RUNX2 (NM_001024630) Human Tagged ORF Clone
RUNX2 (Myc-DDK-tagged)-Human runt-related transcription factor 2 (RUNX2), transcript variant 1
|Type||Human Tagged ORF Clone|
|Synonyms||AML3; CBF-alpha-1; CBFA1; CCD; CCD1; CLCD; OSF-2; OSF2; PEA2aA; PEBP2aA|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC212884 representing NM_001024630
Red=Cloning site Blue=ORF Green=Tags(s)
>RC212884 representing NM_001024630
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1767 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||5572 bp|
|RefSeq ORF||1566 bp|
|Protein Families||Druggable Genome, Transcription Factors|
|Gene Summary||This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Two regions of potential trinucleotide repeat expansions are present in the N-terminal region of the encoded protein, and these and other mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2016]|
|cDNA Clone Resources|
|RC212884L1||Lenti ORF clone of Human runt-related transcription factor 2 (RUNX2), transcript variant 1, Myc-DDK-tagged||
|RC212884L2||Lenti ORF clone of Human runt-related transcription factor 2 (RUNX2), transcript variant 1, mGFP tagged||
|RC212884L3||Lenti ORF clone of Human runt-related transcription factor 2 (RUNX2), transcript variant 1, Myc-DDK-tagged||
|RC212884L4||Lenti ORF clone of Human runt-related transcription factor 2 (RUNX2), transcript variant 1, mGFP tagged||
|RG212884||RUNX2 (GFP-tagged) - Human runt-related transcription factor 2 (RUNX2), transcript variant 1||
|SC302270||RUNX2 (untagged)-Human runt-related transcription factor 2 (RUNX2), transcript variant 1||
|SC318014||RUNX2 (untagged)-Human runt-related transcription factor 2 (RUNX2), transcript variant 1||