Tubulin Polymerization Promoting Protein (TPPP) (NM_007030) Human Tagged ORF Clone

CAT#: RC213142

TPPP (Myc-DDK-tagged)-Human tubulin polymerization promoting protein (TPPP)

Product Images

Circular map for RC213142

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  Western blot validation of overexpression lysate (Cat# LY416249) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213142 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TPPP protein (Cat# TP313142). The protein was produced from HEK293T cells transfected with TPPP cDNA clone (Cat# RC213142) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tubulin Polymerization Promoting Protein
• Synonyms: p24; p25; p25alpha; TPPP/p25; TPPP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213142 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGACAAGGCCAAGCCTGCCAAAGCTGCCAACAGGACGCCCCCCAAGTCCCCGGGGGACCCCTCGA
  AGGACCGGGCAGCCAAGAGGCTGTCGCTGGAATCGGAGGGTGCTGGTGAGGGGGCAGCCGCATCCCCTGA
  GCTCAGTGCCCTGGAGGAGGCCTTCCGGCGCTTTGCCGTGCACGGGGACGCCAGGGCCACCGGGAGGGAG
  ATGCACGGCAAGAACTGGTCGAAGCTGTGCAAGGACTGCCAGGTGATCGACGGCAGGAACGTGACCGTCA
  CTGACGTGGACATCGTCTTCAGCAAGATCAAAGGGAAGTCTTGCCGGACCATCACCTTTGAGCAGTTCCA
  GGAGGCGCTGGAGGAGCTCGCCAAGAAGCGATTCAAAGACAAGAGCAGCGAGGAGGCCGTTCGCGAGGTG
  CACAGGCTCATCGAGGGCAAGGCGCCCATCATCTCAGGGGTGACGAAAGCCATCTCGTCGCCCACAGTGT
  CGAGGCTCACGGACACCACCAAGTTCACGGGCTCCCACAAGGAGCGCTTCGACCCCTCTGGCAAGGGCAA
  GGGCAAGGCTGGCCGCGTGGATCTGGTGGACGAGTCAGGCTATGTGTCCGGCTACAAGCACGCAGGCACC
  TACGACCAGAAGGTGCAAGGGGGCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213142 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADKAKPAKAANRTPPKSPGDPSKDRAAKRLSLESEGAGEGAAASPELSALEEAFRRFAVHGDARATGRE
  MHGKNWSKLCKDCQVIDGRNVTVTDVDIVFSKIKGKSCRTITFEQFQEALEELAKKRFKDKSSEEAVREV
  HRLIEGKAPIISGVTKAISSPTVSRLTDTTKFTGSHKERFDPSGKGKGKAGRVDLVDESGYVSGYKHAGT
  YDQKVQGGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007030
• ORF Size: 657 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007030.3
• RefSeq Size: 6039 bp
• RefSeq ORF: 660 bp
• Locus ID: 11076
• UniProt ID: O94811
• Cytogenetics: 5p15.33
• MW: 23.7 kDa
• Gene Summary: May play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown.[UniProtKB/Swiss-Prot Function]