PGAP1 (NM_024989) Human Tagged ORF Clone

CAT#: RC213837

PGAP1 (Myc-DDK-tagged)-Human post-GPI attachment to proteins 1 (PGAP1)

Product Images

Circular map for RC213837

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PGAP1
• Synonyms: Bst1; ISPD3024; MRT42; NEDDSBA; SPG67
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213837 representing NM_024989
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTCTTCACTCAGTTAATCTCTGGAACCTGGCGTTTTATGTCTTCATGGTCTTTCTGGCAACCCTGG
  GGCTGTGGGATGTCTTCTTCGGCTTCGAGGAGAATAAGTGCAGTATGAGCTACATGTTTGAGTACCCGGA
  GTATCAGAAAATAGAACTTCCAAAGAAACTGGCAAAACGCTATCCCGCATATGAGTTGTATCTTTATGGA
  GAGGGATCCTATGCTGAAGAACACAAAATTCTCCCTTTGACGGGTATTCCAGTTCTCTTTCTTCCTGGTA
  ATGCTGGAAGTTATAAGCAAGTTCGTTCTATTGGCTCCATTGCACTTAGAAAAGCAGAGGACATTGACTT
  CAAGTACCACTTTGACTTCTTTAGTGTGAACTTCAATGAAGAACTGGTGGCTTTGTATGGTGGAAGTCTT
  CAGAAGCAGACCAAGTTTGTACATGAATGTATTAAAACAATTCTCAAACTCTATAAGGGTCAAGAATTTG
  CTCCAAAAAGTGTGGCAATAATTGGTCATTCTATGGGTGGCCTTGTTGCAAGAGCATTGCTTACACTGAA
  AAATTTTAAGCATGATCTGATAAATCTTCTTATTACACAAGCCACACCTCATGTTGCTCCTGTGATGCCA
  TTAGATCGTTTCATTACAGATTTTTATACGACTGTAAACAACTATTGGATTCTAAATGCTCGACACATAA
  ATTTAACCACACTTTCTGTAGCTGGAGGATTCCGGGATTACCAAGTTCGTTCAGGATTGACTTTTCTACC
  AAAATTAAGCCATCATACCAGTGCCTTATCTGTTGTGAGTTCAGCAGTGCCTAAGACCTGGGTCTCAACA
  GACCACCTCTCCATTGTGTGGTGTAAACAATTGCAGTTGACTACAGTTCGAGCATTCTTTGATCTTATTG
  ATGCTGATACTAAACAAATAACTCAAAATTCCAAGAAGAAACTGTCAGTTTTGTATCACCACTTTATAAG
  ACACCCATCAAAACATTTTGAGGAAAATCCAGCTATAATTTCTGACTTAACAGGGACATCTATGTGGGTT
  CTAGTAAAAGTGTCCAAATGGACCTATGTAGCTTACAACGAATCTGAGAAGATATATTTTACATTTCCTC
  TTGAAAATCATAGAAAAATCTACACTCATGTCTATTGTCAGAGCACTATGCTGGATACAAATAGTTGGAT
  TTTTGCTTGCATAAACAGCACTTCTATGTGCCTGCAAGGGGTTGATTTATCATGGAAAGCTGAACTGCTG
  CCAACAATTAAGTATCTGACATTAAGACTTCAAGACTATCCATCTTTGTCTCATCTTGTTGTTTATGTAC
  CATCTGTTCGTGGAAGTAAGTTTGTTGTAGATTGTGAATTCTTTAAAAAAGAGAAAAGATACATACAGCT
  TCCTGTAACTCATCTTTTTTCCTTTGGATTGTCTTCAAGGAAAGTGGTGTTAAATACAAATGGCCTATAC
  TACAATCTAGAGCTTCTGAACTTTGGACAGATATACCAAGCTTTTAAAATCAACGTGGTAAGCAAGTGCT
  CAGCAGTCAAAGAAGAAATAACCAGTATCTATAGACTTCATATTCCTTGGTCTTATGAAGATTCACTAAC
  CATTGCACAGGCTCCATCTTCCACAGAAATTTCTCTGAAACTCCATATTGCTCAACCAGAAAACAATACC
  CATGTGGCATTATTTAAAATGTACACGTCATCAGACTGTCGGTACGAGGTGACAGTTAAGACTTCCTTTT
  CACAAATACTTGGACAGGTAGTTAGATTTCATGGTGGAGCTCTTCCTGCTTATGTCGTATCTAATATCCT
  TCTTGCTTATAGAGGACAGTTATATTCTCTTTTCTCAACAGGTTGTTGCTTAGAATATGCTACCATGTTG
  GATAAAGAAGCCAAACCATACAAAGTTGATCCTTTTGTAATTATCATTAAGTTTCTGTTGGGGTATAAAT
  GGTTTAAAGAATTGTGGGATGTATTATTGTTACCAGAATTAGATGCCGTCATTTTAACTTGTCAGAGTAT
  GTGTTTCCCCCTGATATCCTTGATTCTCTTTCTGTTTGGAACGTGTACTGCCTACTGGAGTGGCCTACTG
  TCTTCTGCATCTGTGAGACTTCTTTCTTCATTGTGGCTAGCTTTGAAAAGACCCTCTGAACTTCCTAAAG
  ATATCAAGATGATATCACCAGACTTGCCCTTTTTGACAATTGTCTTGATCATAGTTAGTTGGACAACTTG
  TGGAGCACTAGCCATACTTCTTTCTTATCTTTACTATGTGTTTAAGGTTGTTCATCTGCAAGCCAGCTTA
  ACAACTTTTAAGAATAGCCAGCCTGTGAATCCCAAACACTCTAGAAGAAGTGAAAAGAAATCCAATCATC
  ATAAAGACTCCTCAATACACCATCTTCGTTTATCTGCCAACGATGCTGAAGATAGCCTTCGCATGCACAG
  TACTGTGATTAACTTACTAACATGGATTGTATTACTCAGCATGCCTTCTCTAATTTATTGGCTAAAGAAT
  CTTAGGTATTATTTTAAACTTAATCCTGATCCATGTAAACCTTTGGCATTTATCCTTATTCCGACTATGG
  CAATTCTTGGAAATACTTACACTGTTTCAATAAAATCAAGTAAATTGTTGAAGACTACTTCACAATTTCC
  ACTTCCTCTGGCTGTTGGTGTGATTGCTTTTGGGTCAGCACATTTATATAGGCTTCCATGCTTTGTCTTC
  ATTCCTCTTTTACTCCATGCATTATGCAACTTTATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213837 representing NM_024989
  Red=Cloning site Green=Tags(s)
  
