RNF123 (NM_022064) Human Tagged ORF Clone

CAT#: RC213221

RNF123 (Myc-DDK-tagged)-Human ring finger protein 123 (RNF123)

Product Images

Western blot validation of overexpression lysate (Cat# LY411812) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213221 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RNF123
• Synonyms: FP1477; KPC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213221 representing NM_022064
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCCAAGGGGGCCGGCATGTCTTTCTCCCGCAAGAGCTATAGGCTGACCTCAGATGCTGAGAAAT
  CCAGGGTCACAGGCATTGTGCAGGAGAAGCTGCTGAATGACTACCTGAACCGCATCTTTTCCTCTTCTGA
  ACATGCACCCCCAGCAGCCACCAGCAGGAAACCCCTGAACTTCCAGAACCTGCCAGAACATTTGGACCAG
  TTGCTACAGGTGGACAATGAGGAGGAGGAAAGCCAGGGACAGGTTGAAGGGCGGCTTGGCCCATCCACTG
  TGGTCCTGGACCACACAGGCGGCTTTGAGGGGCTTCTCCTGGTGGATGATGACCTGCTGGGGGTGATTGG
  ACACAGCAACTTTGGCACCATCCGCTCTACCACATGCGTGTACAAAGGGAAATGGCTCTACGAGGTCCTC
  ATCTCCTCCCAGGGGCTCATGCAGATCGGCTGGTGCACCATCAGCTGCCGCTTCAACCAGGAGGAGGGGG
  TTGGAGATACACACAACTCCTATGCCTATGATGGCAACCGCGTGCGCAAGTGGAATGTGACCACAACGAA
  TTATGGCAAGGCGTGGGCAGCGGGGGACATCGTGAGCTGCCTGATTGACCTGGATGATGGCACTCTGTCC
  TTCTGCCTGAACGGTGTATCACTGGGCACTGCCTTTGAGAACCTGTCCAGGGGCCTGGGTATGGCCTACT
  TCCCAGCCATCAGCCTCTCTTTCAAGGAGTCCGTGGCCTTCAACTTTGGCAGCCGTCCTCTGCGCTACCC
  AGTGGCAGGCTACCGGCCCCTGCAGGACCCACCGAGTGCTGACCTGGTGCGGGCACAGAGGTTGCTGGGC
  TGCTTCCGGGCAGTGCTGAGTGTGGAGCTGGACCCTGTGGAGGGGCGGCTGTTGGACAAGGAGAGCTCCA
  AGTGGCGGTTGCGGGGCCAGCCCACCGTCCTCCTCACACTGGCCCACATCTTCCATCACTTTGCACCGCT
  TCTGCGCAAGGTGTATCTGGTGGAGGCTGTGCTCATGAGCTTCTTGCTGGGCATCGTGGAGAAGGGCACA
  CCCACACAGGCACAGTCCGTGGTGCACCAGGTCCTGGACCTCTTGTGGCTCTTCATGGAGGACTACGAGG
  TACAAGATTGCCTCAAGCAGTTGATGATGTCTCTGCTTCGGCTGTACCGATTCTCACCCATTGTCCCAGA
  CCTGGGCCTACAGATCCATTACCTGCGGCTCACTATCGCCATCCTGAGGCATGAGAAGTCCCGCAAGTTT
  CTGCTTAGCAATGTCCTCTTCGACGTGCTCCGCTCCGTCGTCTTCTTTTACATCAAGAGCCCCCTGCGTG
  TGGAGGAGGCCGGCCTGCAGGAGCTCATTCCCACCACCTGGTGGCCCCACTGCTCCAGTAGGGAGGGCAA
  AGAGAGCACGGAGATGAAGGAGGAGACCGCAGAGGAGCGGCTGCGGCGGCGAGCCTACGAACGGGGCTGT
  CAGCGGCTCAGGAAGCGCATCGAAGTGGTGGAAGAACTACAGGTCCAGATCCTGAAGCTGCTGCTGGACA
  ATAAAGATGACAATGGGGGTGAAGCTTCTAGGTATATCTTCCTGACCAAGTTTCGCAAGTTTCTGCAGGA
  GAACGCCAGTGGCCGGGGGAACATGCCCATGCTCTGCCCCCCTGAGTACATGGTCTGCTTCTTACACCGG
  CTGATCTCTGCCCTGCGCTACTATTGGGATGAATACAAGGCTTCCAATCCTCATGCTTCCTTCAGTGAGG
  AGGCCTACATCCCGCCCCAGGTCTTCTATAATGGCAAGGTGGACTACTTTGACCTGCAGCGCCTGGGGGG
