GFM2 (NM_170691) Human Tagged ORF Clone

CAT#: RC213244

GFM2 (Myc-DDK-tagged)-Human G elongation factor, mitochondrial 2 (GFM2), nuclear gene encoding mitochondrial protein, transcript variant 2

Product Images

Circular map for RC213244

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GFM2
• Synonyms: EF-G2mt; EFG2; hEFG2; mEF-G 2; MRRF2; MST027; MSTP027; RRF; RRF2; RRF2mt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213244 representing NM_170691
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGACCAACTTGAGGATATTTGCAATGAGTCATCAGACAATACCCAGTGTGTATATTAATAATATAT
  GCTGCTATAAAATAAGAGCAAGTTTAAAAAGATTAAAGCCACATGTGCCGCTTGGAAGAAATTGCAGTTC
  TCTACCAGGCTTAATAGGAAATGATATCAAATCCCTTCATTCCATCATCAATCCTCCCATAGCTAAAATC
  CGTAATATTGGAATTATGGCTCATATTGATGCAGGCAAAACTACCACCACAGAAAGAATATTGTACTATT
  CCGGATATACAAGATCACTGGGAGATGTTGATGATGGAGACACAGTGACAGATTTCATGGCCCAAGAGCG
  AGAAAGAGGCATTACTATTCAATCAGCTGCTGTTACATTTGATTGGAAAGGTTATAGAGTCAATCTAATT
  GATACACCAGGTCATGTGGACTTTACCTTGGAGGTTGAGCGGTGCCTAAGAGTGTTGGATGGTGCAGTGG
  CTGTATTTGATGCCTCTGCTGGTGTAGAGGCCCAGACTCTCACAGTATGGAGGCAAGCTGATAAACACAA
  TATACCTCGAATCTGTTTTTTAAACAAGATGGACAAAACTGGAGCAAGCTTTAAGTATGCAGTTGAAAGC
  ATCAGAGAGAAGTTAAAGGCAAAGCCTTTGCTTTTACAGTTACCAATTGGTGAAGCCAAAACTTTCAAAG
  GAGTGGTGGATGTAGTAATGAAAGAAAAACTTCTTTGGAATTGCAATTCAAATGATGGAAAAGACTTTGA
  GAGAAAGCCCCTCTTGGAAATGAATGATCCTGAATTGCTGAAGGAAACAACTGAAGCAAGGAATGCCTTA
  ATTGAACAAGTTGCAGATTTGGATGATGAATTTGCTGACTTGGTTTTAGAAGAATTTAGTGAGAATTTTG
  ATTTGTTACCAGCTGAAAAGCTACAGACTGCAATACATAGAGTGACACTAGCTCAGACAGCAGTGCCTGT
  GCTTTGTGGAAGTGCCCTGAAAAACAAAGGGATACAGCCCTTGTTAGATGCTGTTACTATGTACTTACCT
  TCACCTGAAGAGCGTAACTATGAATTTCTGGAGAGAATAAGTCGTCTGCTTTTGCCGTTTGCTGACCAAC
  ATGTAGAAATCCCTTCATTGACTGCTGGTAACATTGCTTTGACTGTTGGGCTTAAACATACTGCCACTGG
  AGACACCATTGTCTCATCCAAGTCCAGTGCATTAGCTGCAGCTCGTAGAGCCGAACGGGAGGGAGAAAAG
  AAGCACAGACAAAACAATGAAGCAGAGAGACTTTTATTGGCTGGAGTGGAGATTCCAGAACCTGTTTTCT
  TCTGTACCATAGAACCCCCATCACTGTCTAAGCAGCCAGATTTGGAACATGCGTTGAAATGTCTTCAGCG
  TGAAGATCCCAGTTTGAAAGTGAGGCTAGATCCTGACTCTGGACAAACTGTTCTGTGTGGTATGGGGGAG
  TTACATATAGAGATTATTCATGATCGAATCAAGAGGGAATATGGACTGGAGACCTATCTCGGGCCTCTCC
  AGGTGGCATATCGAGAGACCATCCTAAACTCAGTTCGTGCCACAGATACCTTAGATAGAACTTTAGGAGA
  CAAAAGGCATCTTGTGACTGTAGAAGTGGAAGCAAGGCCAATTGAAACATCATCTGTTATGCCTGTGATT
  GAGTTTGAGTATGCTGAAAGTATCAATGAAGGCCTTTTGAAGGTCTCCCAAGAGGCCATTGAAAATGGAA
  TTCACAGCGCATGTCTCCAAGGACCATTGCTTGGATCCCCAATTCAGGATGTAGCAATTACTTTACATTC
  CCTGACAATTCATCCTGGCACCTCCACAACTATGATTTCTGCCTGTGTCTCAAGATGCGTGCAAAAGGCT
  CTGAAGAAAGCTGATAAGCAAGTTTTGGAGCCTCTGATGAATCTTGAGGTTACAGTAGCTAGAGATTATC
  TCAGCCCTGTCCTGGCAGATCTGGCACAAAGAAGAGGAAACATTCAGGAAATTCAGACTCGCCAGGACAA
  CAAAGTTGTTATTGGATTTGTTCCCTTAGCAGAAATTATGGGTTATTCAACTGTGCTTCGAACGCTAACA
  TCAGGCTCAGCTACTTTTGCCTTAGAACTATCTACTTATCAAGCCATGAATCCTCAAGATCAAAATACAC
  TGCTCAACCGGAGAAGTGGTTTGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213244 representing NM_170691
  Red=Cloning site Green=Tags(s)
  
