Keratin 80 (KRT80) (NM_182507) Human Tagged ORF Clone

CAT#: RC212859

KRT80 (Myc-DDK-tagged)-Human keratin 80 (KRT80), transcript variant 1

Product Images

Circular map for RC212859

• 

  Western blot validation of overexpression lysate (Cat# LY405527) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212859 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Keratin 80
• Synonyms: KB20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212859 representing NM_182507
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTGCCGCTCCTGCGTGGTTGGCTTCAGCAGCCTCAGCAGCTGTGAGGTGACCCCGGTGGGCAGCC
  CCCGGCCTGGAACCTCAGGATGGGACAGCTGCAGGGCCCCCGGGCCGGGCTTCAGCTCCCGCAGCCTCAC
  AGGCTGCTGGTCGGCTGGCACTATCTCCAAGGTGACTGTGAACCCCGGCCTGCTGGTGCCCCTGGATGTC
  AAGTTGGACCCCGCTGTTCAGCAGCTGAAGAACCAGGAGAAGGAGGAGATGAAGGCCCTCAATGATAAAT
  TTGCCTCCCTAATTGGCAAGGTGCAAGCCCTGGAACAGCGCAACCAGCTGCTGGAGACACGCTGGAGCTT
  CCTGCAGGGCCAGGACTCAGCCATCTTCGACCTCGGGCATCTCTATGAGGAATATCAGGGCCGGCTGCAG
  GAGGAACTGCGCAAAGTGAGCCAGGAGCGGGGGCAGCTGGAGGCCAACCTGCTGCAGGTGCTGGAGAAGG
  TTGAGGAGTTTCGAATCAGGTATGAGGATGAGATCTCCAAGCGCACAGACATGGAGTTCACCTTTGTTCA
  GCTGAAGAAGGACCTGGATGCAGAGTGTCTTCATCGGACTGAACTGGAAACCAAGTTAAAAAGCCTGGAG
  AGCTTCGTGGAGTTGATGAAAACCATCTATGAGCAGGAGCTGAAGGACCTGGCAGCACAGGTGAAGGATG
  TGTCGGTGACCGTCGGCATGGACAGCCGCTGCCACATCGACCTGAGCGGCATCGTGGAGGAGGTGAAGGC
  CCAGTATGACGCCGTCGCGGCTCGCAGCCTGGAGGAGGCCGAGGCATACTCTCGGAGCCAGCTGGAGGAG
  CAGGCCGCCCGCTCGGCCGAGTATGGGAGCAGCCTCCAGAGCAGCCGCAGCGAGATCGCGGATCTCAATG
  TGCGCATCCAGAAGCTGCGGTCCCAGATCCTCTCTGTCAAGAGCCATTGCCTGAAACTGGAGGAGAACAT
  CAAGACAGCTGAGGAGCAGGGTGAGCTGGCCTTCCAGGATGCCAAGACCAAGCTGGCCCAGCTGGAGGCC
  GCCCTGCAGCAGGCCAAGCAGGACATGGCGCGGCAGCTGCGCAAGTACCAGGAGCTGATGAACGTCAAGC
  TGGCCCTGGACATCGAGATCGCCACCTACAGGAAGCTGGTGGAGGGCGAGGAGGGCAGGATGGACTCGCC
  CTCAGCCACTGTGGTCAGCGCTGTGCAGTCCAGGTGCAAAACCGCTGCCTCCAGATCAGGCCTCTCCAAG
  GCCCCCTCCCGAAAGAAGAAGGGCAGCAAAGGCCCCGTGATCAAAATCACCGAAATGTCAGAGAAGTACT
  TCTCGCAGGAGTCGGAGGTCTCAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212859 representing NM_182507
  Red=Cloning site Green=Tags(s)
  
  MACRSCVVGFSSLSSCEVTPVGSPRPGTSGWDSCRAPGPGFSSRSLTGCWSAGTISKVTVNPGLLVPLDV
  KLDPAVQQLKNQEKEEMKALNDKFASLIGKVQALEQRNQLLETRWSFLQGQDSAIFDLGHLYEEYQGRLQ
  EELRKVSQERGQLEANLLQVLEKVEEFRIRYEDEISKRTDMEFTFVQLKKDLDAECLHRTELETKLKSLE
  SFVELMKTIYEQELKDLAAQVKDVSVTVGMDSRCHIDLSGIVEEVKAQYDAVAARSLEEAEAYSRSQLEE
  QAARSAEYGSSLQSSRSEIADLNVRIQKLRSQILSVKSHCLKLEENIKTAEEQGELAFQDAKTKLAQLEA
  ALQQAKQDMARQLRKYQELMNVKLALDIEIATYRKLVEGEEGRMDSPSATVVSAVQSRCKTAASRSGLSK
  APSRKKKGSKGPVIKITEMSEKYFSQESEVSE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182507
• ORF Size: 1356 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182507.3
• RefSeq Size: 3411 bp
• RefSeq ORF: 1359 bp
• Locus ID: 144501
• UniProt ID: Q6KB66
• Cytogenetics: 12q13.13
• MW: 50.3 kDa
• Gene Summary: Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. This gene's expression profile shows that it encodes a type II epithelial keratin, although structurally the encoded protein is more like a type II hair keratin. This protein is involved in cell differentiation, localizing near desmosomal plaques in earlier stages of differentiation but then dispersing throughout the cytoplasm in terminally differentiating cells. The type II keratins are clustered in a region of chromosome 12q13. Two transcript variants encoding two different fully functional isoforms have been found for this gene.[provided by RefSeq, Oct 2010]