RNF123 (NM_022064) Human Tagged ORF Clone

CAT#: RC213221

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RNF123 (Myc-DDK-tagged)-Human ring finger protein 123 (RNF123)



  "NM_022064" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 680.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol RNF123
Synonyms FP1477; KPC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC213221 representing NM_022064
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATCCAAGGGGGCCGGCATGTCTTTCTCCCGCAAGAGCTATAGGCTGACCTCAGATGCTGAGAAAT
CCAGGGTCACAGGCATTGTGCAGGAGAAGCTGCTGAATGACTACCTGAACCGCATCTTTTCCTCTTCTGA
ACATGCACCCCCAGCAGCCACCAGCAGGAAACCCCTGAACTTCCAGAACCTGCCAGAACATTTGGACCAG
TTGCTACAGGTGGACAATGAGGAGGAGGAAAGCCAGGGACAGGTTGAAGGGCGGCTTGGCCCATCCACTG
TGGTCCTGGACCACACAGGCGGCTTTGAGGGGCTTCTCCTGGTGGATGATGACCTGCTGGGGGTGATTGG
ACACAGCAACTTTGGCACCATCCGCTCTACCACATGCGTGTACAAAGGGAAATGGCTCTACGAGGTCCTC
ATCTCCTCCCAGGGGCTCATGCAGATCGGCTGGTGCACCATCAGCTGCCGCTTCAACCAGGAGGAGGGGG
TTGGAGATACACACAACTCCTATGCCTATGATGGCAACCGCGTGCGCAAGTGGAATGTGACCACAACGAA
TTATGGCAAGGCGTGGGCAGCGGGGGACATCGTGAGCTGCCTGATTGACCTGGATGATGGCACTCTGTCC
TTCTGCCTGAACGGTGTATCACTGGGCACTGCCTTTGAGAACCTGTCCAGGGGCCTGGGTATGGCCTACT
TCCCAGCCATCAGCCTCTCTTTCAAGGAGTCCGTGGCCTTCAACTTTGGCAGCCGTCCTCTGCGCTACCC
AGTGGCAGGCTACCGGCCCCTGCAGGACCCACCGAGTGCTGACCTGGTGCGGGCACAGAGGTTGCTGGGC
TGCTTCCGGGCAGTGCTGAGTGTGGAGCTGGACCCTGTGGAGGGGCGGCTGTTGGACAAGGAGAGCTCCA
AGTGGCGGTTGCGGGGCCAGCCCACCGTCCTCCTCACACTGGCCCACATCTTCCATCACTTTGCACCGCT
TCTGCGCAAGGTGTATCTGGTGGAGGCTGTGCTCATGAGCTTCTTGCTGGGCATCGTGGAGAAGGGCACA
CCCACACAGGCACAGTCCGTGGTGCACCAGGTCCTGGACCTCTTGTGGCTCTTCATGGAGGACTACGAGG
TACAAGATTGCCTCAAGCAGTTGATGATGTCTCTGCTTCGGCTGTACCGATTCTCACCCATTGTCCCAGA
CCTGGGCCTACAGATCCATTACCTGCGGCTCACTATCGCCATCCTGAGGCATGAGAAGTCCCGCAAGTTT
CTGCTTAGCAATGTCCTCTTCGACGTGCTCCGCTCCGTCGTCTTCTTTTACATCAAGAGCCCCCTGCGTG
TGGAGGAGGCCGGCCTGCAGGAGCTCATTCCCACCACCTGGTGGCCCCACTGCTCCAGTAGGGAGGGCAA
AGAGAGCACGGAGATGAAGGAGGAGACCGCAGAGGAGCGGCTGCGGCGGCGAGCCTACGAACGGGGCTGT
CAGCGGCTCAGGAAGCGCATCGAAGTGGTGGAAGAACTACAGGTCCAGATCCTGAAGCTGCTGCTGGACA
ATAAAGATGACAATGGGGGTGAAGCTTCTAGGTATATCTTCCTGACCAAGTTTCGCAAGTTTCTGCAGGA
GAACGCCAGTGGCCGGGGGAACATGCCCATGCTCTGCCCCCCTGAGTACATGGTCTGCTTCTTACACCGG
CTGATCTCTGCCCTGCGCTACTATTGGGATGAATACAAGGCTTCCAATCCTCATGCTTCCTTCAGTGAGG
AGGCCTACATCCCGCCCCAGGTCTTCTATAATGGCAAGGTGGACTACTTTGACCTGCAGCGCCTGGGGGG
