CACNA1H (NM_021098) Human Tagged ORF Clone

CAT#: RC212772

CACNA1H (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, T type, alpha 1H subunit (CACNA1H), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY412088) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212772 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CACNA1H
• Synonyms: CACNA1HB; Cav3.2; ECA6; EIG6; HALD4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212772 representing NM_021098
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGCGCCAGATCTC
  AAGCTTAACTAGCTAGCGGACCGAC
  
  ATGACCGAGGGCGCACGGGCCGCCGACGAGGTCCGGGTGCCCCTGGGCGCGCCGCCCCCTGGCCCTGCGG
  CGTTGGTGGGGGCGTCCCCGGAGAGCCCCGGGGCGCCGGGACGCGAGGCGGAGCGGGGGTCCGAGCTCGG
  CGTGTCACCCTCCGAGAGCCCGGCGGCCGAGCGCGGCGCGGAGCTGGGTGCCGACGAGGAGCAGCGCGTC
  CCGTACCCGGCCTTGGCGGCCACGGTCTTCTTCTGCCTCGGTCAGACCACGCGGCCGCGCAGCTGGTGCC
  TCCGGCTGGTCTGCAACCCATGGTTCGAGCACGTGAGCATGCTGGTAATCATGCTCAACTGCGTGACCCT
  GGGCATGTTCCGGCCCTGTGAGGACGTTGAGTGCGGCTCCGAGCGCTGCAACATCCTGGAGGCCTTTGAC
  GCCTTCATTTTCGCCTTTTTTGCGGTGGAGATGGTCATCAAGATGGTGGCCTTGGGGCTGTTCGGGCAGA
  AGTGTTACCTGGGTGACACGTGGAACAGGCTGGATTTCTTCATCGTCGTGGCGGGCATGATGGAGTACTC
  GTTGGACGGACACAACGTGAGCCTCTCGGCTATCAGGACCGTGCGGGTGCTGCGGCCCCTCCGCGCCATC
  AACCGCGTGCCTAGCATGCGGATCCTGGTCACTCTGCTGCTGGATACGCTGCCCATGCTCGGGAACGTCC
  TTCTGCTGTGCTTCTTCGTCTTCTTCATTTTCGGCATCGTTGGCGTCCAGCTCTGGGCTGGCCTCCTGCG
  GAACCGCTGCTTCCTGGACAGTGCCTTTGTCAGGAACAACAACCTGACCTTCCTGCGGCCGTACTACCAG
  ACGGAGGAGGGCGAGGAGAACCCGTTCATCTGCTCCTCACGCCGAGACAACGGCATGCAGAAGTGCTCGC
  ACATCCCCGGCCGCCGCGAGCTGCGCATGCCCTGCACCCTGGGCTGGGAGGCCTACACGCAGCCGCAGGC
  CGAGGGGGTGGGCGCTGCACGCAACGCCTGCATCAACTGGAACCAGTACTACAACGTGTGCCGCTCGGGT
  GACTCCAACCCCCACAACGGTGCCATCAACTTCGACAACATCGGCTACGCCTGGATTGCCATCTTCCAGG
  TGATCACGCTGGAAGGCTGGGTGGACATCATGTACTACGTCATGGACGCCCACTCATTCTACAACTTCAT
  CTATTTCATCCTGCTCATCATCGTGGGCTCCTTCTTCATGATCAACCTGTGCCTGGTGGTGATTGCCACG
  CAGTTCTCGGAGACGAAGCAGCGGGAGAGTCAGCTGATGCGGGAGCAGCGGGCACGCCACCTGTCCAACG
  ACAGCACGCTGGCCAGCTTCTCCGAGCCTGGCAGCTGCTACGAAGAGCTGCTGAAGTACGTGGGCCACAT
  ATTCCGCAAGGTCAAGCGGCGCAGCTTGCGCCTCTACGCCCGCTGGCAGAGCCGCTGGCGCAAGAAGGTG
  GACCCCAGTGCTGTGCAAGGCCAGGGTCCCGGGCACCGCCAGCGCCGGGCAGGCAGGCACACAGCCTCGG
  TGCACCACCTGGTTTACCACCACCATCACCACCACCACCACCACTACCATTTCAGCCATGGCAGCCCCCG
  CAGGCCCGGCCCCGAGCCAGGCGCCTGCGACACCAGGCTGGTCCGAGCTGGCGCGCCCCCCTCGCCACCT
