TAF1 48 (TAF1A) (NM_005681) Human Tagged ORF Clone

CAT#: RC212971

TAF1A (Myc-DDK-tagged)-Human TATA box binding protein (TBP)-associated factor, RNA polymerase I, A, 48kDa (TAF1A), transcript variant 1

Product Images

Circular map for RC212971

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  Western blot validation of overexpression lysate (Cat# LY401732) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212971 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TAF1A protein (Cat# TP312971). The protein was produced from HEK293T cells transfected with TAF1A cDNA clone (Cat# RC212971) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TAF1 48
• Synonyms: MGC:17061; RAFI48; SL1; TAFI48
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212971 representing NM_005681
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGATTTCAGTGAAGAATTAAAAGGGCCTGTGACAGATGATGAAGAAGTGGAAACATCTGTGCTCA
  GTGGTGCAGGAATGCATTTTCCTTGGCTTCAAACATACGTAGAAACTGTGGCCATTGGAGGGAAAAGGAG
  GAAGGATTTTGCTCAGACAACAAGTGCTTGTTTAAGTTTTATCCAAGAAGCTCTGCTGAAGCACCAATGG
  CAGCAAGCTGCAGAATACATGTACAGTTATTTTCAGACCTTGGAAGATTCAGATAGCTACAAAAGGCAGG
  CTGCACCTGAGATTATTTGGAAGCTCGGAAGTGAAATTCTATTTTATCATCCCAAAAGCAACATGGAGAG
  TTTCAATACTTTTGCTAACCGGATGAAAAATATTGGCGTCATGAATTATTTAAAGATCTCCTTACAACAT
  GCATTATACCTTCTGCATCATGGAATGCTTAAAGATGCTAAGAGAAATCTGAGTGAGGCAGAGACATGGA
  GACATGGTGAAAATACGTCTTCCCGGGAAATATTAATCAACCTTATTCAGGCCTATAAAGGGCTTTTACA
  GTATTATACCTGGTCTGAAAAGAAGATGGAATTGTCAAAGCTTGATAAGGATGATTATGCTTACAATGCA
  GTAGCCCAGGATGTGTTCAACCACAGCTGGAAGACATCTGCAAATATTTCTGCATTGATTAAAATTCCTG
  GAGTTTGGGACCCTTTTGTGAAGAGTTATGTAGAAATGCTGGAATTCTATGGGGATCGAGATGGAGCCCA
  AGAGGTACTCACCAATTATGCATATGATGAAAAGTTTCCATCAAATCCAAATGCCCATATCTACTTATAC
  AACTTTCTAAAGAGACAGAAGGCACCAAGATCAAAATTGATAAGTGTGCTTAAGATTTTGTATCAGATTG
  TACCATCTCATAAATTGATGTTGGAATTCCATACATTACTTAGAAAATCAGAAAAAGAAGAACACCGTAA
  ACTGGGGTTGGAGGTATTATTTGGAGTCTTAGATTTTGCCGGATGCACTAAGAATATAACTGCTTGGAAA
  TACTTGGCAAAATATCTGAAAAATATCTTAATGGGAAACCACCTTGCGTGGGTTCAAGAAGAGTGGAACT
  CCAGGAAAAACTGGTGGCCAGGGTTTCATTTCAGCTACTTTTGGGCAAAAAGTGATTGGAAGGAAGATAC
  AGCTTTGGCCTGTGAGAAAGCTTTTGTGGCTGGTTTACTGTTAGGAAAAGGTTGTAGATATTTCCGGTAT
  ATTTTAAAGCAAGATCACCAAATCTTAGGGAAGAAAATTAAGCGGATGAAGAGATCTGTGAAAAAATACA
  GTATTGTAAATCCAAGACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212971 representing NM_005681
  Red=Cloning site Green=Tags(s)
  
  MSDFSEELKGPVTDDEEVETSVLSGAGMHFPWLQTYVETVAIGGKRRKDFAQTTSACLSFIQEALLKHQW
  QQAAEYMYSYFQTLEDSDSYKRQAAPEIIWKLGSEILFYHPKSNMESFNTFANRMKNIGVMNYLKISLQH
  ALYLLHHGMLKDAKRNLSEAETWRHGENTSSREILINLIQAYKGLLQYYTWSEKKMELSKLDKDDYAYNA
  VAQDVFNHSWKTSANISALIKIPGVWDPFVKSYVEMLEFYGDRDGAQEVLTNYAYDEKFPSNPNAHIYLY
  NFLKRQKAPRSKLISVLKILYQIVPSHKLMLEFHTLLRKSEKEEHRKLGLEVLFGVLDFAGCTKNITAWK
  YLAKYLKNILMGNHLAWVQEEWNSRKNWWPGFHFSYFWAKSDWKEDTALACEKAFVAGLLLGKGCRYFRY
  ILKQDHQILGKKIKRMKRSVKKYSIVNPRL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005681
• ORF Size: 1350 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005681.1
• RefSeq Size: 1893 bp
• RefSeq ORF: 1353 bp
• Locus ID: 9015
• UniProt ID: Q15573
• Cytogenetics: 1q41
• Protein Families: Transcription Factors
• MW: 52.5 kDa
• Gene Summary: This gene encodes a subunit of the RNA polymerase I complex, Selectivity Factor I (SLI). The encoded protein is a TATA box-binding protein-associated factor that plays a role in the assembly of the RNA polymerase I preinitiation complex. Alternate splicing results in multiple transcript variants encoding multiple isoforms.[provided by RefSeq, Jan 2011]