ADCY7 (NM_001114) Human Tagged ORF Clone

CAT#: RC214351

ADCY7 (Myc-DDK-tagged)-Human adenylate cyclase 7 (ADCY7)

Product Images

Circular map for RC214351

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADCY7
• Synonyms: AC7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214351 representing NM_001114
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGCCAAGGGGCGCTACTTCCTCAACGAGGGCGAGGAGGGCCCTGACCAAGATGCGCTCTACGAGA
  AGTACCAGCTCACCAGCCAGCATGGGCCGCTGCTGCTCACGCTCCTGCTGGTGGCCGCCACTGCCTGCGT
  GGCCCTCATCATCATTGCCTTCAGCCAGGGGGACCCCTCCAGACACCAGGCCATTCTGGGCATGGCGTTC
  CTGGTGCTGGCGGTGTTTGCGGCCCTCTCTGTGCTGATGTACGTCGAGTGTCTCCTGCGGCGCTGGCTCA
  GGGCCTTGGCGCTGCTCACCTGGGCCTGCTTGGTGGCGCTGGGCTATGTGCTGGTGTTCGACGCATGGAC
  AAAGGCGGCCTGTGCGTGGGAGCAGGTGCCCTTCTTCCTGTTCATTGTCTTCGTGGTGTACACACTACTG
  CCCTTCAGCATGCGGGGCGCTGTCGCCGTTGGGGCCGTCTCCACTGCCTCCCACCTCCTGGTGCTCGGTT
  CTTTGATGGGAGGCTTCACGACACCCAGTGTCCGGGTGGGGCTGCAGCTGCTGGCCAACGCAGTCATCTT
  CCTGTGTGGGAACCTGACAGGCGCCTTCCACAAGCACCAAATGCAGGATGCGTCCCGGGACCTCTTCACC
  TACACTGTGAAGTGCATCCAGATCCGCCGGAAGCTGCGCATCGAGAAGCGCCAGCAGGAGAACCTGCTGC
  TGTCAGTGCTTCCGGCCCACATCTCCATGGGCATGAAGCTGGCCATCATCGAACGGCTCAAGGAGCATGG
  TGACCGTCGCTGCATGCCTGACAACAACTTCCACAGCCTCTACGTCAAGAGGCACCAGAATGTCAGCATC
  CTCTATGCGGACATCGTGGGCTTCACGCAGCTGGCCAGCGACTGTTCTCCCAAGGAGCTGGTGGTGGTGC
  TGAATGAGCTCTTTGGCAAGTTCGACCAGATCGCCAAGGCCAACGAGTGCATGCGAATCAAGATCCTCGG
  CGACTGCTACTACTGTGTATCGGGCCTGCCCGTGTCGCTGCCTACCCACGCCCGGAACTGCGTGAAGATG
  GGGCTGGACATGTGCCAGGCCATCAAGCAGGTGCGGGAGGCCACGGGCGTGGACATCAACATGCGTGTGG
  GCATACACTCGGGGAATGTGCTGTGCGGGGTCATCGGGCTGCGCAAGTGGCAGTATGACGTGTGGTCCCA
  CGACGTGTCCCTGGCCAACCGGATGGAGGCAGCCGGAGTACCCGGCCGGGTGCACATCACGGAGGCCACG
  CTAAAGCACCTGGACAAGGCGTACGAGGTGGAGGATGGGCACGGGCAGCAGCGGGACCCCTACCTCAAGG
  AGATGAACATCCGCACCTACCTGGTCATCGACCCCCGGAGCCAGCAGCCACCCCCGCCCAGCCAACACCT
  CCCCAGGCCCAAGGGGGACGCGGCCCTGAAGATGCGGGCGTCAGTGCGCATGACCCGGTACCTCGAGTCC
  TGGGGGGCGGCACGGCCCTTTGCACATCTCAACCACCGTGAGAGCGTGAGCAGTGGTGAGACCCACGTCC
  CCAACGGGCGGAGGCCTAAGAGCGTTCCCCAGCGCCACCGCCGGACCCCAGACAGAAGCATGTCCCCCAA
  GGGGCGGTCGGAGGATGACTCGTACGATGACGAGATGCTGTCAGCCATTGAGGGGCTCAGCTCCACGAGG
  CCCTGCTGCTCCAAGTCCGATGACTTCTACACCTTTGGGTCCATCTTCCTGGAGAAGGGCTTTGAGCGCG
  AGTACCGCCTGGCACCCATCCCCCGGGCCCGCCACGACTTTGCCTGCGCCAGCCTGATCTTCGTCTGCAT
  CCTGCTCGTCCATGTCCTGCTCATGCCCAGGACGGCGGCACTGGGTGTGTCCTTCGGGCTGGTGGCCTGT
  GTACTGGGGCTGGTGCTGGGCCTGTGCTTTGCCACCAAGTTCTCGAGGTGCTGCCCAGCTCGGGGGACGC
  TCTGCACTATCTCTGAGAGGGTGGAGACACAGCCCCTGCTGAGGCTGACCCTGGCCGTCCTGACCATCGG
  CAGCCTGCTCACTGTGGCCATCATCAACCTGCCCCTGATGCCTTTCCAAGTTCCAGAGCTGCCTGTTGGC
  AATGAGACAGGCCTACTGGCCGCGAGCAGCAAGACAAGAGCCCTGTGTGAGCCCCTCCCGTACTACACCT
  GCAGCTGTGTCCTGGGCTTCATCGCCTGCTCGGTCTTCCTGAGGATGAGCCTGGAGCCAAAGGTTGTGCT
  GCTGACAGTGGCCCTGGTGGCCTACCTGGTGCTCTTCAACCTCTCCCCATGCTGGCAGTGGGACTGCTGC
  GGCCAAGGCCTGGGCAACCTCACCAAGCCCAACGGCACCACCAGTGGCACCCCTAGCTGTTCCTGGAAGG
  ACCTGAAGACCATGACCAATTTCTACCTGGTCCTGTTCTACATCACCCTGCTTACACTCTCCAGACAGAT
  TGACTATTACTGCCGCTTGGACTGCCTATGGAAGAAGAAGTTCAAGAAGGAGCACGAGGAGTTTGAGACC
  ATGGAGAACGTGAACCGCCTTCTTCTGGAGAACGTCCTGCCAGCCCACGTGGCTGCCCACTTTATCGGTG
  ACAAGTTAAACGAGGACTGGTACCATCAGTCCTATGACTGCGTCTGTGTCATGTTTGCCTCCGTGCCGGA
  CTTCAAAGTGTTCTACACAGAGTGCGATGTCAACAAAGAAGGGCTGGAGTGCCTACGCCTGCTCAATGAG
  ATCATTGCCGACTTCGACGAGCTCCTACTGAAGCCCAAGTTCAGCGGCGTGGAGAAGATCAAGACCATCG
  GCAGCACGTACATGGCAGCTGCAGGGCTCAGCGTCGCCTCAGGGCACGAGAACCAGGAGCTGGAGCGGCA
  GCATGCCCACATTGGTGTCATGGTGGAGTTCAGCATCGCCCTGATGAGTAAGCTGGACGGCATCAACAGG
  CACTCCTTCAACTCCTTCCGCCTCCGCGTCGGCATAAACCATGGGCCTGTGATTGCTGGAGTGATTGGGG
  CCCGAAAACCTCAGTATGACATCTGGGGAAACACTGTCAATGTGGCCAGCCGAATGGAAAGCACTGGAGA
  ACTTGGGAAAATCCAGGTTACCGAGGAGACCTGCACCATCCTCCAGGGCCTCGGGTACTCTTGTGAATGC
  CGTGGCCTGATCAACGTCAAAGGCAAAGGCGAGCTGAGGACTTACTTTGTCTGTACGGACACTGCCAAGT
  TTCAGGGGCTGGGGCTGAAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214351 representing NM_001114
  Red=Cloning site Green=Tags(s)
  
