GPR156 (NM_153002) Human Tagged ORF Clone

CAT#: RC213763

GPR156 (Myc-DDK-tagged)-Human G protein-coupled receptor 156 (GPR156), transcript variant 1

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro


  "NM_153002" in other vectors (4)

Reconstitution Protocol

USD 1,118.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

USD 198.00


Rabbit polyclonal anti-GPR156 antibody
    • 100 ul

USD 380.00

Other products for "GPR156"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol GPR156
Synonyms GABABL; PGR28
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC213763 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCTGAAATAAACTGCTCTGAATTGTGTGACAGTTTTCCTGGCCAGGAGCTGGATCGGAGACCCC
TTCATGATCTCTGCAAGACAACAATTACATCTTCCCACCACAGCAGTAAGACCATCTCTTCATTATCTCC
TGTCCTCTTGGGTATTGTTTGGACTTTTCTCAGCTGTGGACTTCTGCTGATACTTTTCTTTCTTGCCTTT
ACAATTCACTGCAGGAAGAACAGGATTGTGAAGATGTCCAGTCCCAATCTGAACATTGTGACCTTACTGG
GCAGTTGTCTCACTTACAGTAGCGCTTACCTCTTTGGGATTCAGGATGTTTTAGTGGGGAGCTCAATGGA
AACTCTCATTCAGACAAGACTGTCCATGCTGTGCATTGGGACCTCCCTTGTGTTTGGCCCCATTCTGGGA
AAGAGCTGGCGACTCTACAAGGTGTTTACCCAAAGGGTCCCGGACAAGAGAGTGATTATCAAAGACCTGC
AGTTGCTGGGGTTGGTGGCAGCCCTGTTGATGGCTGATGTGATCCTGCTCATGACGTGGGTGCTGACTGA
TCCCATCCAGTGCCTCCAGATTCTCAGTGTCAGTATGACGGTGACAGGGAAAGACGTGTCCTGCACTTCG
ACCAGCACCCACTTCTGTGCTTCCCGGTATTCCGATGTTTGGATTGCTCTCATTTGGGGATGCAAGGGTC
TGCTCCTGCTGTATGGTGCCTACCTGGCTGGCCTGACTGGCCATGTCAGCTCCCCTCCTGTGAATCAGTC
CTTAACCATCATGGTGGGGGTCAACCTCCTTGTACTGGCTGCTGGGCTGCTTTTTGTAGTCACCAGATAC
TTGCATTCCTGGCCCAACCTGGTCTTTGGACTCACATCTGGAGGGATCTTTGTTTGTACAACTACAATCA
ACTGCTTCATCTTCATTCCCCAGCTGAAGCAATGGAAGGCATTTGAAGAGGAAAACCAAACAATCAGACG
CATGGCCAAATATTTCAGCACTCCCAACAAAAGCTTCCATACCCAGTATGGTGAGGAGGAGAACTGCCAC
CCGAGGGGAGAGAAAAGCTCCATGGAGAGGCTCCTCACAGAAAAAAATGCTGTGATTGAAAGCCTGCAGG
AACAAGTAAACAACGCCAAAGAGAAGATTGTGAGGCTGATGTCAGCTGAGTGCACCTATGACCTCCCAGA
GGGGGCTGCCCCACCTGCCTCTTCCCCGAACAAGGACGTCCAGGCGGTAGCCTCGGTCCACACCCTGGCA
GCTGCTCAGGGGCCTTCGGGTCACCTCTCTGACTTTCAGAATGATCCTGGCATGGCTGCCCGGGATTCCC
AGTGCACTTCAGGGCCCTCCTCATATGCACAAAGCCTTGAGGGGCCTGGGAAGGACTCCAGCTTCTCCCC
AGGGAAGGAGGAGAAGATATCTGACTCAAAAGACTTTTCTGATCATTTAGACTCAGGTTGTAGCCAGAAG
CCATGGACTGAGCAAAGCCTGGGTCCAGAAAGAGGAGACCAAGTCCCCATGAACCCCAGCCAGAGTCTCC
TACCAGATAGAGGCGGCTCAGATCCCCAGAGACAGAGGCATCTGGAGAACTCAGAGGAGCCCCCAGAGCG
GCGGTCACGGGTTAGTTCAGTAATCAGGGAGAAACTTCAGGAGGTCTTACAAGATCTGGGCCTGGGCCCT
GAGGCTTCCCTCTCCACCGCCCCCTCTTGTCATCAGCAAACCTGGAAGAACAGTGCTGCCTTCAGCCCCC
AAAAGATGCCCCTCTCCAAGGAGCTGGGCTTTAGCCCTTACATGGTGAGGAGAAGGCGGGCAGCTCAGCG
GGCCCGCTCACACTTTCCTGGCTCTGCACCCTCATCTGTGGGGCATCGGGCAAACAGGACTGTTCCTGGG
GCACACAGCAGGCTACATGTGCAGAATGGGGACAGCCCCAGCCTGGCCCCACAAACTACTGATTCCAGAG
TACGAAGACCTTCTTCCAGGAAGCCTTCACTACCTTCAGATCCTCAAGACAGACCAGGTACCCTGGAGGG
CAGCAAACAAAGCCAGACAGAGCCCGAGGGGGCTAGAGGGAGCAAAGCAGCCTTTCTTCGCCAGCCTTCT
GGTTCTGGCCGGGCCCCAAGTCCTGCTGCCCCATGCCTCTCCAAAGCCTCACCTGACTTGCCTGAACAGT
GGCAGCTGTGGCCCCCAGTGCCCTCAGGCTGTGCCTCCCTGTCTTCTCAACACAGCTATTTTGATACTGA
GTCCAGCAGCTCAGATGAGTTCTTCTGCCGCTGCCACCGGCCCTACTGTGAAATCTGCTTCCAGAGCTCT
TCTGACTCTAGTGACAGTGGCACATCAGACACTGACCCTGAGCCTACTGGGGGGCTGGCTTCCTGGGAAA
AGCTGTGGGCCCGCTCCAAGCCTATTGTGAACTTCAAAGATGACTTGAAACCCACGCTGGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC213763 protein sequence
Red=Cloning site Green=Tags(s)

