CD168 (HMMR) (NM_012484) Human Tagged ORF Clone

CAT#: RC213321

HMMR (Myc-DDK-tagged)-Human hyaluronan-mediated motility receptor (RHAMM) (HMMR), transcript variant 2

Product Images

Circular map for RC213321

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HMMR (Cat# RC213321, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HMMR antibody (Cat# TA590241).

• 

  Coomassie blue staining of purified HMMR protein (Cat# TP313321). The protein was produced from HEK293T cells transfected with HMMR cDNA clone (Cat# RC213321) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CD168
• Synonyms: CD168; IHABP; RHAMM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213321 representing NM_012484
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTTTCCTAAGGCGCCCTTGAAACGATTCAATGACCCTTCTGGTTGTGCACCATCTCCAGGTGCTT
  ATGATGTTAAAACTTTAGAAGTATTGAAAGGACCAGTATCCTTTCAGAAATCACAAAGATTTAAACAACA
  AAAAGAATCTAAACAAAATCTTAATGTTGACAAAGATACTACCTTGCCTGCTTCAGCTAGAAAAGTTAAG
  TCTTCGGAATCAAAGGAATCTCAAAAGAATGATAAAGATTTGAAGATATTAGAGAAAGAGATTCGTGTTC
  TTCTACAGGAACGTGGTGCCCAGGACAGGCGGATCCAGGATCTGGAAACTGAGTTGGAAAAGATGGAAGC
  AAGGCTAAATGCTGCACTAAGGGAAAAAACATCTCTCTCTGCAAATAATGCTACACTGGAAAAACAACTT
  ATTGAATTGACCAGGACTAATGAACTACTAAAATCTAAGTTTTCTGAAAATGGTAACCAGAAGAATTTGA
  GAATTCTAAGCTTGGAGTTGATGAAACTTAGAAACAAAAGAGAAACAAAGATGAGGGGTATGATGGCTAA
  GCAAGAAGGCATGGAGATGAAGCTGCAGGTCACCCAAAGGAGTCTCGAAGAGTCTCAAGGGAAAATAGCC
  CAACTGGAGGGAAAACTTGTTTCAATAGAGAAAGAAAAGATTGATGAAAAATCTGAAACAGAAAAACTCT
  TGGAATACATCGAAGAAATTAGTTGTGCTTCAGATCAAGTGGAAAAATACAAGCTAGATATTGCCCAGTT
  AGAAGAAAATTTGAAAGAGAAGAATGATGAAATTTTAAGCCTTAAGCAGTCTCTTGAGGAGAATATTGTT
  ATATTATCTAAACAAGTAGAAGATCTAAATGTGAAATGTCAGCTGCTTGAAAAAGAAAAAGAAGACCATG
  TCAACAGGAATAGAGAACACAACGAAAATCTAAATGCAGAGATGCAAAACTTAAAACAGAAGTTTATTCT
  TGAACAACAGGAACGTGAAAAGCTTCAACAAAAAGAATTACAAATTGATTCACTTCTGCAACAAGAGAAA
  GAATTATCTTCGAGTCTTCATCAGAAGCTCTGTTCTTTTCAAGAGGAAATGGTTAAAGAGAAGAATCTGT
  TTGAGGAAGAATTAAAGCAAACACTGGATGAGCTTGATAAATTACAGCAAAAGGAGGAACAAGCTGAAAG
  GCTGGTCAAGCAATTGGAAGAGGAAGCAAAATCTAGAGCTGAAGAATTAAAACTCCTAGAAGAAAAGCTG
  AAAGGGAAGGAGGCTGAACTGGAGAAAAGTAGTGCTGCTCATACCCAGGCCACCCTGCTTTTGCAGGAAA
  AGTATGACAGTATGGTGCAAAGCCTTGAAGATGTTACTGCTCAATTTGAAAGCTATAAAGCGTTAACAGC
  CAGTGAGATAGAAGATCTTAAGCTGGAGAACTCATCATTACAGGAAAAAGCGGCCAAGGCTGGGAAAAAT
  GCAGAGGATGTTCAGCATCAGATTTTGGCAACTGAGAGCTCAAATCAAGAATATGTAAGGATGCTTCTAG
  ATCTGCAGACCAAGTCAGCACTAAAGGAAACAGAAATTAAAGAAATCACAGTTTCTTTTCTTCAAAAAAT
  AACTGATTTGCAGAACCAACTCAAGCAACAGGAGGAAGACTTTAGAAAACAGCTGGAAGATGAAGAAGGA
  AGAAAAGCTGAAAAAGAAAATACAACAGCAGAATTAACTGAAGAAATTAACAAGTGGCGTCTCCTCTATG
  AAGAACTATATAATAAAACAAAACCTTTTCAGCTACAACTAGATGCTTTTGAAGTAGAAAAACAGGCATT
  GTTGAATGAACATGGTGCAGCTCAGGAACAGCTAAATAAAATAAGAGATTCATATGCTAAATTATTGGGT
  CATCAGAATTTGAAACAAAAAATCAAGCATGTTGTGAAGTTGAAAGATGAAAATAGCCAACTCAAATCGG
  AAGTATCAAAACTCCGCTGTCAGCTTGCTAAAAAAAAACAAAGTGAGACAAAACTTCAAGAGGAATTGAA
  TAAAGTTCTAGGTATCAAACACTTTGATCCTTCAAAGGCTTTTCATCATGAAAGTAAAGAAAATTTTGCC
  CTGAAGACCCCATTAAAAGAAGGCAATACAAACTGTTACCGAGCTCCTATGGAGTGTCAAGAATCATGGA
  AG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213321 representing NM_012484
  Red=Cloning site Green=Tags(s)
  