  CCTCCTCTCGCACCTGCGGAAGACCCTCAAAGATGACCTTGCTTCCAAAGCCAACATTGTGATCGACCCA
  CTGGAGCTCCAGTCAACCGCCATGGATGACCTAGATGAGGATGAGGAGCCAGCCCCAGCTATGGCCCAGC
  GCCCCATGCAGGCCCTGGCTGTTGGGGGGCCACTGCCCCTGCCCCGGCCCGGCTGGCTCAGTTCTCCAAC
  TTTGGGCCGAGCCAACCGCTTCCTCAGCACAGCGGCTGTGAGCCTCATGACCCCACGGCGGCCTCTGAGC
  ACCTCGGAGAAAGTGAAGGTCCGCACGCTGAGCGTGGAGCAGAGGACCCGTGAGGACATTGAAGGCAGCC
  ACTGGAATGAGGGCTTGCTGCTGGGGCGGCCCCCCGAGGAGCCTGAGCAGCCCCTCACCGAGAACTCGCT
  GCTGGAAGTCCTGGATGGGGCGGTCATGATGTACAACCTCAGCGTACACCAGCAGCTGGGCAAGATGGTG
  GGTGTCTCCGATGATGTCAATGAATACGCTATGGCTCTGAGGGACACAGAGGACAAGCTCCGCCGGTGCC
  CCAAGAGGAGGAAGGACATCCTTGCAGAGTTGACCAAGAGCCAGAAGGTTTTCTCAGAAAAGCTGGACCA
  CCTGAGCCGCCGTCTTGCCTGGGTCCATGCCACTGTCTACTCCCAGGAGAAGATGCTGGACATCTACTGG
  CTGCTGCGCGTCTGCCTGCGGACCATTGAGCACGGTGATCGCACAGGGTCTCTCTTTGCCTTCATGCCCG
  AGTTCTACCTGAGCGTGGCCATCAACAGCTACAGTGCTCTCAAGAATTACTTTGGTCCCGTGCACAGCAT
  GGAGGAGCTCCCAGGCTATGAAGAGACCCTGACCCGCCTGGCTGCCATTCTCGCCAAACACTTTGCCGAC
  GCACGCATTGTGGGCACTGACATCCGAGACTCACTGATGCAGGCCCTGGCCAGCTACGTGTGCTACCCAC
  ACTCCCTGCGGGCTGTGGAGCGAATCCCCGAGGAGCAGCGTATCGCCATGGTGAGGAACCTCCTGGCGCC
  CTATGAGCAGCGGCCCTGGGCCCAGACCAACTGGATCCTGGTGCGGCTCTGGAGGGGCTGTGGCTTCGGG
  TACCGCTATACACGGCTGCCACATCTGCTGAAAACCAAACTTGAGGACGCCAATTTGCCCAGCCTCCAGA
  AGCCCTGCCCTTCCACCCTGCTGCAGCAGCACATGGCGGACCTCCTACAGCAGGGTCCTGATGTGGCACC
  CAGCTTCCTCAACAGCGTCCTCAATCAGCTCAACTGGGCCTTCTCTGAATTCATTGGCATGATCCAAGAG
  ATCCAGCAGGCTGCTGAGCGCCTGGAGCGGAACTTTGTGGACAGCCGGCAGCTCAAGGTATGTGCCACCT
  GCTTTGACCTCTCGGTCAGCCTGCTGCGTGTCTTGGAGATGACTATCACACTGGTGCCTGAGATATTCCT
  TGACTGGACCCGGCCTACCTCTGAGATGCTGCTGCGGCGTCTTGCACAGCTGCTAAACCAGGTGCTGAAC
  CGGGTGACAGCTGAGAGGAACCTGTTTGATCGTGTGGTCACCCTACGGCTGCCTGGCCTAGAGAGCGTGG
  ACCACTATCCCATTCTGGTGGCAGTGACGGGCATCCTGGTGCAGCTCCTGGTGCGTGGCCCAGCCTCAGA
  GAGAGAGCAAGCCACATCAGTGCTCCTGGCAGATCCCTGCTTCCAGCTACGCTCAATATGCTATCTCCTG
  GGACAGCCAGAGCCCCCAGCACCTGGCACTGCTCTGCCAGCCCCTGACCGGAAGCGCTTCTCCCTGCAGA
  GCTATGCGGATTATATCAGTGCCGATGAGCTGGCCCAAGTGGAACAGATGCTGGCGCACCTGACCTCTGC
  ATCTGCCCAGGCAGCAGCTGCCTCCCTGCCCACCAGTGAGGAGGACCTCTGCCCCATCTGCTATGCCCAC
  CCCATCTCTGCTGTGTTCCAGCCCTGTGGCCACAAGTCCTGCAAAGCCTGTATCAACCAGCACCTGATGA
  ACAACAAGGACTGCTTCTTCTGCAAAACCACCATCGTGTCTGTAGAGGACTGGGAGAAGGGAGCCAATAC
  GAGTACTACCTCCTCAGCTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213221 representing NM_022064
  Red=Cloning site Green=Tags(s)
  
