Histidine decarboxylase (HDC) (NM_002112) Human Tagged ORF Clone

CAT#: RC212698

HDC (Myc-DDK-tagged)-Human histidine decarboxylase (HDC)

Product Images

Circular map for RC212698

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  Western blot validation of overexpression lysate (Cat# LY419530) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212698 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified HDC protein (Cat# TP312698). The protein was produced from HEK293T cells transfected with HDC cDNA clone (Cat# RC212698) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Histidine decarboxylase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212698 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAGCCTGAGGAGTACAGAGAGAGAGGGAGAGAGATGGTGGATTACATCTGCCAGTACCTGAGCA
  CTGTGCGGGAGAGACGTGTGATGCCAGACGTGCAGCCTGGCTACCTGCGAGCCCAGCTGCCTGAGAGTGC
  TCCTGAGGACCCCGACAGCTGGGACAGCATCTTTGGGGACATTGAACGAATCATCATGCCTGGGGTGGTA
  CATTGGCAGAGCCCCCATATGCACGCCTACTACCCAGCCCTCACCTCTTGGCCCTCCCTGCTAGGAGACA
  TGCTGGCTGATGCCATCAACTGCTTGGGATTCACCTGGGCATCCAGCCCTGCGTGTACAGAGCTGGAGAT
  GAACGTCATGGACTGGTTGGCAAAAATGCTGGGACTTCCAGAGCACTTCTTGCACCACCACCCCAGCAGC
  CAGGGCGGAGGCGTCCTGCAGAGCACGGTCAGTGAATCCACTTTGATTGCCCTGCTGGCAGCAAGGAAGA
  ACAAAATCCTGGAAATGAAAACGTCTGAGCCCGATGCTGATGAGTCCTGCCTAAATGCCCGACTCGTGGC
  CTATGCCTCTGACCAGGCTCACTCCTCTGTGGAAAAGGCTGGTTTGATTTCCCTTGTGAAGATGAAATTT
  CTGCCTGTGGATGACAACTTCTCACTCCGAGGGGAAGCTCTTCAGAAGGCCATCGAGGAAGACAAGCAGC
  GGGGCTTGGTGCCCGTCTTTGTCTGTGCAACACTAGGGACCACTGGGGTCTGTGCATTTGACTGCCTGTC
  AGAGCTGGGCCCCATCTGTGCCCGTGAGGGGCTGTGGCTCCACATCGATGCTGCTTATGCAGGCACTGCC
  TTCCTGTGCCCCGAGTTCCGGGGGTTTCTGAAGGGGATTGAGTATGCCGACTCCTTCACCTTTAATCCTT
  CCAAGTGGATGATGGTGCATTTTGACTGTACTGGGTTCTGGGTCAAGGACAAGTACAAGCTGCAGCAGAC
  CTTCAGTGTGAATCCCATCTACCTCAGGCATGCCAACTCAGGCGTGGCCACCGACTTCATGCACTGGCAG
  ATCCCCCTGAGCCGACGGTTTCGCTCTGTTAAACTCTGGTTCGTGATTCGGTCCTTCGGGGTGAAGAATC
  TTCAAGCACATGTCAGACATGGTACTGAAATGGCTAAATATTTTGAATCTCTGGTCAGAAACGACCCTTC
  CTTTGAAATTCCTGCCAAGAGGCACCTTGGCCTGGTGGTTTTTCGTCTAAAGGGTCCTAATTGTCTCACA
  GAAAATGTGTTAAAGGAAATAGCTAAAGCTGGCCGTCTCTTCCTCATCCCGGCCACTATCCAGGACAAGT
  TAATCATCCGTTTCACTGTGACATCCCAGTTTACCACTAGGGATGACATCCTGAGAGACTGGAATCTCAT
  TCGAGATGCTGCCACTCTCATCCTGAGTCAGCACTGTACTTCCCAACCCAGCCCTCGGGTTGGGAACCTC
  ATCTCCCAAATCAGGGGTGCCAGAGCCTGGGCCTGTGGAACGTCCCTTCAGTCTGTCAGTGGGGCAGGAG
  ATGATCCAGTCCAGGCCAGGAAGATCATCAAGCAGCCTCAGCGTGTGGGAGCCGGTCCCATGAAAAGGGA
  AAATGGCCTCCATCTTGAAACCCTGCTGGACCCAGTTGATGACTGCTTTTCAGAAGAGGCCCCAGATGCC
  ACCAAGCACAAGCTGTCCTCCTTCCTGTTCAGTTACTTGTCTGTGCAGACTAAGAAGAAGACGGTGCGCT
  CCCTCAGTTGCAACAGTGTGCCAGTGAGTGCTCAGAAGCCACTGCCCACAGAGGCCTCTGTGAAGAATGG
  GGGCTCCTCCAGGGTCAGAATCTTTTCCAGGTTTCCAGAAGACATGATGATGCTGAAGAAAAGTGCCTTC
  AAAAAACTCATCAAATTCTACAGCGTCCCCAGCTTTCCTGAATGCAGCTCTCAATGTGGACTCCAGCTGC
  CCTGTTGCCCTCTGCAGGCCATGGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212698 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMEPEEYRERGREMVDYICQYLSTVRERRVMPDVQPGYLRAQLPESAPEDPDSWDSIFGDIERIIMPGVV
  HWQSPHMHAYYPALTSWPSLLGDMLADAINCLGFTWASSPACTELEMNVMDWLAKMLGLPEHFLHHHPSS
  QGGGVLQSTVSESTLIALLAARKNKILEMKTSEPDADESCLNARLVAYASDQAHSSVEKAGLISLVKMKF
  LPVDDNFSLRGEALQKAIEEDKQRGLVPVFVCATLGTTGVCAFDCLSELGPICAREGLWLHIDAAYAGTA
  FLCPEFRGFLKGIEYADSFTFNPSKWMMVHFDCTGFWVKDKYKLQQTFSVNPIYLRHANSGVATDFMHWQ
  IPLSRRFRSVKLWFVIRSFGVKNLQAHVRHGTEMAKYFESLVRNDPSFEIPAKRHLGLVVFRLKGPNCLT
  ENVLKEIAKAGRLFLIPATIQDKLIIRFTVTSQFTTRDDILRDWNLIRDAATLILSQHCTSQPSPRVGNL
  ISQIRGARAWACGTSLQSVSGAGDDPVQARKIIKQPQRVGAGPMKRENGLHLETLLDPVDDCFSEEAPDA
  TKHKLSSFLFSYLSVQTKKKTVRSLSCNSVPVSAQKPLPTEASVKNGGSSRVRIFSRFPEDMMMLKKSAF
  KKLIKFYSVPSFPECSSQCGLQLPCCPLQAMV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002112
• ORF Size: 1986 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002112.4
• RefSeq Size: 2646 bp
• RefSeq ORF: 1989 bp
• Locus ID: 3067
• UniProt ID: P19113
• Cytogenetics: 15q21.2
• Domains: pyridoxal_deC
• Protein Families: Druggable Genome
• Protein Pathways: Histidine metabolism, Metabolic pathways
• MW: 74.2 kDa
• Gene Summary: This gene encodes a member of the group II decarboxylase family and forms a homodimer that converts L-histidine to histamine in a pyridoxal phosphate dependent manner. Histamine regulates several physiologic processes, including neurotransmission, gastric acid secretion,inflamation, and smooth muscle tone.[provided by RefSeq, Aug 2010]