PGAM4 (NM_001029891) Human Tagged ORF Clone

USD 98.00

USD 390.00


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In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Product Name PGAM4 (NM_001029891) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PGAM4
Synonyms dJ1000K24.1; PGAM-B; PGAM1; PGAM3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC213480 representing NM_001029891
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCCTACAAACTGGTGCTGATCCGGCACGGCGAGAGCACATGGAACCTGGAGAACCGCTTCAGCT
GCTGGTACGACGCCGATCTGAGCCCGGCGGGCCACGAGGAGGCGAAGCGCGGCGGGCAGGCGCTACGAGA
TGCTGGCTATGAGTTTGACATCTGCCTCACCTCAGTGCAGAAGAGAGTGATCCGGACCCTCTGGACAGTG
CTAGATGCCATTGATCAGATGTGGCTGCCAGTGGTGAGGACTTGGCGCCTCAATGAGCGGCACTATGGGG
GTCTAACCGGTCTCAATAAAGCAGAAACTGCTGCAAAGCATGGTGAGGCCCAGGTGAAGATCTGGAGGCG
CTCCTATGATGTCCCACCACCTCCGATGGAGCCCGACCATCCTTTCTACAGCAACATCAGTAAGGATCGC
AGGTATGCAGACCTCACAGAAGATCAGCTACCCTCCTATGAGAGTCCGAAGGATACTATTGCCAGAGCTC
TGCCCTTCTGGAATGAAGAAATAGTTCCCCAGATCAAGGAGGGGAAACGTGTACTGATTGCAGCCCATGG
CAACAGCCTCCAGGGCATTGCCAAGCATGTGGAGGGTCTCTCTGAAGAGGCTATCATGGAGCTGAACCTG
CCGACTGGTATTCCCATCGTCTATGAATTGGACAAGAACTTGAAGCCTATCAAGCCCATGCAGTTTCTGG
GGGATGAAGAGACGGTGTGCAAAGCCATAGAAGCTGTGGCTGCCCAGGGCAAGGCCAAGAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC213480 representing NM_001029891
Red=Cloning site Green=Tags(s)

MAAYKLVLIRHGESTWNLENRFSCWYDADLSPAGHEEAKRGGQALRDAGYEFDICLTSVQKRVIRTLWTV
LDAIDQMWLPVVRTWRLNERHYGGLTGLNKAETAAKHGEAQVKIWRRSYDVPPPPMEPDHPFYSNISKDR
RYADLTEDQLPSYESPKDTIARALPFWNEEIVPQIKEGKRVLIAAHGNSLQGIAKHVEGLSEEAIMELNL
PTGIPIVYELDKNLKPIKPMQFLGDEETVCKAIEAVAAQGKAKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_001029891
ORF Size 762 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001029891.1, NM_001029891.2, NP_001025062.1
RefSeq Size 1678
RefSeq ORF 765
Locus ID 441531
Cytogenetics Xq21.1
Protein Pathways Glycolysis / Gluconeogenesis, Metabolic pathways
MW 28.6 kDa
Gene Summary This intronless gene appears to have arisen from a retrotransposition event, yet it is thought to be an expressed, protein-coding gene. The encoded protein is a member of the phosphoglycerate mutase family, a set of enzymes that catalyze the transfer of a phosphate group from 3-phosphoglycerate to 2-phosphoglycerate. [provided by RefSeq, May 2010]

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