TM6SF2 (NM_001001524) Human Tagged ORF Clone

CAT#: RC214298

TM6SF2 (Myc-DDK-tagged)-Human transmembrane 6 superfamily member 2 (TM6SF2)

Product Images

Circular map for RC214298

• 

  Western blot validation of overexpression lysate (Cat# LY424371) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214298 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TM6SF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214298 representing NM_001001524
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACATCCCGCCGCTGGCCGGCAAGATCGCGGCGCTGTCGCTGAGCGCCCTCCCGGTGTCCTACGCGC
  TCAACCACGTCTCGGCGCTCTCGCACCCCCTGTGGGTGGCATTGATGAGCGCCCTAATCCTGGGTCTGCT
  TTTCGTGGCGGTCTACAGCTTGTCCCATGGCGAGGTCTCCTATGACCCACTCTATGCTGTCTTCGCTGTC
  TTCGCCTTCACCTCGGTTGTGGACCTCATCATCGCTCTTCAGGAAGACAGCTATGTGGTGGGCTTCATGG
  AGTTCTACACCAAGGAGGGAGAGCCATACCTGCGCACAGCGCACGGAGTCTTCATCTGCTACTGGGATGG
  CACTGTTCACTACCTCCTCTACCTGGCCATGGCCGGCGCCATCTGCAGAAGGAAGAGATACCGGAATTTT
  GGACTCTACTGGCTGGGTTCCTTCGCCATGAGCATCCTGGTGTTCCTTACAGGAAACATTCTTGGCAAAT
  ACAGCTCCGAGATCAGGCCTGCCTTCTTCCTCACCATCCCCTACCTGCTGGTGCCATGCTGGGCTGGCAT
  GAAGGTCTTCAGCCAGCCCCGGGCGCTAACCCGCTGCACCGCCAACATGGTGCAAGAGGAACAAAGAAAG
  GGACTCCTGCAGCGTCCGGCTGACCTGGCCCTTGTCATATATCTCATCCTTGCTGGCTTCTTCACTCTGT
  TCCGGGGCCTGGTGGTGCTTGATTGCCCCACAGATGCCTGCTTTGTCTATATCTACCAGTATGAGCCATA
  CCTGCGGGACCCTGTGGCCTACCCTAAGGTGCAGATGCTGATGTACATGTTTTATGTCCTGCCTTTCTGC
  GGCCTGGCTGCCTATGCTCTCACCTTCCCTGGTTGCTCCTGGCTTCCAGACTGGGCCTTGGTGTTTGCTG
  GAGGCATCGGCCAGGCACAGTTCTCGCACATGGGGGCTTCCATGCACCTGCGCACACCCTTCACCTACCG
  TGTGCCTGAGGACACCTGGGGCTGCTTCTTCGTGTGCAATCTGCTGTATGCGCTGGGCCCCCACCTGCTG
  GCCTACCGTTGCCTTCAGTGGCCCGCATTCTTCCACCAGCCACCACCCTCCGACCCCCTAGCCCTCCACA
  AGAAGCAGCAT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214298 representing NM_001001524
  Red=Cloning site Green=Tags(s)
  
  MDIPPLAGKIAALSLSALPVSYALNHVSALSHPLWVALMSALILGLLFVAVYSLSHGEVSYDPLYAVFAV
  FAFTSVVDLIIALQEDSYVVGFMEFYTKEGEPYLRTAHGVFICYWDGTVHYLLYLAMAGAICRRKRYRNF
  GLYWLGSFAMSILVFLTGNILGKYSSEIRPAFFLTIPYLLVPCWAGMKVFSQPRALTRCTANMVQEEQRK
  GLLQRPADLALVIYLILAGFFTLFRGLVVLDCPTDACFVYIYQYEPYLRDPVAYPKVQMLMYMFYVLPFC
  GLAAYALTFPGCSWLPDWALVFAGGIGQAQFSHMGASMHLRTPFTYRVPEDTWGCFFVCNLLYALGPHLL
  AYRCLQWPAFFHQPPPSDPLALHKKQH
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001524
• ORF Size: 1131 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001524.3
• RefSeq Size: 1483 bp
• RefSeq ORF: 1134 bp
• Locus ID: 53345
• UniProt ID: Q9BZW4
• Cytogenetics: 19p13.11
• Protein Families: Transmembrane
• MW: 42.4 kDa
• Gene Summary: Regulator of liver fat metabolism influencing triglyceride secretion and hepatic lipid droplet content (PubMed:24531328, PubMed:24927523). May function as sterol isomerase (PubMed:25566323).[UniProtKB/Swiss-Prot Function]