MLLT6 (NM_005937) Human Tagged ORF Clone

CAT#: RC214043

MLLT6 (Myc-DDK-tagged)-Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), translocated to, 6 (MLLT6)

Product Images

Circular map for RC214043

• 

  Western blot validation of overexpression lysate (Cat# LY416978) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214043 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MLLT6
• Synonyms: AF17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214043 representing NM_005937
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGAGATGGTAGGAGGCTGCTGCGTATGTTCGGACGAGAGGGGCTGGGCCGAGAACCCGCTGGTCT
  ACTGCGATGGGCACGCGTGCAGCGTGGCCGTCCACCAAGCTTGCTATGGCATCGTTCAGGTGCCAACGGG
  ACCCTGGTTCTGCCGGAAATGTGAATCTCAGGAGCGAGCAGCCAGGGTGAGGTGTGAGCTGTGCCCACAC
  AAAGACGGGGCATTGAAGAGGACTGATAATGGAGGCTGGGCACACGTGGTGTGTGCCCTCTACATCCCCG
  AGGTGCAATTTGCCAACGTGCTCACCATGGAGCCCATCGTGCTGCAGTACGTGCCTCATGATCGCTTCAA
  CAAGACCTGTTACATCTGCGAGGAGCAGGGCCGGGAGAGCAAGGCGGCCTCGGGAGCCTGCATGACCTGT
  AACCGCCATGGATGTCGACAAGCTTTCCACGTCACCTGTGCCCAAATGGCAGGCTTGCTGTGTGAGGAAG
  AAGTGCTGGAGGTGGACAACGTCAAGTACTGCGGCTACTGCAAATACCACTTCAGCAAGATGAAGACATC
  CCGGCACAGCAGCGGGGGAGGCGGAGGAGGCGCTGGAGGAGGAGGTGGCAGCATGGGGGGAGGTGGCAGT
  GGTTTCATCTCTGGGAGGAGAAGCCGGTCAGCCTCACCATCCACGCAGCAGGAGAAGCACCCCACCCACC
  ACGAGAGGGGCCAGAAGAAGAGTCGAAAGGACAAAGAACGCCTTAAGCAGAAGCACAAGAAGCGGCCTGA
  GTCGCCCCCCAGCATCCTCACCCCGCCCGTGGTCCCCACTGCTGACAAGGTCTCCTCCTCGGCTTCCTCT
  TCCTCCCACCACGAGGCCAGCACGCAGGAGACCTCTGAGAGCAGCAGGGAGTCAAAGGGGAAAAAGTCTT
  CCAGCCATAGCCTGAGTCATAAAGGGAAGAAACTGAGCAGTGGGAAAGGTGTGAGCAGTTTTACCTCCGC
  CTCCTCTTCTTCCTCCTCCTCTTCCTCCTCCTCTGGGGGGCCCTTCCAGCCTGCAGTCTCGTCCCTGCAG
  AGCTCCCCTGACTTCTCTGCATTCCCCAAGCTGGAGCAGCCAGAGGAGGACAAGTACTCCAAGCCCACAG
  CCCCCGCCCCTTCAGCCCCTCCTTCTCCCTCAGCTCCCGAGCCCCCCAAGGCTGACCTTTTTGAGCAGAA
  GGTGGTCTTCTCTGGCTTTGGGCCCATCATGCGCTTCTCCACCACCACCTCCAGCTCAGGCCGGGCCCGG
  GCGCCCTCCCCTGGGGACTATAAGTCTCCCCACGTCACGGGGTCTGGGGCCTCGGCAGGCACCCACAAAC
  GGATGCCCGCACTGAGTGCCACCCCTGTGCCTGCTGATGAGACCCCTGAGACAGGCCTGAAGGAGAAGAA
  GCACAAAGCCAGCAAGAGGAGCCGCCATGGGCCAGGCCGTCCCAAGGGCAGCCGGAACAAGGAGGGCACT
  GGGGGCCCAGCTGCCCCATCCTTGCCCAGTGCCCAGCTGGCTGGCTTTACCGCCACTGCTGCCTCACCCT
  TCTCTGGAGGTTCCCTGGTCAGCTCCGGCCTGGGAGGTCTGTCCTCCCGAACCTTTGGGCCTTCTGGGAG
  CTTGCCCAGCTTGAGCCTGGAGTCCCCCTTACTAGGGGCAGGCATCTACACCAGTAATAAGGACCCCATC
  TCCCACAGTGGCGGGATGCTGCGGGCTGTCTGCAGCACCCCTCTCTCCTCCAGCCTCCTGGGGCCCCCAG
  GGACCTCGGCCCTGCCCCGCCTCAGCCGCTCCCCGTTCACCAGCACCCTCCCCTCCTCTTCTGCTTCTAT
  CTCCACCACTCAGGTGTTTTCTCTGGCTGGCTCTACCTTTAGCCTCCCTTCTACCCACATCTTTGGAACC
  CCCATGGGTGCCGTTAATCCCCTCCTCTCCCAAGCTGAGAGCAGCCACACAGAGCCAGACCTGGAGGACT
  GCAGCTTCCGGTGTCGGGGGACCTCCCCTCAGGAGAGTCTGTCTTCCATGTCCCCCATCAGCAGCCTCCC
  CGCACTCTTCGACCAGACAGCCTCTGCACCCTGTGGGGGCGGCCAGTTAGACCCGGCGGCCCCAGGGACG
  ACTAACATGGAGCAGCTTCTGGAGAAGCAGGGCGACGGGGAGGCCGGCGTCAACATCGTGGAGATGCTGA
  AGGCGCTGCACGCGCTGCAGAAGGAGAACCAGCGGCTGCAAGAGCAGATCCTGAGCCTGACGGCCAAAAA
  GGAGCGGCTGCAGATTCTCAACGTGCAGCTCTCTGTGCCCTTCCCTGCCCTGCCTGCTGCCCTGCCTGCC
  GCCAACGGCCCTGTCCCTGGGCCCTATGGCCTGCCTCCCCAAGCCGGCAGCAGCGACTCCTTGAGCACCA
  GCAAGAGCCCTCCGGGAAAGAGCAGCCTCGGCCTGGACAACTCGCTGTCCACTTCTTCTGAGGACCCACA
  CTCAGGCTGCCCGAGCCGCAGCAGCTCGTCGCTGTCCTTCCACAGCACGCCCCCACCGCTGCCCCTCCTC
  CAGCAGAGCCCTGCCACTCTGCCCCTGGCCCTGCCTGGGGCCCCTGCCCCACTCCCGCCCCAGCCGCAGA
  ACGGGTTGGGCCGGGCACCCGGGGCAGCGGGGCTGGGGGCCATGCCCATGGCTGAGGGGCTGTTGGGGGG
  GCTGGCAGGCAGTGGGGGCCTGCCCCTCAATGGGCTCCTTGGGGGGTTGAATGGGGCCGCTGCCCCCAAC
  CCCGCAAGCTTGAGCCAGGCTGGCGGGGCCCCCACGCTGCAGCTGCCAGGCTGTCTCAACAGCCTTACAG
  AGCAGCAGAGACATCTCCTTCAGCAGCAAGAGCAGCAGCTCCAGCAACTCCAGCAGCTCCTGGCCTCCCC
  GCAGCTGACCCCGGAACACCAGACTGTTGTCTACCAGATGATCCAGCAGATCCAGCAGAAACGGGAGCTG
  CAGCGCCTGCAGATGGCTGGGGGCTCCCAGCTGCCCATGGCCAGCCTGCTGGCAGGAAGCTCCACCCCGC
  TGCTGTCTGCGGGTACCCCTGGCCTGCTGCCCACAGCGTCTGCTCCACCCCTGCTGCCCGCTGGAGCCCT
  AGTGGCTCCCTCGCTTGGCAACAACACAAGTCTCATGGCCGCAGCAGCTGCAGCTGCAGCAGTAGCAGCA
  GCAGGCGGACCTCCAGTCCTCACTGCCCAGACCAACCCCTTCCTCAGCCTGTCGGGAGCAGAGGGCAGTG
  GCGGTGGCCCCAAAGGAGGGACCGCTGACAAAGGAGCCTCAGCCAACCAGGAAAAAGGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214043 representing NM_005937
  Red=Cloning site Green=Tags(s)
  