CCTCCTCTCGCACCTGCGGAAGACCCTCAAAGATGACCTTGCTTCCAAAGCCAACATTGTGATCGACCCA
CTGGAGCTCCAGTCAACCGCCATGGATGACCTAGATGAGGATGAGGAGCCAGCCCCAGCTATGGCCCAGC
GCCCCATGCAGGCCCTGGCTGTTGGGGGGCCACTGCCCCTGCCCCGGCCCGGCTGGCTCAGTTCTCCAAC
TTTGGGCCGAGCCAACCGCTTCCTCAGCACAGCGGCTGTGAGCCTCATGACCCCACGGCGGCCTCTGAGC
ACCTCGGAGAAAGTGAAGGTCCGCACGCTGAGCGTGGAGCAGAGGACCCGTGAGGACATTGAAGGCAGCC
ACTGGAATGAGGGCTTGCTGCTGGGGCGGCCCCCCGAGGAGCCTGAGCAGCCCCTCACCGAGAACTCGCT
GCTGGAAGTCCTGGATGGGGCGGTCATGATGTACAACCTCAGCGTACACCAGCAGCTGGGCAAGATGGTG
GGTGTCTCCGATGATGTCAATGAATACGCTATGGCTCTGAGGGACACAGAGGACAAGCTCCGCCGGTGCC
CCAAGAGGAGGAAGGACATCCTTGCAGAGTTGACCAAGAGCCAGAAGGTTTTCTCAGAAAAGCTGGACCA
CCTGAGCCGCCGTCTTGCCTGGGTCCATGCCACTGTCTACTCCCAGGAGAAGATGCTGGACATCTACTGG
CTGCTGCGCGTCTGCCTGCGGACCATTGAGCACGGTGATCGCACAGGGTCTCTCTTTGCCTTCATGCCCG
AGTTCTACCTGAGCGTGGCCATCAACAGCTACAGTGCTCTCAAGAATTACTTTGGTCCCGTGCACAGCAT
GGAGGAGCTCCCAGGCTATGAAGAGACCCTGACCCGCCTGGCTGCCATTCTCGCCAAACACTTTGCCGAC
GCACGCATTGTGGGCACTGACATCCGAGACTCACTGATGCAGGCCCTGGCCAGCTACGTGTGCTACCCAC
ACTCCCTGCGGGCTGTGGAGCGAATCCCCGAGGAGCAGCGTATCGCCATGGTGAGGAACCTCCTGGCGCC
CTATGAGCAGCGGCCCTGGGCCCAGACCAACTGGATCCTGGTGCGGCTCTGGAGGGGCTGTGGCTTCGGG
TACCGCTATACACGGCTGCCACATCTGCTGAAAACCAAACTTGAGGACGCCAATTTGCCCAGCCTCCAGA
AGCCCTGCCCTTCCACCCTGCTGCAGCAGCACATGGCGGACCTCCTACAGCAGGGTCCTGATGTGGCACC
CAGCTTCCTCAACAGCGTCCTCAATCAGCTCAACTGGGCCTTCTCTGAATTCATTGGCATGATCCAAGAG
ATCCAGCAGGCTGCTGAGCGCCTGGAGCGGAACTTTGTGGACAGCCGGCAGCTCAAGGTATGTGCCACCT
GCTTTGACCTCTCGGTCAGCCTGCTGCGTGTCTTGGAGATGACTATCACACTGGTGCCTGAGATATTCCT
TGACTGGACCCGGCCTACCTCTGAGATGCTGCTGCGGCGTCTTGCACAGCTGCTAAACCAGGTGCTGAAC
CGGGTGACAGCTGAGAGGAACCTGTTTGATCGTGTGGTCACCCTACGGCTGCCTGGCCTAGAGAGCGTGG
ACCACTATCCCATTCTGGTGGCAGTGACGGGCATCCTGGTGCAGCTCCTGGTGCGTGGCCCAGCCTCAGA
GAGAGAGCAAGCCACATCAGTGCTCCTGGCAGATCCCTGCTTCCAGCTACGCTCAATATGCTATCTCCTG
GGACAGCCAGAGCCCCCAGCACCTGGCACTGCTCTGCCAGCCCCTGACCGGAAGCGCTTCTCCCTGCAGA
GCTATGCGGATTATATCAGTGCCGATGAGCTGGCCCAAGTGGAACAGATGCTGGCGCACCTGACCTCTGC
ATCTGCCCAGGCAGCAGCTGCCTCCCTGCCCACCAGTGAGGAGGACCTCTGCCCCATCTGCTATGCCCAC
CCCATCTCTGCTGTGTTCCAGCCCTGTGGCCACAAGTCCTGCAAAGCCTGTATCAACCAGCACCTGATGA
ACAACAAGGACTGCTTCTTCTGCAAAACCACCATCGTGTCTGTAGAGGACTGGGAGAAGGGAGCCAATAC
GAGTACTACCTCCTCAGCTGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC213221 representing NM_022064
Red=Cloning site Green=Tags(s)

