ZFX (NM_003410) Human Tagged ORF Clone

CAT#: RC214045

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ZFX (Myc-DDK-tagged)-Human zinc finger protein, X-linked (ZFX), transcript variant 1



  "NM_003410" in other vectors (6)

Reconstitution Protocol

USD 1,105.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ZFX
Synonyms ZNF926
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC214045 representing NM_003410
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGAAGATGGGCTTGAATTACAACAAGAGCCAAACTCATTTTTTGATGCAACAGGAGCTGATGGTA
CACACATGGATGGTGATCAAATTGTTGTGGAAGTACAAGAAACTGTTTTTGTTTCAGATGTTGTGGATTC
AGACATAACTGTGCATAACTTTGTTCCTGATGACCCAGATTCAGTTGTAATCCAAGATGTTATTGAGGAC
GTTGTTATAGAAGATGTTCAGTGCCCAGATATCATGGAAGAAGCAGATGTGTCTGAAACGGTCATCATTC
CTGAGCAAGTGCTGGACTCAGATGTAACTGAAGAAGTTTCTTTAGCACATTGCACAGTCCCAGATGATGT
TTTAGCTTCTGACATTACTTCAGCCTCAATGTCTATGCCAGAACACGTCTTGACGGGTGATTCTATACAT
GTGTCTGACGTTGGACATGTTGGACATGTTGGACATGTTGAACATGTGGTTCATGATAGTGTAGTGGAAG
CAGAAATTGTCACTGATCCTCTGACTACCGACGTAGTTTCAGAAGAAGTATTGGTAGCAGACTGTGCCTC
TGAAGCAGTCATAGATGCCAATGGGATCCCTGTGGACCAGCAGGATGATGACAAAGGCAACTGTGAGGAC
TACCTTATGATTTCCTTGGATGATGCTGGCAAAATAGAACACGATGGTTCTTCTGGAATGACCATGGACA
CAGAGTCGGAAATTGATCCTTGTAAAGTGGATGGCACTTGCCCTGAGGTCATCAAGGTGTACATTTTTAA
AGCTGACCCTGGAGAAGATGACTTAGGTGGAACTGTAGACATTGTGGAGAGTGAGCCTGAGAATGATCAT
GGAGTTGAACTGCTTGATCAGAACAGCAGTATTCGTGTTCCCAGGGAAAAGATGGTTTATATGACTGTCA
ATGACTCTCAGCCAGAAGATGAAGATTTAAATGTTGCTGAAATCGCTGACGAAGTTTATATGGAAGTGAT
CGTAGGAGAGGAGGATGCTGCAGCAGCAGCGGCAGCCGCCGCCGTGCACGAGCAGCAAATGGATGACAAT
GAAATCAAAACCTTCATGCCGATTGCATGGGCAGCAGCTTATGGTAATAATTCTGATGGAATTGAAAACC
GGAATGGCACTGCAAGTGCCCTCTTGCACATAGATGAGTCTGCTGGCCTCGGCAGACTGGCTAAACAAAA
ACCAAAGAAAAGGAGAAGACCTGATTCCAGGCAGTACCAAACAGCAATAATTATTGGCCCTGATGGACAT
CCTTTGACTGTCTATCCTTGCATGATTTGTGGGAAGAAGTTTAAGTCGAGAGGTTTTTTGAAAAGGCACA
TGAAAAACCATCCCGAACACCTTGCCAAGAAGAAATACCGCTGTACTGACTGTGATTACACTACCAACAA
GAAGATAAGTTTACACAACCACCTGGAGAGCCACAAGCTGACCAGCAAGGCAGAGAAGGCCATTGAATGC
GATGAGTGTGGGAAGCATTTCTCTCATGCAGGGGCTTTGTTTACTCACAAAATGGTGCATAAGGAAAAAG
GAGCCAACAAAATGCACAAGTGTAAATTCTGTGAATACGAGACAGCTGAACAAGGGTTATTGAATCGCCA
CCTCTTGGCAGTCCACAGCAAGAACTTTCCTCATATTTGTGTGGAGTGTGGTAAGGGTTTTCGTCACCCG
TCAGAGCTCAAAAAGCACATGAGAATCCATACTGGGGAGAAGCCGTACCAATGCCAGTACTGCGAATATA
GGTCTGCAGACTCTTCTAACTTGAAAACGCATGTCAAAACTAAGCATAGTAAAGAGATGCCATTCAAGTG
TGACATTTGTCTTCTGACTTTCTCGGATACCAAAGAGGTGCAGCAACATGCTCTTATCCACCAAGAAAGC
AAAACACACCAGTGTTTGCATTGCGACCACAAGAGTTCGAACTCAAGTGATTTGAAACGACACATAATTT
CAGTTCACACGAAAGACTACCCCCATAAGTGTGACATGTGTGATAAAGGCTTTCACAGGCCTTCAGAACT
CAAGAAACACGTGGCTGCCCACAAGGGCAAAAAAATGCACCAGTGTAGACATTGTGACTTTAAGATTGCA
GATCCATTTGTTCTAAGTCGCCATATTCTCTCAGTTCACACAAAGGATCTTCCATTTAGGTGCAAGAGAT
GTAGAAAGGGATTTAGGCAACAGAGTGAGCTTAAAAAGCATATGAAGACACACAGTGGCAGGAAAGTGTA
TCAGTGTGAGTACTGTGAGTATAGCACTACAGATGCCTCAGGCTTTAAACGGCACGTTATTTCCATTCAC
ACGAAAGACTATCCTCACCGGTGTGAGTACTGCAAGAAAGGCTTCCGAAGACCTTCAGAAAAGAACCAGC
ACATAATGCGACATCATAAAGAAGTTGGCCTGCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC214045 representing NM_003410
Red=Cloning site Green=Tags(s)