  TCCCCAGGCCGCGGACCCCCCGACGCAGAGTCTGTGCACAGCATCTACCATGCCGACTGCCACATAGAGG
  GGCCGCAGGAGAGGGCCCGGGTGGCACATGCCGCAGCCACTGCCGCTGCCAGCCTCAGACTGGCCACAGG
  GCTGGGCACCATGAACTACCCCACGATCCTGCCCTCAGGGGTGGGCAGCGGCAAAGGCAGCACCAGCCCC
  GGACCCAAGGGGAAGTGGGCCGGTGGACCGCCAGGCACCGGGGGGCACGGCCCGTTGAGCTTGAACAGCC
  CTGATCCCTACGAGAAGATCCCGCATGTGGTCGGGGAGCATGGACTGGGCCAGGCCCCTGGCCATCTGTC
  GGGCCTCAGTGTGCCCTGCCCCCTGCCCAGCCCCCCAGCGGGCACACTGACCTGTGAGCTGAAGAGCTGC
  CCGTACTGCACCCGTGCCCTGGAGGACCCGGAGGGTGAGCTCAGCGGCTCGGAAAGTGGAGACTCAGATG
  GCCGTGGCGTCTATGAATTCACGCAGGACGTCCGGCACGGTGACCGCTGGGACCCCACGCGACCACCCCG
  TGCGACGGACACACCAGGCCCAGGCCCAGGCAGCCCCCAGCGGCGGGCACAGCAGAGGGCAGCCCCGGGC
  GAGCCAGGCTGGATGGGCCGCCTCTGGGTTACCTTCAGCGGCAAGCTGCGCCGCATCGTGGACAGCAAGT
  ACTTCAGCCGTGGCATCATGATGGCCATCCTTGTCAACACGCTGAGCATGGGCGTGGAGTACCATGAGCA
  GCCCGAGGAGCTGACTAATGCTCTGGAGATCAGCAACATCGTGTTCACCAGCATGTTTGCCCTGGAGATG
  CTGCTGAAGCTGCTGGCCTGCGGCCCTCTGGGCTACATCCGGAACCCGTACAACATCTTCGACGGCATCA
  TCGTGGTCATCAGCGTCTGGGAGATCGTGGGGCAGGCGGACGGTGGCTTGTCTGTGCTGCGCACCTTCCG
  GCTGCTGCGTGTGCTGAAGCTGGTGCGCTTTCTGCCAGCCCTGCGGCGCCAGCTCGTGGTGCTGGTGAAG
  ACCATGGACAACGTGGCTACCTTCTGCACGCTGCTCATGCTCTTCATTTTCATCTTCAGCATCCTGGGCA
  TGCACCTTTTCGGCTGCAAGTTCAGCCTGAAGACAGACACCGGAGACACCGTGCCTGACAGGAAGAACTT
  CGACTCCCTGCTGTGGGCCATCGTCACCGTGTTCCAGATCCTGACCCAGGAGGACTGGAACGTGGTCCTG
  TACAACGGCATGGCCTCCACCTCCTCCTGGGCCGCCCTCTACTTCGTGGCCCTCATGACCTTCGGCAACT
  ATGTGCTCTTCAACCTGCTGGTGGCCATCCTCGTGGAGGGCTTCCAGGCGGAGGGCGATGCCAACAGATC
  CGACACGGACGAGGACAAGACGTCGGTCCACTTCGAGGAGGACTTCCACAAGCTCAGAGAACTCCAGACC
  ACAGAGCTGAAGATGTGTTCCCTGGCCGTGACCCCCAACGGGCACCTGGAGGGACGAGGCAGCCTGTCCC
  CTCCCCTCATCATGTGCACAGCTGCCACGCCCATGCCTACCCCCAAGAGCTCACCATTCCTGGATGCAGC
  CCCCAGCCTCCCAGACTCTCGGCGTGGCAGCAGCAGCTCCGGGGACCCGCCACTGGGAGACCAGAAGCCT
  CCGGCCAGCCTCCGAAGTTCTCCCTGTGCCCCCTGGGGCCCCAGTGGCGCCTGGAGCAGCCGGCGCTCCA
  GCTGGAGCAGCCTGGGCCGTGCCCCCAGCCTCAAGCGCCGCGGCCAGTGTGGGGAACGTGAGTCCCTGCT
  GTCTGGCGAGGGCAAGGGCAGCACCGACGACGAAGCTGAGGACGGCAGGGCCGCGCCCGGGCCCCGTGCC
  ACCCCACTGCGGCGGGCCGAGTCCCTGGACCCACGGCCCCTGCGGCCGGCCGCCCTCCCGCCTACCAAGT
  GCCGCGATCGCGACGGGCAGGTGGTGGCCCTGCCCAGCGACTTCTTCCTGCGCATCGACAGCCACCGTGA
  GGATGCAGCCGAGCTTGACGACGACTCGGAGGACAGCTGCTGCCTCCGCCTGCATAAAGTGCTGGAGCCC
  TACAAGCCCCAGTGGTGCCGGAGCCGCGAGGCCTGGGCCCTCTACCTCTTCTCCCCACAGAACCGGTTCC
  GCGTCTCCTGCCAGAAGGTCATCACACACAAGATGTTTGATCACGTGGTCCTCGTCTTCATCTTCCTCAA
