Paxillin (PXN) (NM_002859) Human Tagged ORF Clone

CAT#: RC213811

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PXN (Myc-DDK-tagged)-Human paxillin (PXN), transcript variant 2



Reconstitution Protocol

USD 778.00

In Stock*

Size
    • 10 ug

Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PXN
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC213811 representing NM_002859
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGACCTCGACGCCCTGCTGGCGGACTTGGAGTCTACCACCTCCCACATCTCCAAACGGCCTGTGT
TCTTGTCGGAGGAGACCCCCTACTCATACCCAACTGGAAACCACACATACCAGGAGATTGCCGTGCCACC
CCCCGTCCCCCCACCCCCGTCCAGCGAGGCCCTCAATGGCACAATCCTTGACCCCTTAGACCAGTGGCAG
CCCAGCGGCTCCCGATTCATCCACCAGCAGCCTCAGTCCTCATCACCTGTGTACGGCTCCAGTGCCAAAA
CTTCCAGTGTCTCCAACCCTCAGGACAGTGTTGGCTCTCCGTGCTCCCGAGTGGGTGAGGAGGAGCACGT
CTACAGCTTCCCCAACAAGCAGAAATCAGCTGAGCCTTCACCCACCGTAATGAGCACGTCCCTGGGCAGC
AACCTTTCTGAACTCGACCGCCTGCTGCTGGAACTGAACGCTGTACAGCATAACCCGCCAGGCTTCCCTG
CAGATGAGGCCAACTCAAGCCCCCCGCTTCCTGGGGCCCTGAGCCCCCTCTATGGTGTCCCAGAGACTAA
CAGCCCCTTGGGAGGCAAAGCTGGGCCCCTGACGAAAGAGAAGCCTAAGCGGAATGGGGGCCGGGGCCTG
GAGGACGTGCGGCCCAGTGTGGAGAGTCTCTTGGATGAACTGGAGAGCTCCGTGCCCAGCCCCGTCCCTG
CCATCACTGTGAACCAGGGCGAGATGAGCAGCCCGCAGCGCGTCACCTCCACCCAACAGCAGACACGCAT
CTCGGCCTCCTCTGCCACCAGGGAGCTGGACGAGCTGATGGCTTCGCTGTCGGATTTCAAGTTCATGGCC
CAGGGGAAGACAGGGAGCAGCTCACCCCCTGGGGGGCCCCCGAAGCCCGGGAGCCAGCTGGACAGCATGC
TGGGGAGCCTGCAGTCTGACCTGAACAAGCTGGGGGTCGCCACAGTCGCCAAAGGAGTCTGCGGGGCCTG
CAAGAAGCCCATCGCCGGGCAGGTTGTGACCGCCATGGGGAAGACGTGGCACCCCGAGCACTTCGTCTGC
ACCCACTGCCAGGAGGAGATCGGATCCCGGAACTTCTTCGAGCGGGATGGACAGCCCTACTGTGAAAAGG
ACTACCACAACCTCTTCTCCCCGCGCTGCTACTACTGCAACGGCCCCATCCTGGATAAAGTGGTGACAGC
CCTTGACCGGACGTGGCACCCTGAACACTTCTTCTGTGCACAGTGTGGAGCCTTCTTTGGTCCCGAAGGG
TTCCACGAGAAGGACGGCAAGGCCTACTGTCGCAAGGACTACTTCGACATGTTCGCACCCAAGTGTGGCG
GCTGCGCCCGGGCCATCCTGGAGAACTATATCTCAGCCCTCAACACGCTGTGGCATCCTGAGTGCTTTGT
GTGCCGGGAATGCTTCACGCCATTCGTGAACGGCAGCTTCTTCGAGCACGACGGGCAGCCCTACTGTGAG
GTGCACTACCACGAGCGGCGCGGCTCGCTGTGTTCTGGCTGCCAGAAGCCCATCACCGGCCGCTGCATCA
CCGCCATGGCCAAGAAGTTCCACCCCGAGCACTTCGTCTGTGCCTTCTGCCTCAAGCAGCTCAACAAGGG
CACCTTCAAGGAGCAGAACGACAAGCCTTACTGTCAGAACTGCTTCCTCAAGCTCTTCTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC213811 representing NM_002859
Red=Cloning site Green=Tags(s)

MDDLDALLADLESTTSHISKRPVFLSEETPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTILDPLDQWQ
PSGSRFIHQQPQSSSPVYGSSAKTSSVSNPQDSVGSPCSRVGEEEHVYSFPNKQKSAEPSPTVMSTSLGS
NLSELDRLLLELNAVQHNPPGFPADEANSSPPLPGALSPLYGVPETNSPLGGKAGPLTKEKPKRNGGRGL
EDVRPSVESLLDELESSVPSPVPAITVNQGEMSSPQRVTSTQQQTRISASSATRELDELMASLSDFKFMA
QGKTGSSSPPGGPPKPGSQLDSMLGSLQSDLNKLGVATVAKGVCGACKKPIAGQVVTAMGKTWHPEHFVC
THCQEEIGSRNFFERDGQPYCEKDYHNLFSPRCYYCNGPILDKVVTALDRTWHPEHFFCAQCGAFFGPEG
FHEKDGKAYCRKDYFDMFAPKCGGCARAILENYISALNTLWHPECFVCRECFTPFVNGSFFEHDGQPYCE
VHYHERRGSLCSGCQKPITGRCITAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQNCFLKLFC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002859
ORF Size 1671 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002859.1, NP_002850.1
RefSeq Size 3595 bp
RefSeq ORF 1674 bp
Locus ID 5829
UniProt ID P49023
Cytogenetics 12q24.23
Domains LIM, Paxillin
Protein Families Druggable Genome, Stem cell - Pluripotency
Protein Pathways Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, VEGF signaling pathway
MW 60.8 kDa
Gene Summary This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]
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