RON (MST1R) (NM_002447) Human Tagged ORF Clone

CAT#: RC212786

MST1R (Myc-DDK-tagged)-Human macrophage stimulating 1 receptor (c-met-related tyrosine kinase) (MST1R)

Product Images

Circular map for RC212786

• 

  Western blot validation of overexpression lysate (Cat# LY419321) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212786 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MST1R (Cat# RC212786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MST1R (Cat# TA812779)(1:500). Positive lysates LY419321 (100ug) and LC419321 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RON
• Synonyms: CD136; CDw136; NPCA3; PTK8; RON; SEA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212786 representing NM_002447
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTCCTCCCGCCGCTGCCTCAGTCCTTCCTGTTGCTGCTGCTGTTGCCTGCCAAGCCCGCGGCGG
  GCGAGGACTGGCAGTGCCCGCGCACCCCCTACGCGGCCTCTCGCGACTTTGACGTGAAGTACGTGGTGCC
  CAGCTTCTCCGCCGGAGGCCTGGTACAGGCCATGGTGACCTACGAGGGCGACAGAAATGAGAGTGCTGTG
  TTTGTAGCCATACGCAATCGCCTGCATGTGCTTGGGCCTGACCTGAAGTCTGTCCAGAGCCTGGCCACGG
  GCCCTGCTGGAGACCCTGGCTGCCAGACGTGTGCAGCCTGTGGCCCAGGACCCCACGGCCCTCCCGGTGA
  CACAGACACAAAGGTGCTGGTGCTGGATCCCGCGCTGCCTGCGCTGGTCAGTTGTGGCTCCAGCCTGCAG
  GGCCGCTGCTTCCTGCATGACCTAGAGCCCCAAGGGACAGCCGTGCATCTGGCAGCGCCAGCCTGCCTCT
  TCTCAGCCCACCATAACCGGCCCGATGACTGCCCCGACTGTGTGGCCAGCCCATTGGGCACCCGTGTAAC
  TGTGGTTGAGCAAGGCCAGGCCTCCTATTTCTACGTGGCATCCTCACTGGACGCAGCCGTGGCTGCCAGC
  TTCAGCCCACGCTCAGTGTCTATCAGGCGTCTCAAGGCTGACGCCTCGGGATTCGCACCGGGCTTTGTGG
  CGTTGTCAGTGCTGCCCAAGCATCTTGTCTCCTACAGTATTGAATACGTGCACAGCTTCCACACGGGAGC
  CTTCGTGTACTTCCTGACTGTACAGCCGGCCAGCGTGACAGATGATCCTAGTGCCCTGCACACACGCCTG
  GCACGGCTTAGCGCCACTGAGCCAGAGTTGGGTGACTATCGGGAGCTGGTCCTCGACTGCAGATTTGCTC
  CAAAACGCAGGCGCCGGGGGGCCCCAGAAGGCGGACAGCCCTACCCTGTGCTGCGGGTGGCCCACTCCGC
  TCCAGTGGGTGCCCAACTTGCCACTGAGCTGAGCATCGCCGAGGGCCAGGAAGTACTATTTGGGGTCTTT
  GTGACTGGCAAGGATGGTGGTCCTGGCGTGGGCCCCAACTCTGTCGTCTGTGCCTTCCCCATTGACCTGC
  TGGACACACTAATTGATGAGGGTGTGGAGCGCTGTTGTGAATCCCCAGTCCATCCAGGCCTCCGGCGAGG
  CCTCGACTTCTTCCAGTCGCCCAGTTTTTGCCCCAACCCGCCTGGCCTGGAAGCCCTCAGCCCCAACACC
  AGCTGCCGCCACTTCCCTCTGCTGGTCAGTAGCAGCTTCTCACGTGTGGACCTATTCAATGGGCTGTTGG
  GACCAGTACAGGTCACTGCATTGTATGTGACACGCCTTGACAACGTCACAGTGGCACACATGGGCACAAT
  GGATGGGCGTATCCTGCAGGTGGAGCTGGTCAGGTCACTAAACTACTTGCTGTATGTGTCCAACTTCTCA
  CTGGGTGACAGTGGGCAGCCCGTGCAGCGGGATGTCAGTCGTCTTGGGGACCACCTACTCTTTGCCTCTG
  GGGACCAGGTTTTCCAGGTACCTATCCAAGGCCCTGGCTGCCGCCACTTCCTGACCTGTGGGCGTTGCCT
  AAGGGCATGGCATTTCATGGGCTGTGGCTGGTGTGGGAACATGTGCGGCCAGCAGAAGGAGTGTCCTGGC
  TCCTGGCAACAGGACCACTGCCCACCTAAGCTTACTGAGTTCCACCCCCACAGTGGACCTCTAAGGGGCA
  GTACAAGGCTGACCCTGTGTGGCTCCAACTTCTACCTTCACCCTTCTGGTCTGGTGCCTGAGGGAACCCA