  CTGCGTCACCATCGCCCTGGAGAGGCCTGACATTGACCCCGGCAGCACCGAGCGGGTCTTCCTCAGCGTC
  TCCAATTACATCTTCACGGCCATCTTCGTGGCAGAGATGATGGTGAAGGTGGTGGCCCTGGGGCTGCTGT
  CCGGCGAGCACGCCTACCTGCAGAGCAGCTGGAACCTGCTGGATGGGCTGCTGGTGCTGGTGTCCCTGGT
  GGACATTGTCGTGGCCATGGCCTCGGCTGGTGGCGCCAAGATCCTGGGTGTTCTGCGCGTGCTGCGTCTG
  CTGCGGACCCTGCGGCCTCTGAGGGTCATCAGCCGGGCCCCGGGCCTCAAGCTGGTGGTGGAGACGCTGA
  TATCATCACTCAGGCCCATTGGGAACATCGTCCTCATCTGCTGCGCCTTCTTCATCATTTTTGGCATCTT
  GGGTGTGCAGCTCTTCAAAGGGAAGTTCTACTACTGCGAGGGCCCCGACACCAGGAACATCTCCACCAAG
  GCACAGTGCCGGGCCGCCCACTACCGCTGGGTGCGACGCAAGTACAACTTCGACAACCTGGGCCAGGCCC
  TGATGTCGCTGTTCGTGCTGTCATCCAAGGATGGATGGGTGAACATCATGTACGACGGGCTGGATGCCGT
  GGGTGTCGACCAGCAGCCTGTGCAGAACCACAACCCCTGGATGCTGCTGTACTTCATCTCCTTCCTGCTC
  ATCGTCAGCTTCTTCGTGCTCAACATGTTCGTGGGCGTCGTGGTCGAGAACTTCCACAAGTGCCGGCAGC
  ACCAGGAGGCGGAGGAGGCGCGGCGGCGAGAGGAGAAGCGGCTGCGGCGCCTAGAGAGGAGGCGCAGGAG
  CACTTTCCCCAGCCCAGAGGCCCAGCGCCGGCCCTACTATGCCGACTACTCGCCCACGCGCCGCTCCATT
  CACTCGCTGTGCACCAGCCACTATCTCGACCTCTTCATCACCTTCATCATCTGTGTCAACGTCATCACCA
  TGTCCATGGAGCACTATAACCAACCCAAGTCGCTGGACGAGGCCCTCAAGTACTGCAACTACGTCTTCAC
  CATCGTGTTTGTCTTCGAGGCTGCACTGAAGCTGGTAGCATTTGGGTTCCGTCGGTTCTTCAAGGACAGG
  TGGAACCAGCTGGACCTGGCCATCGTGCTGCTGTCACTCATGGGCATCACGCTGGAGGAGATAGAGATGA
  GCGCCGCGCTGCCCATCAACCCCACCATCATCCGCATCATGCGCGTGCTTCGCATTGCCCGTGTGCTGAA
  GCTGCTGAAGATGGCTACGGGCATGCGCGCCCTGCTGGACACTGTGGTGCAAGCTCTCCCCCAGGTGGGG
  AACCTGGGCCTTCTTTTCATGCTCCTGTTTTTTATCTATGCTGCGCTGGGAGTGGAGCTGTTCGGGAGGC
  TGGAGTGCAGTGAAGACAACCCCTGCGAGGGCCTGAGCAGGCACGCCACCTTCAGCAACTTCGGCATGGC
  CTTCCTCACGCTGTTCCGCGTGTCCACGGGGGACAACTGGAACGGGATCATGAAGGACACGCTGCGCGAG
  TGCTCCCGTGAGGACAAGCACTGCCTGAGCTACCTGCCGGCCCTGTCGCCCGTCTACTTCGTGACCTTCG
  TGCTGGTGGCCCAGTTCGTGCTGGTGAACGTGGTGGTGGCCGTGCTCATGAAGCACCTGGAGGAGAGCAA
  CAAGGAGGCACGGGAGGATGCGGAGCTGGACGCCGAGATCGAGCTGGAGATGGCGCAGGGCCCCGGGAGT
  GCACGCCGGGTGGACGCGGACAGGCCTCCCTTGCCCCAGGAGAGTCCGGGCGCCAGGGATGCCCCAAACC
  TGGTTGCACGCAAGGTGTCCGTGTCCAGGATGCTCTCGCTGCCCAACGACAGCTACATGTTCAGGCCCGT
  GGTGCCTGCCTCGGCGCCCCACCCCCGCCCGCTGCAGGAGGTGGAGATGGAGACCTATGGGGCCGGCACC
  CCCTTGGGCTCCGTTGCCTCTGTGCACTCTCCGCCCGCAGAGTCCTGTGCCTCCCTCCAGATCCCACTGG
  CTGTGTCGTCCCCAGCCAGGAGCGGCGAGCCCCTCCACGCCCTGTCCCCTCGGGGCACAGCCCGCTCCCC
  CAGTCTCAGCCGGCTGCTCTGCAGACAGGAGGCTGTGCACACCGATTCCTTGGAAGGGAAGATTGACAGC
  CCTAGGGACACCCTGGATCCTGCAGAGCCTGGTGAGAAAACCCCGGTGAGGCCGGTGACCCAGGGGGGCT