  MASKGAGMSFSRKSYRLTSDAEKSRVTGIVQEKLLNDYLNRIFSSSEHAPPAATSRKPLNFQNLPEHLDQ
  LLQVDNEEEESQGQVEGRLGPSTVVLDHTGGFEGLLLVDDDLLGVIGHSNFGTIRSTTCVYKGKWLYEVL
  ISSQGLMQIGWCTISCRFNQEEGVGDTHNSYAYDGNRVRKWNVTTTNYGKAWAAGDIVSCLIDLDDGTLS
  FCLNGVSLGTAFENLSRGLGMAYFPAISLSFKESVAFNFGSRPLRYPVAGYRPLQDPPSADLVRAQRLLG
  CFRAVLSVELDPVEGRLLDKESSKWRLRGQPTVLLTLAHIFHHFAPLLRKVYLVEAVLMSFLLGIVEKGT
  PTQAQSVVHQVLDLLWLFMEDYEVQDCLKQLMMSLLRLYRFSPIVPDLGLQIHYLRLTIAILRHEKSRKF
  LLSNVLFDVLRSVVFFYIKSPLRVEEAGLQELIPTTWWPHCSSREGKESTEMKEETAEERLRRRAYERGC
  QRLRKRIEVVEELQVQILKLLLDNKDDNGGEASRYIFLTKFRKFLQENASGRGNMPMLCPPEYMVCFLHR
  LISALRYYWDEYKASNPHASFSEEAYIPPQVFYNGKVDYFDLQRLGGLLSHLRKTLKDDLASKANIVIDP
  LELQSTAMDDLDEDEEPAPAMAQRPMQALAVGGPLPLPRPGWLSSPTLGRANRFLSTAAVSLMTPRRPLS
  TSEKVKVRTLSVEQRTREDIEGSHWNEGLLLGRPPEEPEQPLTENSLLEVLDGAVMMYNLSVHQQLGKMV
  GVSDDVNEYAMALRDTEDKLRRCPKRRKDILAELTKSQKVFSEKLDHLSRRLAWVHATVYSQEKMLDIYW
  LLRVCLRTIEHGDRTGSLFAFMPEFYLSVAINSYSALKNYFGPVHSMEELPGYEETLTRLAAILAKHFAD
  ARIVGTDIRDSLMQALASYVCYPHSLRAVERIPEEQRIAMVRNLLAPYEQRPWAQTNWILVRLWRGCGFG
  YRYTRLPHLLKTKLEDANLPSLQKPCPSTLLQQHMADLLQQGPDVAPSFLNSVLNQLNWAFSEFIGMIQE
  IQQAAERLERNFVDSRQLKVCATCFDLSVSLLRVLEMTITLVPEIFLDWTRPTSEMLLRRLAQLLNQVLN
  RVTAERNLFDRVVTLRLPGLESVDHYPILVAVTGILVQLLVRGPASEREQATSVLLADPCFQLRSICYLL
  GQPEPPAPGTALPAPDRKRFSLQSYADYISADELAQVEQMLAHLTSASAQAAAASLPTSEEDLCPICYAH
  PISAVFQPCGHKSCKACINQHLMNNKDCFFCKTTIVSVEDWEKGANTSTTSSAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022064
• ORF Size: 3942 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022064.5
• RefSeq Size: 4312 bp
• RefSeq ORF: 3945 bp
• Locus ID: 63891
• UniProt ID: Q5XPI4
• Cytogenetics: 3p21.31
• Domains: RING
• Protein Families: Druggable Genome
• MW: 148.5 kDa
• Gene Summary: The protein encoded by this gene contains a C-terminal RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions, and an N-terminal SPRY domain. This protein displays E3 ubiquitin ligase activity toward the cyclin-dependent kinase inhibitor 1B which is also known as p27 or KIP1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]