OTUD7A (NM_130901) Human Tagged ORF Clone

CAT#: RC213015

OTUD7A (Myc-DDK-tagged)-Human OTU domain containing 7A (OTUD7A)

Product Images

Western blot validation of overexpression lysate (Cat# LY408886) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213015 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OTUD7A
• Synonyms: C15orf16; C16ORF15; CEZANNE2; OTUD7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213015 representing NM_130901
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTCTAGTGTGCTTCCAAACCCCACCTCGGCTGAGTGTTGGGCAGCACTTCTACATGATCCTATGA
  CTCTTGATATGGACGCAGTCCTGTCAGACTTTGTTCGGTCCACGGGGGCAGAACCTGGTCTGGCCAGAGA
  CCTGCTGGAAGGCAAAAACTGGGACCTGACAGCCGCTCTGAGCGACTATGAGCAGCTCCGCCAGGTGCAC
  ACAGCCAATCTGCCACATGTGTTCAATGAAGGGCGGGGTCCCAAGCAGCCAGAGCGAGAGCCACAGCCCG
  GGCACAAGGTGGAGCGACCCTGCCTGCAGAGGCAGGACGACATTGCCCAAGAAAAGCGGCTTTCCCGGGG
  GATTTCCCACGCCAGCTCAGCCATCGTCTCCCTGGCCCGGTCCCACGTGGCAAGTGAATGCAACAACGAG
  CAGTTCCCCCTGGAGATGCCAATCTACACATTCCAGTTGCCAGACCTGAGCGTGTACAGCGAGGATTTCA
  GGAGCTTCATCGAGCGGGACTTGATCGAGCAGGCAACAATGGTGGCTTTGGAGCAGGCAGGTCGCCTGAA
  CTGGTGGTCCACTGTGTGCACGAGCTGTAAACGGCTTCTTCCTCTGGCCACAACAGGGGATGGGAACTGC
  CTTTTACATGCTGCTTCACTGGGAATGTGGGGGTTTCACGACCGGGACCTGGTGTTACGGAAAGCTCTCT
  ATACCATGATGAGGACGGGAGCTGAGAGGGAAGCCCTGAAGCGGAGGTGGAGGTGGCAGCAGACGCAGCA
  GAATAAGGAGGAGGAGTGGGAGCGGGAGTGGACGGAGCTGCTGAAGCTGGCCTCCAGCGAGCCGCGCACA
  CACTTCAGCAAGAATGGCGGCACGGGCGGGGGTGTGGACAACTCTGAGGACCCCGTGTACGAGAGCCTGG
  AAGAGTTCCACGTTTTTGTCCTAGCCCATATATTAAGAAGGCCCATCGTTGTTGTGGCAGATACAATGTT
  AAGAGACTCAGGTGGAGAAGCGTTCGCACCCATCCCATTCGGAGGGATCTACCTGCCCTTGGAGGTCCCT
  CCCAACAGATGCCACTGCTCGCCTCTGGTTCTGGCCTATGATCAAGCCCATTTCTCTGCCCTTGTGTCCA
  TGGAACAGAGAGACCAGCAAAGAGAACAAGCCGTGATCCCCCTGACGGATTCTGAGCACAAGCTGCTGCC
  TCTGCACTTTGCAGTGGACCCTGGCAAGGACTGGGAGTGGGGGAAAGACGACAACGATAACGCCCGGCTG
  GCCCACCTTATCCTGTCGCTAGAAGCCAAGCTGAACCTTCTGCACAGCTACATGAACGTGACGTGGATCC
  GGATCCCCTCCGAGACACGGGCGCCCCTGGCACAGCCGGAGTCTCCCACGGCCTCGGCAGGGGAGGACGT
  GCAGTCCCTGGCCGACTCGCTGGACTCGGACCGCGATTCGGTGTGCAGCAATTCTAACAGCAATAACGGC
  AAGAACGGCAAGGACAAGGAGAAGGAGAAGCAGCGCAAGGAGAAGGACAAGACGCGCGCCGACTCCGTGG
  CCAACAAGCTGGGCAGCTTCAGCAAGACGCTGGGCATCAAGCTGAAGAAAAACATGGGCGGCCTCGGCGG
  CCTGGTGCACGGCAAGATGGGCCGCGCCAACTCCGCCAATGGCAAGAACGGGGACTCGGCCGAGCGGGGC
  AAGGAGAAGAAGGCCAAGTCGCGCAAGGGCAGCAAGGAGGAGTCTGGTGCGTCGGCCAGCACGTCGCCGT
  CGGAAAAGACCACGCCGTCGCCCACAGACAAGGCAGCGGGCGCGTCGCCGGCGGAGAAGGGCGGTGGGCC
  GCGGGGCGACGCCTGGAAGTACAGCACGGATGTGAAGCTGAGCCTCAACATCCTGCGCGCCGCCATGCAG
  GGGGAGCGCAAGTTCATCTTCGCCGGCCTGCTGCTCACCAGCCACCGGCACCAGTTCCACGAGGAGATGA
  TCGGCTACTACCTGACGAGCGCGCAGGAGCGCTTCAGCGCCGAGCAGGAGCAGCGGCGCCGCGACGCCGC
  TACTGCGGCCGCCGCTGCCGCCGCCGCCGCCGCCGCCACGGCCAAGCGGCCGCCGCGCAGACCGGAGACG
  GAGGGCGTGCCGGTCCCGGAGCGCGCCTCTCCGGGCCCACCCACGCAGCTGGTGCTCAAGCTCAAGGAGC
  GGCCGAGCCCCGGGCCCGCGGCAGGGCGTGCGGCGCGGGCGGCGGCGGGCGGCACGGCCTCCCCGGGGGG
  AGGCGCGCGGCGTGCGAGCGCCAGCGGACCAGTGCCTGGCCGCAGCCCCCCGGCGCCAGCGCGCCAGAGC
  GTCATCCACGTGCAGGCGTCGGGCGCGCGGGACGAGGCGTGCGCGCCGGCCGTGGGGGCGCTGCGGCCGT
  GCGCCACGTACCCGCAGCAGAACCGCTCGCTGTCGTCGCAGAGCTACAGCCCGGCGCGCGCCGCCGCCCT
  GCGCACCGTCAACACGGTCGAGTCGCTGGCGCGCGCGGTGCCCGGGGCCCTACCGGGCGCGGCGGGGACG
  GCGGGGGCGGCCGAGCACAAGTCGCAGACCTACACCAACGGCTTCGGCGCCCTGCGCGACGGCCTGGAGT
  TCGCCGACGCCGACGCGCCGACCGCGCGCTCGAACGGTGAGTGCGGCCGTGGCGGCCCGGGGCCGGTGCA
  GCGGCGCTGCCAGCGCGAGAACTGTGCGTTCTACGGGCGCGCCGAGACCGAGCACTACTGCTCCTACTGC
  TACCGCGAGGAGCTGCGGCGGCGGCGCGAGGCGCGCGGGGCCCGGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213015 representing NM_130901
  Red=Cloning site Green=Tags(s)
  