  CCCTGCAGTCCCCACCACGCTCCCCACGGCCCGCCAGCGTCCGCACTCGTAAGCATACCTTCGGACAGCG
  CTGCGTCTCCAGCCGGCCGGCGGCCCCAGGCGGAGAGGAGGCCGAGGCCTCGGACCCAGCCGACGAGGAG
  GTCAGCCACATCACCAGCTCCGCCTGCCCCTGGCAGCCCACAGCCGAGCCCCATGGCCCCGAAGCCTCTC
  CGGTGGCCGGCGGCGAGCGGGACCTGCGCAGGCTCTACAGCGTGGACGCTCAGGGCTTCCTGGACAAGCC
  GGGCCGGGCAGACGAGCAGTGGCGGCCCTCGGCGGAGCTGGGCAGCGGGGAGCCTGGGGAGGCGAAGGCC
  TGGGGCCCTGAGGCCGAGCCCGCTCTGGGTGCGCGCAGAAAGAAGAAGATGAGCCCCCCCTGCATCTCGG
  TGGAACCCCCTGCGGAGGACGAGGGCTCTGCGCGGCCCTCCGCGGCAGAGGGCGGCAGCACCACACTGAG
  GCGCAGGACCCCGTCCTGTGAGGCCACGCCTCACAGGGACTCCCTGGAGCCCACAGAGGGCTCAGGCGCC
  GGGGGGGACCCTGCAGCCAAGGGGGAGCGCTGGGGCCAGGCCTCCTGCCGGGCTGAGCACCTGACCGTCC
  CCAGCTTTGCCTTTGAGCCGCTGGACCTCGGGGTCCCCAGTGGAGACCCTTTCTTGGACGGTAGCCACAG
  TGTGACCCCAGAATCCAGAGCTTCCTCTTCAGGGGCCATAGTGCCCCTGGAACCCCCAGAATCAGAGCCT
  CCCATGCCCGTCGGTGACCCCCCAGAGAAGAGGCGGGGGCTGTACCTCACAGTCCCCCAGTGTCCTCTGG
  AGAAACCAGGGTCCCCCTCAGCCACCCCTGCCCCAGGGGGTGGTGCAGATGACCCCGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212772 representing NM_021098
  Red=Cloning site Green=Tags(s)
  
  MTEGARAADEVRVPLGAPPPGPAALVGASPESPGAPGREAERGSELGVSPSESPAAERGAELGADEEQRV
  PYPALAATVFFCLGQTTRPRSWCLRLVCNPWFEHVSMLVIMLNCVTLGMFRPCEDVECGSERCNILEAFD
  AFIFAFFAVEMVIKMVALGLFGQKCYLGDTWNRLDFFIVVAGMMEYSLDGHNVSLSAIRTVRVLRPLRAI
  NRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDSAFVRNNNLTFLRPYYQ
  TEEGEENPFICSSRRDNGMQKCSHIPGRRELRMPCTLGWEAYTQPQAEGVGAARNACINWNQYYNVCRSG
  DSNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIAT
  QFSETKQRESQLMREQRARHLSNDSTLASFSEPGSCYEELLKYVGHIFRKVKRRSLRLYARWQSRWRKKV
  DPSAVQGQGPGHRQRRAGRHTASVHHLVYHHHHHHHHHYHFSHGSPRRPGPEPGACDTRLVRAGAPPSPP
  SPGRGPPDAESVHSIYHADCHIEGPQERARVAHAAATAAASLRLATGLGTMNYPTILPSGVGSGKGSTSP
  GPKGKWAGGPPGTGGHGPLSLNSPDPYEKIPHVVGEHGLGQAPGHLSGLSVPCPLPSPPAGTLTCELKSC
  PYCTRALEDPEGELSGSESGDSDGRGVYEFTQDVRHGDRWDPTRPPRATDTPGPGPGSPQRRAQQRAAPG
  EPGWMGRLWVTFSGKLRRIVDSKYFSRGIMMAILVNTLSMGVEYHEQPEELTNALEISNIVFTSMFALEM
  LLKLLACGPLGYIRNPYNIFDGIIVVISVWEIVGQADGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLVK
  TMDNVATFCTLLMLFIFIFSILGMHLFGCKFSLKTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVL
  YNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSDTDEDKTSVHFEEDFHKLRELQT
  TELKMCSLAVTPNGHLEGRGSLSPPLIMCTAATPMPTPKSSPFLDAAPSLPDSRRGSSSSGDPPLGDQKP
  PASLRSSPCAPWGPSGAWSSRRSSWSSLGRAPSLKRRGQCGERESLLSGEGKGSTDDEAEDGRAAPGPRA
