UFD1 (NM_001035247) Human Tagged ORF Clone

CAT#: RC213180

UFD1L (Myc-DDK-tagged)-Human ubiquitin fusion degradation 1 like (yeast) (UFD1L), transcript variant 2

Product Images

Circular map for RC213180

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  Western blot validation of overexpression lysate (Cat# LY422127) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213180 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified UFD1 protein (Cat# TP313180). The protein was produced from HEK293T cells transfected with UFD1 cDNA clone (Cat# RC213180) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UFD1
• Synonyms: UFD1L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213180 representing NM_001035247
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTCTTTCAACATGTTCGACCACCCTATTCCCAGGGTCTTCCAAAACCGCTTCTCCACACAGTACC
  GCTGCTTCTCTGTGTCCATGCTAGCAGGGCCTAATGACAGGTCAGATGTGGAGAAAGGAGGGAAGAGCCG
  ACTTAACATTACCTATCCCATGCTGTTCAAACTGACCAATAAGAATTCGGACCGCATGACGCATTGTGGC
  GTGCTGGAGTTTGTGGCTGATGAGGGCATCTGCTACCTCCCACACTGGATGATGCAGAACTTACTCTTGG
  AAGAAGGCGGCCTGGTCCAGGTGGAGAGCGTCAACCTTCAAGTGGCCACCTACTCCAAATTCCAACCTCA
  GAGCCCTGACTTCCTGGACATCACCAACCCCAAAGCCGTATTAGAAAACGCACTTAGGAACTTTGCCTGT
  CTGACCACCGGGGATGTGATTGCCATCAACTATAATGAAAAGATCTACGAACTGCGTGTGATGGAGACCA
  AACCCGACAAGGCAGTGTCCATCATTGAGTGTGACATGAACGTGGACTTTGATGCTCCCCTGGGCTACAA
  AGAACCCGAAAGACAAGTCCAGCATGAGGAGTCGACAGAAGGTGAAGCCGACCACAGTGGCTATGCTGGA
  GAGCTGGGCTTCCGCGCTTTCTCTGGATCTGGCAATAGACTGGATGGAAAGAAGAAAGGGGTAGAGCCCA
  GCCCCTCCCCAATCAAGCCTGGAGATATTAAAAGAGGAATTCCCAATTATGAATTTAAACTTGGTAAGAT
  AACTTTCATCAGAAATTCACGTCCCCTTGTCAAAAAGGTTGAAGAGGATGAAGCTGGAGGCAGATTCGTC
  GCTTTCTCTGGAGAAGGACAGTCATTGCGTAAAAAGGGAAGAAAGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213180 representing NM_001035247
  Red=Cloning site Green=Tags(s)
  
  MFSFNMFDHPIPRVFQNRFSTQYRCFSVSMLAGPNDRSDVEKGGKSRLNITYPMLFKLTNKNSDRMTHCG
  VLEFVADEGICYLPHWMMQNLLLEEGGLVQVESVNLQVATYSKFQPQSPDFLDITNPKAVLENALRNFAC
  LTTGDVIAINYNEKIYELRVMETKPDKAVSIIECDMNVDFDAPLGYKEPERQVQHEESTEGEADHSGYAG
  ELGFRAFSGSGNRLDGKKKGVEPSPSPIKPGDIKRGIPNYEFKLGKITFIRNSRPLVKKVEEDEAGGRFV
  AFSGEGQSLRKKGRKP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001035247
• ORF Size: 888 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001035247.1, NP_001030324.1
• RefSeq Size: 1501 bp
• RefSeq ORF: 801 bp
• Locus ID: 7353
• UniProt ID: Q92890
• Cytogenetics: 22q11.21
• MW: 33.32 kDa
• Gene Summary: The protein encoded by this gene forms a complex with two other proteins, nuclear protein localization-4 and valosin-containing protein, and this complex is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. A related pseudogene has been identified on chromosome 18. [provided by RefSeq, Jun 2009]