  MVSSVLPNPTSAECWAALLHDPMTLDMDAVLSDFVRSTGAEPGLARDLLEGKNWDLTAALSDYEQLRQVH
  TANLPHVFNEGRGPKQPEREPQPGHKVERPCLQRQDDIAQEKRLSRGISHASSAIVSLARSHVASECNNE
  QFPLEMPIYTFQLPDLSVYSEDFRSFIERDLIEQATMVALEQAGRLNWWSTVCTSCKRLLPLATTGDGNC
  LLHAASLGMWGFHDRDLVLRKALYTMMRTGAEREALKRRWRWQQTQQNKEEEWEREWTELLKLASSEPRT
  HFSKNGGTGGGVDNSEDPVYESLEEFHVFVLAHILRRPIVVVADTMLRDSGGEAFAPIPFGGIYLPLEVP
  PNRCHCSPLVLAYDQAHFSALVSMEQRDQQREQAVIPLTDSEHKLLPLHFAVDPGKDWEWGKDDNDNARL
  AHLILSLEAKLNLLHSYMNVTWIRIPSETRAPLAQPESPTASAGEDVQSLADSLDSDRDSVCSNSNSNNG
  KNGKDKEKEKQRKEKDKTRADSVANKLGSFSKTLGIKLKKNMGGLGGLVHGKMGRANSANGKNGDSAERG
  KEKKAKSRKGSKEESGASASTSPSEKTTPSPTDKAAGASPAEKGGGPRGDAWKYSTDVKLSLNILRAAMQ
  GERKFIFAGLLLTSHRHQFHEEMIGYYLTSAQERFSAEQEQRRRDAATAAAAAAAAAAATAKRPPRRPET
  EGVPVPERASPGPPTQLVLKLKERPSPGPAAGRAARAAAGGTASPGGGARRASASGPVPGRSPPAPARQS
  VIHVQASGARDEACAPAVGALRPCATYPQQNRSLSSQSYSPARAAALRTVNTVESLARAVPGALPGAAGT
  AGAAEHKSQTYTNGFGALRDGLEFADADAPTARSNGECGRGGPGPVQRRCQRENCAFYGRAETEHYCSYC
  YREELRRRREARGARP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130901
• ORF Size: 2778 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130901.3
• RefSeq Size: 3042 bp
• RefSeq ORF: 2781 bp
• Locus ID: 161725
• UniProt ID: Q8TE49
• Cytogenetics: 15q13.3
• Domains: zf-A20
• MW: 100.5 kDa
• Gene Summary: The protein encoded by this gene is a deubiquitinizing enzyme and possible tumor suppressor. The encoded protein acts on TNF receptor associated factor 6 (TRAF6) to control nuclear factor kappa B expression. However, this gene is downregulated by SNAIL1 in hepatocellular carcinoma cells, contributing to their progression and malignancy. [provided by RefSeq, Aug 2016]