AGXT (NM_000030) Human Tagged ORF Clone

CAT#: RC212899

AGXT (Myc-DDK-tagged)-Human alanine-glyoxylate aminotransferase (AGXT)

Product Images

Circular map for RC212899

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY AGXT (Cat# RC212899, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AGXT antibody(Cat# TA890098). Positive lysates LY424964 (100ug) and LC424964 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY424964) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212899 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified AGXT protein (Cat# TP312899). The protein was produced from HEK293T cells transfected with AGXT cDNA clone (Cat# RC212899) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AGXT
• Synonyms: AGT; AGT1; AGXT1; PH1; SPAT; SPT; TLH6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212899 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCTCACAAGCTGCTGGTGACCCCCCCCAAGGCCCTGCTCAAGCCCCTCTCCATCCCCAACCAGC
  TCCTGCTGGGGCCTGGTCCTTCCAACCTGCCTCCTCGCATCATGGCAGCCGGGGGGCTGCAGATGATCGG
  GTCCATGAGCAAGGATATGTACCAGATCATGGACGAGATCAAGGAAGGCATCCAGTACGTGTTCCAGACC
  AGGAACCCACTCACACTGGTCATCTCTGGCTCGGGACACTGTGCCCTGGAGGCCGCCCTGGTCAATGTGC
  TGGAGCCTGGGGACTCCTTCCTGGTTGGGGCCAATGGCATTTGGGGGCAGCGAGCCGTGGACATCGGGGA
  GCGCATAGGAGCCCGAGTGCACCCGATGACCAAGGACCCTGGAGGCCACTACACACTGCAGGAGGTGGAG
  GAGGGCCTGGCCCAGCACAAGCCAGTGCTGCTGTTCTTAACCCACGGGGAGTCGTCCACCGGCGTGCTGC
  AGCCCCTTGATGGCTTCGGGGAACTCTGCCACAGGTACAAGTGCCTGCTCCTGGTGGATTCGGTGGCATC
  CCTGGGCGGGACCCCCCTTTACATGGACCGGCAAGGCATCGACATCCTGTACTCGGGCTCCCAGAAGGCC
  CTGAACGCCCCTCCAGGGACCTCACTCATCTCCTTCAGTGACAAGGCCAAAAAGAAGATGTACTCCCGCA
  AGACGAAGCCCTTCTCCTTCTACCTGGACATCAAGTGGCTGGCCAACTTCTGGGGCTGTGACGACCAGCC
  CAGGATGTACCATCACACAATCCCCGTCATCAGCCTGTACAGCCTGAGAGAGAGCCTGGCCCTCATTGCG
  GAACAGGGCCTGGAGAACAGCTGGCGCCAGCACCGCGAGGCCGCGGCGTATCTGCATGGGCGCCTGCAGG
  CACTGGGGCTGCAGCTCTTCGTGAAGGACCCGGCGCTCCGGCTTCCCACAGTCACCACTGTGGCTGTACC
  CGCTGGCTATGACTGGAGAGACATCGTCAGCTACGTCATAGACCACTTCGACATTGAGATCATGGGTGGC
  CTTGGGCCCTCCACGGGGAAGGTGCTGCGGATCGGCCTGCTGGGCTGCAATGCCACCCGCGAGAATGTGG
  ACCGCGTGACGGAGGCCCTGAGGGCGGCCCTGCAGCACTGCCCCAAGAAGAAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212899 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASHKLLVTPPKALLKPLSIPNQLLLGPGPSNLPPRIMAAGGLQMIGSMSKDMYQIMDEIKEGIQYVFQT
  RNPLTLVISGSGHCALEAALVNVLEPGDSFLVGANGIWGQRAVDIGERIGARVHPMTKDPGGHYTLQEVE
  EGLAQHKPVLLFLTHGESSTGVLQPLDGFGELCHRYKCLLLVDSVASLGGTPLYMDRQGIDILYSGSQKA
  LNAPPGTSLISFSDKAKKKMYSRKTKPFSFYLDIKWLANFWGCDDQPRMYHHTIPVISLYSLRESLALIA
  EQGLENSWRQHREAAAYLHGRLQALGLQLFVKDPALRLPTVTTVAVPAGYDWRDIVSYVIDHFDIEIMGG
  LGPSTGKVLRIGLLGCNATRENVDRVTEALRAALQHCPKKKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000030
• ORF Size: 1176 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000030.3
• RefSeq Size: 1611 bp
• RefSeq ORF: 1179 bp
• Locus ID: 189
• UniProt ID: P21549
• Cytogenetics: 2q37.3
• Domains: aminotran_5
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Glycine, serine and threonine metabolism, Metabolic pathways
• MW: 43 kDa
• Gene Summary: This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification. Mutations in this gene, some of which alter subcellular targetting, have been associated with type I primary hyperoxaluria. [provided by RefSeq, Jul 2008]