Zonula occludens protein 3 (TJP3) (NM_014428) Human Tagged ORF Clone

CAT#: RC213399

TJP3 (Myc-DDK-tagged)-Human tight junction protein 3 (zona occludens 3) (TJP3)

Product Images

Circular map for RC213399

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  Western blot validation of overexpression lysate (Cat# LY415277) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213399 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TJP3 protein (Cat# TP313399). The protein was produced from HEK293T cells transfected with TJP3 cDNA clone (Cat# RC213399) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zonula occludens protein 3
• Synonyms: ZO-3; ZO3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213399 representing NM_014428
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCTGTGTGGCCTCATGCCCATCTTCCCCGCTCCCCTCGACCAGGTGGCTGACATGGAGGAGCTGA
  CCATCTGGGAACAGCACACGGCCACACTGTCCAAGGACCCCCGCCGGGGCTTTGGCATTGCGATCTCTGG
  AGGCCGAGACCGGCCCGGTGGATCCATGGTTGTATCTGACGTGGTACCTGGAGGGCCGGCGGAGGGCAGG
  CTACAGACAGGCGACCACATCGTCATGGTGAACGGGGTTTCCATGGAGAATGCCACCTCCGCGTTTGCCA
  TTCAGATACTCAAGACCTGCACCAAGATGGCCAACATCACAGTGAAACGTCCCCGGAGGATCCACCTGCC
  CGCCACCAAAGCCAGCCCCTCCAGCCCAGGGCGCCAGGACTCGGATGAAGACGATGGGCCCCAGCGGGTG
  GAGGAGGTGGACCAGGGCCGGGGCTATGACGGCGACTCATCCAGTGGCTCCGGCCGCTCCTGGGACGAGC
  GCTCCCGCCGGCCGAGGCCTGGTCGCCGGGGCCGGGCCGGCAGCCATGGGCGTAGGAGCCCAGGTGGTGG
  CTCTGAGGCCAACGGGCTGGCCCTGGTGTCCGGCTTTAAGCGGCTGCCACGGCAGGACGTGCAGATGAAG
  CCTGTGAAGTCAGTGCTGGTGAAGAGGAGAGACAGCGAAGAGTTTGGCGTCAAGCTGGGCAGTCAGATCT
  TCATCAAGCACATTACAGATTCGGGCCTGGCTGCCCGGCACCGTGGGCTGCAGGAAGGAGATCTCATTCT
  ACAGATCAACGGGGTGTCTAGCCAGAACCTGTCACTGAACGACACCCGGCGACTGATTGAGAAGTCAGAA
  GGGAAGCTAAGCCTGCTGGTGCTGAGAGATCGTGGGCAGTTCCTGGTGAACATTCCGCCTGCTGTCAGTG
  ACAGCGACAGCTCGCCATTGGAGGAAGGCGTGACCATGGCTGATGAGATGTCCTCTCCCCCTGCAGACAT
  CTCGGACCTCGCCTCGGAGCTATCGCAGGCACCACCATCCCACATCCCACCACCACCCCGGCATGCTCAG
  CGGAGCCCCGAGGCCAGCCAGACCGACTCTCCCGTGGAGAGTCCCCGGCTTCGGCGGGAAAGTTCAGTAG
  ATTCCAGAACCATCTCGGAACCAGATGAGCAACGGTCAGAGTTGCCCAGGGAAAGCAGCTATGACATCTA
  CAGAGTGCCCAGCAGTCAGAGCATGGAGGATCGTGGGTACAGCCCCGACACGCGTGTGGTCCGCTTCCTC
  AAGGGCAAGAGCATCGGGCTGCGGCTGGCAGGGGGCAATGACGTGGGCATCTTCGTGTCCGGGGTGCAGG
  CGGGCAGCCCGGCCGACGGGCAGGGCATCCAGGAGGGAGATCAGATTCTGCAGGTGAATGACGTGCCATT
  CCAGAACCTGACACGGGAGGAGGCAGTGCAGTTCCTGCTGGGGCTGCCACCAGGCGAGGAGATGGAGCTG
  GTGACGCAGAGGAAGCAGGACATTTTCTGGAAAATGGTGCAGTCCCGCGTGGGTGACTCCTTCTACATCC
  GCACTCACTTTGAGCTGGAGCCCAGTCCACCGTCTGGCCTGGGCTTCACCCGTGGCGACGTCTTCCACGT
  GCTGGACACGCTGCACCCCGGCCCCGGGCAGAGCCACGCACGAGGAGGCCACTGGCTGGCGGTGCGCATG
  GGTCGTGACCTGCGGGAGCAAGAGCGGGGCATCATTCCCAACCAGAGCAGGGCGGAGCAGCTGGCCAGCC
  TGGAAGCTGCCCAGAGGGCCGTGGGAGTCGGGCCCGGCTCCTCCGCGGGCTCCAATGCTCGGGCCGAGTT
  CTGGCGGCTGCGGGGTCTTCGTCGAGGAGCCAAGAAGACCACTCAGCGGAGCCGTGAGGACCTCTCAGCT
  CTGACCCGACAGGGCCGCTACCCGCCCTACGAACGAGTGGTGTTGCGAGAAGCCAGTTTCAAGCGCCCGG
  TAGTGATCCTGGGACCCGTGGCCGACATTGCTATGCAGAAGTTGACTGCTGAGATGCCTGACCAGTTTGA
  AATCGCAGAGACTGTGTCCAGGACCGACAGCCCCTCCAAGATCATCAAACTAGACACCGTGCGGGTGATT
  GCAGAAAAAGACAAGCATGCGCTCCTGGATGTGACCCCCTCCGCCATCGAGCGCCTCAACTATGTGCAGT
  ACTACCCCATTGTGGTCTTCTTCATCCCCGAGAGCCGGCCGGCCCTCAAGGCACTGCGCCAGTGGCTGGC
  GCCTGCCTCCCGCCGCAGCACCCGTCGCCTCTACGCACAAGCCCAGAAGCTGCGAAAACACAGCAGCCAC
  CTCTTCACAGCCACCATCCCTCTGAATGGCACGAGTGACACCTGGTACCAGGAGCTCAAGGCCATCATTC
  GAGAGCAGCAGACGCGGCCCATCTGGACGGCGGAAGATCAGCTGGATGGCTCCTTGGAGGACAACCTAGA
  CCTCCCTCACCACGGCCTGGCCGACAGCTCCGCTGACCTCAGCTGCGACAGCCGCGTTAACAGCGACTAC
  GAGACGGACGGCGAGGGCGGCGCGTACACGGATGGCGAGGGCTACACAGACGGCGAGGGGGGGCCCTACA
  CGGATGTGGATGATGAGCCCCCGGCTCCAGCCCTGGCCCGGTCCTCGGAGCCCGTGCAGGCAGATGAGTC
  CCAGAGCCCGAGGGATCGTGGGAGAATCTCGGCTCATCAGGGGGCCCAGGTGGACAGCCGCCACCCCCAG
  GGACAGTGGCGACAGGACAGCATGCGAACCTATGAACGGGAAGCCCTGAAGAAAAAGTTTATGCGAGTAC
  ATGATGCGGAGTCCTCCGATGAAGACGGCTATGACTGGGGTCCGGCCACTGACCTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213399 representing NM_014428
  Red=Cloning site Green=Tags(s)
  