  TPLRRAESLDPRPLRPAALPPTKCRDRDGQVVALPSDFFLRIDSHREDAAELDDDSEDSCCLRLHKVLEP
  YKPQWCRSREAWALYLFSPQNRFRVSCQKVITHKMFDHVVLVFIFLNCVTIALERPDIDPGSTERVFLSV
  SNYIFTAIFVAEMMVKVVALGLLSGEHAYLQSSWNLLDGLLVLVSLVDIVVAMASAGGAKILGVLRVLRL
  LRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGPDTRNISTK
  AQCRAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGVDQQPVQNHNPWMLLYFISFLL
  IVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLERRRRSTFPSPEAQRRPYYADYSPTRRSI
  HSLCTSHYLDLFITFIICVNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDR
  WNQLDLAIVLLSLMGITLEEIEMSAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVG
  NLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFSNFGMAFLTLFRVSTGDNWNGIMKDTLRE
  CSREDKHCLSYLPALSPVYFVTFVLVAQFVLVNVVVAVLMKHLEESNKEAREDAELDAEIELEMAQGPGS
  ARRVDADRPPLPQESPGARDAPNLVARKVSVSRMLSLPNDSYMFRPVVPASAPHPRPLQEVEMETYGAGT
  PLGSVASVHSPPAESCASLQIPLAVSSPARSGEPLHALSPRGTARSPSLSRLLCRQEAVHTDSLEGKIDS
  PRDTLDPAEPGEKTPVRPVTQGGSLQSPPRSPRPASVRTRKHTFGQRCVSSRPAAPGGEEAEASDPADEE
  VSHITSSACPWQPTAEPHGPEASPVAGGERDLRRLYSVDAQGFLDKPGRADEQWRPSAELGSGEPGEAKA
  WGPEAEPALGARRKKKMSPPCISVEPPAEDEGSARPSAAEGGSTTLRRRTPSCEATPHRDSLEPTEGSGA
  GGDPAAKGERWGQASCRAEHLTVPSFAFEPLDLGVPSGDPFLDGSHSVTPESRASSSGAIVPLEPPESEP
  PMPVGDPPEKRRGLYLTVPQCPLEKPGSPSATPAPGGGADDPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: RsrII-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021098
• ORF Size: 7059 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021098.3
• RefSeq Size: 8097 bp
• RefSeq ORF: 7062 bp
• Locus ID: 8912
• UniProt ID: O95180
• Cytogenetics: 16p13.3
• Protein Families: Druggable Genome, Ion Channels: Calcium, Transmembrane
• Protein Pathways: Calcium signaling pathway, MAPK signaling pathway
• MW: 259 kDa
• Gene Summary: This gene encodes a T-type member of the alpha-1 subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. The alpha-1 subunit has 24 transmembrane segments and forms the pore through which ions pass into the cell. There are multiple isoforms of each of the proteins in the complex, either encoded by different genes or the result of alternative splicing of transcripts. Alternate transcriptional splice variants, encoding different isoforms, have been characterized for the gene described here. Studies suggest certain mutations in this gene lead to childhood absence epilepsy (CAE). [provided by RefSeq, Jul 2008]