  MFLHSVNLWNLAFYVFMVFLATLGLWDVFFGFEENKCSMSYMFEYPEYQKIELPKKLAKRYPAYELYLYG
  EGSYAEEHKILPLTGIPVLFLPGNAGSYKQVRSIGSIALRKAEDIDFKYHFDFFSVNFNEELVALYGGSL
  QKQTKFVHECIKTILKLYKGQEFAPKSVAIIGHSMGGLVARALLTLKNFKHDLINLLITQATPHVAPVMP
  LDRFITDFYTTVNNYWILNARHINLTTLSVAGGFRDYQVRSGLTFLPKLSHHTSALSVVSSAVPKTWVST
  DHLSIVWCKQLQLTTVRAFFDLIDADTKQITQNSKKKLSVLYHHFIRHPSKHFEENPAIISDLTGTSMWV
  LVKVSKWTYVAYNESEKIYFTFPLENHRKIYTHVYCQSTMLDTNSWIFACINSTSMCLQGVDLSWKAELL
  PTIKYLTLRLQDYPSLSHLVVYVPSVRGSKFVVDCEFFKKEKRYIQLPVTHLFSFGLSSRKVVLNTNGLY
  YNLELLNFGQIYQAFKINVVSKCSAVKEEITSIYRLHIPWSYEDSLTIAQAPSSTEISLKLHIAQPENNT
  HVALFKMYTSSDCRYEVTVKTSFSQILGQVVRFHGGALPAYVVSNILLAYRGQLYSLFSTGCCLEYATML
  DKEAKPYKVDPFVIIIKFLLGYKWFKELWDVLLLPELDAVILTCQSMCFPLISLILFLFGTCTAYWSGLL
  SSASVRLLSSLWLALKRPSELPKDIKMISPDLPFLTIVLIIVSWTTCGALAILLSYLYYVFKVVHLQASL
  TTFKNSQPVNPKHSRRSEKKSNHHKDSSIHHLRLSANDAEDSLRMHSTVINLLTWIVLLSMPSLIYWLKN
  LRYYFKLNPDPCKPLAFILIPTMAILGNTYTVSIKSSKLLKTTSQFPLPLAVGVIAFGSAHLYRLPCFVF
  IPLLLHALCNFM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024989
• ORF Size: 2766 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024989.4
• RefSeq Size: 11113 bp
• RefSeq ORF: 2769 bp
• Locus ID: 80055
• UniProt ID: Q75T13
• Cytogenetics: 2q33.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
• MW: 105.4 kDa
• Gene Summary: The protein encoded by this gene functions early in the glycosylphosphatidylinositol (GPI) biosynthetic pathway, catalyzing the inositol deacylation of GPI. The encoded protein is required for the production of GPI that can attach to proteins, and this may be an important factor in the transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi. Defects in this gene are a cause an autosomal recessive form of cognitive impairment. [provided by RefSeq, Jul 2017]