  MSFPKAPLKRFNDPSGCAPSPGAYDVKTLEVLKGPVSFQKSQRFKQQKESKQNLNVDKDTTLPASARKVK
  SSESKESQKNDKDLKILEKEIRVLLQERGAQDRRIQDLETELEKMEARLNAALREKTSLSANNATLEKQL
  IELTRTNELLKSKFSENGNQKNLRILSLELMKLRNKRETKMRGMMAKQEGMEMKLQVTQRSLEESQGKIA
  QLEGKLVSIEKEKIDEKSETEKLLEYIEEISCASDQVEKYKLDIAQLEENLKEKNDEILSLKQSLEENIV
  ILSKQVEDLNVKCQLLEKEKEDHVNRNREHNENLNAEMQNLKQKFILEQQEREKLQQKELQIDSLLQQEK
  ELSSSLHQKLCSFQEEMVKEKNLFEEELKQTLDELDKLQQKEEQAERLVKQLEEEAKSRAEELKLLEEKL
  KGKEAELEKSSAAHTQATLLLQEKYDSMVQSLEDVTAQFESYKALTASEIEDLKLENSSLQEKAAKAGKN
  AEDVQHQILATESSNQEYVRMLLDLQTKSALKETEIKEITVSFLQKITDLQNQLKQQEEDFRKQLEDEEG
  RKAEKENTTAELTEEINKWRLLYEELYNKTKPFQLQLDAFEVEKQALLNEHGAAQEQLNKIRDSYAKLLG
  HQNLKQKIKHVVKLKDENSQLKSEVSKLRCQLAKKKQSETKLQEELNKVLGIKHFDPSKAFHHESKENFA
  LKTPLKEGNTNCYRAPMECQESWK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012484
• ORF Size: 2172 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012484.3
• RefSeq Size: 3002 bp
• RefSeq ORF: 2175 bp
• Locus ID: 3161
• UniProt ID: O75330
• Cytogenetics: 5q34
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: ECM-receptor interaction
• MW: 83.9 kDa
• Gene Summary: The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Dec 2008]