MDEDGLELQQEPNSFFDATGADGTHMDGDQIVVEVQETVFVSDVVDSDITVHNFVPDDPDSVVIQDVIED
VVIEDVQCPDIMEEADVSETVIIPEQVLDSDVTEEVSLAHCTVPDDVLASDITSASMSMPEHVLTGDSIH
VSDVGHVGHVGHVEHVVHDSVVEAEIVTDPLTTDVVSEEVLVADCASEAVIDANGIPVDQQDDDKGNCED
YLMISLDDAGKIEHDGSSGMTMDTESEIDPCKVDGTCPEVIKVYIFKADPGEDDLGGTVDIVESEPENDH
GVELLDQNSSIRVPREKMVYMTVNDSQPEDEDLNVAEIADEVYMEVIVGEEDAAAAAAAAAVHEQQMDDN
EIKTFMPIAWAAAYGNNSDGIENRNGTASALLHIDESAGLGRLAKQKPKKRRRPDSRQYQTAIIIGPDGH
PLTVYPCMICGKKFKSRGFLKRHMKNHPEHLAKKKYRCTDCDYTTNKKISLHNHLESHKLTSKAEKAIEC
DECGKHFSHAGALFTHKMVHKEKGANKMHKCKFCEYETAEQGLLNRHLLAVHSKNFPHICVECGKGFRHP
SELKKHMRIHTGEKPYQCQYCEYRSADSSNLKTHVKTKHSKEMPFKCDICLLTFSDTKEVQQHALIHQES
KTHQCLHCDHKSSNSSDLKRHIISVHTKDYPHKCDMCDKGFHRPSELKKHVAAHKGKKMHQCRHCDFKIA
DPFVLSRHILSVHTKDLPFRCKRCRKGFRQQSELKKHMKTHSGRKVYQCEYCEYSTTDASGFKRHVISIH
TKDYPHRCEYCKKGFRRPSEKNQHIMRHHKEVGLP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003410
ORF Size 2415 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003410.4
RefSeq Size 5811 bp
RefSeq ORF 2418 bp
Locus ID 7543
UniProt ID P17010
Cytogenetics Xp22.11
Domains Zfx_Zfy_act, zf-C2H2
Protein Families Transcription Factors
MW 90.3 kDa
Gene Summary This gene on the X chromosome is structurally similar to a related gene on the Y chromosome. It encodes a member of the krueppel C2H2-type zinc-finger protein family. The full-length protein contains an acidic transcriptional activation domain (AD), a nuclear localization sequence (NLS) and a DNA binding domain (DBD) consisting of 13 C2H2-type zinc fingers. Studies in mouse embryonic and adult hematopoietic stem cells showed that this gene was required as a transcriptional regulator for self-renewal of both stem cell types, but it was dispensable for growth and differentiation of their progeny. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2010]

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