  MNLCGLMPIFPAPLDQVADMEELTIWEQHTATLSKDPRRGFGIAISGGRDRPGGSMVVSDVVPGGPAEGR
  LQTGDHIVMVNGVSMENATSAFAIQILKTCTKMANITVKRPRRIHLPATKASPSSPGRQDSDEDDGPQRV
  EEVDQGRGYDGDSSSGSGRSWDERSRRPRPGRRGRAGSHGRRSPGGGSEANGLALVSGFKRLPRQDVQMK
  PVKSVLVKRRDSEEFGVKLGSQIFIKHITDSGLAARHRGLQEGDLILQINGVSSQNLSLNDTRRLIEKSE
  GKLSLLVLRDRGQFLVNIPPAVSDSDSSPLEEGVTMADEMSSPPADISDLASELSQAPPSHIPPPPRHAQ
  RSPEASQTDSPVESPRLRRESSVDSRTISEPDEQRSELPRESSYDIYRVPSSQSMEDRGYSPDTRVVRFL
  KGKSIGLRLAGGNDVGIFVSGVQAGSPADGQGIQEGDQILQVNDVPFQNLTREEAVQFLLGLPPGEEMEL
  VTQRKQDIFWKMVQSRVGDSFYIRTHFELEPSPPSGLGFTRGDVFHVLDTLHPGPGQSHARGGHWLAVRM
  GRDLREQERGIIPNQSRAEQLASLEAAQRAVGVGPGSSAGSNARAEFWRLRGLRRGAKKTTQRSREDLSA
  LTRQGRYPPYERVVLREASFKRPVVILGPVADIAMQKLTAEMPDQFEIAETVSRTDSPSKIIKLDTVRVI
  AEKDKHALLDVTPSAIERLNYVQYYPIVVFFIPESRPALKALRQWLAPASRRSTRRLYAQAQKLRKHSSH
  LFTATIPLNGTSDTWYQELKAIIREQQTRPIWTAEDQLDGSLEDNLDLPHHGLADSSADLSCDSRVNSDY
  ETDGEGGAYTDGEGYTDGEGGPYTDVDDEPPAPALARSSEPVQADESQSPRDRGRISAHQGAQVDSRHPQ
  GQWRQDSMRTYEREALKKKFMRVHDAESSDEDGYDWGPATDL
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014428
• ORF Size: 2856 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014428.1, NP_055243.1
• RefSeq Size: 2859 bp
• RefSeq ORF: 2858 bp
• Locus ID: 27134
• Cytogenetics: 19p13.3
• Protein Pathways: Tight junction
• MW: 104.6 kDa
• Gene Summary: The protein encoded by this gene is a member of the membrane-associated guanylate kinase-like (MAGUK) protein family which is characterized by members having multiple PDZ domains, a single SH3 domain, and a single guanylate kinase-like (GUK)-domain. In addition, members of the zonula occludens protein subfamily have an acidic domain, a basic arginine-rich region, and a proline-rich domain. The protein encoded by this gene plays a role in the linkage between the actin cytoskeleton and tight-junctions and also sequesters cyclin D1 at tight junctions during mitosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. This gene has a partial pseudogene on chromosome 1. [provided by RefSeq, May 2012]