MASKGAGMSFSRKSYRLTSDAEKSRVTGIVQEKLLNDYLNRIFSSSEHAPPAATSRKPLNFQNLPEHLDQ
LLQVDNEEEESQGQVEGRLGPSTVVLDHTGGFEGLLLVDDDLLGVIGHSNFGTIRSTTCVYKGKWLYEVL
ISSQGLMQIGWCTISCRFNQEEGVGDTHNSYAYDGNRVRKWNVTTTNYGKAWAAGDIVSCLIDLDDGTLS
FCLNGVSLGTAFENLSRGLGMAYFPAISLSFKESVAFNFGSRPLRYPVAGYRPLQDPPSADLVRAQRLLG
CFRAVLSVELDPVEGRLLDKESSKWRLRGQPTVLLTLAHIFHHFAPLLRKVYLVEAVLMSFLLGIVEKGT
PTQAQSVVHQVLDLLWLFMEDYEVQDCLKQLMMSLLRLYRFSPIVPDLGLQIHYLRLTIAILRHEKSRKF
LLSNVLFDVLRSVVFFYIKSPLRVEEAGLQELIPTTWWPHCSSREGKESTEMKEETAEERLRRRAYERGC
QRLRKRIEVVEELQVQILKLLLDNKDDNGGEASRYIFLTKFRKFLQENASGRGNMPMLCPPEYMVCFLHR
LISALRYYWDEYKASNPHASFSEEAYIPPQVFYNGKVDYFDLQRLGGLLSHLRKTLKDDLASKANIVIDP
LELQSTAMDDLDEDEEPAPAMAQRPMQALAVGGPLPLPRPGWLSSPTLGRANRFLSTAAVSLMTPRRPLS
TSEKVKVRTLSVEQRTREDIEGSHWNEGLLLGRPPEEPEQPLTENSLLEVLDGAVMMYNLSVHQQLGKMV
GVSDDVNEYAMALRDTEDKLRRCPKRRKDILAELTKSQKVFSEKLDHLSRRLAWVHATVYSQEKMLDIYW
LLRVCLRTIEHGDRTGSLFAFMPEFYLSVAINSYSALKNYFGPVHSMEELPGYEETLTRLAAILAKHFAD
ARIVGTDIRDSLMQALASYVCYPHSLRAVERIPEEQRIAMVRNLLAPYEQRPWAQTNWILVRLWRGCGFG
YRYTRLPHLLKTKLEDANLPSLQKPCPSTLLQQHMADLLQQGPDVAPSFLNSVLNQLNWAFSEFIGMIQE
IQQAAERLERNFVDSRQLKVCATCFDLSVSLLRVLEMTITLVPEIFLDWTRPTSEMLLRRLAQLLNQVLN
RVTAERNLFDRVVTLRLPGLESVDHYPILVAVTGILVQLLVRGPASEREQATSVLLADPCFQLRSICYLL
GQPEPPAPGTALPAPDRKRFSLQSYADYISADELAQVEQMLAHLTSASAQAAAASLPTSEEDLCPICYAH
PISAVFQPCGHKSCKACINQHLMNNKDCFFCKTTIVSVEDWEKGANTSTTSSAA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_022064
ORF Size 3942 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_022064.5
RefSeq Size 4312 bp
RefSeq ORF 3945 bp
Locus ID 63891
UniProt ID Q5XPI4
Cytogenetics 3p21.31
Domains RING
Protein Families Druggable Genome
MW 148.5 kDa
Gene Summary The protein encoded by this gene contains a C-terminal RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions, and an N-terminal SPRY domain. This protein displays E3 ubiquitin ligase activity toward the cyclin-dependent kinase inhibitor 1B which is also known as p27 or KIP1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

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