SLC6A19 (NM_001003841) Human Tagged ORF Clone

CAT#: RC212948

SLC6A19 (Myc-DDK-tagged)-Human solute carrier family 6 (neutral amino acid transporter), member 19 (SLC6A19)

Product Images

Circular map for RC212948

• 

  Western blot validation of overexpression lysate (Cat# LY424027) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212948 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC6A19
• Synonyms: B0AT1; HND
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212948 representing NM_001003841
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAGGCTCGTGCTGCCCAACCCCGGCCTAGACGCCCGGATCCCGTCCCTGGCTGAGCTGGAGACCA
  TCGAGCAGGAGGAGGCCAGCTCCCGGCCGAAGTGGGACAACAAGGCGCAGTACATGCTCACCTGCCTGGG
  CTTCTGCGTGGGCCTCGGCAACGTGTGGCGCTTCCCCTACCTGTGTCAGAGCCACGGAGGAGGAGCCTTC
  ATGATCCCGTTCCTCATCCTGCTGGTCCTGGAGGGCATCCCCCTGCTGTACCTGGAGTTCGCCATCGGGC
  AGCGGCTGCGGCGGGGCAGCCTGGGTGTGTGGAGCTCCATCCACCCGGCCCTGAAGGGCCTAGGCCTGGC
  CTCCATGCTCACGTCCTTCATGGTGGGACTGTATTACAACACCATCATCTCCTGGATCATGTGGTACTTA
  TTCAACTCCTTCCAGGAGCCTCTGCCCTGGAGCGACTGCCCGCTCAACGAGAACCAGACAGGGTATGTGG
  ACGAGTGCGCCAGGAGCTCCCCTGTGGACTACTTCTGGTACCGAGAGACGCTCAACATCTCCACGTCCAT
  CAGCGACTCGGGCTCCATCCAGTGGTGGATGCTGCTGTGCCTGGCCTGCGCATGGAGCGTCCTGTACATG
  TGCACCATCCGCGGCATCGAGACCACCGGGAAGGCCGTGTACATCACCTCCACGCTGCCCTATGTCGTCC
  TGACCATCTTCCTCATCCGAGGCCTGACGCTGAAGGGCGCCACCAATGGCATCGTCTTCCTCTTCACGCC
  CAACGTCACGGAGCTGGCCCAGCCGGACACCTGGCTGGACGCGGGCGCACAGGTCTTCTTCTCCTTCTCC
  CTGGCCTTCGGGGGCCTCATCTCCTTCTCCAGCTACAACTCTGTGCACAACAACTGCGAGAAGGACTCGG
  TGATTGTGTCCATCATCAACGGCTTCACATCGGTGTATGTGGCCATCGTGGTCTACTCCGTCATTGGGTT
  CCGCGCCACGCAGCGCTACGACGACTGCTTCAGCACGAACATCCTGACCCTCATCAACGGGTTCGACCTG
  CCTGAAGGCAACGTGACCCAGGAGAACTTTGTGGACATGCAGCAGCGGTGCAACGCCTCCGACCCCGCGG
  CCTACGCGCAGCTGGTGTTCCAGACCTGCGACATCAACGCCTTCCTCTCAGAGGCCGTGGAGGGCACAGG
  CCTGGCCTTCATCGTCTTCACCGAGGCCATCACCAAGATGCCGTTGTCCCCACTGTGGTCTGTGCTCTTC
  TTCATTATGCTCTTCTGCCTGGGGCTGTCATCTATGTTTGGGAACATGGAGGGCGTCGTTGTGCCCCTGC
  AGGACCTCAGAGTCATCCCCCCGAAGTGGCCCAAGGAGGTGCTCACAGGCCTCATCTGCCTGGGGACATT
  CCTCATTGGCTTCATCTTCACGCTGAACTCCGGCCAGTACTGGCTCTCCCTGCTGGACAGCTATGCCGGC
  TCCATTCCCCTGCTCATCATCGCCTTCTGCGAGATGTTCTCTGTGGTCTACGTGTACGGTGTGGACAGGT
  TCAATAAGGACATCGAGTTCATGATCGGCCACAAGCCCAACATCTTCTGGCAAGTCACGTGGCGCGTGGT
  CAGCCCCCTGCTCATGCTGATCATCTTCCTCTTCTTCTTCGTGGTAGAGGTCAGTCAGGAGCTGACCTAC
  AGCATCTGGGACCCTGGCTACGAGGAATTTCCCAAATCCCAGAAGATCTCCTACCCGAACTGGGTGTATG
  TGGTGGTGGTGATTGTGGCTGGAGTGCCCTCCCTCACCATCCCTGGCTATGCCATCTACAAGCTCATCAG
  GAACCACTGCCAGAAGCCAGGGGACCATCAGGGGCTGGTGAGCACACTGTCCACAGCCTCCATGAACGGG
  GACCTGAAGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212948 representing NM_001003841
  Red=Cloning site Green=Tags(s)
  
  MVRLVLPNPGLDARIPSLAELETIEQEEASSRPKWDNKAQYMLTCLGFCVGLGNVWRFPYLCQSHGGGAF
  MIPFLILLVLEGIPLLYLEFAIGQRLRRGSLGVWSSIHPALKGLGLASMLTSFMVGLYYNTIISWIMWYL
  FNSFQEPLPWSDCPLNENQTGYVDECARSSPVDYFWYRETLNISTSISDSGSIQWWMLLCLACAWSVLYM
  CTIRGIETTGKAVYITSTLPYVVLTIFLIRGLTLKGATNGIVFLFTPNVTELAQPDTWLDAGAQVFFSFS
  LAFGGLISFSSYNSVHNNCEKDSVIVSIINGFTSVYVAIVVYSVIGFRATQRYDDCFSTNILTLINGFDL
  PEGNVTQENFVDMQQRCNASDPAAYAQLVFQTCDINAFLSEAVEGTGLAFIVFTEAITKMPLSPLWSVLF
  FIMLFCLGLSSMFGNMEGVVVPLQDLRVIPPKWPKEVLTGLICLGTFLIGFIFTLNSGQYWLSLLDSYAG
  SIPLLIIAFCEMFSVVYVYGVDRFNKDIEFMIGHKPNIFWQVTWRVVSPLLMLIIFLFFFVVEVSQELTY
  SIWDPGYEEFPKSQKISYPNWVYVVVVIVAGVPSLTIPGYAIYKLIRNHCQKPGDHQGLVSTLSTASMNG
  DLKY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001003841
• ORF Size: 1902 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003841.1, NP_001003841.1
• RefSeq Size: 1905 bp
• RefSeq ORF: 1905 bp
• Locus ID: 340024
• UniProt ID: Q695T7
• Cytogenetics: 5p15.33
• Protein Families: Druggable Genome, Transmembrane
• MW: 70.9 kDa
• Gene Summary: This gene encodes a system B(0) transmembrane protein that actively transports most neutral amino acids across the apical membrane of epithelial cells. Mutations in this gene may result in Hartnup disorder, an inherited disease with symptoms such as pellagra, cerebellar ataxia, and psychosis. The expression and function of B0AT1 (SLC6A19) in intestinal cells depends on the presence of the accessory protein angiotensin-converting enzyme 2 (ACE2) which, among other functions, acts as a chaperone for membrane trafficking of B0AT1. The ACE2 is also the cellular receptor for severe acute respiratory syndrome-coronavirus (SARS-CoV) and for SARS-CoV-2 that is causing the coronavirus 2019 (COVID-19) pandemic [provided by RefSeq, Jul 2020]