MEPEINCSELCDSFPGQELDRRPLHDLCKTTITSSHHSSKTISSLSPVLLGIVWTFLSCGLLLILFFLAF
TIHCRKNRIVKMSSPNLNIVTLLGSCLTYSSAYLFGIQDVLVGSSMETLIQTRLSMLCIGTSLVFGPILG
KSWRLYKVFTQRVPDKRVIIKDLQLLGLVAALLMADVILLMTWVLTDPIQCLQILSVSMTVTGKDVSCTS
TSTHFCASRYSDVWIALIWGCKGLLLLYGAYLAGLTGHVSSPPVNQSLTIMVGVNLLVLAAGLLFVVTRY
LHSWPNLVFGLTSGGIFVCTTTINCFIFIPQLKQWKAFEEENQTIRRMAKYFSTPNKSFHTQYGEEENCH
PRGEKSSMERLLTEKNAVIESLQEQVNNAKEKIVRLMSAECTYDLPEGAAPPASSPNKDVQAVASVHTLA
AAQGPSGHLSDFQNDPGMAARDSQCTSGPSSYAQSLEGPGKDSSFSPGKEEKISDSKDFSDHLDSGCSQK
PWTEQSLGPERGDQVPMNPSQSLLPDRGGSDPQRQRHLENSEEPPERRSRVSSVIREKLQEVLQDLGLGP
EASLSTAPSCHQQTWKNSAAFSPQKMPLSKELGFSPYMVRRRRAAQRARSHFPGSAPSSVGHRANRTVPG
AHSRLHVQNGDSPSLAPQTTDSRVRRPSSRKPSLPSDPQDRPGTLEGSKQSQTEPEGARGSKAAFLRQPS
GSGRAPSPAAPCLSKASPDLPEQWQLWPPVPSGCASLSSQHSYFDTESSSSDEFFCRCHRPYCEICFQSS
SDSSDSGTSDTDPEPTGGLASWEKLWARSKPIVNFKDDLKPTLV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_153002
ORF Size 2442 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_153002.1, NP_694547.1
RefSeq Size 4200 bp
RefSeq ORF 2445 bp
Locus ID 165829
UniProt ID Q8NFN8
Cytogenetics 3q13.33
Protein Families Druggable Genome, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
MW 89.1 kDa
Gene Summary G protein-coupled receptors (GPCRs) are a large superfamily of cell surface receptors characterized by 7 helical transmembrane domains, together with N-terminal extracellular and C-terminal intracellular domains.[supplied by OMIM, Mar 2008]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.