  MPAKGRYFLNEGEEGPDQDALYEKYQLTSQHGPLLLTLLLVAATACVALIIIAFSQGDPSRHQAILGMAF
  LVLAVFAALSVLMYVECLLRRWLRALALLTWACLVALGYVLVFDAWTKAACAWEQVPFFLFIVFVVYTLL
  PFSMRGAVAVGAVSTASHLLVLGSLMGGFTTPSVRVGLQLLANAVIFLCGNLTGAFHKHQMQDASRDLFT
  YTVKCIQIRRKLRIEKRQQENLLLSVLPAHISMGMKLAIIERLKEHGDRRCMPDNNFHSLYVKRHQNVSI
  LYADIVGFTQLASDCSPKELVVVLNELFGKFDQIAKANECMRIKILGDCYYCVSGLPVSLPTHARNCVKM
  GLDMCQAIKQVREATGVDINMRVGIHSGNVLCGVIGLRKWQYDVWSHDVSLANRMEAAGVPGRVHITEAT
  LKHLDKAYEVEDGHGQQRDPYLKEMNIRTYLVIDPRSQQPPPPSQHLPRPKGDAALKMRASVRMTRYLES
  WGAARPFAHLNHRESVSSGETHVPNGRRPKSVPQRHRRTPDRSMSPKGRSEDDSYDDEMLSAIEGLSSTR
  PCCSKSDDFYTFGSIFLEKGFEREYRLAPIPRARHDFACASLIFVCILLVHVLLMPRTAALGVSFGLVAC
  VLGLVLGLCFATKFSRCCPARGTLCTISERVETQPLLRLTLAVLTIGSLLTVAIINLPLMPFQVPELPVG
  NETGLLAASSKTRALCEPLPYYTCSCVLGFIACSVFLRMSLEPKVVLLTVALVAYLVLFNLSPCWQWDCC
  GQGLGNLTKPNGTTSGTPSCSWKDLKTMTNFYLVLFYITLLTLSRQIDYYCRLDCLWKKKFKKEHEEFET
  MENVNRLLLENVLPAHVAAHFIGDKLNEDWYHQSYDCVCVMFASVPDFKVFYTECDVNKEGLECLRLLNE
  IIADFDELLLKPKFSGVEKIKTIGSTYMAAAGLSVASGHENQELERQHAHIGVMVEFSIALMSKLDGINR
  HSFNSFRLRVGINHGPVIAGVIGARKPQYDIWGNTVNVASRMESTGELGKIQVTEETCTILQGLGYSCEC
  RGLINVKGKGELRTYFVCTDTAKFQGLGLN
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001114
• ORF Size: 3240 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001114.4
• RefSeq Size: 6157 bp
• RefSeq ORF: 3243 bp
• Locus ID: 113
• UniProt ID: P51828
• Cytogenetics: 16q12.1
• Domains: CYCc
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Melanogenesis, Oocyte meiosis, Progesterone-mediated oocyte maturation, Purine metabolism, Vascular smooth muscle contraction
• MW: 120.1 kDa
• Gene Summary: This gene encodes a membrane-bound adenylate cyclase that catalyses the formation of cyclic AMP from ATP and is inhibitable by calcium. The product of this gene is a member of the adenylyl cyclase class-4/guanylyl cyclase enzyme family that is characterized by the presence of twelve membrane-spanning domains in its sequences. Several transcript variants have been observed for this gene, but the full-length natures of only two have been determined so far. [provided by RefSeq, Oct 2013]