  MKEMVGGCCVCSDERGWAENPLVYCDGHACSVAVHQACYGIVQVPTGPWFCRKCESQERAARVRCELCPH
  KDGALKRTDNGGWAHVVCALYIPEVQFANVLTMEPIVLQYVPHDRFNKTCYICEEQGRESKAASGACMTC
  NRHGCRQAFHVTCAQMAGLLCEEEVLEVDNVKYCGYCKYHFSKMKTSRHSSGGGGGGAGGGGGSMGGGGS
  GFISGRRSRSASPSTQQEKHPTHHERGQKKSRKDKERLKQKHKKRPESPPSILTPPVVPTADKVSSSASS
  SSHHEASTQETSESSRESKGKKSSSHSLSHKGKKLSSGKGVSSFTSASSSSSSSSSSSGGPFQPAVSSLQ
  SSPDFSAFPKLEQPEEDKYSKPTAPAPSAPPSPSAPEPPKADLFEQKVVFSGFGPIMRFSTTTSSSGRAR
  APSPGDYKSPHVTGSGASAGTHKRMPALSATPVPADETPETGLKEKKHKASKRSRHGPGRPKGSRNKEGT
  GGPAAPSLPSAQLAGFTATAASPFSGGSLVSSGLGGLSSRTFGPSGSLPSLSLESPLLGAGIYTSNKDPI
  SHSGGMLRAVCSTPLSSSLLGPPGTSALPRLSRSPFTSTLPSSSASISTTQVFSLAGSTFSLPSTHIFGT
  PMGAVNPLLSQAESSHTEPDLEDCSFRCRGTSPQESLSSMSPISSLPALFDQTASAPCGGGQLDPAAPGT
  TNMEQLLEKQGDGEAGVNIVEMLKALHALQKENQRLQEQILSLTAKKERLQILNVQLSVPFPALPAALPA
  ANGPVPGPYGLPPQAGSSDSLSTSKSPPGKSSLGLDNSLSTSSEDPHSGCPSRSSSSLSFHSTPPPLPLL
  QQSPATLPLALPGAPAPLPPQPQNGLGRAPGAAGLGAMPMAEGLLGGLAGSGGLPLNGLLGGLNGAAAPN
  PASLSQAGGAPTLQLPGCLNSLTEQQRHLLQQQEQQLQQLQQLLASPQLTPEHQTVVYQMIQQIQQKREL
  QRLQMAGGSQLPMASLLAGSSTPLLSAGTPGLLPTASAPPLLPAGALVAPSLGNNTSLMAAAAAAAAVAA
  AGGPPVLTAQTNPFLSLSGAEGSGGGPKGGTADKGASANQEKG
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005937
• ORF Size: 3279 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005937.4
• RefSeq Size: 4914 bp
• RefSeq ORF: 3282 bp
• Locus ID: 4302
• UniProt ID: P55198
• Cytogenetics: 17q12
• Protein Families: Druggable Genome
• MW: 111.9 kDa