  TCAGGTCACTGTGGGCCAAAGTCCCTGCCGGCCACTGCCCAAGGACAGCTCAAAACTCAGACCAGTGCCC
  CGGAAAGACTTTGTAGAGGAGTTTGAGTGTGAACTGGAGCCCTTGGGCACCCAGGCAGTGGGGCCTACCA
  ACGTCAGCCTCACCGTGACTAACATGCCACCGGGCAAGCACTTCCGGGTGGACGGCACCTCCGTGCTGAG
  AGGCTTCTCTTTCATGGAGCCAGTGCTGATAGCAGTGCAACCCCTCTTTGGCCCACGGGCAGGAGGCACC
  TGTCTCACTCTTGAAGGCCAGAGTCTGTCTGTAGGCACCAGCCGGGCTGTGCTGGTCAATGGGACTGAGT
  GTCTGCTAGCACGGGTCAGTGAGGGGCAGCTTTTATGTGCCACACCCCCTGGGGCCACGGTGGCCAGTGT
  CCCCCTTAGCCTGCAGGTGGGGGGTGCCCAGGTACCTGGTTCCTGGACCTTCCAGTACAGAGAAGACCCT
  GTCGTGCTAAGCATCAGCCCCAACTGTGGCTACATCAACTCCCACATCACCATCTGTGGCCAGCATCTAA
  CTTCAGCATGGCACTTAGTGCTGTCATTCCATGACGGGCTTAGGGCAGTGGAAAGCAGGCAGTGTGAGAG
  GCAGCTTCCAGAGCAGCAGCTGTGCCGCCTTCCTGAATATGTGGTCCGAGACCCCCAGGGATGGGTGGCA
  GGGAATCTGAGTGCCCGAGGGGATGGAGCTGCTGGCTTTACACTGCCTGGCTTTCGCTTCCTACCCCCAC
  CCCATCCACCCAGTGCCAACCTAGTTCCACTGAAGCCTGAGGAGCATGCCATTAAGTTTGAGTATATTGG
  GCTGGGCGCTGTGGCTGACTGTGTGGGTATCAACGTGACCGTGGGTGGTGAGAGCTGCCAGCACGAGTTC
  CGGGGGGACATGGTTGTCTGCCCCCTGCCCCCATCCCTGCAGCTTGGCCAGGATGGTGCCCCATTGCAGG
  TCTGCGTAGATGGTGAATGTCATATCCTGGGTAGAGTGGTGCGGCCAGGGCCAGATGGGGTCCCACAGAG
  CACGCTCCTTGGTATCCTGCTGCCTTTGCTGCTGCTTGTGGCTGCACTGGCGACTGCACTGGTCTTCAGC
  TACTGGTGGCGGAGGAAGCAGCTAGTTCTTCCTCCCAACCTGAATGACCTGGCATCCCTGGACCAGACTG
  CTGGAGCCACACCCCTGCCTATTCTGTACTCGGGCTCTGACTACAGAAGTGGCCTTGCACTCCCTGCCAT
  TGATGGTCTGGATTCCACCACTTGTGTCCATGGAGCATCCTTCTCCGATAGTGAAGATGAATCCTGTGTG
  CCACTGCTGCGGAAAGAGTCCATCCAGCTAAGGGACCTGGACTCTGCGCTCTTGGCTGAGGTCAAGGATG
  TGCTGATTCCCCATGAGCGGGTGGTCACCCACAGTGACCGAGTCATTGGCAAAGGCCACTTTGGAGTTGT
  CTACCACGGAGAATACATAGACCAGGCCCAGAATCGAATCCAATGTGCCATCAAGTCACTAAGTCGCATC
  ACAGAGATGCAGCAGGTGGAGGCCTTCCTGCGAGAGGGGCTGCTCATGCGTGGCCTGAACCACCCGAATG
  TGCTGGCTCTCATTGGTATCATGTTGCCACCTGAGGGCCTGCCCCATGTGCTGCTGCCCTATATGTGCCA
  CGGTGACCTGCTCCAGTTCATCCGCTCACCTCAGCGGAACCCCACCGTGAAGGACCTCATCAGCTTTGGC
  CTGCAGGTAGCCCGCGGCATGGAGTACCTGGCAGAGCAGAAGTTTGTGCACAGGGACCTGGCTGCGCGGA
  ACTGCATGCTGGACGAGTCATTCACAGTCAAGGTGGCTGACTTTGGTTTGGCCCGCGACATCCTGGACAG
  GGAGTACTATAGTGTTCAACAGCATCGCCACGCTCGCCTACCTGTGAAGTGGATGGCGCTGGAGAGCCTG
  CAGACCTATAGATTTACCACCAAGTCTGATGTGTGGTCATTTGGTGTGCTGCTGTGGGAACTGCTGACAC
  GGGGTGCCCCACCATACCGCCACATTGACCCTTTTGACCTTACCCACTTCCTGGCCCAGGGTCGGCGCCT
  GCCCCAGCCTGAGTATTGCCCTGATTCTCTGTACCAAGTGATGCAGCAATGCTGGGAGGCAGACCCAGCA
  GTGCGACCCACCTTCAGAGTACTAGTGGGGGAGGTGGAGCAGATAGTGTCTGCACTGCTTGGGGACCATT
  ATGTGCAGCTGCCAGCAACCTACATGAACTTGGGCCCCAGCACCTCGCATGAGATGAATGTGCGTCCAGA
  ACAGCCGCAGTTCTCACCCATGCCAGGGAATGTACGCCGGCCCCGGCCACTCTCAGAGCCTCCTCGGCCC
  ACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212786 representing NM_002447
  Red=Cloning site Green=Tags(s)
  
