RON (MST1R) (NM_002447) Human Tagged Lenti ORF Clone

CAT#: RC212786L3

  • LentiORF®

Lenti ORF clone of Human macrophage stimulating 1 receptor (c-met-related tyrosine kinase) (MST1R), Myc-DDK-tagged


  "NM_002447" in other vectors (7)

Reconstitution Protocol

USD 1,498.00

3 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (5)
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Reverse primer to sequence targets cloned in expression vectors.
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MST1R mouse monoclonal antibody,clone OTI1D12
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol RON
Synonyms CD136; CDw136; NPCA3; PTK8; RON; SEA
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
The ORF insert of this clone is exactly the same as(RC212786).
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002447
ORF Size 4203 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002447.1
RefSeq Size 4541 bp
RefSeq ORF 4203 bp
Locus ID 4486
UniProt ID Q04912
Cytogenetics 3p21.31
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transmembrane
MW 152.37 kDa
Gene Summary This gene encodes a cell surface receptor for macrophage-stimulating protein (MSP) with tyrosine kinase activity. The mature form of this protein is a heterodimer of disulfide-linked alpha and beta subunits, generated by proteolytic cleavage of a single-chain precursor. The beta subunit undergoes tyrosine phosphorylation upon stimulation by MSP. This protein is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung, and together with MSP, thought to be involved in host defense. Alternative splicing generates multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Jan 2016]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.