NSUN2 (NM_017755) Human Tagged ORF Clone

CAT#: RC214459

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NSUN2 (Myc-DDK-tagged)-Human NOP2/Sun domain family, member 2 (NSUN2), transcript variant 1



Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 691.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol NSUN2
Synonyms MISU; MRT5; SAKI; TRM4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC214459 representing NM_017755
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCGGCGGTCGCGGGGTCGGCGGCTCCAGCAACAGCAGCGGCCGGAGGACGCGGAGGATGGCGCCG
AGGGTGGTGGAAAGCGCGGCGAGGCGGGCTGGGAAGGAGGCTACCCCGAGATCGTCAAGGAGAACAAGCT
GTTCGAGCACTACTACCAGGAGCTCAAGATCGTGCCCGAGGGCGAGTGGGGCCAGTTCATGGACGCTCTC
AGGGAGCCGCTCCCGGCCACTTTAAGAATTACTGGTTACAAAAGCCACGCAAAAGAGATTCTCCATTGCT
TAAAGAACAAATATTTTAAGGAATTGGAGGACCTGGAGGTGGACGGTCAGAAAGTTGAAGTTCCACAGCC
ACTGAGTTGGTATCCTGAAGAACTTGCCTGGCACACAAATTTAAGTCGAAAAATCTTGAGAAAATCGCCA
CACTTGGAAAAGTTTCATCAGTTTCTAGTTAGTGAAACAGAATCTGGAAATATTAGTCGTCAAGAAGCTG
TTAGCATGATCCCACCACTGCTCCTCAACGTGCGGCCTCATCATAAGATCTTAGATATGTGTGCAGCACC
TGGCTCAAAGACCACACAGTTAATTGAAATGCTACATGCCGACATGAATGTCCCCTTTCCAGAGGGATTT
GTTATTGCGAATGATGTGGACAACAAGCGCTGCTACCTGCTCGTCCATCAAGCCAAGAGGCTGAGCAGCC
CCTGCATCATGGTGGTCAACCATGATGCCTCCAGCATACCCAGGCTCCAGATAGATGTGGACGGCAGGAA
AGAGATCCTCTTCTATGATCGAATTTTATGTGATGTCCCTTGCAGTGGAGACGGCACTATGAGAAAAAAC
ATTGATGTTTGGAAAAAGTGGACCACCTTAAATAGCTTGCAGCTACATGGCTTACAGCTGCGGATTGCAA
CACGCGGGGCTGAACAGCTGGCTGAAGGTGGAAGGATGGTGTATTCCACGTGTTCACTAAACCCTATTGA
GGATGAAGCAGTCATAGCATCTTTACTGGAAAAAAGTGAAGGTGCTTTGGAGCTTGCTGATGTGTCTAAT
GAACTGCCAGGGCTGAAGTGGATGCCTGGAATCACACAGTGGAAGGTAATGACGAAAGATGGGCAGTGGT
TTACAGACTGGGACGCTGTTCCTCACAGCAGACACACCCAGATCCGACCTACCATGTTCCCTCCGAAGGA
CCCAGAAAAGCTGCAGGCCATGCACCTGGAGCGATGCCTTAGGATATTACCCCATCATCAGAATACTGGA
GGGTTTTTTGTGGCAGTATTGGTGAAAAAATCTTCAATGCCGTGGAATAAACGTCAGCCAAAGCTTCAGG
GTAAATCTGCAGAGACCAGAGAAAGCACACAGCTGAGCCCTGCAGATCTCACAGAAGGGAAACCCACAGA
TCCCTCTAAGCTGGAAAGTCCGTCATTCACAGGAACTGGTGACACAGAAATAGCTCATGCAACTGAGGAT
TTAGAGAATAATGGCAGTAAGAAAGATGGCGTGTGTGGTCCTCCTCCATCAAAGAAAATGAAGTTATTTG
GATTTAAAGAAGATCCATTTGTATTTATTCCTGAAGATGACCCATTATTTCCACCTATTGAGAAATTTTA
TGCTTTGGATCCTTCATTCCCAAGGATGAATTTGTTAACTCGGACTACAGAAGGGAAGAAAAGGCAGCTC
TACATGGTTTCTAAGGAGTTGCGGAATGTGCTGCTGAATAACAGTGAGAAGATGAAGGTTATTAACACGG
GGATCAAAGTCTGGTGTAGAAATAACAGCGGTGAAGAGTTTGACTGTGCTTTCCGGCTGGCACAGGAGGG
AATATATACATTGTATCCATTTATTAACTCAAGAATTATTACTGTATCAATGGAAGATGTTAAGATACTG
TTGACCCAGGAAAATCCCTTTTTTAGAAAACTCAGCAGTGAGACCTACAGTCAAGCAAAGGACCTGGCAA
AGGGAAGCATCGTGCTGAAGTATGAACCAGATTCTGCGAATCCAGACGCTCTGCAGTGTCCCATCGTCTT
ATGCGGATGGCGGGGAAAGGCCTCCATTCGAACTTTTGTGCCCAAGAATGAACGGCTTCATTATCTCAGG
ATGATGGGGCTGGAGGTATTGGGAGAAAAGAAGAAGGAAGGGGTTATCCTCACAAATGAGAGTGCAGCCA
GCACCGGACAGCCAGACAATGACGTGACTGAGGGACAGAGAGCAGGAGAGCCCAACAGCCCAGATGCAGA
AGAGGCCAACAGTCCAGACGTGACAGCAGGCTGTGACCCGGCGGGGGTCCATCCACCCCGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC214459 representing NM_017755
Red=Cloning site Green=Tags(s)

MGRRSRGRRLQQQQRPEDAEDGAEGGGKRGEAGWEGGYPEIVKENKLFEHYYQELKIVPEGEWGQFMDAL
REPLPATLRITGYKSHAKEILHCLKNKYFKELEDLEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSP
HLEKFHQFLVSETESGNISRQEAVSMIPPLLLNVRPHHKILDMCAAPGSKTTQLIEMLHADMNVPFPEGF
VIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASSIPRLQIDVDGRKEILFYDRILCDVPCSGDGTMRKN
IDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSN
ELPGLKWMPGITQWKVMTKDGQWFTDWDAVPHSRHTQIRPTMFPPKDPEKLQAMHLERCLRILPHHQNTG
GFFVAVLVKKSSMPWNKRQPKLQGKSAETRESTQLSPADLTEGKPTDPSKLESPSFTGTGDTEIAHATED
LENNGSKKDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQL
YMVSKELRNVLLNNSEKMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKIL
LTQENPFFRKLSSETYSQAKDLAKGSIVLKYEPDSANPDALQCPIVLCGWRGKASIRTFVPKNERLHYLR
MMGLEVLGEKKKEGVILTNESAASTGQPDNDVTEGQRAGEPNSPDAEEANSPDVTAGCDPAGVHPPR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_017755
ORF Size 2301 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_017755.6
RefSeq Size 3089 bp
RefSeq ORF 2304 bp
Locus ID 54888
UniProt ID Q08J23
Cytogenetics 5p15.31
Domains Nol1_Nop2_Sun
MW 86.3 kDa
Gene Summary This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Mar 2011]
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