  MELLPPLPQSFLLLLLLPAKPAAGEDWQCPRTPYAASRDFDVKYVVPSFSAGGLVQAMVTYEGDRNESAV
  FVAIRNRLHVLGPDLKSVQSLATGPAGDPGCQTCAACGPGPHGPPGDTDTKVLVLDPALPALVSCGSSLQ
  GRCFLHDLEPQGTAVHLAAPACLFSAHHNRPDDCPDCVASPLGTRVTVVEQGQASYFYVASSLDAAVAAS
  FSPRSVSIRRLKADASGFAPGFVALSVLPKHLVSYSIEYVHSFHTGAFVYFLTVQPASVTDDPSALHTRL
  ARLSATEPELGDYRELVLDCRFAPKRRRRGAPEGGQPYPVLRVAHSAPVGAQLATELSIAEGQEVLFGVF
  VTGKDGGPGVGPNSVVCAFPIDLLDTLIDEGVERCCESPVHPGLRRGLDFFQSPSFCPNPPGLEALSPNT
  SCRHFPLLVSSSFSRVDLFNGLLGPVQVTALYVTRLDNVTVAHMGTMDGRILQVELVRSLNYLLYVSNFS
  LGDSGQPVQRDVSRLGDHLLFASGDQVFQVPIQGPGCRHFLTCGRCLRAWHFMGCGWCGNMCGQQKECPG
  SWQQDHCPPKLTEFHPHSGPLRGSTRLTLCGSNFYLHPSGLVPEGTHQVTVGQSPCRPLPKDSSKLRPVP
  RKDFVEEFECELEPLGTQAVGPTNVSLTVTNMPPGKHFRVDGTSVLRGFSFMEPVLIAVQPLFGPRAGGT
  CLTLEGQSLSVGTSRAVLVNGTECLLARVSEGQLLCATPPGATVASVPLSLQVGGAQVPGSWTFQYREDP
  VVLSISPNCGYINSHITICGQHLTSAWHLVLSFHDGLRAVESRQCERQLPEQQLCRLPEYVVRDPQGWVA
  GNLSARGDGAAGFTLPGFRFLPPPHPPSANLVPLKPEEHAIKFEYIGLGAVADCVGINVTVGGESCQHEF
  RGDMVVCPLPPSLQLGQDGAPLQVCVDGECHILGRVVRPGPDGVPQSTLLGILLPLLLLVAALATALVFS
  YWWRRKQLVLPPNLNDLASLDQTAGATPLPILYSGSDYRSGLALPAIDGLDSTTCVHGASFSDSEDESCV
  PLLRKESIQLRDLDSALLAEVKDVLIPHERVVTHSDRVIGKGHFGVVYHGEYIDQAQNRIQCAIKSLSRI
  TEMQQVEAFLREGLLMRGLNHPNVLALIGIMLPPEGLPHVLLPYMCHGDLLQFIRSPQRNPTVKDLISFG
  LQVARGMEYLAEQKFVHRDLAARNCMLDESFTVKVADFGLARDILDREYYSVQQHRHARLPVKWMALESL
  QTYRFTTKSDVWSFGVLLWELLTRGAPPYRHIDPFDLTHFLAQGRRLPQPEYCPDSLYQVMQQCWEADPA
  VRPTFRVLVGEVEQIVSALLGDHYVQLPATYMNLGPSTSHEMNVRPEQPQFSPMPGNVRRPRPLSEPPRP
  T
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002447
• ORF Size: 4203 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002447.4
• RefSeq Size: 4541 bp
• RefSeq ORF: 4203 bp
• Locus ID: 4486
• UniProt ID: Q04912
• Cytogenetics: 3p21.31
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transmembrane
• MW: 152.37 kDa
• Gene Summary: This gene encodes a cell surface receptor for macrophage-stimulating protein (MSP) with tyrosine kinase activity. The mature form of this protein is a heterodimer of disulfide-linked alpha and beta subunits, generated by proteolytic cleavage of a single-chain precursor. The beta subunit undergoes tyrosine phosphorylation upon stimulation by MSP. This protein is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung, and together with MSP, thought to be involved in host defense. Alternative splicing generates multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Jan 2016]