IRF2BP2 (NM_182972) Human Tagged ORF Clone

CAT#: RC213250

IRF2BP2 (Myc-DDK-tagged)-Human interferon regulatory factor 2 binding protein 2 (IRF2BP2), transcript variant 1

Product Images

Circular map for RC213250

• 

  Western blot validation of overexpression lysate (Cat# LY405287) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213250 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IRF2BP2
• Synonyms: CVID14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213250 representing NM_182972
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCGGCGGTGGCGGTGGCGGCCGCGTCCCGGCGGCAGTCGTGCTACCTGTGTGACCTGCCCCGCA
  TGCCCTGGGCCATGATCTGGGACTTCACCGAACCCGTCTGCCGCGGCTGCGTCAACTACGAGGGCGCCGA
  CCGCGTCGAGTTCGTCATCGAGACGGCGCGGCAGCTCAAGCGGGCGCACGGCTGCTTCCCGGAGGGTCGC
  TCCCCACCCGGCGCCGCGGCCTCGGCCGCCGCCAAGCCGCCGCCGCTCTCCGCCAAGGACATCCTTTTGC
  AGCAGCAGCAGCAGCTTGGCCACGGCGGCCCCGAGGCGGCCCCGCGCGCGCCGCAGGCCTTGGAGCGCTA
  CCCGTTGGCGGCCGCGGCCGAGAGGCCCCCGCGCCTCGGCTCTGACTTCGGCAGCAGCCGCCCGGCAGCG
  AGCCTGGCCCAGCCGCCGACGCCGCAGCCGCCGCCCGTGAACGGCATCCTGGTGCCCAACGGCTTCTCCA
  AGCTAGAGGAGCCGCCCGAGCTGAATCGCCAGAGCCCGAACCCGCGGCGCGGCCACGCGGTGCCGCCCAC
  CCTGGTGCCGCTCATGAACGGCTCGGCCACGCCGCTGCCCACCGCGCTCGGCCTCGGCGGCCGCGCTGCC
  GCCTCCTTAGCCGCGGTGTCCGGAACCGCGGCCGCCAGCCTGGGCTCCGCGCAGCCCACCGATCTGGGCG
  CCCACAAGCGGCCGGCATCCGTGTCGAGCAGCGCTGCCGTGGAGCACGAGCAGCGTGAGGCGGCAGCCAA
  GGAGAAACAACCGCCGCCGCCTGCGCACCGGGGCCCGGCCGACAGCCTGTCCACCGCGGCCGGGGCCGCC
  GAGCTGAGCGCGGAAGGTGCGGGCAAGAGCCGCGGGTCTGGAGAGCAGGACTGGGTCAACAGGCCCAAGA
  CCGTGCGCGACACGCTGCTGGCGCTGCACCAGCACGGCCACTCGGGGCCCTTCGAGAGCAAGTTTAAGAA
  GGAGCCGGCCCTGACTGCAGGCAGGTTGTTGGGTTTCGAGGCCAACGGGGCCAACGGGTCTAAAGCAGTT
  GCAAGAACAGCAAGGAAAAGGAAGCCCTCTCCAGAACCAGAAGGTGAAGTCGGGCCCCCTAAGATCAACG
  GAGAGGCCCAGCCGTGGCTGTCCACATCCACAGAGGGGCTCAAGATCCCCATGACTCCTACATCCTCTTT
  TGTGTCTCCGCCACCACCCACTGCCTCACCTCATTCCAACCGGACCACACCGCCTGAAGCGGCCCAGAAT
  GGCCAGTCCCCCATGGCAGCCCTGATCTTAGTAGCAGACAATGCAGGGGGCAGTCATGCCTCAAAAGATG
  CCAACCAGGTTCACTCCACTACCAGGAGGAATAGCAACAGTCCGCCCTCTCCGTCCTCTATGAACCAAAG
  AAGGCTGGGCCCCAGAGAGGTGGGGGGCCAGGGAGCAGGCAACACAGGAGGACTGGAGCCAGTGCACCCT
  GCCAGCCTCCCGGACTCCTCTCTGGCAACCAGTGCCCCGCTGTGCTGCACCCTCTGCCACGAGCGGCTGG
  AGGACACCCATTTTGTGCAGTGCCCGTCCGTCCCTTCGCACAAGTTCTGCTTCCCTTGCTCCAGACAAAG
  CATCAAACAGCAGGGAGCTAGTGGAGAGGTCTATTGTCCCAGTGGGGAAAAATGCCCTCTTGTGGGCTCC
  AATGTCCCCTGGGCCTTTATGCAAGGGGAAATTGCAACCATCCTTGCTGGAGATGTGAAAGTGAAAAAAG
  AGAGAGACTCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213250 representing NM_182972
  Red=Cloning site Green=Tags(s)
  
  MAAAVAVAAASRRQSCYLCDLPRMPWAMIWDFTEPVCRGCVNYEGADRVEFVIETARQLKRAHGCFPEGR
  SPPGAAASAAAKPPPLSAKDILLQQQQQLGHGGPEAAPRAPQALERYPLAAAAERPPRLGSDFGSSRPAA
  SLAQPPTPQPPPVNGILVPNGFSKLEEPPELNRQSPNPRRGHAVPPTLVPLMNGSATPLPTALGLGGRAA
  ASLAAVSGTAAASLGSAQPTDLGAHKRPASVSSSAAVEHEQREAAAKEKQPPPPAHRGPADSLSTAAGAA
  ELSAEGAGKSRGSGEQDWVNRPKTVRDTLLALHQHGHSGPFESKFKKEPALTAGRLLGFEANGANGSKAV
  ARTARKRKPSPEPEGEVGPPKINGEAQPWLSTSTEGLKIPMTPTSSFVSPPPPTASPHSNRTTPPEAAQN
  GQSPMAALILVADNAGGSHASKDANQVHSTTRRNSNSPPSPSSMNQRRLGPREVGGQGAGNTGGLEPVHP
  ASLPDSSLATSAPLCCTLCHERLEDTHFVQCPSVPSHKFCFPCSRQSIKQQGASGEVYCPSGEKCPLVGS
  NVPWAFMQGEIATILAGDVKVKKERDS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182972
• ORF Size: 1761 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182972.2, NP_892017.2
• RefSeq Size: 1900 bp
• RefSeq ORF: 1764 bp
• Locus ID: 359948
• UniProt ID: Q7Z5L9
• Cytogenetics: 1q42.3
• Protein Families: Druggable Genome
• MW: 60.8 kDa
• Gene Summary: This gene encodes an interferon regulatory factor-2 (IRF2) binding protein that interacts with the C-terminal transcriptional repression domain of IRF2. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]