACCGTGTTTGGGACTGCGCTCAACTATGTGTCTCTCAGAATTC
TGGGTGTTGGGCCTGACGATCCTGACCTGGTACGAGCCCGGAACATTCTTCACAAGAAAGGTGGTGCTGT
GGCCATCCCCTCCTGGGGGAAGTTCTGGCTGGCTGTCCTGAATGTTTACAGCTGGGAAGGCCTCAATACC
CTGTTCCCAGAGATGTGGCTGTTTCCTGACTGGGCACCGGCACACCCCTCCACACTCTGGTGCCACTGCC
GGCAGGTGTACCTGCCCATGAGCTACTGCTACGCCGTTCGGCTGAGTGCCGCGGAAGACCCGCTGGTCCA
GAGCCTCCGCCAGGAGCTCTATGTGGAGGACTTCGCCAGCATTGACTGGCTGGCGCAGAGGAACAACGTG
GCCCCCGACGAGCTGTACACGCCGCACAGCTGGCTGCTCCGCGTGGTATATGCGCTCCTCAACCTGTATG
AGCACCACCACAGTGCCCACCTGCGGCAGCGGGCCGTGCAGAAGCTGTATGAACACATTGTGGCCGACGA
CCGATTCACCAAGAGCATCAGCATCGGCCCGATCTCGAAAACCATCAACATGCTTGTGCGCTGGTATGTG
GACGGGCCCGCCTCCACTGCCTTCCAGGAGCATGTCTCCAGAATCCCGGACTATCTCTGGATGGGCCTTG
ACGGCATGAAAATGCAGGGCACCAACGGCTCACAGATCTGGGACACCGCATTCGCCATCCAGGCTCTGCT
TGAGGCGGGCGGGCACCACAGGCCCGAGTTTTCGTCCTGCCTGCAGAAGGCTCATGAGTTCCTGAGGCTC
TCACAGGTCCCAGATAACCCTCCCGACTACCAGAAGTACTACCGCCAGATGCGCAAGGGTGGCTTCTCCT
TCAGTACGCTGGACTGCGGCTGGATCGTTTCTGACTGCACGGCTGAGGCCTTGAAGGCTGTGCTGCTCCT
GCAGGAGAAGTGTCCCCATGTCACCGAGCACATCCCCAGAGAACGGCTCTGCGATGCTGTGGCTGTGCTG
CTGAACATGAGAAATCCAGATGGAGGGTTCGCCACCTATGAGACCAAGCGTGGGGGGCACTTGCTGGAGC
TGCTGAACCCCTCGGAGGTCTTCGGGGACATCATGATTGACTACACCTATGTGGAGTGCACCTCAGCCGT
GATGCAGGCGCTTAAGTATTTCCACAAGCGTTTCCCGGAGCACAGGGCAGCGGAGATCCGGGAGACCCTC
ACGCAGGGCTTAGAGTTCTGTCGGCGGCAGCAGAGGGCCGATGGCTCCTGGGAAGGCTCCTGGGGAGTTT
GCTTCACCTACGGCACCTGGTTTGGCCTGGAGGCCTTCGCCTGTATGGGGCAGACCTACCGAGATGGGAC
TGCCTGTGCAGAGGTCTCCCGGGCCTGTGACTTCCTGCTGTCCCGGCAGATGGCAGACGGAGGCTGGGGG
GAGGACTTTGAGTCCTGCGAGGAGCGGCGTTATTTGCAGAGTGCCCAGTCCCAGATCCATAACACATGCT
GGGCCATGATGGGGCTGATGGCCGTTCGGCATCCTGACATCGAGGCCCAGGAGAGAGGAGTCCGGTGTCT
ACTTGAGAAACAGCTCCCCAATGGCGACTGGCCGCAGGAAAACATTGCTGGGGTCTTCAACAAGTCCTGT
GCCATCTCCTACACGAGCTACAGGAACATCTTCCCCATCTGGGCCCTCGGCCGCTTCTCCCAGCTGTACC
CTGAGAGAGCCCTTGCTGGCCACCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC214463 representing NM_001001438
Red=Cloning site Green=Tags(s)

MTEGTCLRRRGGPYKTEPATDLGRWRLNCERGRQTWTYLQDERAGREQTGLEAYALGLDTKNYFKDLPKA
HTAFEGALNGMTFYVGLQAEDGHWTGDYGGPLFLLPGLLITCHVARIPLPAGYREEIVRYLRSVQLPDGG
WGLHIEDKSTVFGTALNYVSLRILGVGPDDPDLVRARNILHKKGGAVAIPSWGKFWLAVLNVYSWEGLNT
LFPEMWLFPDWAPAHPSTLWCHCRQVYLPMSYCYAVRLSAAEDPLVQSLRQELYVEDFASIDWLAQRNNV
APDELYTPHSWLLRVVYALLNLYEHHHSAHLRQRAVQKLYEHIVADDRFTKSISIGPISKTINMLVRWYV
DGPASTAFQEHVSRIPDYLWMGLDGMKMQGTNGSQIWDTAFAIQALLEAGGHHRPEFSSCLQKAHEFLRL
SQVPDNPPDYQKYYRQMRKGGFSFSTLDCGWIVSDCTAEALKAVLLLQEKCPHVTEHIPRERLCDAVAVL
LNMRNPDGGFATYETKRGGHLLELLNPSEVFGDIMIDYTYVECTSAVMQALKYFHKRFPEHRAAEIRETL
TQGLEFCRRQQRADGSWEGSWGVCFTYGTWFGLEAFACMGQTYRDGTACAEVSRACDFLLSRQMADGGWG
EDFESCEERRYLQSAQSQIHNTCWAMMGLMAVRHPDIEAQERGVRCLLEKQLPNGDWPQENIAGVFNKSC
AISYTSYRNIFPIWALGRFSQLYPERALAGHP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001001438
ORF Size 2196 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001001438.2, NP_001001438.1
RefSeq Size 2658 bp
RefSeq ORF 2199 bp
Locus ID 4047
UniProt ID P48449
Cytogenetics 21q22.3
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Steroid biosynthesis
MW 83.1 kDa
Gene Summary The protein encoded by this gene catalyzes the conversion of (S)-2,3 oxidosqualene to lanosterol. The encoded protein is a member of the terpene cyclase/mutase family and catalyzes the first step in the biosynthesis of cholesterol, steroid hormones, and vitamin D. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Feb 2009]

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