  MLTNLRIFAMSHQTIPSVYINNICCYKIRASLKRLKPHVPLGRNCSSLPGLIGNDIKSLHSIINPPIAKI
  RNIGIMAHIDAGKTTTTERILYYSGYTRSLGDVDDGDTVTDFMAQERERGITIQSAAVTFDWKGYRVNLI
  DTPGHVDFTLEVERCLRVLDGAVAVFDASAGVEAQTLTVWRQADKHNIPRICFLNKMDKTGASFKYAVES
  IREKLKAKPLLLQLPIGEAKTFKGVVDVVMKEKLLWNCNSNDGKDFERKPLLEMNDPELLKETTEARNAL
  IEQVADLDDEFADLVLEEFSENFDLLPAEKLQTAIHRVTLAQTAVPVLCGSALKNKGIQPLLDAVTMYLP
  SPEERNYEFLERISRLLLPFADQHVEIPSLTAGNIALTVGLKHTATGDTIVSSKSSALAAARRAEREGEK
  KHRQNNEAERLLLAGVEIPEPVFFCTIEPPSLSKQPDLEHALKCLQREDPSLKVRLDPDSGQTVLCGMGE
  LHIEIIHDRIKREYGLETYLGPLQVAYRETILNSVRATDTLDRTLGDKRHLVTVEVEARPIETSSVMPVI
  EFEYAESINEGLLKVSQEAIENGIHSACLQGPLLGSPIQDVAITLHSLTIHPGTSTTMISACVSRCVQKA
  LKKADKQVLEPLMNLEVTVARDYLSPVLADLAQRRGNIQEIQTRQDNKVVIGFVPLAEIMGYSTVLRTLT
  SGSATFALELSTYQAMNPQDQNTLLNRRSGLT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_170691
• ORF Size: 2196 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_170691.3
• RefSeq Size: 2970 bp
• RefSeq ORF: 2199 bp
• Locus ID: 84340
• UniProt ID: Q969S9
• Cytogenetics: 5q13.3
• MW: 81 kDa
• Gene Summary: Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. This gene encodes one of the mitochondrial translation elongation factors, which is a GTPase that plays a role at the termination of mitochondrial translation by mediating the disassembly of ribosomes from messenger RNA . Its role in the regulation of normal mitochondrial function and in disease states attributed to mitochondrial dysfunction is not known. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2013]