MLKL (NM_152649) Human Tagged ORF Clone
MLKL (Myc-DDK-tagged)-Human mixed lineage kinase domain-like (MLKL), transcript variant 1
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC213152 representing NM_152649
Red=Cloning site Blue=ORF Green=Tags(s)
>RC213152 representing NM_152649
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1413 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||2496 bp|
|RefSeq ORF||1416 bp|
|Protein Families||Druggable Genome, Protein Kinase|
|Gene Summary||This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]|
|cDNA Clone Resources|
|RC213152L1||Lenti ORF clone of Human mixed lineage kinase domain-like (MLKL), transcript variant 1, Myc-DDK-tagged||
|RC213152L2||Lenti ORF clone of Human mixed lineage kinase domain-like (MLKL), transcript variant 1, mGFP tagged||
|RC213152L3||Lenti ORF clone of Human mixed lineage kinase domain-like (MLKL), transcript variant 1, Myc-DDK-tagged||
|RC213152L4||Lenti ORF clone of Human mixed lineage kinase domain-like (MLKL), transcript variant 1, mGFP tagged||
|RG213152||MLKL (GFP-tagged) - Human mixed lineage kinase domain-like (MLKL), transcript variant 1||
|SC108101||MLKL (untagged)-Human mixed lineage kinase domain-like (MLKL), transcript variant 1||