TMEM150A (NM_153342) Human Tagged ORF Clone
CAT#: RC214444
TMEM150A (Myc-DDK-tagged)-Human transmembrane protein 150A (TMEM150A), transcript variant 2
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_153342" in other vectors (4)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | TMEM150A |
Synonyms | TM6P1; TMEM150; TTN1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC214444 representing NM_153342
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATGCCCTGTGGAGAACTGGTCCTACAACGAGTCCTGCCCTCCTGACCCTGCTGAGCAAGGGGGTCC CAAGACCTGCTGCACCCTGGACGATGTCCCCCTCATCAGTGGCCCTGATCTGCCTCCTGCGCTACGGGCA GCTCCTGGAGCAGAGTCGGCACTCTTGGGTTAACACCACGGCACTCATCACAGGCTGCACCAACGCTGCG GGCCTCTTGGTGGTTGGCAACTTTCAGGTGGATCATGCCAGGTCTCTGCACTACGTTGGAGCTGGCGTGG CCTTCCCTGCGGGGCTGCTCTTTGTTTGCCTGCACTGTGCTCTCTCCTACCAAGGGGCCACCGCCCCGCT GGACCTGGCTGTGGCCTATCTGCGAAGTGTGCTGGCTGTCATCGCCTTTATCACCCTGGTCCTCAGTGGA GTCTTCTTTGTCCATGAGAGTTCTCAGCTGCAACATGGGGCAGCCCTGTGTGAGTGGGTGTGTGTCATCG ATATCCCCATTTTCTATGGCACCTTCAGCTACGAGTTTGGGGCAGTCTCCTCAGACACACTGGTGGCTGC ACTGCAGCCTACCCCTGGCCGGGCCTGCAAGTCCTCCGGGAGCAGCAGCACCTCCACCCACCTCAACTGT GCCCCCGAGAGCATCGCTATGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214444 representing NM_153342
Red=Cloning site Green=Tags(s) MYALWRTGPTTSPALLTLLSKGVPRPAAPWTMSPSSVALICLLRYGQLLEQSRHSWVNTTALITGCTNAA GLLVVGNFQVDHARSLHYVGAGVAFPAGLLFVCLHCALSYQGATAPLDLAVAYLRSVLAVIAFITLVLSG VFFVHESSQLQHGAALCEWVCVIDIPIFYGTFSYEFGAVSSDTLVAALQPTPGRACKSSGSSSTSTHLNC APESIAMI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_153342 |
ORF Size | 654 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_153342.2 |
RefSeq Size | 1486 bp |
RefSeq ORF | 657 bp |
Locus ID | 129303 |
Cytogenetics | 2p11.2 |
Protein Families | Transmembrane |
MW | 23.5 kDa |
Gene Summary | Regulates localization of phosphatidylinositol 4-kinase (PI4K) to the plasma membrane, possibly by reducing the association of TTC7 (TTC7A or TTC7B) with the PI4K complex (PubMed:25608530). Acts as a regulator of phosphatidylinositol 4-phosphate (PtdIns(4)P) synthesis (PubMed:25608530). May also play a role in fasting-induced catabolism (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC214444L3 | Lenti ORF clone of Human transmembrane protein 150A (TMEM150A), transcript variant 2, Myc-DDK-tagged |
USD 600.00 |
|
RC214444L4 | Lenti ORF clone of Human transmembrane protein 150A (TMEM150A), transcript variant 2, mGFP tagged |
USD 600.00 |
|
RG214444 | TMEM150A (tGFP-tagged) - Human transmembrane protein 150A (TMEM150A), transcript variant 2 |
USD 500.00 |
|
SC100343 | TMEM150A (untagged)-Human transmembrane protein 150A (TMEM150A), transcript variant 2 |
USD 300.00 |
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